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Citations for Becton, Dickinson and Company :
551 - 600 of 1965 citations for Recombinant Human CD40LG Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Samples were treated with BD Fc Block (Rat Anti-mouse CD16/CD32; BD Biosciences, USA) for 20 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... liver lymphocytes were treated with an Fc block (anti-CD16/32, clone 2.4G2; BD Biosciences) and live/dead dye for 30 min ...
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bioRxiv - Immunology 2023Quote: ... Antibody stainings were preceded by adding Fc block (anti-CD16/CD32; clone 2.4G2, BD Biosciences). For sorting ...
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bioRxiv - Cancer Biology 2024Quote: ... FCS files were analyzed using FlowJoTM v10.8 Software (BD Life Sciences, Franklin Lakes, NJ, USA). MAIT cells were defined from the lymphocyte as live singlet ...
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bioRxiv - Immunology 2021Quote: ... or for 4 h with 20 ng/ml recombinant mouse IL-4 (BD Pharmigen). Cells were then washed twice with isotonic buffer composed of 147 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: Cells expressing eGFP-tagged huntingtin proteins were harvested and eGFP expression was analyzed on an LSR Fortessa (BD Pharmingen) flow cytometer for PulSA analysis to detect protein aggregates ...
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bioRxiv - Microbiology 2021Quote: ... creating either N- or C-terminally tagged GAL4 activation domain (AD/prey) or GAL4 DNA-binding domain (BD/bait) fusion proteins ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... cells expressing TagRFP-tagged proteins were flow-sorted using a BD Influx cell sorter (BD Bioscience, San Jose, CA). TagRFP emission was triggered by 561 nm laser ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 enriched or unenriched cells were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). From infant thymus and PBMC donors up to 5 populations were sorted after doublet ...
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bioRxiv - Microbiology 2019Quote: Human erythrocytes were harvested from 10 mL of human blood following treatment in sodium heparin tubes (BD). Whole blood was centrifuged at 500 × g for 10 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Human IFN-γ ELISA Set (555142) and Human IL-2 ELISA Set (555190) were purchased from BD OptEIA™ ...
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bioRxiv - Immunology 2021Quote: ... PerCP/Cy5.5 mouse anti-human CD95 (DX2) and Brilliant Violet 605 mouse anti-human CD28 (28.2) (BD Biosciences); Phycoerythrin (PE ...
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bioRxiv - Immunology 2022Quote: ... The Perm B solution contained PE anti-human MIP-1β and FITC anti-human IFNγ (both from BD) for intracellular cytokine staining ...
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bioRxiv - Immunology 2022Quote: ... 2 µl anti-human CD38 PE-Cy7 and 0.5µl anti-human CD3 BB700 (all antibodies sourced from BD). Surface staining incubation was performed at 4°C for 30 min ...
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bioRxiv - Immunology 2019Quote: Fc receptors were blocked with appropriate sera or rat-anti-mouse CD16/CD32 (2.4G2; BD Biosciences). All labelings were performed in PBS containing 2% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then blocked with CD16/CD32 (1:500; Fc Block™; BD Biosciences, RRID: AB_394656) for 5 min and incubated for 30 min with optimal concentrations of fluorochrome-conjugated antibodies (Table S2 ...
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bioRxiv - Bioengineering 2019Quote: ... cells were incubated with Fc block solution (purified anti-mouse CD16/CD32, clone 2.4G2, BD Biosciences) for 15 min at room temperature to prevent non-specific binding ...
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bioRxiv - Cancer Biology 2020Quote: ... RBCs were lysed and samples were subsequently incubated with anti-mouse Fc block (BD Pharmingen, 553142) for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: Single cell suspensions were incubated on ice with mouse Fc receptor block (BD Biosciences, 1:200) followed by antigen-specific antibodies in FACS buffer ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with Fc block (1:500 dilution, Cat. # 553142, clone 2.4G2, BD Biosciences) diluted in FACS buffer for 5 minutes to block nonspecific binding of antibodies to cellular Fc receptors ...
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bioRxiv - Immunology 2021Quote: ... for 10 minutes followed by an Fc block (catalog number 553142, BD Pharmingen, Franklin Lakes, NJ) at 1:1,000 for 15 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Flow Cytometry Standard (FCS) 3.0 files were analyzed with FlowJo software version X 0.7 (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... Barcoded cells were then combined into a single tube prior to Fc receptor blocking (BD Biosciences) and staining with a cocktail of metal-conjugated antibodies against cell-surface markers for 30 minutes at RT with frequent gentle shaking ...
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bioRxiv - Immunology 2020Quote: Single cell suspensions were first stained with anti-mouse CD16/CD32 (Fc-block; BD Biosciences; 553142) and dead cells were excluded with the Fixable Viability Dye eFluor506 (eBioscience ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked using α-CD16/CD32 antibody (clone 2.4G2, BD Biosciences, Bedford, MA, USA). The cells were then stained with directly labeled antibody against CD5 (APC ...
