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Citations for Becton, Dickinson and Company :
51 - 95 of 95 citations for Papain Inhibitor since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... GolgiStop solution was added (containing Monensin protein transport inhibitor) to block intracellular protein transport (BD Bioscience). As a positive control for cytokine production ...
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bioRxiv - Immunology 2021Quote: ... Protein transport inhibitor cocktail and Mouse TNF-α (560478) ELISA kit were procured from BD (USA). The following ELISA kits were brought from R&D ...
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bioRxiv - Neuroscience 2023Quote: ... protein transport inhibitors 2μl/mL GolgiPlug (Brefeldin A) and 1.3μl/mL GolgiStop (Monensin) (BD, Biosciences, USA) was added to tubes and further incubated for 3 hours at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... astrocytes (1×106) were incubated with protein transport inhibitor containing Brefeldin A (555029 Golgiplug, BD Biosciences) and Monensin (554724 Golgistop ...
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bioRxiv - Immunology 2023Quote: ... protein transport inhibitors 2μl/mL GolgiPlug (Brefeldin A) and 1.3μl/mL GolgiStop (Monensin) (BD, Biosciences, USA) were added to the tubes for 8 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... for IFN-γ assessment in T-cells in the presence of protein transport inhibitor (BD GolgiStop™) for 12 hours ...
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bioRxiv - Immunology 2023Quote: ... protein transport inhibitor was prepared according to the manufacturer’s guidelines (RRID:AB_2869014, BD Science, San Jose, CA, USA) and added to all samples ...
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bioRxiv - Cell Biology 2020Quote: ... For intracellular staining the cells were incubated for 4 h with the protein transport inhibitor GolgiPlug (BD Biosciences). For fixation and permeabilization of the cells ...
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bioRxiv - Immunology 2019Quote: ... HK Mtb or LPS (100 ng/mL) for 24h and the Golgi Plug protein transport inhibitor (BD Biosciences) was added for the last 6h according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: Cells were detached with Accutase, resuspended in differentiation media with Rock inhibitor (10µM, Miltenyi) and Draq7 (BD Bioscience), and strained (70µm ...
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bioRxiv - Immunology 2023Quote: Cells from ELISpot plate were collected in media supplemented with GolgiStop Protein Transport Inhibitor (BD Biosciences, NJ, USA) and incubated for 6 hr at 37°C ...
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bioRxiv - Immunology 2021Quote: ... rh IFN-α2a (10 ng/mL) or with the Pan Caspase Inhibitor Z-VAD-FMK (100 µM, BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: Cells were pre-stimulated with 100 ng/ml LPS together with protein transport inhibitor Golgi Plug (1:1000, BD) for 1hr at 37°C ...
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bioRxiv - Immunology 2020Quote: ... CLTX-CAR T cells were incubated with GBM cells (E:T=1:1) for 5 h in the presence of CD107a antibody and GolgistopTM protein transport inhibitor (BD Biosciences). To test for CAR T cell killing potency ...
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bioRxiv - Cell Biology 2021Quote: Internalization of oAβ-TMR in the presence and absence of inhibitors were quantified using BD FACS Aria TM (BD Biosciences) and were analysed using BD FACS DIVA TM (BD Bioscienc es ...
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bioRxiv - Immunology 2022Quote: ... Intracellular cytokine staining was performed by activating cells for 4-5h at 37°C with the Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) and subsequent fixing and staining per manufacturer’s instructions using the BD Fixation/Permeabilization Solution Kit (BD Biosciences). For intranuclear transcription factor staining ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... in the last 5 hours of culture a protein transport inhibitor Brefeldin A (1µL/mL) was added (BD Biosciences, USA). Cells were fixed using the BD Cytofix/Cytoperm® kit (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... T cells and tumor cells were co-cultured at a 1:1 effector to target (E:T) ratio for 5 hours in the presence of GolgiStop Protein Transport Inhibitor (BD Biosciences). Surface phenotype of cells was determined by flow cytometry using fluorochrome-conjugated antibodies specific for CD3 (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... 1–2 × 106 cells were incubated with 1 μM of SARS-CoV-2 minimal epitope or pool of up to ten epitopes (1 μM/peptide) for 9 h at 37°C in the presence of protein transport inhibitor (GolgiPlug; BD Biosciences ...
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bioRxiv - Genetics 2023Quote: ... Cells were stringently sorted into an Eppendorf tube containing stem cell media with 10 μM ROCK inhibitor and CloneR2 using an FACSAria III Cell Sorter (sort speed 1; BD) on the basis of eGFP and mTagBFP2 double-positivity (using the control transfection to set gating) ...
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bioRxiv - Neuroscience 2023Quote: ... and resuspended the cells in 0.3 ml of cold PBS + RNase inhibitor for sorting in a FACS Melody cell sorter (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... cells were treated for 5 hours with a protein transport inhibitor containing brefeldin A (BD Biosciences, Franklin Lakes, NJ, USA), washed and fixed for 10 minutes at 4°C with fixation/permeabilization solution (BD Cytofix/Cytoperm kit ...
