Labshake search
Citations for Becton, Dickinson and Company :
201 - 250 of 2340 citations for Medium Kit without Serum and without Growth Factors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2019Quote: ... Eggs without egg-shells (to produce ‘naked’ embryos) were obtained from adults by poking with a needle (BD Microlance 3) and raised in Petri dishes coated with 2% agarose (diluted in filtered artificial seawater ...
-
bioRxiv - Molecular Biology 2021Quote: ... cerevisiae strains were grown as indicated in synthetic complete (SC) media (per liter: 1.7 g yeast nitrogen base without ammonium sulfate or amino acids (BD Difco), 5 g ammonium sulfate ...
-
bioRxiv - Microbiology 2021Quote: ... A total of ten thousand events were recorded using a ACEA NovoCyte flow cytometer and analysed without gating using FlowJo software version 10.6.1 (BD Life Sciences).
-
bioRxiv - Genetics 2020Quote: ... strains were grown at 25°C on synthetic complete media (6.7 g/L Yeast Nitrogen Base without Amino Acids (BD #291940)) supplemented with 2% glucose ...
-
bioRxiv - Genomics 2023Quote: ... cerevisiae strains were grown as indicated in synthetic complete (SC) media (per liter: 1.7 g yeast nitrogen base without ammonium sulfate or amino acids (BD Difco), 5 g ammonium sulfate ...
-
bioRxiv - Biochemistry 2023Quote: ... For microscopy experiments SDC-lysine media was used (2% glucose, 6.75 g/l yeast nitrogen base without amino acids (291929, BD Difco), 1.92 g/l yeast synthetic drop-out media supplements without lysine (Y1896 ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were grown in SDC overnight to early log-phase and then washed at least three times in synthetic defined without nitrogen (SD-N) media composed of 0.17% (w/v) yeast nitrogen base without amino acids and ammonium sulfate (BD Difco) and 2% (w/v) ...
-
bioRxiv - Cell Biology 2023Quote: ... Full-length or truncation (ΔTM) of TbPEX15 with and without mutations were cloned in the pPC97 vector containing GAL4- DNA Binding Domain (BD) as described in Table 1 ...
-
bioRxiv - Microbiology 2021Quote: In vitro growth assays were performed in M9 minimal medium (BD) supplemented with 20 mM xylose or glucose (Sigma) ...
-
bioRxiv - Cell Biology 2019Quote: Cells treated with Taxol combine with or without chloroquine were subjected to apoptosis assay according to the manufacturer instructions (BD Biosciences). Briefly ...
-
bioRxiv - Plant Biology 2021Quote: ... The protoplasts were resuspended in the same solution without enzyme and sorted with the cytometry (ARIA III Cell sorter, BD bioscience) with the GFP signal and single-cell pattern to isolate the high purity (~ 100% ...
-
bioRxiv - Plant Biology 2021Quote: ... The AtCGL160 CDS without the signal peptide (see Supplemental Table S2 for primer information) was cloned into the bait vector pGBKT7 (BD-AtCGL160), whereas the coding sequences of CF1-α ...
-
bioRxiv - Immunology 2019Quote: ... Cells were then washed in Phosphate Buffered Saline (PBS) without FBS and stained with Fixable Viability Stain 620 (FVS620; BD Biosciences) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... for 10 minutes at room temperature before stimulation with or without cognate antigen at 10 μg/mL in the presence of 10 μL/mL anti-CD28/49d (BD Biosciences) and 1x protein transport inhibitor (eBioscience ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were submitted to the same procedure as for flow cytometry analysis but without 1% PFA treatment and processed using BD FACSAria (BD Biosciences).
-
bioRxiv - Immunology 2023Quote: ... P33681) without the leader sequence was PCR-amplified and cloned into the pAcGP67 of the BaculoGold Baculovirus Expression System (BD Biosciences). This vector was modified so that all proteins featured a C-terminal H12-tag ...
-
bioRxiv - Immunology 2023Quote: ... prior to stimulation for 5 h with anti-CD3 and anti-CD28 without IL-2 in the presence of brefeldin A and monensin (BD Biosciences).
-
bioRxiv - Microbiology 2023Quote: Giardia lamblia trophozoites (strain WBC6, ATCC 50803) were grown at 37°C without shaking in 16-ml screw-cap plastic tubes (BD Falcon) containing 12 ml modified TYI-S-33 medium supplemented with bovine bile ...
