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Citations for Becton, Dickinson and Company :
1 - 50 of 1281 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 12 μl Bead RT/PCR Enhancer reagent from BD Biosciences and 72 μl nuclease free water for a total volume of 200 μl ...
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bioRxiv - Plant Biology 2024Quote: ... the truncated HAF2 coding regions were obtained by RT-PCR from Col-0 cDNA and then cloned into pEXPAD502 (AD) and pDBLeu (BD) backbone ...
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bioRxiv - Immunology 2024Quote: ... samples were fixed/permeabilized for 20 min at RT with the BD Cytofix/Cytoperm kit (BD Biosciences) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... for 30 min at room temperature (RT) and surface stained for 30 min at RT with anti-human antibodies for CD3 (A700, clone UCHT1, BD), CD4 (FITC,clone OKT4 ...
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bioRxiv - Immunology 2022Quote: ... fixable viability dye was included with the surface staining antibodies (20 minutes, RT) and intracellular staining used the Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... After 1h at RT with anti-RIPK1 antibody (BD Biosciences # 610459 ...
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bioRxiv - Neuroscience 2024Quote: ... RT for 3 min before staining with Annexin V (BD Pharmingen PE Annexin V apoptosis detection kit ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were analysed at RT using the FACSCanto II (BD Biosciences), first gating erythrocytes in the FSC-A/SSC scatter and then single cells in the FSC-H/FSC-A scatter ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated with Fc‐block CD16/32 (BD Biosciences; 10min, RT), followed by incubation with the extracellular antibodies (30min ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against ...
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bioRxiv - Cell Biology 2023Quote: ... washed and stained 15’ at RT with Annexin V PE (BD PharmingenTM) in annexin binding buffer ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were fixed at room temperature (RT) with Cytofix reagent (BD Biosciences) and samples were subsequently washed with BD Cytoperm solution (BD Biosciences ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were fixed for 30 minutes at RT with FACS lysis solution (BD Biosciences), followed by a final wash and analysis on a BD LSRII or FACSymphony A5 cytometer ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained 1 h at RT with primary antibodies against Salmonella (H:i, BD Difco 228241 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were stained with the following primary antibodies: Rt anti- CD31 (BD Pharmigen, 1:200), Ms anti-Aβ (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... and fixation of the iMACs for 15 min at RT in BD Cytofix buffer (BD Biosciences). Finally ...
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bioRxiv - Immunology 2024Quote: ... samples were either fixed/permeabilized at RT for 15 min with BD Cytofix reagent (BD Biosciences) or alternatively subjected to 15 min of 2% phosphate-buffered PFA prefix102 followed by subsequent overnight fixation/permeabilization at 4°C with the FoxP3 transcription buffer set ...
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bioRxiv - Molecular Biology 2020Quote: ... for 1h at RT and probed with specific primary antibodies (α-p21 (1:500, BD Biosciences, 556430), DIS3L (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 5 min at RT prior to add a 50 μl droplet of collagen type I (BD bioscience - 50:1 ratio unlabeled vs Alexa548-labeled ...
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bioRxiv - Cell Biology 2022Quote: ... Staining was performed for 20min at room temperature (RT) using CD3ε (Lin) – APC clone 145-2C11 (553066, BD), TER-119 (Lin ...
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bioRxiv - Neuroscience 2021Quote: ... slides were counterstained with DAPI (1:250 in 1X PBS, 10 mins at RT, Cat: 564907, BD Pharmingen) and mounted with Prolong™ Glass anti- fade mounting medium (Cat ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... All blood samples were clotted for 30-60 min at RT in serum separator tubes (BD, Cat# 365967), and sera were isolated by centrifugation at 4,000 x g for 15 min at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by overnight incubation at RT with combinations of primary antibodies including anti-CD31 (1:300, BD pharmingen), -HMGB1 (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... followed by secondary antibodies for 30 min in RT in Perm/Wash buffer (BD Biosciences, catalog no. 554714) supplemented with 5% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were purified with AMPure Beads (BD Bioscience ...
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bioRxiv - Immunology 2021Quote: ... and stained with antibodies directly conjugated to fluorochromes for 1hr at RT in 1X Perm/Wash buffer (BD Biosciences). NK cells were gated as viable ...
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bioRxiv - Microbiology 2022Quote: ... RT) and resuspended in 2 ml of PBS and immediately analyzed by flow cytometry (BD FACS Canto II Instrument). For each sample ...
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bioRxiv - Bioengineering 2023Quote: ... fixed with 4% PFA for 20 minutes at RT and permeabilized using Cytofix/Cytoperm (BD Biosciences, catalog no. 554714) for 20 min on ice followed by twice washes with 1x Perm/Wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... OncoImmunin Inc.) was added (5 min. at RT) and cells were acquired with a FACScan flow cytometer (BD BioSciences). The percentage of double positive eFluor+ and granzyme B+ target cells was calculated.
