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Citations for Becton, Dickinson and Company :
1 - 50 of 1206 citations for SARS CoV 2 Nucleoprotein His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 S protein with Avi-tag was conjugated to streptavidin-BV421 (BD Pharmingen cat.# 563259) and streptavidin-AF488 (Invitrogen cat.# S11223) ...
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bioRxiv - Immunology 2022Quote: ... CD169+) were flow-sorted prior to infection with SARS-CoV-2 using FACSAria Fusion (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... biotinylated SARS-CoV-2 RBD was multimerised with SAv-BV421 and SAv-PE (563259, 554061; BD Biosciences) individually ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™ ...
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bioRxiv - Immunology 2022Quote: ... spike and SARS-CoV-1 RBD were incubated with Streptavidin-APC (BD), Streptavidin-PE (BD) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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bioRxiv - Plant Biology 2022Quote: ... The constructs were then co-transformed into yeast AH109 strain and plated on SD selective media and no detectable self-activation was observed (SD -His-Leu, + 2 mM 3-AT or SD -His-Trp, + 40 mM 3-AT (for OsSHR1-BD) or 20 mM 3-AT (for OsSHR2-BD)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 infected cells were fixed in 2% formaldehyde for 30 mins and permeabilized with BD Perm/Wash buffer (BD Biosciences, 554723). The primary antibody used was sheep anti-SARS-CoV-2 nucleocapsid antibody (DA114 ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...
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bioRxiv - Immunology 2020Quote: ... In experiments testing double recombinants SARS-CoV2 vectors IL-2 antibody was not included and PE-CF594 anti-mouse IL-4 (clone 11B11, 562450, BD) and BV421 rat anti mouse IL-10 (clone JES5-16E3 ...
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bioRxiv - Cell Biology 2023Quote: Cytokine released by the SARS-CoV-2-infected or ApoBD-treated MDMs were measured using human cytokine bead array as per manufacturer’s instruction (BD Biosciences). Briefly ...
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bioRxiv - Immunology 2021Quote: Cultured T cells were stimulated with the cognate SARS-CoV-2 OPPs (ORF1ab-C, ORF3a, N, S, nsp12) and incubated for 4 hours in the presence of Golgiplug (BD Biosciences). Following stimulation ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 enriched or unenriched cells were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). From infant thymus and PBMC donors up to 5 populations were sorted after doublet ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then washed with FC buffer (PBS supplemented with 2% HI-FBS and 0.05% NaN3) and resuspended in Fixation Buffer (BD Pharmingen)for 5 min at 4C° in the dark ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were blocked for 10 min in ice-cold PBS+2% heat-inactivated FBS (HI-FBS) containing anti-CD16/CD32 (BD Biosciences) and rat-γ-globulin (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2021Quote: ... the ELISA was performed by coating 96 well plates with 2 µg/mL LRRC15-His (AcroBio, #LR5-H52H3) in coating buffer (BD, #51-2713KC) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... CD69 BV711 (Clone HI.2F3) (BD Cat. 740664), CD103 PE-CF594 (Clone M290 ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... the ACE2/HEK293 cells were harvested and GFP expression levels determined by BD FACSCelesta™ flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... and Sample Tag barcodes were captured by BD Rhapsody beads coated with poly(T ...
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bioRxiv - Neuroscience 2023Quote: ... and Sample Tag libraries (BD Biosciences, cat. 633801) for sequencing on Illumina® sequencers ...
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bioRxiv - Molecular Biology 2023Quote: Genes encoding HBb (BD_RS00255, old locus tag: Bd0055) and HMfB (GenBank accession number M34778.1 ...
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bioRxiv - Cancer Biology 2024Quote: SV40 TAG-specific mouse monoclonal antibody (BD Bioscience 554149) primary antibody was used ...
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bioRxiv - Immunology 2023Quote: ... anti-CD8 (as above) and anti-CD69 (HI.2F3, BD Bioscience) or anti-IRF4 (3e4 ...
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bioRxiv - Immunology 2023Quote: ... and CD45 Sample Tags to enable multiplexing (BD Bioscience, RRID: AB_2870301) and AbSeq antibodies to ICOS (AMM2072 ...
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bioRxiv - Cell Biology 2021Quote: ... bound proteins were analyzed by western blotting using anti-His monoclonal antibodies (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Eosinophils were labelled with sample tags (633793 BD Mouse Single-Cell Multiplexing Kit) according to the the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... cells were labelled with sample tags (633793 BD Mouse Single-Cell Multiplexing Kit) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293 cells transfected with ABE plasmids were collected from 6-well plate and analyzed on Becton Dickinson LSR II (BD Biosciences) to determine GFP-positive cells ...
