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Citations for Becton, Dickinson and Company :
151 - 200 of 2396 citations for Recombinant Human EPCAM Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... and antibody to Fc receptor binding inhibitor (eBioscience) before incubation with the following primary antibodies: PE-conjugated anti-human CD31 (BD Pharmingen), PE-conjugated anti-human CD45 (BD Pharmingen) ...
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bioRxiv - Immunology 2020Quote: ... samples were first pre-incubated with human BD Fc Block to block non-specific binding (BD Pharmingen, Franklin Lakes, NJ, USA) for 20 minutes at room temperature ...
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bioRxiv - Genomics 2022Quote: ... 106 cells were resuspended in PBS supplemented with 2% fetal bovine serum and incubated with human Fc blocking solution (BD Biosciences) for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was discarded and the pellet was resuspended in Staining buffer containing the Human BD Fc Block™ antibody (BD, 564220) and incubated with agitation for 15 min at 4°C in a cold room ...
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bioRxiv - Immunology 2023Quote: ... purified NK cells were incubated for 10 minutes at room temperature with 2.5µg of Human BD Fc Block followed by addition of 10μg/mL of anti-NKG2D purified antibody (BD Biosciences, clone 1D11) or isotype control antibodies prior to addition of SVG-A cells ...
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bioRxiv - Bioengineering 2023Quote: ... At the end of the co-culture the cells were washed twice and incubated with human or mouse Fc receptor blocking antibodies (BD Biosciences). Human macrophages were stained with anti-CD11b (M1/70 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then harvested and incubated in a blocking buffer containing 10%FBS and 1μg/mL Human Fc block (BD Biosciences, USA) for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 1.5% FCS) containing 1 μl Fc block anti-CD16/CD32 (2.4G2, BD Biosciences) for 20 min ...
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bioRxiv - Immunology 2021Quote: ... Cells were preincubated with Fc Block (Mouse BD Fc Block™; BD Biosciences) for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were preincubated with Fc Block (Mouse BD Fc Block™; BD Biosciences) for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... and Fc receptors were blocked using FC Block (1:100) (BD biosciences # 553141). Cells were then stained in 96-well round-bottom plates ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Developmental Biology 2021Quote: ... epithelial enrichment was performed on some samples by incubation with Epcam antibody (BD cat # 563477). In the same manner ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used for immunofluorescence: α-EpCAM-BV421 (clone EBA-1) from BD Biosciences ...
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bioRxiv - Developmental Biology 2019Quote: ... The EpCAM+CD45-CD31- cells were then sorted via flow cytometry (Aria II, BD Biosciences). The isolated cells were centrifuged at 300g for 10 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... and stained for Epcam using an anti-CD326 antibody (BD Biosciences, # 563214, 1/500 dilution) and the live/dead stain DRAQ7 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... and 5e6 spleen cells were restimulated in vitro with 5e6 irradiated ERAAP KO spleen cells and 20 U/ml of recombinant human IL-2 (BD Biosciences). After 6 days ...
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bioRxiv - Immunology 2022Quote: ... Spike and RBD tetramers were prepared fresh before use and formed by combining biotinylated proteins with PE-conjugated Streptavidin (BD Biosciences) at a molar ratio of 4:1.
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in 50 µL of FACS buffer (PBS plus 1% BSA) and blocked with µL 1 human Fc block (BD Biosciences, 564219) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Macaque cells were incubated with 100 μl of FACS buffer (PBS 1x with 2% fetal bovine serum and 1mM EDTA) with human Fc Block (BD Biosciences #564219) at a 1:500 dilution for 30 min on ice.
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bioRxiv - Microbiology 2022Quote: ... Fc receptors were blocked with anti-CD16/32 Fc block antibody (BD Biosciences, 553142) and Rat IgG then stained with primary antibodies ...
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bioRxiv - Immunology 2023Quote: ... Fc blockade was performed prior to extracellular staining with Mouse BD Fc Block (BD). Cell surface antigens were stained for 30 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... and biotinylated anti-mouse IFN-γ (BD) was added ...
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bioRxiv - Neuroscience 2021Quote: ... After Fc blocking (BD Biosciences), cells were incubated with viability probe (FVS780 ...
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bioRxiv - Cancer Biology 2022Quote: ... Fc Block (BD Biosciences 553142) was added at 5 μL/well and plate was incubated for 5 min at RT in the dark ...
