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Citations for Becton, Dickinson and Company :
401 - 450 of 1822 citations for Recombinant Human S100 Calcium Binding Protein A9 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 4°C for 10 min and binding was analyzed by flow cytometer FACS LSRFortessaTM (BD Bioscience, Franklin Lakes, NJ) using Yellow/Green Ex 561 nm Em 586/15 laser for the detection of antibodies ...
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bioRxiv - Immunology 2023Quote: ... Non-specific antibody binding was blocked by incubating the cells with Fc-blocking antibody 2.4G2 (BD, 25 μg/ml) for 10 minutes on ice ...
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bioRxiv - Microbiology 2023Quote: ... staining was performed on 1×105 cells in 100 μL of 1X Annexin V binding buffer (BD Biosciences, 556454) following manufacturer recommended guidelines ...
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bioRxiv - Immunology 2023Quote: ... and blocked unspecific bindings with using anti-mouse CD16/CD32 (Mouse BD Fc Block, BD Biosciences, Franklin Lakes, NJ). Cells were then stained with FITC-conjugated anti-mouse CD45 (BioLegend) ...
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bioRxiv - Microbiology 2021Quote: ... PE-conjugated mouse-anti-human CD8 and APC-conjugated mouse-anti-human CD3 Abs (BD Pharmingen, San Jose, CA), diluted in PBS to a final volume of 50μl/well ...
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bioRxiv - Immunology 2019Quote: ... For human B cell blots a 1:250 dilution mouse anti-human HIF-1α (BD Biosciences, San Diego, CA) or for mouse blots a 1:100 dilution goat anti-mouse HIF-1α wsa added and the blots incubated O/N at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... and allophycocyanin (APC) mouse anti-human human leukocyte antigen – DR isotype (HLA-DR) (BD Biosciences; San Jose, CA, USA). Unstained cells ...
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bioRxiv - Immunology 2022Quote: ... Cell subsets for the populations from different donors were labelled simultaneously with the cell surface sorting panel (Table S4) an oligonucleotide-tagged antibody sample tag (BD Biosciences) prior to cell sorting on a FACSAria3 (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... left 24 hours before addition of drug and cell death was assessed following live cell staining with FITC-tagged AnnexinV (BD Biosciences) and addition of PI (Sigma ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were then tagged with propidium iodide and purified using flow-assisted cell sorting (FACS; BD FACS Aria II, 70 uM nozzle, BD Biosciences) and brought to a concentration of 1000 nuclei/uL ...
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bioRxiv - Microbiology 2022Quote: In vitro CYP inhibition assay was performed using a standard commercial recombinant CYPs kit (BD-Gentest) as recommended by the company ...
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bioRxiv - Immunology 2019Quote: ... Human PBMC derived T cells were stained with anti-human CD3 antibody and were acquired with FACS Calibur (BD Biosciences). Data were analyzed using CELL QUEST PRO software (BD Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse Anti-human KDR (KDR-PE, FAB357P, R&D) and Mouse Anti-Human NRP-1 (NRP-1-PE, Material Number: 565951, BD) antibodies were used at a ratio of 1:20 ...
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bioRxiv - Immunology 2019Quote: ... Primary human B cells: Human peripheral blood was collected by routine phlebotomy in heparinized vacutainers (BD Biosciences, San Diego, CA) and diluted 1:2 with PBS containing 2% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were resuspended in 200 μL 1x binding buffer with or without 5 μL Annexin V (BD Biosciences, Billerica, MA). After 15 min incubation ...
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bioRxiv - Plant Biology 2020Quote: 10-fold serial dilutions (OD600=1, 10-1, 10-2, 10-3) of yeast sample harbouring a DNA-binding domain (BD)-AtCIPK16 and either an activation domain (AD)-AtCBLs or an empty vector (AD ...
