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Citations for Becton, Dickinson and Company :
701 - 750 of 1192 citations for PLC gamma 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... for 20LJminutes on ice followed by 2 washes with 1x Perm/ Wash buffer (Cat. No. 554714, BD Biosciences). Primary antibodies were incubated for 1 hour (hr ...
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bioRxiv - Microbiology 2023Quote: ... subtilis strains were grown in 2×SGG [16 g/L nutrient broth (BD Difco, Franklin Lakes, NJ, USA), 1% glycerol ...
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bioRxiv - Bioengineering 2023Quote: ... HUVECs received at P0 were expanded over 2 passages in 100µg/ml collagen 1 (from rat tail, BD) coated flasks in complete EGM-2 medium (Lonza ...
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bioRxiv - Bioengineering 2023Quote: ... 40 µL of 2 days-old main culture were measured using fluorescence-activated cell sorting (BD, FACSAria Fusion) to detect single cells expressing GFP at flow rate of 10.000 evt/s ...
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bioRxiv - Immunology 2024Quote: ... gently fixed at 4°C for 2-4 hours in Cytofix (diluted 1:4 in PBS; BD biosciences), then embedded in O.C.T ...
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bioRxiv - Physiology 2024Quote: ... anti-mouse CD3 primary antibody was used for incubation for 2 hrs at room temperature (BD Biosciences (valid) # 555273 ) ...
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bioRxiv - Neuroscience 2024Quote: ... incubated with 2% normal mouse serum containing an anti-CD16/CD32 antibody (FcγR blocker, BD Pharmingen™ 553142) for 30 min on ice ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained with the following fluorochrome conjugated antibodies: anti-CD3 (SP34–2, Pacific Blue; BD Biosciences), anti-CD8 (SK1 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2 mM EDTA in PBS] or BD Horizon Brilliant Stain Buffer (563794; BD, Franklin Lakes, NJ, USA), and labeled with conjugated fluorescent dye antibodies against CD44 BB700 (745949) ...
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bioRxiv - Immunology 2021Quote: ... 558121), CD11c (clone 3.9, 748288), CD3 (clone SP34-2, 557757) and ITGB7 (clone FIB504, 555945) were purchased from BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: Primary human bronchial epithelial cells (Passage 1 or 2) were expanded on flasks coated with collagen I (BD Biocoat) in bronchial epithelial growth medium (BEGM) ...
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bioRxiv - Bioengineering 2022Quote: Cells were fixed in 2% PFA for 15 minutes and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were incubated with either conformation specific BAK antibody (Clone# G317-2, 1:100) (Cat# 556382, BD Pharmingen) followed by phycoerythrin (PE)-conjugated anti-mouse antibody (1:200 ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed/permeabilized using BD FACS lysing solution and permeabilizing solution 2 according to manufacturer’s instructions (BD Biosciences) followed by intracellular staining with Ki-67-PE and t-bet-BV711 (Biolegend) ...
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bioRxiv - Microbiology 2019Quote: ... and 10 healthy controls (sampled once each) were quantified by two-step sandwich ELISA following the manufacturer’s instructions (Peprotech Human BD-2 and BD-3 ...
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bioRxiv - Genomics 2021Quote: ... stimulated cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Alexa700; BD Biosciences, Custom Bulk 624040, PerCP-Cy5.5; BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... and the following anti-human antibodies were used for surface staining: CD3-BV786 (clone SP34-2, catalog 563800, BD), CD56-BV605 (clone HCD56 ...
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bioRxiv - Cell Biology 2021Quote: ... and the following anti-human antibodies were used for surface staining: CD3-BV786 (clone SP34-2, catalog 563800, BD), CD56-BV605 (clone HCD56 ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular staining was performed using rat anti-mouse IFN-γ-PECy7 (clone XMG1-2, 1:300, BD cat#557649), rat anti-mouse IL-2-PE (clone JES6-5H4 ...
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bioRxiv - Cancer Biology 2020Quote: ... CWR22Rv1 xenografts were established in the flanks of mice by injecting ~2 million cells in 50% matrigel (BD Biosciences). When tumors reached approximately 200mm3 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in blocking buffer (PBS with 2% FBS) containing an anti-BrdU monoclonal antibody (BD Biosciences) (1:100 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were sorted at a concentration of 2 million cells per mL using FACS- Aria-III (BD Biosciences). Forward and side scatter parameters were adjusted so as to eliminate cell clumps and debris ...
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bioRxiv - Immunology 2022Quote: ... mice were injected ip with 200 µL of 10 mg/mL 5-bromo-2’-deoxyuridine (BrdU, BD cat. 559619). Mice were culled by cervical dislocation at the time point indicated in the individual experimental setup ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in 2% fetal-bovine serum in PBS and analyzed using the LSRFortessa flow cytometer (BD Biosciences). A total of 20,000 events were collected for each sample ...
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bioRxiv - Immunology 2022Quote: ... All experimental steps were performed in a 50/50 mixture of PBS + 2% FBS and Brilliant Buffer (BD Bioscience). Antigen probes for Spike ...