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bioRxiv - Microbiology 2023Quote: ... Yersinia pestis strains were routinely grown in Heart Infusion (HI) broth (Difco, BD, Franklin Lakes, NJ) or on HI agar at 28°C ...
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bioRxiv - Immunology 2022Quote: ... an Alexa Fluor 647 AffiniPure anti-HA tag Goat Anti-Mouse IgG was used (BD Bioscience). A375 stained with anti-AXL PE-conjugated antibodies (FAB154P ...
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bioRxiv - Biochemistry 2021Quote: ... anti-ALFA tag (NanoTag Biotechnologies; cat#N1502-SC3) and anti-GM130 (BD Transduction Lab; cat#610823). Images were acquired on a Leica DM6B microscope with a 63X objectives.
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bioRxiv - Immunology 2022Quote: ... cells were stained with Sample Tag oligonucleotide-conjugated antibodies in Single-Cell Multiplexing Kit (BD Biosciences) as in Supplementary Table S6.
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bioRxiv - Molecular Biology 2020Quote: ... Pure polyclonal population of HA-tag-MEOX1 FBs were sorted by flow cytometry (BD Arial II) for stable mCherry expression ...
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bioRxiv - Immunology 2021Quote: ... The anion exchange fractions were analyzed by Western blot using anti-His antibody (BD Pharmingen; Cat#552565) and Goat poyclonal anti-mouse IgG-HRP (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2019Quote: ... the three assessed T-cell subsets were incubated with sample tag antibodies (Sample multiplexing kit; BD Bioscience), washed 3 times in cold BD sample buffer (BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... Blood samples from HI or patients were collected in 10 ml EDTA tubes (K2E, REF 367525, BD Vacutainer) for plasma or in 4 ml CAT tubes (Clot Activator Tube ...
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bioRxiv - Biochemistry 2023Quote: ... nanobodies were purified with a 96-wells HIS-TRAP plate or a Cobalt-NTA (Talon) column (BD Biosciences), eluting with a buffer containing 25 mM HEPES (pH 7.8) ...
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bioRxiv - Immunology 2021Quote: ... following the BD Rhapsody System mRNA Whole Transcriptome Analysis (WTA) and Sample Tag Library Preparation Protocol (BD Biosciences). The final libraries were quantified using a Qubit Fluorometer with the Qubit dsDNA HS Kit (Q32851 ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... All expression constructs were tagged C-terminally with hexahistidine tags in the baculoviral transfer vector pAcGP67A (BD Biosciences). Proteins were purified using Ni-NTA agarose (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... antibody mix 3 (Supplementary Table S4) with Sample Tag oligonucleotide-conjugated antibodies (Single-Cell Multiplexing Kit, BD Biosciences) was added to the cell suspension and left on ice for 15 minutes for staining ...
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bioRxiv - Immunology 2024Quote: ... following the BD Rhapsody System mRNA Whole Transcriptome Analysis (WTA) and Sample Tag Library Preparation Protocol (BD Biosciences). The final libraries were quantified using a Qubit Fluorometer with the Qubit dsDNA HS Kit (Q32851 Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... BCL-2 (Clone#7/BCL-2, BD Bioscience), MCL-1 (Cat#600-401-394 ...
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bioRxiv - Developmental Biology 2019Quote: ... Interactions with desalted analytes diluted in PBS at 100 nM (recombinant CBP domains 6His-tag Full HAT, Bromodomain (BD), RING) ...
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bioRxiv - Systems Biology 2021Quote: ... BD Sample Tags were size-separated by SPRI selection after cDNA amplification and amplified according to standard protocols (BD User-Demonstrated Protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then placed the male in an ice-slurry for 10 seconds as anaesthesia before photographing him to measure his standard length (SL) and body depth (BD). Photographs were analysed using ImageJ [61] ...
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bioRxiv - Immunology 2021Quote: ... 250.000 PBMCs per sample (two patients and two healthy controls) were incubated with an individual oligo-labelled antibody (Multiplex Tag, BD Human Single-Cell Multiplexing Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... and MDAMB231-NS cells separately with the Sample-Tags from the BD Human Immune Single-Cell Multiplexing Kit (BD Biosciences) as described in the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... 8 color (4-2-2) configuration (BD Bioscience, Heidelberg, Germany). The system automatically adjusts spillover values for the compensation of standard fluorochromes ...