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51 - 100 of 501 citations for Recombinant H9N2 A Guinea fowl Hong Kong WF10 99 HA Protein His Avi tagged Biotinylated since 2019

• Plasma cell differentiation, antibody quality, and initial germinal center B cell population depend on glucose influx rate

  Shawna K. Brookens, et al., bioRxiv - Immunology 2023

  Quote: ... S1pr2-CreERT2 were purified by depleting CD90+ T cells and CD138+ cells using biotinylated anti-Thy1.2 Ab and biotinylated anti-CD138 Ab followed by streptavidin-conjugated microbeads (iMagTM; BD Biosciences, San Jose CA). B cells from CD45.1 ...
• Bone Marrow Stromal Cells in a Mouse Model of Metal Implant Osseointegration

  Alexander Vesprey, et al., bioRxiv - Cell Biology 2020

  Quote: ... resuspended in buffer solution (PBS + 2% FBS + 2mM EDTA) and blocked with recombinant Fc protein (1:100 dilution, BD Biosciences, cat#553142). Cells were then incubated with the primary antibody solution (1:100 dilution ...
• MRBLE-pep measurements reveal accurate binding affinities for B56, a PP2A regulatory subunit

  Jamin B. Hein, Martha S. Cyert, Polly M. Fordyce, bioRxiv - Bioengineering 2020

  Quote: ... we also preincubated 200 µL of 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
• Limiting the priming dose of a SARS CoV-2 vaccine improves virus-specific immunity

  Sarah Sanchez, et al., bioRxiv - Immunology 2021

  Quote: ... Kb VL8+ cells were FACS-sorted to ~99% purity on a FACS Aria cytometer (BD Biosciences) and delivered to the Northwestern University NUSeq core for scRNA-seq using Chromium NextGem 5’ v2 kit (10X Genomics) ...
• SARS coronavirus vaccines protect against different coronaviruses

  Tanushree Dangi, et al., bioRxiv - Immunology 2021

  Quote: ... Kb VL8+ cells were FACS-sorted to ∼99% purity on a FACS Aria cytometer (BD Biosciences) and delivered to the Northwestern University NU-Seq core for scTCR-seq using Chromium NextGem 5’ v2 kit (10X Genomics) ...
• Naturally enhanced neutralizing breadth to SARS-CoV-2 after one year

  Zijun Wang, et al., bioRxiv - Immunology 2021

  Quote: ... Biotinylated ovalbumin was conjugated to streptavidin-BV711 (BD biosciences, 563262) and RBD to streptavidin-PE (BD Biosciences ...
• Anti- SARS-CoV-2 Receptor Binding Domain Antibody Evolution after mRNA Vaccination

  Alice Cho, et al., bioRxiv - Immunology 2021

  Quote: ... Biotinylated ovalbumin was conjugated to streptavidin-BV711 (BD biosciences, 563262) and RBD to streptavidin-PE (BD Biosciences ...
• Conserved Neutralizing Epitopes on the N-Terminal Domain of Variant SARS-CoV-2 Spike Proteins

  Zijun Wang, et al., bioRxiv - Immunology 2022

  Quote: ... Biotinylated ovalbumin was conjugated to streptavidin-BV711 (BD biosciences, 563262), Gamma NTD to streptavidin-PE (BD Biosciences ...
• Transplantation of Muscle Stem Cell Mitochondria Rejuvenates the Bioenergetic Function of Dystrophic Muscle

  Mahir Mohiuddin, et al., bioRxiv - Cell Biology 2020

  Quote: ... and (3) biotinylated anti-mouse CD184 (1:100; BD Biosciences). After incubation for 30 min ...
• Evolution of Antibody Immunity to SARS-CoV-2

  Christian Gaebler, et al., bioRxiv - Immunology 2021

  Quote: ... Biotinylated ovalbumin was conjugated to streptavidin-BV711 (BD biosciences, 563262) and RBD to streptavidin-PE (BD Biosciences ...
• Convergent Antibody Responses to SARS-CoV-2 Infection in Convalescent Individuals

  Davide F. Robbiani, et al., bioRxiv - Immunology 2020

  Quote: ... Biotinylated Ovalbumin was conjugated to streptavidin-BV711 (BD biosciences, 563262) and RBD to streptavidin-PE (BD biosciences ...
• CCR5 deficiency impairs CD4⁺ T cell memory responses and antigenic sensitivity through increased ceramide synthesis

