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Citations for Becton, Dickinson and Company :
651 - 700 of 4271 citations for Recombinant Mouse Cd99 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and non-specific binding was blocked by adding 5% (v/v) normal donkey serum and Fc block (BD). Avidin was used to neutralize endogenous biotin ...
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bioRxiv - Neuroscience 2021Quote: ... The remaining myelin-depleted cell suspension was blocked for ten minutes with Fc preblock (CD16/ CD32, BD 553141) on ice and stained for 20 minutes with antibodies to distinguish brain endothelial cells (CD31+/ CD45-) ...
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bioRxiv - Cancer Biology 2020Quote: ... myeloid and hematopoietic stem and progenitor antibody panels after blocking with anti human fc block (BD Pharmingen 564219) for 10 minutes at room temperature (Supplementary Table 7 ...
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bioRxiv - Cancer Biology 2021Quote: ... The purification efficacy was analyzed by FC using the following surface markers: anti-CD45/PE antibody (BD Bioscience), anti-CD56/BB515 antibody (BD Bioscience ...
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bioRxiv - Microbiology 2022Quote: ... and Fixable Viability Dye eFluor 780 (Thermo Fischer) in presence of FC Block (BD, 20 min, 4°C). Stained cells were washed in FACS buffer twice and fixed in 2% paraformaldehyde (30 min ...
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bioRxiv - Immunology 2019Quote: ... Following FACS sorting the individual blood and tissue-derived subsets were incubated with Fc block reagent (BD Biosciences) and Sample Tag antibodies for 20 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Without additional washing, an antibody cocktail containing Fc block (14-0161-86, eBioscience) BV buffer (566349, BD Bioscience) were added ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples were blocked for 10 minutes on ice with 1:100 diluted FC Block (BD Pharmingen, Clone 2.4G2) and metal-conjugated antibody cocktail added and incubated for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Surface antibody staining was done in PBS containing 1% FCS and 1X Brilliant Stain Buffer (BD Biosciences, USA) for 20 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were stained for 45 min on ice in FC buffer containing unlabelled CD32 (BD Biosciences, Sydney, Australia) to block Fc receptor binding and HIS48-FitC ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated with Fc block and surface stained with anti-CD4 BUV805 (BD Biosciences; clone: RM4-5) or CD4 APC-Alexa Fluor 780 (eBioscience ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 15 min at 4°C with Fc-block (anti-CD16/CD32, 2.4G2, BD biosciences) before staining ...
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bioRxiv - Cell Biology 2021Quote: ... and then FITC-conjugated anti-human Fc secondary antibody was used as a detection antibody (BD Biosciences, USA). Samples were analyzed by Guava® easyCyte™ Flow Cytometer (Millipore ...
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bioRxiv - Immunology 2022Quote: ... and were resuspended in FC stain buffer prior to data collection via an LSR Fortessa flow cytometer (BD). Analysis was done using FlowJo software (version 10.7.1) ...
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bioRxiv - Immunology 2022Quote: For analysis of various thymus stromal cell populations human samples were blocked with human Fc-block (BD Bioscience) for 10 minutes at 4°C and then stained with Lineage cocktail-FITC ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were sorted into HBSS-2% FCS in 15ml Falcon tubes using a BD Aria III (BD Biosciences). Monocytes were gated as Lineage-negative (B220 ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained for 45 min on ice in FC buffer containing unlabelled CD32 (BD Biosciences, Sydney, Australia) to block Fc receptor binding ...
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bioRxiv - Microbiology 2022Quote: ... Single cell suspensions stained in PBS + 2% FBS in the presence of Fc receptor blocking antibody (BD Pharmingen) and stained with the antibodies against the following mouse proteins ...
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bioRxiv - Immunology 2023Quote: ... Aspecific epitope binding was blocked with 0.5 mg/mL human Fc Block (BD Biosciences, San Jose, CA, USA). Mononuclear cells were stained for extracellular epitopes for 1h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... and Fixable Viability Dye eFluor 780 (Thermo Fischer) in presence of FC Block (BD, 20 min, 4°C). Stained cells were washed in FACS buffer twice and fixed in 2% paraformaldehyde (30 min ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with FACS buffer (PBS + 0.1% BSA) and incubated with Human Fc block (5µl per 2×106 cells; BD) at room temperature for 10 min ...
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bioRxiv - Immunology 2023Quote: Cells were harvested and suspended in 50 μL of FCM buffer (0.5% BSA and 0.05% sodium azide in PBS) with 2.5-10 μg of Human BD Fc Block (BD). Cells were stained using the antibodies listed in Supplementary Table 1 for 15 minutes in the dark at room temperature and washed three times with FCM buffer ...
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bioRxiv - Immunology 2023Quote: ... and incubated for another 15 minutes at 4°C in the presence of Fc receptor block (BD Biosciences). Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... Fc receptors were blocked with anti-CD16/32 in the antibody staining solutions (BD Biosciences, Franklin Lakes, NJ). For RPE and endothelial identification ...
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bioRxiv - Cell Biology 2023Quote: FC analyses of SkM single cell suspensions were performed with the cell analyser LSRFortessa X-20 (BD Bioscience) using the FACSDiva 8.0 software ...
