Labshake search
Citations for Becton, Dickinson and Company :
401 - 444 of 444 citations for IL 4Ra Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... and 5e6 spleen cells were restimulated in vitro with 5e6 irradiated ERAAP KO spleen cells and 20 U/ml of recombinant human IL-2 (BD Biosciences). After 6 days ...
-
bioRxiv - Immunology 2022Quote: ... Supernatants were recovered and the amounts of IL-6 were analyzed by cytokine-specific ELISA kits (BD Biosciences, Mountain View, CA).
-
bioRxiv - Immunology 2023Quote: ... IL-10 and IL-13 secretion using a cytokine bead array (#I558296, #558298, #558302, #558300 and #558349 respectively and all BD Biosciences) on a flow cytometer as recommended by the manufacturer ...
-
bioRxiv - Genetics 2023Quote: ... and CD43-B cells were cultured at a density of 5×105 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). Cultured cells were maintained daily at a density of 0.5-1×106 cells per ml ...
-
bioRxiv - Immunology 2023Quote: ... IL- 10 secretion was determined by ELISA in supernatants harvested after a 24 hour restimulation with anti-CD3 (555336, BD Biosciences) and anti-CD28 (555725 ...
-
bioRxiv - Bioengineering 2023Quote: ... Circulating levels of IL-6 and TNF were determined using a cytokine bead array (BDTM Cytometric Bead Array Mouse IL-6/TNF Flex Set, BD Biosciences). Samples were analyzed in technical triplicates.
-
bioRxiv - Genomics 2023Quote: ... Cell populations expressing the top and bottom 10% IL-1β levels were enriched using a FACSAria Fusion cell sorter (BD Biosciences). At least four million IL-1βlow and IL-1βhigh cells were collected for genomic DNA extraction using Quick-DNA FFPE Kit (Zymo Research) ...
-
bioRxiv - Microbiology 2023Quote: ... and values for IL-2 were determined by using a standard curve generated by using known concentrations of purified recombinant murine IL-2 (BD Biosciences).
-
bioRxiv - Immunology 2023Quote: ... prior to stimulation for 5 h with anti-CD3 and anti-CD28 without IL-2 in the presence of brefeldin A and monensin (BD Biosciences).
-
bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL, BD Biosciences, Franklin Lakes, NJ, USA). A 1:1,000 dilution of anti-mouse IgG Alexa-647 (stock 1 mg/mL ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were then stained for intracellular IL-6 and events were collected using the BD LSR Fortessa cell analyzer (BD Biosciences). Data was analyzed using the FlowJo Software (BD Biosciences).
-
bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells incubated with IL-10 at 37 °C were fixed and permeabilized for 20 min on ice with Cytofix/Cytoperm (BD Biosciences). Cells were washed twice in permeabilization buffer and further stained with anti-mouse IL-10 in permeabilization buffer for 30 min at on ice ...
-
bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences ...
-
bioRxiv - Cell Biology 2021Quote: Serum cytokine levels (Ifn-γ, Il-1β and Tnf) were determined with a CBA Flex Set Multiplex Cytokine Bead Array (BD Biosciences).
-
bioRxiv - Immunology 2022Quote: ... and the supernatants were harvested for cytokine detection (IL-10 and TGF-β) by specific ELISAs using a standard protocol (BD OptEIA). The detection limits for IL-10 is 31.3–2000 pg/ml e TGF- β is 62.5–4,000 pg/mL) ...
-
bioRxiv - Immunology 2020Quote: ... IL-2 and IFN-γ concentration secreted in cell supernatant were measured using sandwich ELISA according to manufacturer’s protocol (BD Biosciences, USA). Plates were read on a Varioskan Lux multimode microplate reader (ThermoFisher ...
-
bioRxiv - Immunology 2022Quote: Pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured in tissue culture supernatants using a commercially available sandwich ELISA kit (BD Biosciences). As recommended by the manufacturer ...
-
bioRxiv - Microbiology 2019Quote: ... The cell-free supernatants were assayed for IL-1β and TNFα using a commercial Enzyme-Linked Immunosorbent Assay (ELISA) Kit (BD Bioscience-Pharmingen) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Secreted concentrations of IL-13 and IFN- γ were measured from cell supernatant using a cytometric bead array (CBA, BD Biosciences, UK) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: The concentration of cytokine IL-12 present in serum samples was evaluated using the “Cytometric Bead Array” kit (CBA, BD Bioscience, USA). The analyses of these cytokines were performed using flow cytometry and the data analyzed using FCAP Array software.
-
bioRxiv - Immunology 2023Quote: ... PE-cy7-conjugated anti-TNF-α (Clone MP6-XT22, 1:800), and APC-conjugated anti-IL-4 (Clone 11B11, 1:100) (BD Biosciences). All stained samples were acquired using an LSR-Fortessa flow cytometer or Symphony A3 (Becton Dickinson ...
-
bioRxiv - Immunology 2023Quote: ... Cytometric Bead Array using enhanced sensitivity flex sets of human IL-2/IFN- γ/TNF- α was done following manufacturer’s instruction (BD Biosciences). Alternatively ...
-
bioRxiv - Cancer Biology 2023Quote: ... IFN-γ and TGF-β in mouse serum was performed with the BD OptEIATM Mouse IL-10 ELISA Set (BD Biosciences, 555252), BD OptEIATM Mouse TNF (Mono/Mono ...