  Ana Martín-Leal, et al., bioRxiv - Immunology 2020

  Quote: ... -CD69-PeCy7 (H1.2F3) and biotinylated anti-CXCR5 (2G8) from BD-Biosciences ...
• Activation of Autoreactive Lymphocytes in the Lung by STING Gain-of-function Mutation Radioresistant Cells

  Kevin MingJie Gao, et al., bioRxiv - Immunology 2023

  Quote: ... A biotinylated-conjugated anti-kappa light chain antibody (BD 559750) and streptavidin-HRP (southern Biotech 1050-05 ...
• The adaptive immune response to Trichuris in wild versus laboratory mice: An established model system in context

  Iris Mair, et al., bioRxiv - Immunology 2023

  Quote: ... Parasite specific antibody was measured using biotinylated IgG1 (BD Biosciences) or IgG2c (BD Biosciences ...
• Time-resolved proximity labeling of protein networks associated with ligand-activated EGFR

  Mireia Perez Verdaguer, et al., bioRxiv - Systems Biology 2022

  Quote: Recombinant human EGF was purchased from BD Biosciences (San Jose ...
• Flotillin-2 regulates EGFR activation, degradation, and cancer growth

  David J. Wisniewski, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Recombinant human EGF was purchased from BD Biosciences ...
• Unraveling the intracellular cross-talk governing the balance between TNFα mediated survival and apoptosis signaling

  Sharmila Biswas, et al., bioRxiv - Systems Biology 2021

  Quote: ... and anti-active Caspase3- V450 tagged antibody)) were procured from BD Biosciences.
• NECAB2 participates in an endosomal pathway of mitochondrial stress response at striatal synapses

  Diones Bueno, et al., bioRxiv - Neuroscience 2023

  Quote: ... Alexa-488 tagged cytochrome c antibody (BD Biosciences, Franklin Lakes, USA), as well as the secondary antibody goat-anti-rabbit Alexa 568 (1:1000 ...
• CITE-seq analysis reveals human cytomegalovirus and diabetes-associated adaptive NK cell alterations in cardiovascular disease

  Sujit Silas Armstrong, et al., bioRxiv - Immunology 2024

  Quote: ... the cells were tagged using a Sample Multiplexing Kit from BD. Biosciences ...
• RIF1 acts in DNA repair through phosphopeptide recognition of 53BP1

  Dheva Setiaputra, et al., bioRxiv - Molecular Biology 2021

  Quote: ... using biotinylated anti-IgG1 followed by PE-conjugated streptavidin (BD Biosciences). Anti-B220 was used to confirm the purity of B cell samples.
• Post-stroke administration of the p75 neurotrophin receptor modulator, LM11A-31, attenuates chronic changes in brain metabolism, increases neurotransmitter levels, and improves recovery

  Thuy-Vi V. Nguyen, et al., bioRxiv - Neuroscience 2021

  Quote: ... rat anti-mouse B220/CD45R biotinylated (BD Biosciences, San Jose, CA), and hamster anti-CD3e (BD Biosciences) ...
• Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed myelinating oligodendrocytes in PLP1 mutant mice

  Tassnim Abdelwahab, et al., bioRxiv - Neuroscience 2023

  Quote: ... rat anti-CD8 biotinylated (1:500, catalog no. 553028; BD Biosciences), rabbit anti-LAG3 (1:100 ...
• The role of B cells in immune cell activation in polycystic ovary syndrome

  Angelo Ascani, et al., bioRxiv - Immunology 2023

  Quote: ... the biotinylated forms of anti-mouse IgG1 (BD Biosciences; A85-1) or anti-mouse IgG2b (BD Biosciences ...
• YTHDF1 drives intestinal immune response against bacterial infection

  Xin Zong, et al., bioRxiv - Immunology 2020

  Quote: ... Myc-tagged DDX60 was cloned into pRK-5 vector (BD PharMingen, 556104). We generated truncation plasmids based on full-length proteins ...
• Thymic dendritic cell-derived IL-27p28 promotes the establishment of functional bias against IFN-γ production in newly generated CD4⁺ T cells through STAT1-related epigenetic mechanisms