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bioRxiv - Developmental Biology 2022Quote: PI-annexin V apoptosis assay was performed at differentiation week 2 and week 4 in normal and HGPS iPSCs-keratinocytes induction according to the manufacturer’s instruction (BD). In brief ...
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bioRxiv - Cancer Biology 2022Quote: ... 2×105 cells were washed with PBS1X and resuspended in 100 µL of Annexin-V binding buffer (BD Biosciences). 1 µL of Annexin-V-APC (Cat# 550474) ...
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bioRxiv - Immunology 2020Quote: ... T cells and DC were stained with anti-syndecan-1 (rat IgG2Aκ monoclonal antibody, clone 281-2, BD Biosciences) or isotype control (rat IgG2Aκ ...
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bioRxiv - Cancer Biology 2021Quote: ... 2% FBS) and analyzed for green and red fluorescence by flow cytometry using a BD FACSymphony instrument (BD-Biosciences).
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bioRxiv - Cancer Biology 2021Quote: ... Spheroids were harvested the next day and transferred in a collagen type I suspension (2 mg/ml) (BD/corning) (354249 ...
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bioRxiv - Immunology 2022Quote: ... SiglecF+) as described in Figure S7: anti-CD45-Brilliant Violet 605 (2 µg/ml, clone 30-F11; BD Bioscience), anti-CD11b APC-Cy7 (1 µg/ml ...
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bioRxiv - Genomics 2020Quote: ... Stained cells were resuspended in FACS buffer (PBSS + 2% FBS) and analyzed by FACS using BD LSRFortessa (BD Bioscience).
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bioRxiv - Immunology 2021Quote: ... Dishes were kept undisturbed for 2 min and the clear supernatant was pipetted out slowly into cell strainer (BD). The filtrate was collected in sterile tubes and the cells were pelleted by centrifugation at 4°C for 10min at 250×g in a centrifuge (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™ ...
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bioRxiv - Developmental Biology 2019Quote: ... were cultured in DMEM/F12 (PAA Laboratories Inc.) supplemented with 2% Ultroser SF (i.e. steroid free; BioSepra) and 1% ITS+ (BD) at 37°C in humidified 5% CO2 and 95% air for 64 hours ...
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bioRxiv - Immunology 2021Quote: T-cell responses were characterized by measurement of the frequency of PBMC expressing IL-2 (PerCP5.5, MQ1-17H12, BD), IL-17a (Alexa700 ...
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bioRxiv - Immunology 2020Quote: PBMCs (1−2×106) were stimulated with epitope-specific peptides (5 μM) and anti-CD28 mAb (0.5 μg/mL, BD) and expanded for 14 days in complete RPMI culture medium containing rIL2 (20 IU/mL ...
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bioRxiv - Immunology 2021Quote: ... All experimental steps were performed in a 50/50 mixture of PBS + 2% FBS and Brilliant Buffer (BD Bioscience). Antigen probes for Spike ...
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bioRxiv - Immunology 2021Quote: ... liver and kidneys were depleted of RBC by 2 minute-incubation in Pharm LyseTM (BD Biosciences, San Jose, CA), washed ...
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bioRxiv - Immunology 2020Quote: ... Cells were seeded (30,000-50,000/wells) into wells pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) along with soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... the yeast were washed twice with FACS buffer (0.5% BSA and 2 mM EDTA in 1x PBS) before analysis via flow cytometry (Accuri C6 flow cytometer, BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... CD3 (SP34-2, Cat# 557757), CD4 (SK3, Cat# 612749), PD1 (EH12.2, Cat# 564104), CTLA4 (BN13, 561717) were obtained from BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... RT) and resuspended in 2 ml of PBS and immediately analyzed by flow cytometry (BD FACS Canto II Instrument). For each sample ...
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bioRxiv - Immunology 2022Quote: ... along with the pre-complexed Beta variant Spike tetramer (2 μg in 100μl) coupled to PE-streptavidin (BD Biosciences). All other aspects with cell sorting ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 2% FBS and filtered through a BD tube with cell strainer (BD Biosciences, Franklin Lakes, NJ, USA) for flow cytometry analysis on LSR II (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD206-PE-Cy5 (clone 19.2; 20 μL; cat. # 551136) and Fixable Viability Stain 700 (2 μL; cat. # 564997) all from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Cells were seeded (30,000-50,000/well) into wells pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) along with soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Biophysics 2022Quote: ... 340495 lot 62981 and 2 μm, custom made, lot MM2327#153.1; #153.2; #153.4; #153.5; #153.6, BD Biosciences, San Jose, CA).
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bioRxiv - Immunology 2022Quote: ... cell suspensions were stimulated with 1μg/ml rIL-2 for 15 min and then fixed and permeabilized with BD Cytofix/cytoperm buffer (BD) and the Phosflow Perm Buffer III (BD ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were stained in 100ul of staining buffer (PBS + 2 % FBS) containing PE Mouse SNAI2/Slug (BD Biosciences 564615), EZH2 eFluor 660 (Thermo Fisher 50-9867-82) ...