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Citations for Becton, Dickinson and Company :
451 - 500 of 736 citations for Recombinant Red Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccinebioRxiv - Microbiology 2020Quote: ... protein transport inhibitors (BD GolgiPlug™, BD Biosciences) were added and incubated with cells for 6 hours ...
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bioRxiv - Microbiology 2020Quote: ... protein Transport Inhibitor (BD Golgiplug, BD Bio-Sciences) was added to the wells ...
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bioRxiv - Microbiology 2020Quote: ... protein Transport Inhibitor (BD Golgiplug, BD Bio-Sciences) was added to the wells ...
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bioRxiv - Immunology 2021Quote: ... GolgiStop and GolgiPlug Protein Transport Inhibitors (BD Biosciences) were added to the cell cultures ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated proteins were tetramerized with streptavidin-PE (BD) at a 4:1 molar ratio.
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bioRxiv - Immunology 2024Quote: ... along with protein transport inhibitors Golgiplug (BD Biosciences) (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... stained with a collection of different fluorescent labelled antibodies (CD4-BV785; BioLegend, San Diego, CA, USA; CD25-PE-Cy5.5; BD Bioscience; and CD54-AF700; EXBIO) and analysed on a Cytoflex flow cytometer (Software CytExpert 2.3 ...
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bioRxiv - Microbiology 2020Quote: ... or 0 to 10−2 fluorescent intensity (AF647-negative) were captured and segregated in 2 separate 5 ml round-bottom tubes (BD Biosciences, San Jose, CA) containing 1% BSA/PBS ...
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bioRxiv - Immunology 2021Quote: ... Appropriate compensation and fluorescence-minus-one (FMO) controls were generated in parallel either with aliquots of bone marrow cells or Rainbow Fluorescent Particle beads (BD Biosciences; Cat. No. 556291). All antibody concentrations were titrated prior to injury study using primary bone marrow cells to ensure optimal separation and detection of true negative and positive populations ...
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bioRxiv - Cancer Biology 2020Quote: Cells were treated as indicated and stained with fluorescent conjugates of annexin-V and PI (1 µg/ml final concentration) and analyzed on a FACSCanto machine (BD, Franklin Lakes, NJ, USA). Annexin V was prepared as previously described (Brumatti et al. ...
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bioRxiv - Microbiology 2019Quote: ... following which they were trypsinized and assessed for GFP expression via fluorescent activated cell sorting using the Accuri C6 (BD Biosciences, San Jose, CA). Analysis was performed using FlowJo Macintosh v10.1 (FlowJo ...
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bioRxiv - Physiology 2020Quote: ... Appropriate compensation and fluorescence-minus-one (FMO) controls were generated in parallel either with aliquots of bone marrow cells or Rainbow Fluorescent Particle beads (BD Biosciences; Cat. No. 556291). All antibody concentrations were titrated prior to injury study using primary bone marrow cells to ensure optimal separation and detection of true negative and positive populations ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1) after Fc blocking and analyzed using flow cytometry (BD FACS Celesta/Novocyte Advanteon). Cells were fixed and permeabilized using intracellular staining reagents (Intracellular Fixation & Permeabilization Buffer Set ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
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bioRxiv - Neuroscience 2024Quote: ... mCherry+ IPSCs were then manually isolated and expanded over several cycles to increase the percentage of mCherry+ IPSCs in culture before finite isolation by fluorescent cell activated sorting (FACS) using Influx cell sorter (BD Biosciences, San Diego, CA) with subsequent plating and expansion ...
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bioRxiv - Cancer Biology 2020Quote: ... Acridine Orange staining was measured by FACS analysis using a 488nm laser with 530/30 (GREEN) and 695/40 (RED) filters on a FACS LSR-II (BD Biosciences, Switzerland). Data were analyzed with FlowJo software (Ashland ...
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bioRxiv - Immunology 2021Quote: ... After filtration through a 200-gauge steel mesh and the removal of red blood cells with RBC lysis buffer (BD Biosciences, USA), splenocytes were collected for further experimentation ...
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bioRxiv - Cancer Biology 2021Quote: H59-GFP cells were stained with CellTracker Red according to manufacturer’s instructions and analyzed by flow cytometry (FACScan, BD Biosciences, Mississauga, ON).
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bioRxiv - Neuroscience 2020Quote: ... centrifuged 500 x g for 5 min before red blood cells were lysed in 1X PharmLyse (BD Biosciences, San Jose, CA USA) for 8 min at room temperature (RT) ...
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bioRxiv - Immunology 2022Quote: ... single-cell suspensions were subjected to osmotic red blood cell lysis and incubated with a combination of antibodies: CD4-BV421 (BD Biosciences #562891), CD8-PeCy7 (BD Biosciences #561097) ...
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bioRxiv - Cell Biology 2023Quote: ... and analysed on the green FL1-A and red FL2-A channels using an Accuri™ C6 Plus flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... Bone marrow from each mouse was then combined and subjected to red blood cell lysis in 1X BD PharmLyse (BD Biosciences, 555899). Cells were then washed with PBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and red fluorescence with a 633 nm laser (PE-H) at 510 volts on an Aria III cell sorter (BD Biosciences, USA). We used the following fluorescence gates for sort-seq (Figure S2).
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bioRxiv - Immunology 2022Quote: ... and the cell suspensions purity was higher than 90% as assessed by fluorescent staining with PhycoErytherin (PE)-anti-human CD14 antibody (BD Biosciences, San Diego, CA, USA) using a Floid Cell Imaging Station (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... The fluorescent signal from GLI1 OE and control cell samples stained for cTnT-positivity was normalized to fluorescent signal from cells stained with a BV421-conjugated isotype control IgG antibody (BD Biosciences 562438, Lot 8242926, X40). Fluorescence was quantified ...