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bioRxiv - Genetics 2023Quote: ... 15 mins) and resuspended in 3mL DPBS supplemented with 30 µL Human BD Fc Block (BD Pharmingen, 564220). The sample was gently rocked at room temperature for 30 mins ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/ml human recombinant epidermal growth factor (hr-EGF; 354052, BD Pharmigen, San Jose, CA, USA), 0.4 mg/ml hydrocortisone (Sigma) ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-mouse β-Catenin (clone 14/ β -Catenin, BD Biosciences); rabbit anti-mouse AKT (9272 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-mouse GS15 primary antibody (610960, BD Transduction Laboratories, UK), rabbit anti-mouse C1q primary antibody (ab71940 ...
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bioRxiv - Microbiology 2022Quote: ... AF647-conjugated mouse anti-mouse CD64 (X54-5/7.1; BD Biosciences) and AF700-conjugated mouse anti-mouse CD45.2 (104 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-NR2B (1:200, Clone 13, Mouse 610416, BD Biosciences), mouse monoclonal anti-N2B (1:200 ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines created with fluorescently tagged proteins were sorted and normalized for expression using the UC Berkeley Flow Cytometry Facility (BD Bioscience Influx Sorter). Cell lines were confirmed with confocal imaging and immunoblot.
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bioRxiv - Bioengineering 2023Quote: ... Cells were then blocked with anti-mouse CD16/32 and labeled with cell surface antibody cocktail including anti-mouse CD3-FITC、anti-mouse CD4-APC and anti-mouse CD8-Percp-cy5.5 (BD Bioscience) for 30min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Single cells from digested xenograft tumors were labeled using a BUV395 anti-mouse cocktail (pooled and diluted 1:40: anti-mouse H2kD [BD Biosciences, cat. 742437], anti-mouse CD45 [BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Single cells from digested xenograft tumors were labeled using a BUV395 anti-mouse cocktail (pooled and diluted 1:40: anti-mouse H2kD [BD Biosciences, cat. 742437], anti-mouse CD45 [BD Biosciences, cat. 567451], anti-mouse CD31 [BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies against CD16/32 (Fc block; clone 2.4G2) and ITGB2 (clone CTB104; Alexa Fluor 488 conjugated) were from BD Pharmingen and Santa Cruz Biotechnology Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... for 15 minutes to block Fc receptors and then stained using with antibodies to CD45-APC-Cy7 (BD 557659) and SYTOX Red Dead Cell Stain (Life Technologies S34859 ...
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bioRxiv - Immunology 2020Quote: ... cells were washed in perm/wash solution and resuspended in 0.5% PFA-FC stain buffer prior to running on a Symphony A5 flow cytometer (BD). Analysis was performed using FlowJo software ...
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bioRxiv - Immunology 2019Quote: ... FCS in PBS and were stained for 1 hour at 37°C with Vγ5 TCR-FITC (clone 536; BD), MHCII-AF647 (I-A/IE ...
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bioRxiv - Immunology 2020Quote: ... and 10% (v/v) FCS (from herein referred to as DC culture media. The antibodies were purchased from BD, Miltenyi ...
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bioRxiv - Cancer Biology 2021Quote: ... dissociated tumor cells were Fc receptor blocked by incubating 1*106 cells/50-100 µl of staining buffer with 2.5 µg of Human Fc Block (Cat # 564220; BD) for 10 minutes.
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bioRxiv - Neuroscience 2023Quote: ... Fc receptors were blocked for 15 min with rat anti-CD16/32 (1:100, catalog no. 553141; BD Biosciences) and cells were washed and labeled with the following antibodies for 30 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed and blocked with blocking buffer plus Fc receptor blocking antibody (Clone Fc1.3216, BD Biosciences, United Kingdom). Cells were incubated at RT for 10 min then centrifuged (800 g ...
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bioRxiv - Cell Biology 2023Quote: ... Data collection was done using BD FACSDiva™ software and .fcs files were formally analyzed with FlowJo (FlowJo, BD). All flow cytometry experiments contained single color controls for compensation and gating.
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bioRxiv - Neuroscience 2023Quote: ... Fc receptors were blocked for 15 min with rat anti-CD16/32 (1:200, catalog no. 553141; BD Biosciences) and cells were washed and labeled with the following antibodies for 30 min at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... All data were converted to FCS 3.0 using NF Profession (Version 1.0, NanoFCM) and analyzed with FlowJo (Version 10.8.1, BD, Franklin Lakes, NJ).
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bioRxiv - Immunology 2023Quote: ... Non-specific antibody binding was blocked by incubating the cells with Fc-blocking antibody 2.4G2 (BD, 25 μg/ml) for 10 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed in PBS+2% FCS and permeabilised for 10 minutes in 70ul of Cytofix/Cytoperm (BD Biosciences). After washing in 2mL of PermWash ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in FACS buffer and incubated in FC Block for 5 minutes (1:60, BD Pharmingen, 553142) and primary antibodies for 10 minutes ...
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bioRxiv - Pathology 2023Quote: ... single cells were sorted into an eppendorf containing PBS with 5% FCS using the FACSAria III sorter (BD Biosciences).