-
bioRxiv - Bioengineering 2023Quote: ... for 20min at 4°C and stained with intracellular antibodies including anti-mouse IFN-γ-Pe-Cy7 and IL-2-BV605 (BD Bioscience) for 30min at 4°C ...
-
bioRxiv - Physiology 2019Quote: Liver tissues were weighed and homogenized in assay diluent provided by the TNF-α or IL-6 ELISA kit (BD, Franklin Lakes, NJ). Plasma samples were collected with heparin as anticoagulant ...
-
bioRxiv - Immunology 2022Quote: ... were added to the ELISpot plate containing an immobilized primary antibody to either IFN-g or IL-4 (BD Cat# 551881 and BD Cat# 551878, respectively), and were exposed to various stimuli (e.g ...
-
bioRxiv - Cell Biology 2019Quote: ... the plasma levels of pro-inflammatory cytokines IL-6 and TNFα were quantified by bead-based flow cytometry assay (CBA Kit; BD Biosciences, Heidelberg, Germany) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2021Quote: ... Purified rS proteins were evaluated by 4-12% gradient SDS-PAGE stained with Gel-Code Blue reagent (Pierce, Rockford, IL) and purity was determined by scanning densitometry using the OneDscan system (BD Biosciences, Rockville, MD).
-
bioRxiv - Microbiology 2020Quote: ... Purified S-proteins were evaluated by 4-12% gradient SDS-PAGE stained with Gel-Code Blue reagent (Pierce, Rockford, IL) and purity was determined by scanning densitometry using the OneDscan system (BD Biosciences, Rockville, MD).
-
bioRxiv - Cell Biology 2022Quote: ... 10 min at room temperature) and the supernatants were collected to measure the levels of IL-2 by ELISA using the OptEIA™ kit (BD Biosciences, USA), according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2023Quote: ... and interleukin-2 cytokine production was measured using BD optiEA Mouse IL-2 ELISA set following the manufacturer’s instructions (BD Biosciences, Cat no. 555148).
-
bioRxiv - Immunology 2023Quote: ... α-CD44:BV570 (IM7), α-FoxP3:AlexaFluor647 (150D), α-IFN-γ:BV605 (XMG1.2), α-IL-17:FITC (eBio17B7, eBioscience) or α-TCRβ:FITC (H57597, BD Pharmigen). For intracellular staining ...
-
bioRxiv - Microbiology 2023Quote: ... and IL-18 were measured in mouse colonic tissue extracts by using the BD OptEIA ELISA System (BD Biosciences, San Jose, CA, USA). All assays were performed as per the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2021Quote: ... Stimulated cells were stained with fluorochrome-conjugated anti-MCP-1 and anti-IL-6 Abs using the Cytofix/Cytoperm kit (BD Biosciences, San Jose, CA) to determine MCP-1 and IL-6 production ...
-
bioRxiv - Immunology 2019Quote: ... IL-10 and IL-17α were determined in plasma samples through the Mouse Th1/Th2/Th17 Cytometric Bead Array kit (BD Biosciences, San Jose, CA). Bead fluorescence was measured using the BD Biosciences LSRII flow cytometer and analyzed with the accompanying software ...
-
bioRxiv - Immunology 2022Quote: The levels of cytokines in cell culture medium were determined with BD OptEIA™ Mouse IL-6 ELISA set (BD Biosciences, Franklin Lakes, NJ), ELISA MAX™ Standard Set Mouse IL-10 (BioLegend) ...
-
bioRxiv - Immunology 2023Quote: ... Cells were then stained with fluoresceinated antibodies against (TCRý, CD4, CD8, CD3, CD3χ, CXCR5, PD1, and intracellularly for ZAP70 and IL-10) (BD Biosciences, San Jose, CA) and data acquired on a FORTESSA flow cytometer and analyzed using FlowJo Software (Ashland ...
-
bioRxiv - Immunology 2019Quote: ... Serum was collected by tail-tipping before LPS injection and 2 and 4 hours following injection for IL6 determination by ELISA (BD; OptEIATM Set Mouse IL-6), as per manufactures instructions.
-
bioRxiv - Immunology 2021Quote: ... and intracellular cytokines (PE anti-mouse IFN-γ, PE-Cy®7 anti-mouse TNF-α, APC anti-mouse IL-2, all from BD Biosciences, USA). The labeled cells were acquired with the BD FACSCanto™ II flow cytometer and analyzed with the program FlowJo v.10.6.2 (BD Biosciences).
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... we allowed organotypic cultures to differentiate at an ALI in a defined differentiation media supplemented with IL-13 [base media with 2% v/v NuSerum (BD BioSciences, San Jose, CA, USA), 50 nM retinoic acid (Sigma-Aldrich ...
-
bioRxiv - Immunology 2023Quote: ... 5.0 × 104 CD4+ T cells were serially diluted onto either α-IL-2 or α-IFNɣ sterile coated PVDF 96 well plates (BD Biosciences, NJ, USA). The CD4+ T cells were then activated for 24 or 72 h in complete DMEM ...
-
bioRxiv - Immunology 2023Quote: ... and tumour necrosis factor α concentrations were quantified in lung homogenates using an ELISA kit (BD Opt EIA Mouse IL-6 ELISA Sets, #555240; BD Biosciences Franklin Lakes, NJ, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... and anti-inflammatory cytokines (IL-10, TGF-β) were determined in plasma by ELISA at the indicated time points (OptEIA set; BD Biosciences, San Diego, CA, USA) according to the manufacturer’s instructions ...