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Citations for Becton, Dickinson and Company :
301 - 350 of 758 citations for Round Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and then tetramer+CD8+7-AAD- cells were sorted into 96-well plates (NIPPON Genetics, Cat# 4ti-0770/C) by using an FACS Aria II (BD Biosciences). TCRα and TCRβ cDNA pairs were amplified from single T cells by a one-step multiplex RT-PCR method described in our previous study19 ...
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bioRxiv - Microbiology 2020Quote: ... Parasites expressing the transfected Cas9-GFP were sorted by flow cytometry into 96 well plates containing HFF monolayers using a BD Influx cell sorter (BD Biosciences). This line was used for complementing pSFP1myc constructs ...
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bioRxiv - Molecular Biology 2020Quote: ... the average of the fluorescent intensity was calculated as the proteasome activity as follows: 30 µl of each fraction sample was transferred to a 96-well BD Falcon microtiter plate (BD Biosciences), and mixed with 2 mM ATP and 0.1 mM fluorogenic peptide substrate Suc-Leu-Leu-Val-Tyr-AMC (Peptide Institute) ...
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bioRxiv - Developmental Biology 2019Quote: ... were sorted directly into four 96 well plates « Precise WTA Single Cell Encoding Plate » (BDTM Precise WTA Single Cell Kit, BD Genomics) (1 cell/well ...
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bioRxiv - Immunology 2019Quote: ... were resuspended in D-PBS supplemented with 2% (v/v) FBS containing the antibody cocktail in 96-well microtiter plates (BD Biosciences) for 20 min on ice in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... GFP+ cells were sorted in 96-well plates 48 h post transfection using a FACS Aria III cell sorter (BD BioSciences). Single cells were grown until formation of viable individual clone in DMEM medium supplemented with 50% fetal bovine serum and 1% penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2020Quote: ... Single nuclei with low Hoechst/PI fluorescence (G1 population) were sorted into 96-well plates containing freezing buffer using a FACSJazz (BD Biosciences). Pre-amplification-free single-cell whole genome sequencing libraries were prepared using a Bravo Automated Liquid Handling Platform (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... and monoclonal cell lines were generated by isolating cells expressing both TauRD fusion proteins in a 96 well-plate by cell sorting with a BD FACS Aria III (BD Biosciences) following amplification ...
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bioRxiv - Cancer Biology 2021Quote: ... Transduced cells were selected with 5μg/ml blasticidin and bulk cells expressing the TetR protein were clonally isolated in 96-well plates using FACS (BD-Aria). BRAFV600E and BRAFWT were expressed in cells using Gateway-compatible lentiviral constructs (pLenti CMV/TO puro DEST ...
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bioRxiv - Microbiology 2021Quote: ... Second-passage cells were grown to confluency in 24- or 96-well tissue culture plates (Costar, Van Nuys, CA) on fibronectin (BD Biosciences). All incubations were in 5% CO2 at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... and GFP-positive single cells were sorted by FACS into 96-well plates using a FACSAria III cell sorter (BD Biosciences) to obtain single cell-derived clones ...
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bioRxiv - Genomics 2022Quote: ... Forty-eight hours after transfection single GFP+ cells were sorted into 96-wells plates on FACS ARIA II/III Flow Cytometer (BD Biosciences) and expanded ...
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bioRxiv - Cell Biology 2023Quote: ... Jurkat cells were cultured for 3 days after electroporation and sorted into 96-well plates for clonal expansion using fluorescent signal to isolate edited clones using the BD Bioscience Influx sorter (BD Bioscience). Both alleles of AP-2 and one allele of DNM2 were tagged in the final cell line.
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bioRxiv - Systems Biology 2023Quote: ... all transformants were cultured individually in 96-well plates in 1 ml of the appropriate minimal liquid media (AD: -Leu; BD: -Trp) at 30°C until saturated (∼36-48 hours) ...
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bioRxiv - Genetics 2023Quote: ... For each reporter cell line single cells were FACS-sorted into 96-well plates using a FACSAria III cell sorter (BD Bioscience) to obtain single-cell derived clones ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were dissociated using Trypsin-EDTA and BFP-positive cells were individually seeded into wells of a 96-well plate for single-cell culture by fluorescence-activated cell sorting (FACS) using BD FACSAria III (BD Biosciences). As described by Zhang et al ...