-
bioRxiv - Microbiology 2024Quote: ... overnight cultures of each strain were grown in BHI media and spot plated on agar plates made from tryptic soy broth without added dextrose (TSB-D, BD Difco) supplemented with 3% (wt/vol ...
-
bioRxiv - Cell Biology 2021Quote: ... which were triturated and re-plated on tissue culture dishes coated overnight with growth-factor-reduced Matrigel (BD Biosciences).
-
bioRxiv - Developmental Biology 2022Quote: ... generated EBs were collected and embedded in 50μl (around 40 EBs) of growth-factor-reduced Matrigel® (BD Biosciences), plated in 12-well plates and treated for three days with 1 mg/ml Doxycycline (Sigma) ...
-
bioRxiv - Cancer Biology 2022Quote: ... the transwell inserts from the CIM plates were coated with 20 μl of growth-factor-reduced Matrigel (BD Biosciences), at a concentration of 250 μg/ml ...
-
bioRxiv - Developmental Biology 2021Quote: ... from E16.5 C57BL/6 lungs pooled by litter and cultured on a layer of growth factor reduced Matrigel (BD) in serum-free media (DMEM/F12 supplemented with L-glutamine and penicillin-streptomycin ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tumour xenografts were generated by injection of 1 x 106 cells in 50% growth factor reduced Matrigel (BD Biosciences) into the flanks of CBA.Nude mice ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... rats were sedated under isoflurane anesthesia and tail-vein blood was collected in a volume of approximately 0.5 mL into microtainer tubes without additive (BD, Franklin Lakes, NJ). Tubes were immediately centrifuged at 1000g for 10 min and the serum supernatant was transferred into a labeled storage tube and frozen at −80°C until analyzed for heroin mid-point titer levels as described previously (Bremer et al. ...
-
bioRxiv - Microbiology 2021Quote: ... Synthetic Low Ammonium Dextrose (SLAD) broth was made by combining 0.67 g Yeast Nitrogen Base without amino acids and ammonium sulphate (BD DifcoTM, Herlev, Denmark), 2 g glucose and 50 µl of ammonium sulphate solution (1M ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were processed as for CDC45 but without the methanol fixation step and with a 1:1000 dilution of mouse monoclonal anti-MCM2 (BD Biosciences, 610701) for 14 hours at 4°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 25 μL medium containing 1 × 106 luciferase-expressing MCF10AER/vSrc cells (control or 4-OHT transformed, with or without NLS-catalase expression) were mixed with 75 μL Matrigel matrix (BD Bioscience, #356237) containing 10 μM 4-OHT and injected into the inguinal mammary fat pad of the female mice at 6 to 8 weeks of age ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 2% dextrose) when containing no plasmids and in synthetic defined (SD) media (0.67% yeast nitrogen base without amino acids [BD Difco], 2% dextrose) supplemented with the appropriate yeast synthetic drop-out medium supplement when plasmid maintenance was required (SDΔW-without tryptophan ...
-
bioRxiv - Microbiology 2024Quote: ... Minimal medium was prepared by adding an appropriate carbon source to YNB [0.17% yeast nitrogen base without amino acids and ammonium sulfate (Becton, Dickinson and Company), 0.5% ammonium sulfate] as follows ...
-
bioRxiv - Developmental Biology 2022Quote: ... A density of 100,000 cells per well was used for culture in 8-well #1 coverglass chambers (Labtek) precoated with growth factor reduced Matrigel (80µl, BD Biosciences) for 30 minutes at 37°C ...
-
bioRxiv - Cancer Biology 2021Quote: 1.5×106 pCAF2s expressing shCtl or shPYCR1 and 5×105 MCF10DCIS.com in 200 µl 50% growth factor reduced phenol red free Matrigel (BD Biosciences) in PBS were injected subcutaneously into the flank of 8 week old female BALB/c nude mice (Charles River) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1.5×106 pCAF2 and 5×105 MCF10DCIS.com in 200 µl 50% growth factor reduced phenol red free Matrigel (BD Biosciences) in PBS were injected subcutaneously into the flank of 8 week old female BALB/c nude mice (Charles River) ...
-
bioRxiv - Cancer Biology 2019Quote: The tube formation assay was performed on a 48-well tissue culture dish that was coated with 150 µl growth factor-reduced Matrigel (BD) that was left to solidify at 37°C for 30 min ...