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bioRxiv - Developmental Biology 2023Quote: ... then incubated overnight at room temperature (RT) with combinations of primary antibodies including anti-CD31 (1:200, BD pharmingen), anti-GFP (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were blocked and incubated with the following primary antibodies for 1 hour at RT in humidity chamber (anti-CldU, Abcam ab6326, 1:100; anti-IdU, BD 347580 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated for 1 h at RT with primary antibody used as follows: mouse anti-EEA1 (BD Biosciences: 610457) 1 µg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... at 3:100 or phospho-AKT (pS473) at 1:20 dilutions for 30 mins at RT before analysis on FACSAria II (BD).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1% TritonX100 in PBS for 20 min at RT and stained overnight with rat-anti-PECAM1/CD31 antibodies (1/100, clone Mec13.3, BD Pharmingen) in blocking solution and secondary Alexa594 goat anti-rat IgG antibodies and DAPI (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... in PBS for 60 min at RT and incubation with primary antibodies (mouse anti-RAN, 1:500, BD-610340, BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by blocking with 3 % normal goat serum (NGS) in PBS for 60 min at RT and incubation with primary antibodies (mouse anti-RAN, 1:500, BD-610340 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were first blocked for non-specific antigen binding (incubating for 20 min at RT with 2 µg/ml CD16/CD32 Rat anti-Mouse, unlabeled, Clone: 2.4G2, BD, 553142) then antibodies were added without washing off the blocking solution ...
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bioRxiv - Immunology 2020Quote: ... for 5 min at RT prior to staining with a surface stain cocktail containing the following antibodies purchased from BD and Biolegend ...
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bioRxiv - Evolutionary Biology 2022Quote: The cryosections were blocked with 1% skim milk in PBS with 0.5% Tween-20 at RT for 15 min and incubated with primary antibody (rat anti-mouse anti-PECAM-1 [CD31; BD Pharmingen ...
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bioRxiv - Neuroscience 2023Quote: ... Afterwards, they were washed twice by centrifuging (500 g, 5min, RT) and resuspending in Stain Buffer (#554656, BD Pharmingen, USA). Cells were fixed in Fixation Buffer (#554655 ...
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bioRxiv - Cell Biology 2023Quote: ... pH 6.5) and specifically stained for 15 min at RT using an antibody against GPVI (HY101-PE, #565241, BD PharmingenTM) at a ratio of 1:10 ...
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bioRxiv - Cell Biology 2020Quote: ... Images were captured by a SPOT RT monochrome charge-coupled device camera (Diagnostic Instruments, Sterling Heights, MI) and processed by IPLab imaging software (BD Biosciences) and Photoshop CS3 (Adobe ...
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bioRxiv - Cell Biology 2020Quote: ... Images were captured by a SPOT RT monochrome charge-coupled device camera (Diagnostic Instruments, Sterling Heights, MI) and processed by IPLab imaging software (BD Biosciences) and Photoshop CS3 (Adobe ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with pS6 or HK2 antibody for 1 h and O-GlcNAc antibody for 20 min at RT in the dark in cytoplasmic permeabilization buffer (BD Biosciences). Nuclear permeabilization buffer was used for c-Myc staining ...
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bioRxiv - Immunology 2021Quote: ... Single cells were index sorted into 384 well plates containing 1uL of reverse transcription (RT) master mix using a FACS Aria III with FACS Diva software (BD Biosciences) to maintain protein expression with plate well location ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were stained in a solution containing 1 μg/ml PI and 4 μg/ml RNase A in PBS at 2 x 106 cells/ml for a minimum of 30 min at RT Cell cycle analysis was performed on a LSR Fortessa analyser (BD Biosciences) and analysed using FlowJo software.
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bioRxiv - Immunology 2019Quote: ... After washing in PBS and incubated for 1 hour at RT with secondary donkey-anti-rat rhodamine red 1:100 (both from BD Pharmingen) in antibody buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 4°C and secondary antibodies for 30 min in RT in Perm/Wash buffer (554714, BD Biosciences) containing 5% FBS ...
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bioRxiv - Immunology 2020Quote: ... the following antibodies and viability dye were incubated with PBMCs at RT for 20 min: anti-CD3 BUV395 (clone SP34-2; BD Biosciences), anti-CD4 BUV495 (clone SK3 ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The sections were then blocked and permeabilized with 5% Donkey serum 0.01% Triton X-100 for 30 min at RT before being incubated with rabbit anti-YAP dilution 1/100 (Signalling Technology #14074) and mouse anti beta-catenin (BD 610153) (dilution 1/200 ...