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bioRxiv - Biophysics 2023Quote: ... Single cell clones of Cas9 and sgKRAS-transduced cells were sorted into 96-well plates using a FACSMelody benchtop sorter (BD Biosciences) and verified for successful knock-out of KRas by Western blot analysis and PCR amplicon sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... 48h post transfection cells were sorted for single cell clones by seeding one RFP- and GFP-positive cell per 96-well using a FACS Melody sorter (BD Bioscience). The deletion of IGF2BP1 was validated by western blotting ...
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bioRxiv - Genomics 2022Quote: ... single cells expressing GFP (expressed from the CRISPR plasmid) were sorted into 96-well plates through flow cytometry using a BD FACSAria Fusion (BD Biosciences). Single-cell clones were cultured and tested for successful knockout of RFX7.
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bioRxiv - Microbiology 2023Quote: ... GFP-positive cells were sorted and plated in a 96-well plate for single-clone selection with Becton Dickinson FACS Aria Fusion (BD Science). SAMHD1 deficiency was tested by Interferon-α stimulation and subsequent western blotting for SAMHD1 ...
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bioRxiv - Bioengineering 2024Quote: ... cells were analyzed and mCherry positive clones were FACS sorted as single cells in 96-well plates using ARIAIII (BD Biosciences). Successfully expanded clones were then further characterized.
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bioRxiv - Biophysics 2024Quote: ... single cells were gated to include low sfGFP signal and sorted into 96-well plates on BD FACSAria Fusion (BD Biosciences) at the Flow Cytometry Resource Facility at Princeton University ...
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bioRxiv - Microbiology 2021Quote: ... the ELISA was performed by coating 96 well plates with 2 µg/mL LRRC15-His (AcroBio, #LR5-H52H3) in coating buffer (BD, #51-2713KC) overnight at 4°C ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Lentivirus was titrated by serial dilution of lentivirus and incubating with 5000 HEK293T cells in 96 well plates with 8 µg/ml polybrene for 3 days followed by GFP detection by flow cytometry (BD Biosciences LSRFortessa). Transduction units (TU ...
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bioRxiv - Molecular Biology 2020Quote: ... A tube formation assay was performed by seeding HAEC in 96-well plate coated with Matrigel with reduced growth factors (BD Biosciences, CA) in the presence of human VEGF (10-20 ng/mL ...
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bioRxiv - Genetics 2020Quote: Both live cells and fixed cells were sorted into 96 well plates containing cell lysis buffer using BD Aria™ IIIu sorter (BD Biosciences). FSC parameters were used for singlet selection ...
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bioRxiv - Biophysics 2022Quote: ... serial dilutions of overnight cultures were performed in 96-well plates and then spotted with a micropipet or pin replicator (“frogger”) on plates containing LB agar (BD Difco #244510) and 2% arabinose (CHEM-IPEX #01654 ...
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bioRxiv - Cell Biology 2023Quote: ... NIH/3T3 cells were transfected with pSpCas9(BB)-2A-GFP:sgRNA-RhoB and single GFP-positive cells sorted into 96-well plates via FACS using Aria-II SORP (BD Biosciences, Heidelberg, Germany). Cells were expanded and single clones screened for the absence of RhoB expression via immunoblotting ...
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bioRxiv - Cell Biology 2023Quote: ... or 48 hrs (MDA-MB-231) prior to sorting mNeon-green positive cells into 96 well plates using the FACSMelody™ (BD Biosciences). Single cell clones were expanded and screened by PCR for homozygous knock-in of the DFN cassette.
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bioRxiv - Immunology 2022Quote: ... 20,000 BMDMs were plated in a 96 well plate with 50,000 purified naive OT-I T cells labeled with Violet Proliferation Dye 450 (BD Biosciences, Franklin Lakes, NJ). Following 72 hours of incubation at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... the BECs were seeded in 96-well plates coated with collagen type I (5 µg/mL, #354231, BD Bioscience, San Jose, CA, USA) at a density of 104 cells per well ...
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bioRxiv - Neuroscience 2022Quote: ... Samples are ultracentrifuged and pellets were resuspended and incubated onto 96 well ELISA plates containing 5ug/mL anti-syn42 monoclonal antibody (BD transduction #610-787). ELISAs were carried out on test samples run alongside serial diluted standard synthetic α-Syn ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells from this population were then singled-out in 96-well plates in conditioned medium containing 20% FBS (using a BD FACSAriaTM cell sorter) and individual clones were allowed to expand before genomic DNA was again extracted ...