-
bioRxiv - Microbiology 2020Quote: ... Approximately 500 crypts were suspended in 50 μL of growth factor-reduced phenol-free Matrigel (BD Biosciences, San Jose, CA). Next ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1,000 cells were cultured during the length of the experiment in 100 μl basement membrane gels composed of 9.2 μg/μl phenol red-free growth factor reduced Matrigel (BD Bioscience) in 96 well plates ...
-
bioRxiv - Immunology 2022Quote: ... Cells were resuspended in a suitable amount of PBS and mixed at a 1:1 (v:v) ratio with growth factor reduced Matrigel Matrix (BD Biosciences). A final injection volume of 100ul containing 400k cells was injected subcutaneously on the flanks of mice.
-
bioRxiv - Developmental Biology 2021Quote: ... generated by hanging drops culturing of ESCs (1,000 cells per drop) were collected after 4 days and embedded in growth-factor-restricted Matrigel (MTG; BD Biosciences); 50µl MTG drops (containing around 20 EBs ...
-
bioRxiv - Cancer Biology 2020Quote: Sorted tumor cells were seeded at a density of 45 cells per 96/well on Growth factor-reduced Matrigel (BD) and allowed to grow for up to 6 days under standard culture conditions (See cell culture) ...
-
bioRxiv - Cancer Biology 2021Quote: ... HCC1806 cells were seeded at a density of 15,000 cells per well in a 48-well tissue culture plate coated with 125μl of Matrigel Growth Factor Reduced (BD Biosciences). Media was replaced every 72h and growth was monitored for 9 days ...
-
bioRxiv - Cell Biology 2021Quote: ... iPSCs were seeded at 1.25-2.5×104 cells/cm2 onto 12-well plates coated with 80 μg/μl growth factor-reduced Matrigel (BD Biosciences) in mTeSR1 supplemented with 10 μM Y-27632 (Millipore ...
-
bioRxiv - Microbiology 2022Quote: ... 98 mL of fresh MRS medium is then inoculated with 2 mL of the overnight culture and incubated at 37°C without shaking to an OD of 0.5-0.6 (ca. 4-5 hours) in a GasPak anaerobic jar (BD GasPak™ EZ container systems). Cells are harvested a first time by centrifugation at 4,000 g for 5 min ...
-
bioRxiv - Cell Biology 2020Quote: ... or cell carrier 96 wells plates were coated with a thick layer (150-200uL/cm2) of a mix of growth factor-reduced (GFR) Matrigel (BD Biosciences) and neutralized Bovine Collagen I (Purecol ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells (500-700) were seeded into a 500 μL layer of a 1:1 collagen/Growth Factor Reduced MatrigelTM (#354230, Corning/BD Biosciences) mixed in DMEM balance with bicarbonate and were treated twice a week for one month ...
-
bioRxiv - Microbiology 2021Quote: ... 25 μg/ml gentamicin and 100 U/ml penicillin/streptomycin) and were then added to 150 μl of BD Growth Factor Reduced Matrigel Matrix (356230; BD Biosciences). Fifty microliter droplets were added to consecutive wells of a 24-well tissue culture plate (3524 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were plated at a density of 1.0 × 104 cells per well in triplicate in 96-well plates coated with 50 μl of growth factor - reduced Matrigel (BD Biosciences). Plates were incubated overnight at 37° C ...
-
bioRxiv - Bioengineering 2022Quote: ... 2×105 cells were plated in a well of a 12-well plate coated with 80 μg/ml Growth Factor Reduced Matrigel Basement Membrane Matrix (BD Biosciences). One day later ...
-
bioRxiv - Cancer Biology 2021Quote: ... MCF-10A and MCF-10A MYC-ER cells were seeded at a density of 10,000 cells per well in triplicate on a 4-well glass chamber slide coated with Matrigel Growth Factor Reduced (BD Biosciences) as described (Debnath et al. ...
-
bioRxiv - Cancer Biology 2019Quote: ... Collected tissue fragments were counted and plated at a density of 1000 cell fragments per 50 μL of Growth Factor Reduced Matrigel (BD Biosciences) in 24 well plates (Nunc) ...
-
bioRxiv - Neuroscience 2019Quote: ... and a total of 5×105 (50μl) Ret-mCherry sorted (RMS) cells in a 1:1 suspension of PBS with growth factor–reduced Matrigel (356231, BD Biosciences) were injected subdermally to the right dorsal side ...