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Citations for Becton, Dickinson and Company :
351 - 400 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were sorted by their distinct proliferation peaks (peak 0, peak 1 and peak 2) on FACS-Aria and Influx Cell Sorters (BD Biosciences). Cells were kept on ice and after washing with cold PBS were immediately resuspended in Trizol for RNA extraction ...
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bioRxiv - Immunology 2022Quote: FACS-sorted Tregs were plated at 50,000 cells/well in round bottom 96 well plates pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) and soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... resuspended in 300 μl of 2% FBS in PBS with 1000 ng/μl DAPI and processed by FACS on a Melody Cell Sorter (BD Biosciences) using the BD FACSChorus software ...
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bioRxiv - Cancer Biology 2023Quote: C4-2 cells were cultured and lifted from plates by trypsinization then re-suspended in 100 mL of 50% Matrigel (BD Biosciences) and saline mixture followed by subcutaneous injection in two separate groups of castrated and non-castrated athymic nude mice (age at least 6 weeks old) ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then stained with live/dead and cell surface markers in MACS solution (Miltenyi) supplemented with 2% BSA (Miltenyi) prior to permeabilization with Cytofix/Cytoperm (BD Biosciences) and staining with intracellular markers in Perm/Wash (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... or FAK-KD cell lines were generated by injecting a total of 2×106 MDA-MB-231/Dendra2 cells resuspended in 20% collagen I (BD Biosciences) in PBS into the lower left mammary gland of SCID mice ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were dissolved in 2% FBS in PBS and flow cytometry was performed using an Accuri C6 Plus (BD Biosciences) or Citole Flow Cytometer (Beckman Coulter).
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bioRxiv - Genomics 2023Quote: ... at least 2 × 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... The proteases were inactivated by adding 10% fetal bovine serum and 2 mM EDTA and the cell disaggregated by passing them through a 40 μm nylon sieve (BD Bioscience). Single cells were then washed with 1% Bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... 0.25X and 4X concentration of CD4 T cell-depleting antibody for 30 minutes at 37°C before surface staining with CD3 (clone SP34-2, BD Pharmingen), CD4 (Clone L200 ...
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bioRxiv - Genomics 2024Quote: ... at least 2 x 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... IL-6 and IL-1β cytokines were measured in the cell culture supernatant after 2 h of co-exposure using CBA human Inflammatory cytokine kit (BD Biosciences), while chemokines CXCL8/IL-8 ...
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bioRxiv - Physiology 2024Quote: ... endothelial cells were purified by two consecutive immuno-selection procedures using magnetic beads conjugated with anti-ICAM-2 antibody (BD Pharmingen) and plated into 75 cm2 tissue culture coated flasks ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were blocked with 2% normal mouse serum and an anti-CD16/CD32 antibody (FcγR blocker, BD Pharmingen™ 553142) for 30 min on ice ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed twice in PBS + 2% FBS and stained with mouse anti-human CD45 Pe-Cy5 (BD Bioscience, clone HI30) and mouse anti-human CD107a Alexa Fluor 647 for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2024Quote: ... Stimulated and unstimulated cells were harvested and washed with 2 mL of FACS buffer and then cells were fixed with BD transcription factor fixation/perm buffer (cat #562574, BD Bioscience) for 20 minutes at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... Antibody dilutions were made in 5% (w/v) bovine serum albumin (α-GST, α-His, α-HA, α-biotin-HRP, α-MLO2) in TBST or 5% (w/v) milk (α-Gal4 BD, α-Gal4 AD ...
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained with fluorescently labeled antibodies against BCL-2 (clone Bcl-2/100, BD Bioscience), BCL-XL (clone 54H11 ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining was performed after 2% paraformaldehyde fixation and permeabilization with Perm 2 buffer (BD Biosciences) using BV711-conjugated anti-IFNγ (clone XMG1.2 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used for immunofluorescence of HeLa cells and/or primary neurons were: mouse anti-Ataxin-2 (BD Biosciences 611378, 1:250), mouse anti-beta-III-tubulin (Covance MMS-435P ...
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bioRxiv - Bioengineering 2021Quote: ... albicans fungal cells (CMM16) were inoculated from a streaked plate in 2 ml yeast extract peptone dextrose (YPD) [1% yeast extract (BD Biosciences, 212730), 2% peptone (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... The hemocytes were collected from thirty to forty shrimps at 2 hours post injection for each sorting which was performed on a FACSMelody cell sorter (BD Biosciences, USA). The fluorescence boundary was set based on detection of the shrimp hemocyte self-fluorescence without VP injection.
