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Citations for Becton, Dickinson and Company :
1 - 50 of 90 citations for SARS Coronavirus Spike Glycoprotein S1 His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... spike and SARS-CoV-1 RBD were incubated with Streptavidin-APC (BD), Streptavidin-PE (BD) ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 S protein with Avi-tag was conjugated to streptavidin-BV421 (BD Pharmingen cat.# 563259) and streptavidin-AF488 (Invitrogen cat.# S11223) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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Chromosome fissions and fusions act as barriers to gene flow between Brenthis fritillary butterfliesbioRxiv - Evolutionary Biology 2022Quote: ... daphne individual (FR_BD_1329; Table S1) was used for chromatin conformation capture (HiC ...
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bioRxiv - Immunology 2021Quote: ... Spike protein conjugated to Alexa 647 and Spike protein conjugated to Alexa 488 and Brilliant Staining buffer (BD Horizon). Samples were then incubated on ice for an additional 30 minutes before they were washed twice with P2 and fixed in a final concentration of 1% paraformaldehyde for 15 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... VCAM1-BB515 (1:20, clone S1-10C9, #9093693, BD Biosciences), CD103-PerCP-Cy5.5 (1:50 ...
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bioRxiv - Genomics 2023Quote: ... CD34+ cells (gating as per Fig. S1) were FACS-sorted (BD FACSMelody ...
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bioRxiv - Immunology 2023Quote: ... p-MPM2 (Table S1) in BD Perm/WashTM buffer (BD biosciences) containing 2% FCS for 30 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: Mice were anaesthetized with isoflurane 1% to 2% v/v oxygen and immunized subcutaneously with 200 μg myelin oligodendrocyte glycoprotein 35–55 (MOG35–55) peptide (peptides & elephants) in complete Freund’s adjuvant (BD) containing 4 mg ml−1 Mycobacterium tuberculosis (BD) ...
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bioRxiv - Immunology 2021Quote: ... or A549-Spike cells in the presence of GolgiStop (BD Biosciences) in a V-bottomed 96-well plate in complete RPMI-1640 media at 37°C under 5% CO2 for 2 hours ...
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bioRxiv - Plant Biology 2022Quote: ... The constructs were then co-transformed into yeast AH109 strain and plated on SD selective media and no detectable self-activation was observed (SD -His-Leu, + 2 mM 3-AT or SD -His-Trp, + 40 mM 3-AT (for OsSHR1-BD) or 20 mM 3-AT (for OsSHR2-BD)) ...
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bioRxiv - Immunology 2022Quote: ... CD69 BV711 (Clone HI.2F3) (BD Cat. 740664), CD103 PE-CF594 (Clone M290 ...
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bioRxiv - Immunology 2023Quote: ... the ACE2/HEK293 cells were harvested and GFP expression levels determined by BD FACSCelesta™ flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... and Sample Tag barcodes were captured by BD Rhapsody beads coated with poly(T ...
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bioRxiv - Neuroscience 2023Quote: ... and Sample Tag libraries (BD Biosciences, cat. 633801) for sequencing on Illumina® sequencers ...
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bioRxiv - Molecular Biology 2023Quote: Genes encoding HBb (BD_RS00255, old locus tag: Bd0055) and HMfB (GenBank accession number M34778.1 ...
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bioRxiv - Cancer Biology 2024Quote: SV40 TAG-specific mouse monoclonal antibody (BD Bioscience 554149) primary antibody was used ...
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bioRxiv - Immunology 2019Quote: ... and Foxp3 (S1 Table) and analyzed using a Fortessa flow cytometer running FACSDiva (BD Biosciences). FSC 3.0 files were analyzed and presented with FLOWJO Software (flowjo.com).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Spike-RBD binding was measured by flow cytometry on a BD FACSCelesta (BD Biosciences) and analyzed with FlowJo software.
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bioRxiv - Microbiology 2023Quote: ... we stained cells expressing wild-type and mutant SFV glycoproteins with a mouse anti-SFV immune ascitic fluid (ATCC Cat#: VR-1247AF) or a control mouse IgG2b antibody (BD Biosciences Cat#: 557351). Staining of wild-type and mutant EEEV or SFV E3–E2–(6K/TF)–E1 was detected with a PE-conjugated donkey anti-mouse F(ab’)2 fragment (Jackson ImmunoResearch Cat# ...
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bioRxiv - Immunology 2023Quote: ... anti-CD8 (as above) and anti-CD69 (HI.2F3, BD Bioscience) or anti-IRF4 (3e4 ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... lung and SI dLNs as indicated in Figure S1 using a BD INFLUX Cell sorter (BD Biosciences). Cells were resuspended in FACS buffer and the respective DC2 subets were sorted directly into QIAzol Lysis Reagent (QIAGEN ...
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bioRxiv - Immunology 2023Quote: ... and CD45 Sample Tags to enable multiplexing (BD Bioscience, RRID: AB_2870301) and AbSeq antibodies to ICOS (AMM2072 ...