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bioRxiv - Immunology 2022Quote: Splenocyte cell suspensions (~2 million cells in HBSS) were incubated with Fc block (553142, BD Biosciences) for 10 min on ice followed by the direct addition of the following antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Final gating analysis was conducted using FCS Express software (De Novo) or FlowJo V10.8.1 (BD Biosciences). Control wells containing no chemokine in the bottom well were averaged and normalized as 100% migration ...
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bioRxiv - Immunology 2019Quote: ... Non-specific binding was blocked with rat anti-mouse CD16/CD32 Fc block (2.4G2, BD Biosciences) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Single cell suspensions of BAL and lung digests were preincubated with Fc Block antibody (BD PharMingen) in PBS + 2% heat-inactivated FBS for 10 min at room temperature before staining ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% FCS at 37 °C with either PMA/ionomycin (Leukocyte activation cocktail, BD Biosciences) for 3 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... Fc receptors were blocked with anti-FcR (anti-CD16 CD-32 at 5μg/ml, BD Pharmingen). After 2 washes in PBS 2% FCS ...
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bioRxiv - Immunology 2021Quote: ... and with Mouse BD Fc Block™ (≤ 1 μg/million cells in 100 μl, BD Biosciences) at 4°C for 10 min ...
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bioRxiv - Immunology 2021Quote: ... while in PBS and then resuspended in rat anti-mouse CD16/32 (Fc block, BD Biosciences) antibody for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Myelin-depleted cell suspensions were incubated with an anti-CD16/CD32 Fc-blocking antibody (BD Biosciences) for 30 minutes at 4 °C to avoid Fc receptor binding and then washed once with FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... cells were blocked in FACS buffer supplemented with anti-CD16/CD32 antibody (Fc Block, BD Pharmingen).
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bioRxiv - Immunology 2020Quote: Single cell suspensions were first stained with anti-mouse CD16/CD32 (Fc-block; BD Biosciences; 553142) and dead cells were excluded with the Fixable Viability Dye eFluor506 (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... and cells were blocked in FACS buffer containing Fc Block anti-CD16/32 (2.4G2) (BD Biosciences), 5% normal hamster serum ...
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bioRxiv - Immunology 2021Quote: ... Unspecific binding by the Fc receptor was prevented by incubation of anti-mouse CD16+CD32 (BD) for 20 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions were incubated on ice with mouse Fc receptor block (BD Biosciences, 1:200) followed by antigen-specific antibodies in FACS buffer ...
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bioRxiv - Immunology 2022Quote: ... resuspended in Fc Block (clone 2.4G2, Becton, Dickinson, and Company [BD, Franklin Lakes, NJ], cat. 553142) and incubated for 5 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... CD4 (#560181), CD11b (#553312), 2.4G2 (Fc block, #553142) and Perm/Wash Buffer (#554723) were from BD Pharmingen (San Diego ...
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bioRxiv - Cancer Biology 2022Quote: Immune cells were first incubated with Fc block (anti-CD16/CD32 1:100; BD Biosciences #553142) and then stained with one of two immunophenotyping panels diluted in 1x FACS buffer (2% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pre-incubated with mouse Fc blocker (BD 553142, 1 μg/million cells in 100μl) on ice for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with appropriate antibodies for 25 minutes on ice in the dark ...
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bioRxiv - Cancer Biology 2023Quote: Anti-CD16/CD32 Rat anti-mouse Fc Block (BD Pharmingen, #553142, 0.5 mg/mL, 1:400)
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bioRxiv - Immunology 2024Quote: ... Cells were stained with fluorescent dye-labeled antibodies after incubation with Fc block reagent (BD Biosciences), and analyzed on the FACSCanto Ⅱ (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: MLN cell suspensions were incubated with Fc-block (anti-CD16/CD32, BD Biosciences, cat no. 553142) followed by staining with antibodies against surface and intranuclear markers ...
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bioRxiv - Cell Biology 2020Quote: ... RPE1 cells stably expressing YFP-tagged BBSome subunits were sorted using the 488 nm laser on FACSAria IIu (BD Biosciences).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... cells were washed again and re-suspended in permeabilization buffer with the following fluorescently-tagged antibodies targeting intracellular T-lymphocyte proteins for 30 min: IL-4 BV 421 (BD Biosciences clone 11B11 ...
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bioRxiv - Biophysics 2020Quote: ... Positive cells were amplified under antibiotic selection pressure and sorted for low or intermediate expression level of the fluorescently tagged protein on an Aria II Fluorescence Activated Cell Sorter (BD). Each sorted population was then used for pilot experiments to determine the lowest possible expression level required for optimal imaging conditions ...