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Citations for Becton, Dickinson and Company :
51 - 100 of 109 citations for Recombinant Cynomolgus CD40 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... in the presence of recombinant IL2 and BD GolgiPlug (BD Biosciences) for 12 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Human recombinant growth factors PDGF and EGF were purchased from BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... PAO1 recombinant strains were selected on Pseudomonas Isolation Agar (PIA, BD) containing 100 µg ml−1 gentamicin ...
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bioRxiv - Bioengineering 2023Quote: ... Recombinant TNFα was purchased from R&D Systems (cat #: 210TA100/CF) and recombinant MCP-1 was purchased from BD Biosciences (cat # ...
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bioRxiv - Cell Biology 2019Quote: ... Cells containing Atto488-tagged protein (530 nm) were detected by flow cytometry on a FACSCalibur (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... and fluorescently tagged antibodies to label MuSCs: integrin-α7-PE (Ablab) and CD34-Alexa647 (BD Biosciences). The cell suspension was sorted by FACS to deplete non-muscle lineage cells and enrich MuSCs (Lin-/integrin-α7+/CD34+/ GFP-) ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were then tagged with propidium iodide and purified using flow-assisted cell sorting (FACS; BD FACS Aria II ...
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bioRxiv - Microbiology 2020Quote: ... Tn5 insertion recombinants were selected on Mueller Hinton (MH) agar (BD Bacto) supplemented with 7 μg.mL-1 kanamycin ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were labeled with fluorescently tagged antibodies and then were analyzed using BD LSR II (BD Bioscience) and FlowJo Software (FlowJo ...
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bioRxiv - Microbiology 2023Quote: ... Yersinia pestis strains were routinely grown in Heart Infusion (HI) broth (Difco, BD, Franklin Lakes, NJ) or on HI agar at 28°C ...
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bioRxiv - Cancer Biology 2020Quote: Antibodies utilized were directed against TFRC (PE-tagged anti-CD71 clone M-A712, BD Biosciences, San Jose, CA) and Glycophorin A (PE-tagged-anti-CD235a clone REA175 ...
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bioRxiv - Molecular Biology 2019Quote: ... the pCRKL (Y207) specific Alexa Fluor® 647 tagged antibody (cat#560790, Becton, Dickinson and Company, NJ, US) was used as per the manufacturer’s protocol in a Beckman Coulter Gallios flow cytometer (Beckman Coulter Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... All expression constructs were tagged C-terminally with hexahistidine tags in the baculoviral transfer vector pAcGP67A (BD Biosciences). Proteins were purified using Ni-NTA agarose (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... or for 4 h with 20 ng/ml recombinant mouse IL-4 (BD Pharmigen). Cells were then washed twice with isotonic buffer composed of 147 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: Cells expressing eGFP-tagged huntingtin proteins were harvested and eGFP expression was analyzed on an LSR Fortessa (BD Pharmingen) flow cytometer for PulSA analysis to detect protein aggregates ...
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bioRxiv - Microbiology 2021Quote: ... creating either N- or C-terminally tagged GAL4 activation domain (AD/prey) or GAL4 DNA-binding domain (BD/bait) fusion proteins ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... cells expressing TagRFP-tagged proteins were flow-sorted using a BD Influx cell sorter (BD Bioscience, San Jose, CA). TagRFP emission was triggered by 561 nm laser ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 enriched or unenriched cells were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). From infant thymus and PBMC donors up to 5 populations were sorted after doublet ...
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bioRxiv - Immunology 2021Quote: ... The anion exchange fractions were analyzed by Western blot using anti-His antibody (BD Pharmingen; Cat#552565) and Goat poyclonal anti-mouse IgG-HRP (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... RPE1 cells stably expressing YFP-tagged BBSome subunits were sorted using the 488 nm laser on FACSAria IIu (BD Biosciences).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... cells were washed again and re-suspended in permeabilization buffer with the following fluorescently-tagged antibodies targeting intracellular T-lymphocyte proteins for 30 min: IL-4 BV 421 (BD Biosciences clone 11B11 ...
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bioRxiv - Biophysics 2020Quote: ... Positive cells were amplified under antibiotic selection pressure and sorted for low or intermediate expression level of the fluorescently tagged protein on an Aria II Fluorescence Activated Cell Sorter (BD). Each sorted population was then used for pilot experiments to determine the lowest possible expression level required for optimal imaging conditions ...
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bioRxiv - Cell Biology 2021Quote: Expression of the various GPR116 constructs tagged with V5 or FLAG was verified by immunocytochemistry (Cytofix/Cytoperm, BD 51-2091KZ). Cells were plated in 8-chamber slides (Ibidi ...
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bioRxiv - Molecular Biology 2022Quote: ... Blood samples from HI or patients were collected in 10 ml EDTA tubes (K2E, REF 367525, BD Vacutainer) for plasma or in 4 ml CAT tubes (Clot Activator Tube ...