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bioRxiv - Immunology 2022Quote: ... cells were stimulated for 4 h with eBioscience™ Cell Stimulation Cocktail and a protein transport inhibitor-containing Brefeldin (1.5uL/mL StopGolgi; BD Biosciences). Cells were then washed in FACS buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... in 96-well round bottom tissue culture plates in R10 media with the presence of Golgi inhibitors monensin and brefeldin A (BD Bioscience) to reach 200μL per well ...
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bioRxiv - Immunology 2022Quote: ... and 10% heat-inactivated FBS) and incubated at 37°C for 6 hours with protein transport inhibitors GolgiStop and GolgiPlug (BD Biosciences) and S glycoprotein peptide pools 1 μg/mL (JPT product PM WCPV-S-1 ...
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bioRxiv - Cancer Biology 2019Quote: ... and antibody to Fc receptor binding inhibitor (eBioscience) before incubation with the following primary antibodies: PE-conjugated anti-human CD31 (BD Pharmingen), PE-conjugated anti-human CD45 (BD Pharmingen) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then cultured for 2 hours at 37°C in 5% CO2 incubator and followed by the addition of protein secretion inhibitor cocktail containing brefeldin A and monensin (GolgiPlug, BD, 555029) for another 4-hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Confluent definitive endoderm cells are then passed 1:3 in presence of Rock Inhibitor on growth factors reduced Matrigel (BD Biosciences) and cultured for 8 days in differentiation medium (DMEM F12 ...
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bioRxiv - Cell Biology 2023Quote: ... The nuclei were washed again and resuspended in sorting buffer with 0.2 U/μl Protector Rnase inhibitor prior to cell sorting to separate the PCM1-labeled fractions using a FACS cell sorter (BD Aria). The sorted nuclei were then pelleted at 3000 × g for 10 minutes before mRNA isolation using the Direct-zol RNA Microprep kit (Zymo research R2060) ...
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bioRxiv - Neuroscience 2023Quote: ... The adherent cells that remained were either lysed with standard radioimmunoprecipitation assay (RIPA) buffer with 1mM Na3VO4 and BD Baculogold protease inhibitors (BD Biosciences) or 500uL of QIAzol® reagent (Qiagen) ...
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bioRxiv - Immunology 2024Quote: ... NK cells were either settled on substrates functionalized with NKp46 antibody or incubated with K562 cells in presence of BV421 anti-CD107a antibody and protein transport inhibitor Golgi stop (BD Biosciences) at 37°C with 5% CO2 for 4 hours ...
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bioRxiv - Bioengineering 2023Quote: ... Blood was drawn from healthy volunteers (IRB study #BMB-08-012) into 10 mL CTAD tubes (citrate-based anticoagulant containing the platelet inhibitors theophylline, adenosine, and bipyridamole, BD Vacutainer). Samples were maintained at 4°C and processed within 72 h of collection.
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bioRxiv - Neuroscience 2024Quote: The rate of apoptosis induced by microRNA-29a-5p mimics and inhibitors transfection in astrocytes after OGD/R was determined using Annexin V/PI kit (BD Biosciences). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... we resuspended the cells in 0.6 ml of cold PBS + RNase inhibitor and then filtered the cells with 100-μm cell strainers (Falcon brand, BD Biosciences). Cells were then incubated with fluorophore-conjugated primary antibodies against NeuN and Fos ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... reduced to a single-cell suspension and seeded into a 96-well plate at a density ranging between 1000 – 20 c/w in mTesRTM1 supplemented with 1 nM ROCK inhibitor (BD Biosciences, 562822). Alternatively ...
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bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
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bioRxiv - Cancer Biology 2024Quote: CAR T-cells were incubated with target cells at an effector-to-target (E:T) ratio of 2:1 in complete RPMI 1640 medium containing GolgiStop™ Protein Transport Inhibitor Reagent (BD Bioscience, USA) and CD107a APC antibody (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A, 1 µg/mL and BD Golgi Stop™ containing monensin, 1 µg/mL, BD Biosciences) was added followed by an additional 5-hour incubation at 37°C ...
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bioRxiv - Immunology 2021Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A, 1 µg/mL and BD Golgi Stop™ containing monensin, 1 µg/mL, BD Biosciences) was added for an additional 5 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... The stimulation was performed by incubation for 6 h at 37°C and 5% CO2 in the presence of Protein Transport Inhibitor Cocktail (brefeldin A) (BD Biosciences, San Jose, CA) at a final concentration of 1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by a blood draw for research purposes into a 8.5 ml BD™ P800 blood collection tube coated with potassium EDTA and a proprietary mix of protease and esterase inhibitors (BD Biosciences, cat. no. 366421). The research blood tube was gently mixed and immediately refrigerated until transported to the UWHC Central Lab via cold pack ...