Labshake search
Citations for Becton, Dickinson and Company :
1 - 50 of 1980 citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... spike and SARS-CoV-1 RBD were incubated with Streptavidin-APC (BD), Streptavidin-PE (BD) ...
-
bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 S protein with Avi-tag was conjugated to streptavidin-BV421 (BD Pharmingen cat.# 563259) and streptavidin-AF488 (Invitrogen cat.# S11223) ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
-
bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
-
bioRxiv - Cancer Biology 2023Quote: ... Streptavidin-Horseradish Peroxidase (HRP) (BD #557630) and AEC chromogen substrate (BD #551951 ...
-
bioRxiv - Immunology 2022Quote: ... CD169+) were flow-sorted prior to infection with SARS-CoV-2 using FACSAria Fusion (BD Biosciences).
-
bioRxiv - Immunology 2023Quote: ... biotinylated SARS-CoV-2 RBD was multimerised with SAv-BV421 and SAv-PE (563259, 554061; BD Biosciences) individually ...
-
bioRxiv - Immunology 2021Quote: ... with horseradish peroxidase(HRP)-conjugated Streptavidin (BD Biosciences) for 1 hour (h) ...
-
bioRxiv - Neuroscience 2022Quote: ... they were probed with horseradish peroxidase-Streptavidin (BD Biosciences ...
-
bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™ ...
-
bioRxiv - Immunology 2022Quote: ... 2% Fc block (BD), and fixable viability dye eFluor 450 (ebiosciences) ...
-
bioRxiv - Physiology 2020Quote: ... and horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences, 554066; 1:300). The HRP activity was visualized with diaminobenzidine staining ...
-
bioRxiv - Immunology 2020Quote: ... and then detected with streptavidin conjugated horseradish peroxidase (554066, BD biosciences). Plates were developed using TMB (Dako ...
-
bioRxiv - Immunology 2022Quote: ... A total of 100 μl per well of horseradish peroxidase-conjugated streptavidin (BD Biosciences) diluted in PBS containing 0.1% BSA was then added and incubated for 1 hr at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... 2% FCS and 10% Brilliant Stain Buffer (BD) were then added directly to cells and incubated for a further 20 minutes at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... Immunoglobulins were detected with horseradish peroxidase (HRP) goat anti-mouse Ig (BD Pharmingen, 1/2000 diluted), IgM ...
-
bioRxiv - Immunology 2019Quote: ... followed by streptavidin-conjugated horseradish peroxidase (SA-HRP) and TMB substrate (both BD Bioscience, San Diego, CA). Anti-mouse Ig(H+L ...
-
bioRxiv - Immunology 2023Quote: ... and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG secondary Ab (BD Biosciences, San Diego, CA, USA). Chemiluminescence detection was performed with the ECL Prime Western Blotting System and acquired by the ChemiDoc Imaging System (Bio-Rad).
-
bioRxiv - Immunology 2022Quote: ... and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG secondary Ab (BD Biosciences, San Diego, CA, USA). Chemiluminescence detection was performed with the ECL Prime Western Blotting System and acquired by the ChemiDoc Imaging System (Bio-Rad).
-
bioRxiv - Microbiology 2020Quote: ... fixed with 2% paraformaldehyde with Fc block (BD Biosciences) before and after ...
-
bioRxiv - Immunology 2023Quote: ... afterward kept in staining buffer (2% FCS, BD Biosciences). Surface proteins were stained for 30 minutes at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... Cells were aliquoted to a count of 1 million cells each and incubated on ice with Fc Block (BD, Table S1) at a 1:20 dilution ...
-
bioRxiv - Immunology 2021Quote: ... IL-1β was detected using biotinylated anti IL-1β (eBioscience) and streptavidin conjugated to horseradish peroxidase (BD Biosciences). Peroxidase activity was detected using an o-phenylenediamine hydrochloride (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2020Quote: SVC were resuspended in 2% Fc Block (553142, BD Pharmingen) and blocked for 30 minutes ...
-
bioRxiv - Immunology 2022Quote: ... 2*106 cells were incubated with Fc block (BD Pharmingen) and subsequently stained for surface markers with fluorophore-conjugated antibodies for 30 minutes on ice.
-
bioRxiv - Cancer Biology 2023Quote: ... in PBS supplemented with 2% FCS and iMag (BD Biosciences). After depletion ...
-
bioRxiv - Immunology 2022Quote: ... followed by washing and incubation for 30 mins at room temperature with horseradish peroxidase (HRP)-streptavidin (BD, Cat. 554066) and Amplex red substrate for 45 mins at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... After incubation with appropriate horseradish peroxidase-conjugated secondary antibodies (HRP-linked anti-mouse or anti-rabbit IgG; BD Biosciences) at RT for 1 h ...