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bioRxiv - Immunology 2022Quote: ... FC block (Cat# 553141, BD) was added at a1:100 dilution to prevent non-specific binding ...
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bioRxiv - Pathology 2023Quote: FCS files outputted by BD FACSCanto™ II flow cytometers were loaded into Python scripts using the FlowKit package15 and normalized to marker mean values of 0 and standard deviation values of 1 ...
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bioRxiv - Cell Biology 2020Quote: ... Epithelial cells were also excluded using anti-CD326/Epcam (BV421; Cat#563214; BD; used 1:200), and finally ...
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bioRxiv - Genomics 2020Quote: ... Cells from patients 2 through 5 were stained following washing with anti-EPCAM-PE (347198, BD Biosciences;1:50 dilution in ice-cold phosphate-buffered saline ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then stained for Epcam using an anti CD326 antibody (BD Biosciences, # 563214, 1/500) and the live/dead stain DRAQ7 (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells from patients 2 through 16 (P2-P16) were stained with anti-EPCAM-PE (347198, BD Biosciences;1:50 dilution in ice-cold phosphate-buffered saline ...
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bioRxiv - Immunology 2023Quote: ... The Fc receptors of immune cells were blocked with CD16/CD32 Fc block (BD Bioscience) in FACS buffer at 4°C for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... FCS files were exported and analyzed with FCS Express 7 and FlowJo v10.6.2 (BD Biosciences). Debris ...
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bioRxiv - Cancer Biology 2023Quote: ... 200 μL per 1x10e6 cells) with human IgG-Fc block (1:100) followed by the incubation with streptavidin-magnetic bead particles (BD Biosciences, Ref#557812) at 50 μL per 1x10e6 cells ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines created with fluorescently tagged proteins were sorted and normalized for expression using the UC Berkeley Flow Cytometry Facility (BD Bioscience Influx Sorter). Cell lines were confirmed with confocal imaging and immunoblot.
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bioRxiv - Immunology 2023Quote: ... and recombinant IFNγ (BD Pharmingen) as a standard ...
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bioRxiv - Immunology 2022Quote: ... biotinylated anti-mouse CD11c (clone HL3, BD Biosciences), biotinylated anti-mouse Ly-6G/Ly-6C (clone RB6-8C5 ...
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bioRxiv - Bioengineering 2019Quote: ... and biotinylated anti-mouse CD184 (20.2%; BD Pharmingen). The cells were washed in 2% FBS in HBSS through 5 min centrifugation (300g ...
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bioRxiv - Genomics 2020Quote: ... and biotinylated goat anti-rat (559286, BD biosciences) all 1:100 in TNB with 10% pre-immune goat serum were allowed to bind for 1 h ...
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bioRxiv - Immunology 2024Quote: ... biotinylated anti-IgG1 (clone A85-1, BD Biosciences) was added at 1 µg/mL in 10% FBS in PBS ...
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bioRxiv - Cell Biology 2020Quote: Equal number of cells were then blocked with 2µl FC-Block (Mouse BD Fc Block™) per 100µl cell suspension for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: Equal number of cells were then blocked with 2µl FC-Block (Mouse BD Fc Block™) and 2.5µl rat serum (10710C ...
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bioRxiv - Immunology 2022Quote: ... Fc receptors of cells were blocked using CD16/CD32 antibodies (Mouse BD Fc Block; BD Biosciences) for 15 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Fc receptors of cells were blocked using CD16/CD32 antibodies (Mouse BD Fc Block; BD Biosciences) for 15 min at 4 °C ...
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bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Immunology 2021Quote: ... Primary epithelial cells (singlets, alive, CD45−, EpCAM+ cells) were isolated with a FACSAria III cell sorter (BD) with a cell purity typically around 96% ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... anti-EpCAM-PE and anti-Podoplanin-APC and sorted using a FACSAria III instrument (BD Biosciences, Germany). From these donors ‘Neg (mesenc)’ or ‘SMC’ fractions were not collected ...
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bioRxiv - Cancer Biology 2019Quote: ... flow cytometry analyses and sorting of EpCAM positive cells were performed with a FACSARIA III (BD Biosciences). Then ...
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bioRxiv - Neuroscience 2022Quote: ... and Fc block (BD Biosciences 553142) was added at 2 μL per 106 cells ...
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bioRxiv - Genomics 2019Quote: ... with mouse Fc Block (BD #553141) on ice for 15 minutes ...