  Jie Zhang, et al., bioRxiv - Immunology 2024

  Quote: ... All these cells were sorted to > 99% purity with a FACS Aria II (BD Biosciences, San Diego, CA).
• In vivo single-cell CRISPR uncovers distinct TNF-α programs in clonal expansion and tumorigenesis

  Peter F. Renz, et al., bioRxiv - Cancer Biology 2023

  Quote: The sgRNA containing region has been amplified from BD Rhapsody beads in a separate nested PCR reaction ...
• DOCK2-deficiency causes defects in anti-viral T cell responses and poor control of herpes simplex virus infection

  Katrina L. Randall, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD8 (as above) and anti-CD69 (HI.2F3, BD Bioscience) or anti-IRF4 (3e4 ...
• Gata2-regulated Gfi1b expression controls endothelial programming during endothelial-to-hematopoietic transition

  Cansu Koyunlar, et al., bioRxiv - Developmental Biology 2022

  Quote: ... CD31 was visualized by using biotinylated CD31 rat anti-mouse (BD Biosciences) and Cy5-conjugated Streptavidin (Jackson Immunoresearch ...
• Lymph node expansion predicts magnitude of vaccine immune response

  Alexander J. Najibi, et al., bioRxiv - Immunology 2022

  Quote: ... Plates were washed and biotinylated anti-mouse IgG1 and IgG2a antibodies (BD Biosciences ...
• Antigen-specific humoral immune responses by CRISPR/Cas9-edited B cells

  Harald Hartweger, et al., bioRxiv - Immunology 2019

  Quote: ... Biotinylated antibodies were subsequently incubated with Streptavidin-HRP (BD Pharmingen Cat.# 554066), diluted 1:1000 in PBS ...
• Novel mRNA-based VP8* vaccines against rotavirus are highly immunogenic in rodents

  Sandro Roier, et al., bioRxiv - Immunology 2023

  Quote: ... biotinylated rat anti-mouse IgG2a antibody (1:300; BD Biosciences, Cat. 550332), or horseradish peroxidase (HRP)-conjugated rabbit anti-guinea pig IgG (H+L ...
• Human neutralizing antibodies to cold linear epitopes and to subdomain 1 of SARS-CoV-2

  Filippo Bianchini, et al., bioRxiv - Immunology 2022

  Quote: ... Biotinylated Ovalbumin and RBD were conjugated to streptavidin-BV711 (BD biosciences, 563262) and SD1-RBD to streptavidin-PE (BD biosciences ...
• Sex differences in coronary artery disease and diabetes revealed by scRNA-Seq and CITE-Seq of human CD4+ T cells

  Ryosuke Saigusa, et al., bioRxiv - Immunology 2022

  Quote: ... Cells from each subjzect were sample tagged using Sample Multiplexing Kit (BD Biosciences) which contains oligonucleotide cell labeling ...
• Transcriptome profiling reveals CD73 and age-driven changes in neutrophil responses against Streptococcus pneumoniae

  Manmeet Bhalla, et al., bioRxiv - Immunology 2021

  Quote: ... Cells were then stained for fluorophore-tagged antibodies against Ly6G (BD Bioscience # 5605991), phospho c-Jun (Ser73 ...
• Helios is a marker, not a driver, of human Treg stability

  Avery J. Lam, et al., bioRxiv - Immunology 2021

  Quote: ... recombinant human IL-12 (10 ng/ml, BD Biosciences) was added on days 5 ...
• Actin fence therapy with exogenous V12Rac1 protects against Acute Lung Injury

  Galina A. Gusarova, et al., bioRxiv - Physiology 2021

  Quote: ... Human recombinant TNFα (10-100 ng/ml, BD Biosciences), BAPTA-AM (100 μM ...
• ECM dimensionality tunes actin tension to modulate the endoplasmic reticulum and spheroid phenotype

  FuiBoon Kai, et al., bioRxiv - Cell Biology 2021

  Quote: ... Other reagents include: recombinant basement membrane (rBM; BD Biosciences), Cultrex 3D Culture Matrix Laminin I (R&D systems) ...
• PSGL-1 directs early TCR signaling to repress metabolism and promote T cell exhaustion by modulating the TCF-1/TOX axis in CD8⁺ T cells