-
Chromosome fissions and fusions act as barriers to gene flow between Brenthis fritillary butterfliesbioRxiv - Evolutionary Biology 2022Quote: ... daphne individual (FR_BD_1329; Table S1) was used for chromatin conformation capture (HiC ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2 µL of CD16/CD32 Fc Block antibody (BD Biosciences 553141) was added for 10 minutes at 4°C in the dark ...
-
bioRxiv - Immunology 2021Quote: ... and suspended in 2% FBS/DPBS buffer with Fc Block (BD) for 10 min ...
-
bioRxiv - Immunology 2021Quote: ... IL-1β was then detected with a secondary biotinylated antibody (eBioscience) followed by streptavidin conjugated to horseradish peroxidase (BD Biosciences). Peroxidase activity was detected by o-phenylenediamine hydrochloride (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1) after Fc blocking and analyzed using flow cytometry (BD FACS Celesta/Novocyte Advanteon). Cells were fixed and permeabilized using intracellular staining reagents (Intracellular Fixation & Permeabilization Buffer Set ...
-
bioRxiv - Immunology 2021Quote: Cells resuspended in cold staining buffer (2% FCS in PBS) were blocked with mouse Fc Block (BD Biosciences, catalogue# 553141) for 10 minutes at 4°C and stained with a panel of antibodies (Supplementary Table 5) ...
-
bioRxiv - Immunology 2023Quote: ... Staining was quenched by washing in FACS buffer (PBS with 2 mM EDTA and 2% FCS) after which cells were incubated with anti-CD16/32 (Fc block, clone 2.4G2, BD Biosciences) for 15 minutes at 4°C to prevent non-specific binding ...
-
bioRxiv - Immunology 2020Quote: ... Biotinylated control peptides were exchanged with HLA-DR at 1:2 molar ratio in the presence of 5 times excess of a test peptide and detected using horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences). PBMCs obtained after vaccination from each donor were stimulated with 47 DR-binding peptides at 0.4ug/ml per peptide for 3 weeks and stained with tetramers ...
-
bioRxiv - Immunology 2021Quote: ... + 2% FBS with Fc block (1:100 dilution, clone 2.4G2; BD Biosciences) along with the following antibodies (all from BD Biosciences) ...
-
bioRxiv - Immunology 2023Quote: ... Tissues were blocked with 2% Fc block (BD Pharmingen Cat. No. 553142) with 5% normal goat serum in 1X PBS at room temperature for one hour ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice in PBS + 2% FCS + 2 mM EDTA and incubated with mouse FcBlock (BD Biosciences), Epcam-APC (CD326 Monoclonal Antibody (G8.8) ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were resuspended in PBS + 2% FCS and an Influx cell sorter (BD) was used.
-
bioRxiv - Immunology 2021Quote: ... and cells were suspended in 2% FBS/DPBS buffer with Fc Block (BD) for 10 min ...
-
bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 infected cells were fixed in 2% formaldehyde for 30 mins and permeabilized with BD Perm/Wash buffer (BD Biosciences, 554723). The primary antibody used was sheep anti-SARS-CoV-2 nucleocapsid antibody (DA114 ...
-
bioRxiv - Microbiology 2022Quote: ... and cells were suspended in 2% FBS/DPBS buffer with Fc Block (BD Biosciences) for 10 minutes15 ...
-
bioRxiv - Bioengineering 2023Quote: ... 2×105 cells were incubated with 50 µL Fc Block (BD Biosciences, cat #553142) that was pre-diluted 1:50 in FACS buffer (PBS (Gibco/Thermofisher scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... 1-2 x 106 BM cells were incubated with human Fc BlockTM (BD, 564219) for 10 minutes at RT ...
-
bioRxiv - Immunology 2023Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...
-
bioRxiv - Immunology 2020Quote: ... Samples were then washed with 2% and stained with 2% FCS in PBS and subsequently washed stained with goat anti-rabbit IgG –FITC secondary (BD) for 30 minutes at RT ...
-
bioRxiv - Immunology 2022Quote: ... along with the pre-complexed Beta variant Spike tetramer (2 μg in 100μl) coupled to PE-streptavidin (BD Biosciences). All other aspects with cell sorting ...
-
bioRxiv - Cell Biology 2021Quote: ... sheep anti-human CD31 1:500 (BD AF806) and chicken anti-GFP 1:1000 (Abcam ab13970) ...