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Citations for Becton, Dickinson and Company :
51 - 100 of 2239 citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Epigenetic alterations underlie airway macrophage differentiation and phenotype during lung fibrosisbioRxiv - Immunology 2020Quote: ... BAL cells (1 × 107) were incubated with human Fc-block (BD Biosciences, USA) and human CD206 APC-Cy7 (clone 15-2 ...
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bioRxiv - Microbiology 2022Quote: Cells were blocked with Human BD Fc Block (Fc1.3216, 1:100, BD Biosciences) in the presence of Live/Dead Fixable Aqua (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... 3 x 106 BM cells were incubated with human Fc BlockTM (BD, 564219) for 10 minutes at RT ...
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bioRxiv - Immunology 2020Quote: ... cell suspensions were spin and resuspended in HBSS containing human Fc-Block (BD Biosciences). Cells were incubated with V450-labeled mouse anti-human FcgRI (CD64) ...
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bioRxiv - Immunology 2020Quote: Immune cells were treated with human- and mouse-specific Fc Block™ (BD Biosciences) at room temperature for 10 minutes to prevent non-specific antibody staining ...
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bioRxiv - Cell Biology 2023Quote: ... blocked with 1% BSA and human BD Fc block (BD Bioscience, Franklin Lakes, NJ) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... 1-2 x 106 BM cells were incubated with human Fc BlockTM (BD, 564219) for 10 minutes at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with 0.05 µg/µl of Human Fc Block (BD Biosciences #564219) for 20 minutes prior to antibody staining with anti-GLUT1 (Abcam #EPR3915 ...
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bioRxiv - Cancer Biology 2020Quote: Meiotic and kinetochore genes tagged with Enhanced Green Fluorescent Protein (EGFP) (from BD Biosciences) were used ...
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bioRxiv - Neuroscience 2020Quote: ... Human Soluble Protein buffer kit (BD Biosciences) was used in conjunction with flex sets for detecting granulocyte-macrophage colony-stimulating factor (GM-CSF) ...
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bioRxiv - Immunology 2022Quote: To analyze the major thymic stromal cell types in human samples single cells suspensions non-specific antibody binding was blocked with human Fc-Block (BD Bioscience) and the cells were subsequently stained with CD45-PE ...
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bioRxiv - Cancer Biology 2022Quote: ... Reconstituted mouse bone marrow and human AML was blocked with mouse Fc block (BD Biosciences) and human IgG (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were incubated with 1% Human Fc Block (clone Fc1.3216, BD Biosciences, San Jose, CA) on ice for 10min ...
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bioRxiv - Bioengineering 2024Quote: ... and resuspended in 0.25 ug of Human Fc Block (BD Biosciences, Franklin Lakes, NJ, USA) diluted in staining buffer to block non-specific antibody binding ...
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bioRxiv - Immunology 2022Quote: Cells for B cell staining panels were resuspended in 50uL Human Fc-Block (BD Cat#564220) diluted to1:500 in PBS + 1%FBS and incubated for 30 minutes at 4°C prior to washing and surface staining ...
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bioRxiv - Immunology 2023Quote: ... cells were spun down and resuspended in 50μL of human Fc block (BD Biosciences Cat. 564220) in DPBS and incubated for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and blocked for 15 minutes with 1:20 human FC block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE-Cy7 anti-human CD71 (TFRC ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated in a blocking solution containing Human BD Fc Block (564220; BD Biosciences) diluted 1:25 (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... samples were blocked with Human BD Fc Block™ solution (1 µL/200,000 cells) (BD Biosciences) for 10 minutes at room temperature to reduce any non-specific antibody staining by Fc Receptors expressed on macrophages ...
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bioRxiv - Cell Biology 2019Quote: ... Cells containing Atto488-tagged protein (530 nm) were detected by flow cytometry on a FACSCalibur (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... CR3022 IgGs and recombinant hACE2-Fc at their respective EC80 concentrations through flow cytometric analysis on the BD FACS Canto II (BD Biosciences). Binding signal was normalized and calculated as a percentage of the binding signal to reference WT RBD-SD1 ...
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bioRxiv - Immunology 2021Quote: ... cells were spun down and resuspended in 50 μL of human Fc block (BD Biosciences Cat. 564220) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Immunology 2022Quote: ... the cells were first incubated for 10 min with human Fc block (#564220, BD Biosciences, CA, USA) in DPBS-10% FCS ...
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bioRxiv - Immunology 2023Quote: ... Cells at a concentration of 5 million/ml were first incubated with human Fc block (BD Biosciences) on ice for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/ml human recombinant epidermal growth factor (hr-EGF; 354052, BD Pharmigen, San Jose, CA, USA), 0.4 mg/ml hydrocortisone (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... myeloid and hematopoietic stem and progenitor antibody panels after blocking with anti human fc block (BD Pharmingen 564219) for 10 minutes at room temperature (Supplementary Table 7 ...