  Jennifer L. Hope, et al., bioRxiv - Immunology 2022

  Quote: ... cells were crosslinked with biotinylated anti-hamster IgG (for anti-CD3; BD Pharmingen) and/or Goat anti-rat IgG (for anti-PSGL-1 ...
• The mycotoxin Beauvericin exhibits immunostimulatory effects on dendritic cells via activating the TLR4 signaling pathway

  Xiaoli Yang, et al., bioRxiv - Immunology 2022

  Quote: ... cells were Fc-blocked and incubated with biotinylated anti-CD4 antibodies (BD Pharmingen). Subsequently ...
• mTORC2 contributes to murine systemic autoimmunity

  Xian Zhou, et al., bioRxiv - Immunology 2022

  Quote: ... CXCR5 and PNA were stained with biotinylated anti-CXCR5 (clone: 2G8, BD Biosciences) or biotinylated peanut agglutinin (FL10-71 ...
• Type II alveolar cells with constitutive expression of MHCII and limited antigen presentation capacity contribute to improved respiratory viral disease outcomes

  Sushila A. Toulmin, et al., bioRxiv - Immunology 2021

  Quote: ... T cell IFNγ production was detected via biotinylated IFNγ detection antibody (551881, BD), followed by HRP-Streptavidin (557630 ...
• Regulatory B cells (Bregs) inhibit osteoclastogenesis and prevent bone loss in osteoporotic mice model

  Leena Sapra, et al., bioRxiv - Immunology 2021

  Quote: ... resulting cells were subjected to biotinylated mouse B cell enrichment cocktail (BD, USA) and incubated for 20-30 min at 4°C ...
• Altered hematopoietic system and self-tolerance in Bardet-Biedl Syndrome

  Oksana Tsyklauri, et al., bioRxiv - Genetics 2020

  Quote: Antibodies used for lymphocyte enrichment: biotinylated α-TCRβ (H57-597, #553169, BD Pharmingen), α-CD19 (6D5 ...
• Predictive nonlinear modeling of malignant myelopoiesis and tyrosine kinase inhibitor therapy

  Jonathan Rodriguez, et al., bioRxiv - Systems Biology 2022

  Quote: Monoclonal antibodies for flow cytometry were biotinylated mouse lineage panel (559971, BD biosciences), PE-CF594 Streptavidin (562318 ...
• Ym1 protein crystals promote type 2 immunity

  Ines Heyndrickx, et al., bioRxiv - Immunology 2023

  Quote: ... biotinylated anti-mouse IgG1 or IgM detecting antibody (0.5 mg/ml; BD Biosciences) plus streptavidin-HRP reagent (1:250 ...
• Hitting more birds with one stone: CD70 as an actionable immunotherapeutic target in recurrent glioblastoma

  M Seyfrid, et al., bioRxiv - Cancer Biology 2021

  Quote: ... CAR-T cells were stained with fluorescent tagged anti-CD3 (BD Biosciences, Cat#557851), anti-NGFR (Miltenyi Biotech ...
• Regulation of distinct STAT3 dynamics in pro (IFNγ) and anti (IL-10) inflammatory pathways and in their cross-talk: insights from a data-driven model

  U Sarma, et al., bioRxiv - Systems Biology 2019

  Quote: ... Soluble mouse recombinant IL10 and IFNγ were procured from BD Biosciences (San Diego ...
• SARS-CoV-2 Nsp14 activates NF-κB signaling and induces IL-8 upregulation

  Taiwei Li, et al., bioRxiv - Microbiology 2021

  Quote: Recombinant human TNF-α (Cat. # 554618) was purchased from BD. Biosciences ...
• CryoEM reveals unprecedented binding site for Na_V1.7 inhibitors enabling rational design of potent hybrid inhibitors

  Marc Kschonsak, et al., bioRxiv - Bioengineering 2022

  Quote: ... Recombinant baculovirus was generated using the Baculogold system (BD Biosciences) and Trichoplusia ni cells were infected for protein production ...
• Sessile alveolar macrophage connexin-43 determines mechano-immunity in the lung

  Liberty Mthunzi, et al., bioRxiv - Physiology 2023

  Quote: ... We purchased human recombinant TNFα (10 ng/ml) from BD Biosciences (San Jose ...