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Citations for Becton, Dickinson and Company :
251 - 300 of 311 citations for Pan Plasmodium Aldolase Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... then cell culture supernatant were harvested and analysed using a BD Cytometric Bead Array (CBA) Human Soluble Protein Master Buffer Kit (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Immunology 2022Quote: ... and 10% heat-inactivated FBS) and incubated at 37°C for 6 hours with protein transport inhibitors GolgiStop and GolgiPlug (BD Biosciences) and S glycoprotein peptide pools 1 μg/mL (JPT product PM WCPV-S-1 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Tissues were incubated at 4°C overnight with the following primary antibodies: monoclonal mouse anti-carboxyl-terminal binding protein 2 (CtBP2) IgG1 at 1:200 (612044; BD Biosciences) counterstained with goat anti-Mouse IgG1 conjugated with Alexa Fluor 568 (#A-21124) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: 344SQ murine lung adenocarcinoma cells expressing firefly luciferase and green fluorescent protein (in 50 μL of 1:1 mix of HBSS and BD Matrigel) were injected into the left lung of 8 weeks old female 129/Sv mice via intrapulmonary injection as described previously (50) ...
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bioRxiv - Immunology 2020Quote: PBMCs or purified T cells were stained for 30 minutes on ice with the antibodies specific for extracellular proteins in Brilliant Stain Buffer (BD, 563794). Following incubation ...
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bioRxiv - Genetics 2020Quote: ... cells were stained with recombinant biotinylated spike protein RBD as described above and analyzed with multiparametric flow cytometry on a FACS Aria III (BD Biosciences) and FlowJo.v10 software (BD Biosciences).
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bioRxiv - Genetics 2020Quote: ... and the amounts of recombinant ACE2-GFP and dACE2-GFP proteins in lysates were quantified by densitometry analysis (Imagelab, BD Biosciences) of Western blots ...
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bioRxiv - Cell Biology 2021Quote: ... and monoclonal cell lines were generated by isolating cells expressing both TauRD fusion proteins in a 96 well-plate by cell sorting with a BD FACS Aria III (BD Biosciences) following amplification ...
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bioRxiv - Cell Biology 2020Quote: ... the cell surface of freshly-prepared epidermal cells was labeled with the following antihuman protein antibodies (clone, manufacturer): FITC–conjugated CD1a (HI149) and PE–conjugated HLA-DR (G46-6, both BD Pharmingen), PE–CF594–conjugated CD3 (UCHT1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Transduced cells were selected with 5μg/ml blasticidin and bulk cells expressing the TetR protein were clonally isolated in 96-well plates using FACS (BD-Aria). BRAFV600E and BRAFWT were expressed in cells using Gateway-compatible lentiviral constructs (pLenti CMV/TO puro DEST ...
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bioRxiv - Microbiology 2021Quote: ... The lung infiltrated cells were cultured with purified RBD protein at 10 μg/ml for 12 h at 37 °C followed by a 6 h treatment with GolgiPlug (BD 555028). After blocking with FcγIII and FcγII receptors antibody (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... CD95, CD278) or intracellular proteins (BCL-2, BCL-6, CD152) and cells were acquired on an LSR II analyser (BD Pharmingen), followed by flow cytometric analysis using the FlowJo Software (FlowJo LLC ...
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bioRxiv - Immunology 2022Quote: ... Cells were then cultured for 2 hours at 37°C in 5% CO2 incubator and followed by the addition of protein secretion inhibitor cocktail containing brefeldin A and monensin (GolgiPlug, BD, 555029) for another 4-hours ...
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bioRxiv - Immunology 2022Quote: ... Spike and RBD tetramers were prepared fresh before use and formed by combining biotinylated proteins with PE-conjugated Streptavidin (BD Biosciences) at a molar ratio of 4:1.
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bioRxiv - Neuroscience 2022Quote: ... that was blocked in 5% non-fat milk for 1 h at room temperature and incubated overnight with primary antibodies against the following proteins: α-synuclein (1:1000, BD), NCK2 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Protein transport inhibitor containing Monensin and protein transport inhibitor containing Brefeldin A were added to cells (both 10 μg/ml, BD Biosciences) and cells cultured for an additional 4 hours ...
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. The surface expression level of S proteins (Fig. 3d) was measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... we first labeled cell surface proteins in FACS buffer for 30 minutes before washing with 1x PBS and fixation with BD Cytofix/Cytoperm (BD Biosciences) for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. The surface expression level of S proteins (Fig. 3d) was measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Phycoerythrin (PE) or peridinin chlorophyll protein -cyanine 5.5 (PerCP-Cy5.5)-conjugated CD3 (BD Biosciences Cat#5 61808, BD Biosciences Cat# 55163); Pacific Blue-conjugated (PB) ...
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bioRxiv - Microbiology 2023Quote: ... was used as a secondary antibody. The surface expression levels of S proteins (Fig. 4A) were measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (FlowJo LLC) ...
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bioRxiv - Neuroscience 2023Quote: Expression of the different fluorescent proteins (ORFs) of the stable GEFRI2 line were measured by High Throughput LSR Fortessa (BD Biosciences) fluorescence-activated cell sorter (FACS) ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with a cocktail of mAbs and four fluorochrome-conjugated spike proteins in staining buffer (2% FBS/PBS) supplemented with Brilliant Stain Buffer Plus (BD Biosciences). The stained cells were fixed (Lysing Solution ...
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bioRxiv - Immunology 2024Quote: ... NK cells were either settled on substrates functionalized with NKp46 antibody or incubated with K562 cells in presence of BV421 anti-CD107a antibody and protein transport inhibitor Golgi stop (BD Biosciences) at 37°C with 5% CO2 for 4 hours ...