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bioRxiv - Synthetic Biology 2023Quote: ... The exconjugants were picked with Pickolo colonypicker and inoculated into 96 deep-well plates containing 1.5 mL of TSB medium (Tryptic soy broth, BD 211825, Thermo Fisher Scientific) supplemented with apramycin (50 μg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... the first stimulus was presented (Bottom Dot, BD). After disappearance of the first fixation point ...
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bioRxiv - Cancer Biology 2019Quote: ... After selection of BFP positive cells using one round of FACS (BD FACS Aria II), cells were transduced with lentiviral particles produced using vector pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (Addgene #60904 ...
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bioRxiv - Cancer Biology 2020Quote: ... single cells were isolated and sorted into a 96 well plate using an Influx Special Order fluorescence activated cell sorter (BD Biosciences, San Jose, CA) at the Stanford Shared FACS Facility ...
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bioRxiv - Neuroscience 2021Quote: ... FACS was then used to separate single RFP-positive cells in a 96-well plate using the 561 nm laser on a FACSAriaIII (BD Biosciences, San Jose, CA). Indels at the corresponding target sites in the clones were analyzed by genomic PCR ...
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bioRxiv - Immunology 2022Quote: ... and F15 NOD Nfκb1 heterozygote were suspended in biotin-free RPMI containing 0.1% azide and 3% FCS and surface stained in 96-well plates with the 10–3.6 PE (anti-I-Ag7) (BD PharMingen, Franklin Lakes, NJ, USA), which is class II major histocompatibility complex (MHC ...
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bioRxiv - Cell Biology 2022Quote: ... Cells grown on 96 well plates were fixed and permeabilized for 20 mins at 4°C with Fixation/Permeabilization solution (BD Biosciences, cat. no. 554714). Cells were washed 3 times in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... cells were single-sorted into 96-well plates containing complete media + ISRIB using a BD Biosciences FACSAria Fusion (BD Biosciences, San Jose, CA, USA). Clones were expanded and genomic DNA was sequenced to confirm the desired mutations ...
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bioRxiv - Cancer Biology 2024Quote: ... GFP-positive cells were selected and seeded individually in 96-well plates by flow cytometry (BD FACSAriaTM III, BD Biosciences, Franklin Lakes, NJ, USA). Double SNX1 and SNX2 KO cells were obtained using SNX2 KO cells ...
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bioRxiv - Immunology 2023Quote: ... 5.0 × 104 CD4+ T cells were serially diluted onto either α-IL-2 or α-IFNɣ sterile coated PVDF 96 well plates (BD Biosciences, NJ, USA). The CD4+ T cells were then activated for 24 or 72 h in complete DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... The final pellet containing organoids was re-suspended in Matrigel and distributed in 96-well culture plates containing an underlay Matrigel layer (Growth Factor Reduced Matrigel, BD Biosciences, San Jose, CA, USA). Cultures were maintained at 37 °C in serum-free basal medium (DMEF/F12 ...
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bioRxiv - Microbiology 2023Quote: ... CLASP11256−1538- and CD2AP-TurboID constructs were sorted into a 96-well plate as single cells with the FACS sorter Aria III (BD Biosciences, San Jose, California, USA).
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bioRxiv - Cancer Biology 2024Quote: ... single GFP-expressing cells were sorted in 96-well plates by a fluorescence-activated cell sorter (FACS) FACSAria II cell sorter (BD Biosciences, San Jose, CA, USA). Two weeks later ...
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bioRxiv - Genomics 2020Quote: ... prior to a second overnight round of primary antibody binding: rat anti-CD31 (557355, BD Biosciences) or rat anti-CD8a (14-0808-82 ...
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bioRxiv - Developmental Biology 2020Quote: ... the enrichment of pachytene spermatocytes and round spermatids was performed on a FACSAria III cell sorter from BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... sorting for top 25% expression vs bottom 50% expression (BD Aria). Sorted cells were then unfixed overnight in 50 μg/mL proteinase K (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... placed in the bottom of a 10 mL syringe (BD 302995). 2 mL washed Fractogel EMD SO3-(M ...