Labshake search
Citations for Becton, Dickinson and Company :
201 - 220 of 220 citations for NTR1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... cells were restimulated for 5 hours with the same peptide pool in the presence of brefeldin A (BD Biosciences, San Diego, CA). Cells were then tested for IFN-γ ...
-
bioRxiv - Cell Biology 2021Quote: ... C-peptide and Glucagon labelled cells were detected on an LSRII flow cytometer with FACSDiva™ Software (BD Biosciences, San Jose, CA) using the 405- ...
-
bioRxiv - Immunology 2020Quote: ... up to 5×106 leukocytes were incubated with 5 μg/mL E6 peptide or 1 μg/mL α-CD3e (clone 145-2C11, BD Biosciences) in the presence of 1:1000 Golgi-plug for 5 hours ...
-
bioRxiv - Immunology 2020Quote: ... spleen cells were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug™, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 monoclonal antibody (mAb ...
-
bioRxiv - Immunology 2020Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop™, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
-
bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was done by standard techniques following 5-hour stimulation with Gp61-80 peptide and Brefeldin A (GolgiPlug™, BD Biosciences). No peptide controls were treated under the same conditions supplemented with Brefeldin A but without Gp61-80 peptide ...
-
bioRxiv - Immunology 2020Quote: The following rat anti-mouse antibodies were used for leukocyte analysis within each tissue: purified CD16/CD32 Fc blocking antibody (clone 2.4G2; BD Pharmingen™, San Jose, CA), PerCP-Cy™5.5 Ly6G antibody (clone 1A8 ...
-
bioRxiv - Physiology 2021Quote: ... A 3% BSA-0.2% Triton®X-100 solution was used for permeabilization and blocking followed by a 1 h RT incubation with PBS–1% BSA containing mouse monoclonal DRP1 antibody (611113, BD Transduction Laboratories, 1:50) followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1) after Fc blocking and analyzed using flow cytometry (BD FACS Celesta/Novocyte Advanteon). Cells were fixed and permeabilized using intracellular staining reagents (Intracellular Fixation & Permeabilization Buffer Set ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were fixed in 4% paraformaldehyde and permeabilized using a commercially available saponin-based permeabilization and blocking reagent (1x BD Perm/Wash buffer #554723). Cells were then stained using primary antibodies against Flag (Proteintech 20543-1-AP ...
-
bioRxiv - Immunology 2019Quote: ... Mice were immunized subcutaneously with 50 μg of MOG35-55 peptide emulsified in complete Freund’s adjuvant (CFA, BD Difco, Franklin Lakes, NJ, USA) containing 500 μg of killed Mycobacterium tuberculosis (Strain H37a ...
-
bioRxiv - Immunology 2020Quote: ... Isolated LNMCs were incubated with SIV peptide pools covering gag and env in the presence of co-stimulatory αCD28 and αCD49d (BD, 10 μg/mL) on goat-α-mouse IgG F(ab)2-coated (KPL ...
-
bioRxiv - Immunology 2020Quote: ... and subsequently PBMCs were stimulated in R10 media with specific peptide pools or medium alone (DMSO control) in the presence of 1 μg/ml of α-CD28 (BD bioscience 555725) and α-CD49d (BD bioscience 555501 ...
-
bioRxiv - Immunology 2022Quote: ... PBMCs were stimulated with the RBD peptide pool (5 μg.ml−1 final concentration) and brefeldin A (1 μg.ml−1 ; BD Pharmingen, San Diego, CA, USA) at 37 °C overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated with fluorochrome-labeled monoclonal antibodies after blocking the Fc receptor with anti-CD16/32 monoclonal antibodies (BD Pharmingen, San Diego, CA. USA). Data were analyzed with FlowJo or Kaluza software ...
-
bioRxiv - Neuroscience 2020Quote: EAE was induced in 7-week-old female C57BL/6J mice by subcutaneous flank administration of 200 μg MOG35-55 peptide emulsified with complete Freund’s adjuvant (CFA) (MOG35-55/CFA) (BD Biosciences, San Jose, CA, USA) supplemented with inactive ...
-
bioRxiv - Microbiology 2019Quote: ... ESAT6 peptide (1 μg/ml) or TB10 peptide (1 μg/ml) for 24 hours at 37°C before the addition of GolgiPlug (BD Pharmingen, San Diego, CA). The concentration of protein and peptides used were according to previous reports [19 ...
-
bioRxiv - Immunology 2021Quote: ... Single leucocyte suspensions were stimulated with either phorbol 12-myristate 13-acetate (PMA)-ionomycin or 5 µg/ml of MCMV peptide pool (Table S3) for 6 hours with brefeldin A (BD Biosciences, San Jose, CA). After stimulation ...
-
bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
-
bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...