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bioRxiv - Immunology 2023Quote: ... Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail, BD Biosciences) and added to the cells ...
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bioRxiv - Evolutionary Biology 2024Quote: ... nuclei were resuspended in 500 μl of wash buffer and labelled with an anti-nuclear pore complex proteins antibody (clone Mab414) and sorted on a FACSAria instrument (BD Biosciences), with a 100 nozzle on a cold block ...
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bioRxiv - Cell Biology 2024Quote: ... and screened for protein levels via Western blots with monoclonal mouse anti-IQGAP1 (1:1000; 610612, BD Biosciences, East Rutherford, NJ), polyclonal rabbit α-tubulin (1:2500 ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfected cells were selected with 5 mg/mL puromycin and sorted based on red fluorescent protein (RFP) by fluorescence-activated cell sorting (FACS) on a FACSAria (BD Biosciences). RFP+ cells were serially diluted in a 96-well plate to generate single cell clones ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in buffer solution (PBS + 2% FBS + 2mM EDTA) and blocked with recombinant Fc protein (1:100 dilution, BD Biosciences, cat#553142). Cells were then incubated with the primary antibody solution (1:100 dilution ...
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bioRxiv - Bioengineering 2020Quote: ... we also preincubated 200 µL of 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Immunology 2020Quote: ... spleen cells were ex vivo stimulated for 20 h with 40 μg/ml PPD (purified protein derivative, Statens Serum Institute, Denmark) in the presence of Golgi Stop™ (BD Biosciences) and Monensin (1000x ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in blocking solution of 1% normal horse serum in PBS with 0.3% Triton X-100 and incubated overnight at 37°C as follows: mouse (IgG1) anti-CtBP2 (C-terminal Binding Protein) 1:200 (#612044, BD Transduction Labs) targeting pre-synaptic ribbons ...
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bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
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bioRxiv - Immunology 2021Quote: ... Intracellular proteins were stained for 60 min at room temperature after permeabilization and fixation with BD Cytofix/Cytoperm Plus (BD Biosciences, 555028) using the following antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Stable cells expressing fluorescent reporter proteins (mCherry or eGFP) were sorted using BD FACSAria Flow Cytometer (BD Bioscience, Mountain View, CA, USA).
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bioRxiv - Cell Biology 2023Quote: ... T cells with residual target protein expression were depleted by antibody staining and bead pull-down or cell sorting (BD Aria III). T cells were re-suspended in MACS buffer (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells expressing the relevant surface receptor or fluorescence protein were sorted using a BD FACSMelody or a BD FACSAria SORP Cell Sorter (BD Biosciences, USA). Luc2 and NLuc expression was confirmed by exposing cells seeded in the wells of a flat-bottom white 96-well plate to 1 volume of 15 mg/mL D-luciferin (#LUCNA-1G ...
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bioRxiv - Cell Biology 2023Quote: ... Protein blots were visualized using Western ECL Substrate Kit (Thermo Pierce) and exposed using a ChemiDoc XRS+ system (BD, Franklin Lakes, NJ). The intensity of bands was quantified by Image J software
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bioRxiv - Cancer Biology 2024Quote: CAR T-cells were incubated with target cells at an effector-to-target (E:T) ratio of 2:1 in complete RPMI 1640 medium containing GolgiStop™ Protein Transport Inhibitor Reagent (BD Bioscience, USA) and CD107a APC antibody (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines created with fluorescently tagged proteins were sorted and normalized for expression using the UC Berkeley Flow Cytometry Facility (BD Bioscience Influx Sorter). Cell lines were confirmed with confocal imaging and immunoblot.
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... Cytokine concentration was quantified in the supernatants by cytometric bead array (CBA) according to the manufacturer’s instructions from the Mouse/Rat Soluble Protein Flex Set System kit (BD Biosciences Pharmingen, Oxford, UK). CBA data was analysed on a MACSQuant analyser with MACSQuantify™ Software and the CBA analysis software package (BD Biosciences).
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bioRxiv - Cancer Biology 2019Quote: ... A polyclonal population of cells expressing all 3 fluorescent proteins (mturquoise2, Venus and mCherry) was subsequently generated by two rounds of FACS (BD FACS Aria II).
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bioRxiv - Immunology 2020Quote: ... Supernatant was collected and secreted TNFα protein levels were measured using a BD OptEIA Mouse TNFα Enzyme-Linked Immunosorbent Assay (ELISA) kit (BD Biosciences, Mississauga, ON). Triplicates wells were used for each stimulation condition ...
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bioRxiv - Immunology 2023Quote: ... Analysis of intracellular antigens was done by using the same fixation and permeabilization as was described under “Metabolic labeling of nascent proteins” or using BD Cytofix/Cytoperm fixation/permeabilization kit (BD Biosciences, cat#554714). Maximum of 3 x 106 cells per reaction ...
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bioRxiv - Immunology 2023Quote: ... Note these experiments were carried out using early access kits from BD Genomics before the implementation of commercially-available single-cell protein/RNA assays (e.g. Feature Barcoding, 10x Genomics; BD Abseq, BD Genomics, Supplemental Table 4), and researchers are recommended to use those newer solutions for any follow-up studies as the techniques and reagents have been refined ...
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bioRxiv - Immunology 2023Quote: ... and the cells were stained with antibodies specific for the following proteins: CD4 (PE Rat Anti-Mouse CD4; Clone RM4-5; BD Biosciences; Cat # 553049), H-2Dd (Anti-mouse H-2Dd antibody ...