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bioRxiv - Immunology 2020Quote: ... IL-2 and IFN-γ concentration secreted in cell supernatant were measured using sandwich ELISA according to manufacturer’s protocol (BD Biosciences, USA). Plates were read on a Varioskan Lux multimode microplate reader (ThermoFisher ...
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bioRxiv - Pathology 2020Quote: ... Tomato-positive and -negative cells differed by ∼2 orders of magnitude in tomato fluorescence with excitation of 554nm and emission of 581nm by Influx™ cell sorter (BD Biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS+2% goat serum and cell cycle analysis was conducted using a BD FACS Calibur (BD Biosciences, UK).
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bioRxiv - Genetics 2019Quote: ... in PBS with 1 μg/ml bovine serum albumin (BSA and 2 mM Tris-HCl. Cells were analyzed on a BD LSRII flow cytometer (BD Biosciences, Inc) and data were analyzed using FlowJo software ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... cells were fixed for 10 min at room temperature with 2% Paraformaldehyde and analysed using LSRFortessa™ X-20 Cell Analyzer (BD Biosciences), LSR II Flow Cytometer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... and plated in round-bottom 96-well plates at 1-2 x 106 cells per well in complete RPMI (cRPMI) medium containing CD28 costimulatory antibody (BD Biosciences #553294) and brefeldin A ...
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bioRxiv - Cancer Biology 2024Quote: ... Positively transduced cells were selected with 2 µg/ml puromycin followed by a sterile sort for high GFP and mCherry expression (BD FACSAria II). Expression was maintained with 1 µg/ml puromycin in all experiments except where immune cells were needed.
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% raffinose (R; BD), 2% D-galactose (G ...
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bioRxiv - Neuroscience 2020Quote: ... and Sox-2 (BD-51-9007227) antibodies.
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bioRxiv - Molecular Biology 2021Quote: ... bacto agar (2% w/v; BD) and dextrose (2% w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... and peptone (2% w/v; BD). Solid YP medium contained bacto agar (2% w/v ...
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bioRxiv - Neuroscience 2019Quote: ... 2) use a blob detector (BD) to pick up cells (as the ROIs ...
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bioRxiv - Immunology 2019Quote: ... CD3 (clone SP34-2; BD Biosciences), CD19 (clone SJ25C1 ...
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bioRxiv - Immunology 2019Quote: ... CD138-PE (BD Pharmigen, 281-2), CD19-AI647 (in-house conjugated ...
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bioRxiv - Biochemistry 2021Quote: ... anti-Ataxin-2 (BD Biosciences 611378)(1:200) ...
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bioRxiv - Bioengineering 2022Quote: ... or anti-IL-2 (BD Sciences). Nanovials were incubated with antibodies for 30 minutes at room temperature on a rotator and washed three times as described above ...
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bioRxiv - Immunology 2021Quote: ... CD3 BUV805 (clone SP34-2, BD Biosciences Cat# 742053 ...
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bioRxiv - Microbiology 2020Quote: ... anti-IL-2-APC (BD Biosciences), anti-TNF-α-PE (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... anti-IgG1 (B68-2; BD Biosciences), anti-BP-1 (6C3 ...
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bioRxiv - Genetics 2021Quote: ... 2% peptone (BD P/N: 211677), and 2% dextrose (BD P/N 15530) ...
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bioRxiv - Microbiology 2022Quote: ... anti-IgD BV605 (IA6–2, BD), anti-CD27 PE/Cy7 (LG.7F9 ...
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bioRxiv - Immunology 2020Quote: ... IgD FITC IA6-2 (BD Biosciences), Ki67 AF488 B56 (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... CD3 (APC-Cy7, SP34-2, BD), CD4 (BV510 ...
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bioRxiv - Immunology 2021Quote: ... APC-Cy7 (Sp34-2, BD Biosciences), mouse anti-human CXCR5 PE-Cy7 (MU5UBEE ...
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bioRxiv - Genetics 2021Quote: ... 2% peptone (BD P/N: 211677), and 2% ethanol (Koptec P/N A06141602W) ...
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bioRxiv - Neuroscience 2021Quote: ... 2) anti-CD31 antibodies (#550274, BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... IgD-biotinylated (IA6-2, BD Bioscience), IgM-FITC (G20-127 ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 2% raffinose (R; BD), 2% D-galactose (G ...