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bioRxiv - Biochemistry 2023Quote: ... nanobodies were purified with a 96-wells HIS-TRAP plate or a Cobalt-NTA (Talon) column (BD Biosciences), eluting with a buffer containing 25 mM HEPES (pH 7.8) ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Immunology 2022Quote: ... Cell subsets for the populations from different donors were labelled simultaneously with the cell surface sorting panel (Table S4) an oligonucleotide-tagged antibody sample tag (BD Biosciences) prior to cell sorting on a FACSAria3 (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... left 24 hours before addition of drug and cell death was assessed following live cell staining with FITC-tagged AnnexinV (BD Biosciences) and addition of PI (Sigma ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were then tagged with propidium iodide and purified using flow-assisted cell sorting (FACS; BD FACS Aria II, 70 uM nozzle, BD Biosciences) and brought to a concentration of 1000 nuclei/uL ...
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bioRxiv - Microbiology 2022Quote: In vitro CYP inhibition assay was performed using a standard commercial recombinant CYPs kit (BD-Gentest) as recommended by the company ...
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bioRxiv - Immunology 2023Quote: ... Fluorescent antibody or recombinant protein biding on fluorescent nanobeads were directly counted and measured by BD FACSCelesta™ flow cytometer.
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bioRxiv - Bioengineering 2022Quote: ... Cells were then washed with FC buffer (PBS supplemented with 2% HI-FBS and 0.05% NaN3) and resuspended in Fixation Buffer (BD Pharmingen)for 5 min at 4C° in the dark ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then placed the male in an ice-slurry for 10 seconds as anaesthesia before photographing him to measure his standard length (SL) and body depth (BD). Photographs were analysed using ImageJ [61] ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/ml human recombinant epidermal growth factor (hr-EGF; 354052, BD Pharmigen, San Jose, CA, USA), 0.4 mg/ml hydrocortisone (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines created with fluorescently tagged proteins were sorted and normalized for expression using the UC Berkeley Flow Cytometry Facility (BD Bioscience Influx Sorter). Cell lines were confirmed with confocal imaging and immunoblot.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were blocked for 10 min in ice-cold PBS+2% heat-inactivated FBS (HI-FBS) containing anti-CD16/CD32 (BD Biosciences) and rat-γ-globulin (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... Dendritic cells were cultured in the presence of 20ng/ml recombinant GM-CSF (BD Biosciences, San Jose, CA) for 7 days with a supplement of 20ng/mL GM-CSF every third day ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were activated for 72 hours in presence of recombinant mouse IL-2 (10 ng/ml) (BD Pharmingen Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... CypHer5E-stained ApoBDs were blocked with BD Pharmingen™ purified recombinant A5 in 1× A5 binding buffer (BD Biosciences ...
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bioRxiv - Systems Biology 2021Quote: ... with all differentiations performed in biological replicates. Samples were tagged or “cell hashed” (Stoeckius et al. 2018) with distinct BD sample tags (BD Bioscience, cat#PN 633780) to enable demultiplexing of cells ...
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bioRxiv - Microbiology 2021Quote: ... the ELISA was performed by coating 96 well plates with 2 µg/mL LRRC15-His (AcroBio, #LR5-H52H3) in coating buffer (BD, #51-2713KC) overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Interactions with desalted analytes diluted in PBS at 100 nM (recombinant CBP domains 6His-tag Full HAT, Bromodomain (BD), RING) ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human IgG1 Fc control and anti-human HLA class II (anti-DQ, -DR-DP) (clone Tu39, BD Biosciences) were also used at 2.5 μg/ml ...
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bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Immunology 2020Quote: ... and then labeled cells were incubated in the presence of recombinant IL-15/15Rα (50ng/mL) in the presence of absence of anti-integrin α1 (10μg/mL, BD Biosciences) or Integrin α1β1 inhibitor Obtusatin (10nM ...
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bioRxiv - Immunology 2021Quote: ... FNA samples were stained in P2 for 30 minutes on ice with biotinylated and Alexa Fluor 647 conjugated recombinant soluble Spike proteins as well as PD-1 BB515 (EH12.1, BD Horizon). Cells were then washed twice with P2 and stained with IgG BV480 (goat polyclonal ...
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bioRxiv - Microbiology 2022Quote: ... were transformed with recombinant plasmids per manufacturer instructions and grown at 37°C on selective LB agar plates (BD, 244520) and in LB broth (Fisher ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 and MIP-2 were measured using paired antibodies and recombinant cytokines according to the manufacturer’s recommendations (BD PharMingen Systems) (11).
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bioRxiv - Immunology 2020Quote: ... CR3022 IgGs and recombinant hACE2-Fc at their respective EC80 concentrations through flow cytometric analysis on the BD FACS Canto II (BD Biosciences). Binding signal was normalized and calculated as a percentage of the binding signal to reference WT RBD-SD1 ...