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bioRxiv - Plant Biology 2020Quote: ... 10 μL of 10-fold serial dilutions of yeast harbouring an activation domain (AD)-AtCBLs or empty vector pTOOL28 and either DNA-binding domain (BD)-AtCIPK16 or empty vector pTOOL27 were spotted on plates containing DDO (SD/-Leu/-Trp ...
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bioRxiv - Developmental Biology 2021Quote: ... and another one bearing amino acids 329–542 were cloned into the pOAD and or pOBD2 vectors (Cagney et al., 2000) in-frame either with the activator domain (AD) or the DNA-binding domain (BD) sequence of GAL4 ...
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bioRxiv - Pathology 2019Quote: ... and V2C84AC86A were cloned into the activation domain (AD)-containing vector and SlSGS3 was cloned into the vector harboring the DNA binding domain (BD). Both constructs were transformed into Saccharomyces cerevisiae strain AH109 ...
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bioRxiv - Immunology 2019Quote: ... were labeled with a cocktail of non-APC binding antibodies namely the murine T-cell lineage directed anti-Thy-1.2 antibody (BD Pharmingen) and the T-cell subsets recognizing CD4 antibody (eBioscience ...
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bioRxiv - Biochemistry 2021Quote: ... The expression and binding signals were determined by flow cytometry using BD Accuri™ C6 Flow Cytometer (BD Biosciences, USA). The cell analysis and sorting were done by S3e Cell Sorter (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... coding sequence were inserted into pGADC1 vector harbouring GAL4 activation domain (AD) and pBDUC1 vector harbouring GAL4 DNA binding domain (BD).
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bioRxiv - Microbiology 2020Quote: ... washed twice with cold PBS and then re-suspended in 1x binding buffer(Apoptosis detection Kit I # 556578, BD Pharmingen) at 1x 106 cells/ml ...
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bioRxiv - Neuroscience 2020Quote: The vector used to insert the bait cDNAs in the yeast two-hybrid (YTH) screens was Gal4 DNA binding domain (Gal4-BD) expression vector pAS2-1 (Clontech ...
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bioRxiv - Microbiology 2020Quote: ... Gene sequences without predicted introns were inserted into the vectors pGAD24 and pGBKT7 generating translational fusions to the Gal4 activation domain (AD) and DNA-binding domain (BD), respectively.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in 200 uL of binding buffer after discarding the supernatant and analyzed via flow cytometry using a FACSCalibur Flow Cytometer (BD).
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bioRxiv - Molecular Biology 2020Quote: ... CDS (pENTR/D-TOPO) were sub-cloned into both pGADT7-GW (activation domain, AD) and pGBKT7-GW (binding domain, BD) to generate SWCDS-AD and SWCDS-BD ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in 200 uL of binding buffer after discarding the supernatant and analyzed via flow cytometry using a FACSCalibur Flow Cytometer (BD).
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bioRxiv - Cancer Biology 2019Quote: ... About 1×104 cells were collected and analysed for binding efficacy of the chimeras using FACS (BD FACS Calibur system).
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bioRxiv - Cancer Biology 2019Quote: ... and resuspended in Annexin V binding buffer (10 mM HEPES 7.4, 140 mM NaCl, 2.5 mM CaCl2) with Annexin-V FITC (BD Biosciences 556419). Flow cytometry was performed on a Becton Dickinson LSRFortessa or LSRII flow cytometer ...
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bioRxiv - Microbiology 2020Quote: ... cells were resuspended and 2-43 binding to cell-surface S trimer was quantified on LSRII flow cytometer (BD Biosciences). The mean fluorescence intensity of APC in GFP-positive cells was analyzed using FlowJo and the relative binding of 2-43 to S trimer in the presence of competitors was calculated as the percentage of the mean fluorescence intensity compared to that of the competitor-free controls.