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bioRxiv - Cell Biology 2021Quote: ... and then FITC-conjugated anti-human Fc secondary antibody was used as a detection antibody (BD Biosciences, USA). Samples were analyzed by Guava® easyCyte™ Flow Cytometer (Millipore ...
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bioRxiv - Immunology 2023Quote: ... Aspecific epitope binding was blocked with 0.5 mg/mL human Fc Block (BD Biosciences, San Jose, CA, USA). Mononuclear cells were stained for extracellular epitopes for 1h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with FACS buffer (PBS + 0.1% BSA) and incubated with Human Fc block (5µl per 2×106 cells; BD) at room temperature for 10 min ...
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bioRxiv - Genetics 2023Quote: ... 15 mins) and resuspended in 3mL DPBS supplemented with 30 µL Human BD Fc Block (BD Pharmingen, 564220). The sample was gently rocked at room temperature for 30 mins ...
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bioRxiv - Immunology 2023Quote: Cells were harvested and suspended in 50 μL of FCM buffer (0.5% BSA and 0.05% sodium azide in PBS) with 2.5-10 μg of Human BD Fc Block (BD). Cells were stained using the antibodies listed in Supplementary Table 1 for 15 minutes in the dark at room temperature and washed three times with FCM buffer ...
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bioRxiv - Cancer Biology 2020Quote: FITC-tagged AnnexinV (BD-Biosciences), PI(Sigma ...
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bioRxiv - Immunology 2019Quote: ... The cells were resuspended in FACS buffer (HBSS/ 1% FBS) then treated with Fc block (anti-mouse clone 2.4G2 and anti-human clone 3G8) purchased from BD and stained with specific antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... dissociated tumor cells were Fc receptor blocked by incubating 1*106 cells/50-100 µl of staining buffer with 2.5 µg of Human Fc Block (Cat # 564220; BD) for 10 minutes.
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bioRxiv - Cell Biology 2019Quote: Cells expressing eGFP-tagged huntingtin proteins were harvested and eGFP expression was analyzed on an LSR Fortessa (BD Pharmingen) flow cytometer for PulSA analysis to detect protein aggregates ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... cells expressing TagRFP-tagged proteins were flow-sorted using a BD Influx cell sorter (BD Bioscience, San Jose, CA). TagRFP emission was triggered by 561 nm laser ...
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bioRxiv - Immunology 2023Quote: ... Fluorescent antibody or recombinant protein biding on fluorescent nanobeads were directly counted and measured by BD FACSCelesta™ flow cytometer.
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bioRxiv - Bioengineering 2019Quote: ... the monocytes were incubated for 10 min at room temperature with human BD Fc-block (2.5 µg per 1×106 cells in 100 µL, Cat. no. 564220, BD biosciences). The cells were further incubated with the following antibody cocktail for 30 min at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... live/dead stain in PBS for 15 min on ice before washing and re-suspending in 2x human/mouse Fc block in stain buffer (BD) followed by surface-staining with monomeric GFP and anti-human CD45 AF700 (Biolegend clone HI30) ...
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bioRxiv - Immunology 2024Quote: Enriched B cells were resuspended in ice cold FACs buffer at a density of 106 cells/100 μL and treated with Human Fc Block (BD) according to manufacturer instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... cells were washed again and re-suspended in permeabilization buffer with the following fluorescently-tagged antibodies targeting intracellular T-lymphocyte proteins for 30 min: IL-4 BV 421 (BD Biosciences clone 11B11 ...
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bioRxiv - Biophysics 2020Quote: ... Positive cells were amplified under antibiotic selection pressure and sorted for low or intermediate expression level of the fluorescently tagged protein on an Aria II Fluorescence Activated Cell Sorter (BD). Each sorted population was then used for pilot experiments to determine the lowest possible expression level required for optimal imaging conditions ...
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bioRxiv - Immunology 2022Quote: ... 2% Fc block (BD), and fixable viability dye eFluor 450 (ebiosciences) ...
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bioRxiv - Immunology 2021Quote: ... Fc block (BD Biosciences) was added to each sample at a 1:100 dilution with decoy tetramer (Bio-PyS23 ...
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bioRxiv - Immunology 2021Quote: ... and Fc Block (BD) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... and Fc Block (BD) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and Fc Block (BD) for 10 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and Fc block (BD) on ice for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: Dissociated and resuspended iTF-Microglia were blocked for 15 min with 1:20 Human FC Block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE/Cy7 anti-human CD184 (CXCR4 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then washed three times in PBS followed by incubation in 1% Human Fc Block (BD Biosciences, San Jose, CA) in PBS ...