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bioRxiv - Microbiology 2024Quote: ... Cell samples were acquired on BD LSR Fortessa and data were analyzed (Figure S1) using FlowJo 10 (BD).
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bioRxiv - Immunology 2024Quote: ... and stained for surface and intracellular markers using the antibodies listed in Table S1 for flow cytometry analysis (BD FACS Canto II ...
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bioRxiv - Cell Biology 2021Quote: ... bound proteins were analyzed by western blotting using anti-His monoclonal antibodies (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... CD169+) were flow-sorted prior to infection with SARS-CoV-2 using FACSAria Fusion (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Eosinophils were labelled with sample tags (633793 BD Mouse Single-Cell Multiplexing Kit) according to the the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... cells were labelled with sample tags (633793 BD Mouse Single-Cell Multiplexing Kit) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-cells were purified from the population of Spike-binding cells by flow cytometry using a FACSMelody (BD). Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman ...
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bioRxiv - Immunology 2021Quote: ... The binding of mAbs to spikes expressing on cell surface was analyzed using flow cytometry (BD Canto Analyzer). Percent (% ...
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bioRxiv - Biophysics 2022Quote: ... The binding of mAbs to spikes expressing on cell surface was analyzed using flow cytometry (BD Canto Analyzer). Percent (% ...
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bioRxiv - Molecular Biology 2020Quote: ... Four-way cell sorting (spermatogonia, primary spermatocytes, secondary spermatocytes, round spermatids; File S1) using a FACSAria II Cell Sorter (BD Biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... 150,000 cells were sorted in early (S1) and late (S2) S-phase fractions using a Fluorescence Activated Cell Sorting system (FACS Aria Fusion, BD) in Lysis Buffer (50 mM Tris pH 8 ...
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bioRxiv - Immunology 2024Quote: ... and stained for surface and intracellular markers using the antibodies listed in Table S1 for flow cytometry analysis (BD FACS Canto II, BD FACSARIA III ...
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bioRxiv - Immunology 2023Quote: ... biotinylated SARS-CoV-2 RBD was multimerised with SAv-BV421 and SAv-PE (563259, 554061; BD Biosciences) individually ...
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bioRxiv - Immunology 2022Quote: ... along with the pre-complexed Beta variant Spike tetramer (2 μg in 100μl) coupled to PE-streptavidin (BD Biosciences). All other aspects with cell sorting ...
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bioRxiv - Bioengineering 2019Quote: ... the cell culture mixture was stained with an antibody recognizing the neural cell adhesion molecule (NCAM/CD56; BD Pharmingen; Table S1), and the myogenic progenitor (CD56+ ...
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bioRxiv - Immunology 2021Quote: ... Cells were aliquoted to a count of 1 million cells each and incubated on ice with Fc Block (BD, Table S1) at a 1:20 dilution ...
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bioRxiv - Microbiology 2020Quote: Candida strains (Supplementary Table S1) were routinely grown at 30°C in yeast peptone dextrose (YPD) media (2% peptone (BD Bacto), 2% D-glucose (Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... Cells were initially stained with fluorochrome-conjugated antibodies against surface receptors (see Table S1) in BD Brilliant stain buffer (BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: Hematopoietic cells were stained with the antibodies listed in Supp Table S1 for 20-30 min at 4°C and analyzed on a custom BD LSR Fortessa (BD Biosciences). For sorting ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293 cells transfected with ABE plasmids were collected from 6-well plate and analyzed on Becton Dickinson LSR II (BD Biosciences) to determine GFP-positive cells ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated prefusion-stabilized spike (S-2P) and spike subdomain (NTD, RBD) probes were produced as previously described79 and conjugated to streptavidin-labeled dyes (BD) to yield the following and streptavidin-conjugated B cell probes ...
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bioRxiv - Immunology 2021Quote: ... FNA samples were stained in P2 for 30 minutes on ice with biotinylated and Alexa Fluor 647 conjugated recombinant soluble Spike proteins as well as PD-1 BB515 (EH12.1, BD Horizon). Cells were then washed twice with P2 and stained with IgG BV480 (goat polyclonal ...
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bioRxiv - Epidemiology 2019Quote: ... 2011 and 2015 were available for four of these 12 counties (Dalarna-AR, Örebro-AR, Uppsala-BD and Norrbotten-BD, Table S1).7 We had data about the yearly number of CT diagnoses from all counties since 1997.11 In the model ...
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bioRxiv - Epidemiology 2019Quote: ... 2011 and 2015 were available for four of these 12 counties (Dalarna-AR, Örebro-AR, Uppsala-BD and Norrbotten-BD, Table S1).7 We had data about the yearly number of CT diagnoses from all counties since 1997.11 In the model ...
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bioRxiv - Immunology 2024Quote: ... Single-cell suspensions were stained with antibodies listed in Table S1 and acquired with a LSR Fortessa flow cytometer (BD Bioscience, US). For cell-associated autofluorescence to be removed in the indicated experiments ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™ ...