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bioRxiv - Immunology 2020Quote: ... Single cells were stained with the different fluorescent antibodies (as indicated in the figure legends) and DsRed on the BD LSR II flow cytometer (BD Biosciences, San Jose, CA, USA). Analyses were completed using FlowJo analysis software (FlowJo ...
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bioRxiv - Genomics 2022Quote: ... Emerging cells were induced for 24 h with 2.5 µg/ml doxycycline and then subjected to Fluorescent-Activated Cell Sorting (FACS) to select the ones expressing the mVenus (BD FACSAria™ II, BD Biosciences). H3 and H4 overexpression was verified by Western Blot with anti-H3 and anti-H4 antibodies (Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 ml of cell suspension was incubated with 2 μl of Nile red solution (0.25 mg ml−1) for 5 minutes before the analysis with a bench flow cytometer (BD Accuri C6, BD Biosciences). To determine Nile red fluorescence ...
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bioRxiv - Cancer Biology 2021Quote: ... Images were captured using Olympus Cell-F software taking a dual image of brightfield and Texas Red filters and further analysed by flow cytometry (BD FACSCaliburTM, BD Biosciences). Day 0 samples (stained and unstained ...
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bioRxiv - Microbiology 2022Quote: ... Mice recovered at 32°C (Warm Air System, Safety Cabinet Version red, VetTech, UK) until spontaneous movement and received 0.8mg/kg subcutaneous (BD Microlance 27G 13mm needle) atipamezole to reverse the ɑ-agonist into the neck scruff.
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bioRxiv - Cell Biology 2021Quote: ... miR mimics and antagomirs were mixed with Matrigel (Matrigel Basement membrane matrix, growth factor-reduced, Phenol Red-Free, ldEV-Free, Cat # FAL356231, BD, Franklin Lakes, NJ). The ear lobes of mice were stretched over the middle finger and a needle (1 ml insulin syringe ...
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bioRxiv - Genomics 2022Quote: ... 1ml diluted blood was transferred into a clean 15ml-tube and incubated with 9ml 1x red blood cells lysing solution (BD Pharm Lyse, 555899) at room temperature for 15min avoiding light ...
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bioRxiv - Immunology 2022Quote: ... cells were detached using TrypLE™ Express Enzyme (1X), no phenol red, and stained with anti-CD31 (APC, Anti-Mouse) (BD Pharmingen™) MHCI (A488 ...
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bioRxiv - Molecular Biology 2019Quote: ... 1:5000 and HRP Protein A (BD Biosciences, 610438) 1:5000 ...
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bioRxiv - Immunology 2021Quote: ... Golgi Plug protein transport inhibitor (BD Biosciences, cat 555029) was added at a 1/1000 dilution ...
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bioRxiv - Immunology 2022Quote: ... 0.27ul of the GolgiStop protein transport inhibitor (BD #554724) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... Specific proteins were detected using anti-TRIM25 (BD biosciences), anti-NS1 (Santa Cruz Biotechnology) ...
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bioRxiv - Immunology 2022Quote: ... BD GolgiStop™ protein transport inhibitor (BD Bioscience, 554724), and antibody for CD107a (BD Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... or mouse antibodies recognizing cellular proteins (anti-EEA1, BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... GolgiPlugTM Protein Transport Inhibitor (1:1000, BD Biosciences, BD555029) was added after 3h co-culture ...
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bioRxiv - Immunology 2023Quote: Antibodies against the following proteins were purchased from BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2) cytosolic protein markers: anti-TSG101 (mouse, BD Biosciences ...
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bioRxiv - Systems Biology 2023Quote: ... or a different type of measurement for each entry (for instance, protein levels rather than UMI counts in the BD Rhapsody protein data).
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bioRxiv - Neuroscience 2023Quote: ... A 40 μM cell strainer was used for aggregates removal after dissociation and TdTm-expressing RGCs were isolated by fluorescent-activated cell sorting (BD FACS Aria™ III. BD Bioscience). Total RNA extraction was performed with Arcturus Picopure RNA isolation Kit (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were trypsinized and median MitoTracker™ Green FM and MitoSOX™ Red fluorescence was measured on a FACSCanto™ II cytometer (BD Biosciences) illuminating with Ar 488 nm laser and excluding cell debris and doublets from the analysis ...
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bioRxiv - Cell Biology 2022Quote: ... Homogenate was passed through 40-μm nylon filter to obtain a single-cell suspension and subjected to red blood cell lysis reagent (BD Pharm Lyse, BD Biosciences). Cells were subjected to negative selection using CD45 magnetic microbeads (Miltenyi Biotec # 130-052-301) ...
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bioRxiv - Molecular Biology 2021Quote: ... Stained peripheral blood samples were then lysed with red blood cell lysis buffer and absolute cell counts calculated using BD Liquid Counting Beads (BD Biosciences, San Jose, CA). Flow cytometry was performed using BD Fortessa II instrument (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescence was measured at a fixed emission (670 nm) after excitation with a red laser (640 nm) using a BD FACSVerse Flow cytometer (BD Biosciences, San Jose, CA) with 10,000 events per sample ...
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bioRxiv - Immunology 2021Quote: ... Spleen cells were isolated by scraping with slides in sterile PBS following red blood cell (RBC) lysis (Pharm LyseTM; BD Bioscience, San Jose, CA). Extracted LN were derived from the cervical and axillary chains exclusively.
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bioRxiv - Genetics 2023Quote: ... were isolated by 1X Red Blood Cell lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...