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Citations for Becton, Dickinson and Company :
201 - 250 of 266 citations for RPA reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Antibodies and reagents used for flow cytometry were: anti-Lamp1 FITC (1:200, 553793, BD Pharmingen) anti-TNFα (1:200 ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with fluorescent dye-labeled antibodies after incubation with Fc block reagent (BD Biosciences), and analyzed on the FACSCanto Ⅱ (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... samples were either fixed/permeabilized at RT for 15 min with BD Cytofix reagent (BD Biosciences) or alternatively subjected to 15 min of 2% phosphate-buffered PFA prefix102 followed by subsequent overnight fixation/permeabilization at 4°C with the FoxP3 transcription buffer set ...
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bioRxiv - Molecular Biology 2020Quote: ... Reagents for interleukin-10 analysis were: Rat Anti-Human IL-10 capture antibodies (554705, BD Biosciences, USA); Biotin Anti-Human and Viral IL-10 detection antibodies (554499 ...
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bioRxiv - Microbiology 2019Quote: ... the cells were washed in FACS buffer and fixed and permeabilized with Cytofix/Cytoperm reagent (BD Biosciences) and stained with an anti-p24 FITC antibody (clone KC57 ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 5 times with PBS-T and developed with TMB substrate reagent set (BD bioscience) with development reaction stopped with 1 M H2SO4 ...
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bioRxiv - Immunology 2023Quote: ... Then plates were incubated in the dark at room temperature with TMB substrate reagent (555214, BD Biosciences) and the colour was checked every 3 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Nonspecific binding of antibodies to cell Fc receptors was blocked using 10 μl FcR blocking reagent (BD Purified Rat Anti-Mouse CD16/CD32 553142 ...
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bioRxiv - Immunology 2023Quote: ... The cell suspensions were stained with the following anti-human antibody reagents: CD19::V500 (BD, clone HIB19), CD71::BV605 (BD ...
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bioRxiv - Immunology 2019Quote: ... The fluorescence of the detection reagent upon oxidation was analyzed by flow cytometry using a FACSCalibur (BD Biosciences).
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bioRxiv - Biochemistry 2021Quote: ... The following immunofluorescence reagents and antibodies were used: mouse anti-Paxillin (1:200, clone: 349; BD Biosciences, 612405), rabbit anti-ENAH (1:100 ...
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bioRxiv - Immunology 2022Quote: ... Stimulation was stopped by fixation and permeabilization with the BD phosphoflow staining reagents (BD cat. 558049 and 558050) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Following FACS sorting the individual blood and tissue-derived subsets were incubated with Fc block reagent (BD Biosciences) and Sample Tag antibodies for 20 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Cells were then surface stained for 30 min in the dark at 4°C with viability reagent (BD Horizon™ Fixable Viability Stain ...
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bioRxiv - Immunology 2023Quote: ... Tetramer reagent was prepared by combining equal volumes of streptavidin-PE or streptavidin-APC solutions (both from BD) at 200 ug/mL with polysaccharide-biotin solution at 20 ug/uL ...
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bioRxiv - Immunology 2023Quote: ... Sorted cells were stimulated with overlapping peptide pools of HIV consensus C and HIV-1 C.1086 Env gp140C protein (NIH AIDS Reagent Program) in R10 media in the presence of 0.2μg/mL CD28/49d co-stimulatory antibodies (BD) for 14 hours ...
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bioRxiv - Immunology 2023Quote: ... PBMCs/LN cells were stimulated with overlapping peptide pools of HIV consensus C and HIV-1 C.1086 Env gp140C protein (NIH AIDS Reagent Program) in AIM/R10 media in the presence of 0.2μg CD28/49d co-stimulatory antibodies (BD) per test ...
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bioRxiv - Microbiology 2021Quote: ... colorimetric signals were developed by addition of the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB; TMB Substrate Reagent Set, BD Biosciences) and stopped by addition of equal volume of 0.2 M H2SO4 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were permeabilized using BD Cytofix/Cytoperm and BD Perm/Wash reagents (BD Biosciences, Le Pont de Claix, France) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: The following antibodies and reagents were used for flow cytometry: CD4 clone RM-4-5 (BD Biosciences, BioLegend, Tonbo), CD8α clone53-6.7 (BD ...
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bioRxiv - Immunology 2023Quote: ... blood was sampled using a density gradient reagent for isolation of peripheral mononuclear blood cells (BD CPT Vacutainer #362753). Peripheral mononuclear blood cells (PMBCs ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed and incubated with detection antibody and enzyme reagent for 1 h at room temperature (BD Bioscience). TMB (Sigma ...
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bioRxiv - Immunology 2024Quote: ... The pooled sample was resuspended in an ice-cold sample buffer (BD Biosciences, BD Rhapsody Cartridge Reagent Kit, 633731). Isolation of single cells was performed using the single cell capture and cDNA synthesis kit according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and TNF-α (standard curve range: 0 −500 pg/mL) in swabs were determined using commercial reagents based on enzyme linked immunosorbent assay (ELISA) (BD-Biosciences ...
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bioRxiv - Immunology 2021Quote: ... From each sample 3 μL were added to a volume of 47 μL of reagents for PCR3 as described by BD’s user guide following the protocol and number of cycles listed ...
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bioRxiv - Genomics 2022Quote: ... Obtained single-cell suspensions were subjected to a BD Rhapsody system with BD Rhapsody Targeted & Abseq Reagent kit (BD Biosciences) following the manufacture’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and live/dead fixable NIR dead cell stain kit reagent according to manufacturer’s directions and analysed on a 5-laser Fortessa X20 (BD Biosciences) as previously described (Zaunders et al. ...