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bioRxiv - Microbiology 2021Quote: ... from pDONR207 into the yeast two-hybrid vector pPC97-GW for expression in fusion downstream of the GAL4 DNA-binding domain (GAL4-BD). The control siRNA and a pool of TAX1BP1 siRNA (Ambion ...
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bioRxiv - Genetics 2020Quote: ... Both WT and TG8B RTY were transferred to pACT-GW (GAL4-Activation Domain, AD) and pAS-GW (GAL4-Binding Domain, BD) (Nakagawa et al. ...
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bioRxiv - Cell Biology 2022Quote: ... was then transferred to a 96-well plate and fALOD4-Neon binding was measured by analyzing 10,000 RBCs for each condition on a Stratedigm S1000 EX Flow Cytometer (BD Biosciences). Fluorescently labeled RBCs were analyzed using FlowJo software version 10 (Ashland ...
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bioRxiv - Plant Biology 2022Quote: ... while the full-length coding sequence of HlMYB7 was cloned into the prey vector pGADT7-GW [DNA-binding domain (BD)] (Addgene Inc ...
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bioRxiv - Plant Biology 2022Quote: ... and the PCR products were fused into the activation domain (AD) vector pGADT7 and/or the DNA-binding domain (BD) vector pGBKT7 and verified by sequencing ...
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bioRxiv - Immunology 2022Quote: ... the amplified PGRP3 cDNA was digested with BamHI and EcoRI and ligated to a pGBKT7 vector containing the GAL4 DNA-binding domain (BD). Successful recombinant pGBKT7-PGRP3 bait construction was verified with restriction digestion (BamHI ...
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bioRxiv - Cell Biology 2023Quote: ... of BicDR were cloned into pOAD and pOBD2 vectors so that they were in frame with the activator domain (AD) or the DNA binding domain (BD)13,30 ...
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bioRxiv - Plant Biology 2023Quote: ... Obtained fragment was cloned into pDONR207 via Gateway BP reaction with following LR reactions into pGBKT7-GW (binding domain, BD) or pACT2-GW (activation domain ...
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bioRxiv - Immunology 2023Quote: ... or showed a complete loss of antibody binding (AF647-/PE+) were isolated using FACS (BD Aria Fusion or Influx BD). Collected cells were pelleted by centrifugation at 3500 x g for 3 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The binding of IGSF3-Fc to the cell surface was detected as the APC fluorescence intensity using FACSVerse (BD Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... Full-length or truncation (ΔTM) of TbPEX15 with and without mutations were cloned in the pPC97 vector containing GAL4- DNA Binding Domain (BD) as described in Table 1 ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed once with binding buffer and resuspended in PBS prior to detection with an Accuri C6 Plus flow cytometer (BD). Data were exported to Prism (GraphPad ...
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bioRxiv - Immunology 2023Quote: ... high-binding 96-well plates were coated with 5 μg/ml anti-CD3 (Clone UCHT1, BD Pharmingen™ #555329, USA) in PBS (ThermoFisher Gibco™ #21600044 ...
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bioRxiv - Cell Biology 2024Quote: ... mESC pellets were incubated with FITC conjugated Annexin V and Propidium Iodide in 1X binding buffer as per manufacturer’s protocol (BD Pharmingen). 10,000 events were acquired per sample ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated for 15 minutes at room temperature in the dark before adding 400μl of Annexin Binding Buffer and proceeding with the acquisition using a LSR Fortessa Cell Analyzer (BD Biosciences) or a Symphony A1 cytometer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... APC Mouse Anti-Human CD8 (Cat555369; BD Pharmingen), PE-CF594 Mouse Anti-Human CD68 (Cat564944 ...
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bioRxiv - Cancer Biology 2021Quote: ... PE Mouse Anti-Human CD4 (Cat555347; BD Pharmingen), APC Mouse Anti-Human CD8 (Cat555369 ...
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bioRxiv - Cancer Biology 2021Quote: ... BV421 Mouse Anti-Human CD27 (Cat562514; BD Horizon).