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bioRxiv - Cell Biology 2019Quote: IL-6 is an important cytokine implicated in inflammation and is abundantly released by tendon stromal cells isolated from patients with shoulder tendon tears after stimulation with IL-1β15 IL-6 in tissue culture supernatants was measured using enzyme-linked immunosorbent assay (ELISA) reagents (BD Biosciences) using incubations isolated from 5 donors ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... The liquid was poured off from the cells (leaving approximately 100 µl in each tube) and 5 µl of mouse Fc Blocking Reagent (rat anti-Mouse CD16/CD32 BD) was added to samples and incubated for 5 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... wells were washed 6 times as before and HRP activity was detected using TMB (3,3’, 5,5’ tetramethylbenzidine) reagent (BD OptEIA™) according to the manufacturer’s instructions (incubating for 15 −30 min) ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell whole transcriptome analysis libraries were prepared according to the manufacturer’s instructions using BD Rhapsody WTA Reagent kit (BD, 633802) and sequenced on the Illumina NextSeq 500 using High Output Kit v2.5 (150 cycles ...
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bioRxiv - Developmental Biology 2024Quote: ... Single cell whole transcriptome analysis libraries were prepared according to the manufacturer’s instructions using BD Rhapsody WTA Reagent kit (BD, 633802) and sequenced on the Illumina NextSeq 500 using High Output Kit v2.5 (150 cycles ...
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bioRxiv - Cell Biology 2022Quote: ... Homogenate was passed through 40-μm nylon filter to obtain a single-cell suspension and subjected to red blood cell lysis reagent (BD Pharm Lyse, BD Biosciences). Cells were subjected to negative selection using CD45 magnetic microbeads (Miltenyi Biotec # 130-052-301) ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were again pelleted at 500xg for 5 min at 4°C then resuspended in Cytofix/Cytoperm reagent (BD Biosciences) and incubated on ice for 30 min Following fixation/permeabilization ...
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bioRxiv - Microbiology 2020Quote: ... red blood cells in a concentration of 1 × 107 cells/mL were incubated for half an hour with 100 μl thiazole orange (TO) (Retic-Count Reagent; BD Biosciences) and Alexa-647 conjugated mouse mAb against transferrin receptor (CD71 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were fixed in Fixation/Permeabilization reagent (eBioscience) and uncompensated data was collected using a LSRFortessa cytometer and FACSDiva Software (BD Biosciences). Compensation and analyses were performed using FlowJo v10 software ...
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bioRxiv - Microbiology 2022Quote: ... Cells were subsequently fluorescently labeled for 30 minutes on ice with the following antibodies and reagents: PE-conjugated rat anti-mouse Siglec-F (E50-2440; BD Biosciences), PE-TxR-conjugated rat anti-mouse CD11b (M1/70.15 ...
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bioRxiv - Immunology 2022Quote: ... Peroxidase-like activity of heme (31) bound to STING1 was detected on the gel after incubation with TMB Substrate Reagent Set (BD Biosciences). After a blue-colored band developing ...
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bioRxiv - Neuroscience 2020Quote: ... Blood samples were diluted 1:5 with PBS and 10 µl aliquots were added to 1 ml of BD Retic-Count reagent (BD Biosciences). After a 60 min incubation in the dark at RT ...
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bioRxiv - Genomics 2019Quote: ... umbilical cord blood was collected at the time of birth in PAXgene Blood RNA Tubes with the RNA stabilization reagent (BD Biosciences) and stored at −80°C ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained with fluorochrome-conjugated antibodies or influenza HA-specific (HA518–526) H-2Kd tetramer-IYSTVASSL (MBL) reagent and analyzed using a flow cytometer (BD Biosciences). BMDCs (105 cells ...
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bioRxiv - Cell Biology 2019Quote: ... Phenotypic characterization of each lot was done by flow cytometry using the BD StemflowTM hMSC Analysis kit and the endothelial cell marker CD31 (all reagents from BD Bioscience) and showed that the 2 lots of hUC-MSC met the ISCT phenotypic criteria for MSCs (>95% positive for CD73 ...
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bioRxiv - Physiology 2021Quote: INS-1 cells were collected for flow cytometry analysis using FITC Annexin V apoptosis detection reagent (BD Biosciences, San Jose, CA). The apoptotic cells were measured by FACS Count™ Flow Cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed and then permeabilised by incubation at room temperature for 15 minutes with Fix/Perm reagent (BD Biosciences) before washing with Permwash buffer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with the medium and stained with subsequent antibodies/reagents using the Brilliant Stain Buffer Plus (BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cells were then stimulated for 24 hours in stimulation medium [CM with BD FastImmune CD28/CD49d Costimulatory Reagent (final x100, BD Biosciences) and anti-human CD40 (final concentration of 0.5μg/mL ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently labeled for 30 minutes on ice with the following antibodies and reagents: PE-conjugated rat anti-mouse Siglec-F (E50-2440; BD Biosciences), PE-TxR or PE-Cy5-conjugated rat anti-mouse CD11b (M1/70.15 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with 2% ECL prime blocking reagent in PBS-T (Amersham; #RPN418) and incubated against AP-2 (mouse-anti-AP50, BD, #A611351) and GAPDH (rabbit-anti-GAPDH ...
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bioRxiv - Biochemistry 2022Quote: ... 10 μL pre-cleared cell lysate was combined with 200 μL assay reagent containing 1 mM D-luciferin (BD Biosciences, product #556878), 3 mM adenosine triphosphate ...
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bioRxiv - Immunology 2021Quote: ... and re-suspended in 100 μL permeabilisation buffer (FACS buffer, 1% BSA, 1 mM EDTA in PBS containing 1 X BD permeabilisation reagent). FITC-conjugated anti-influenza NP monoclonal antibody (Abcam clone 20921 ...