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151 - 200 of 1657 citations for IL 22 Human HEK293 since 2019

• Antigen-driven PD-1⁺TOX⁺EOMES⁺ and PD-1⁺TOX⁺BHLHE40⁺ synovial T lymphocytes regulate chronic inflammation in situ

  Patrick Maschmeyer, et al., bioRxiv - Immunology 2019

  Quote: ... IL-21 PE (clone 3A3-N2.1, BD), IL-10 APC (clone JES3-19F1 ...
• The neonatal microenvironment programs conventional and intestinal Tbet⁺ γδT17 cells through the transcription factor STAT5

  Darshana Kadekar, et al., bioRxiv - Immunology 2019

  Quote: ... IL-17-BV786 (TC11-18H10, BD biosciences), IL-22-PE (1H8PWSR ...
• Dynamic regulation of CD45 by tetraspanin CD53

  V. E. Dunlock, et al., bioRxiv - Immunology 2019

  Quote: ... anti-mouse IL-2 (JES6-1A12, BD), anti-mouse IL-2 biotin (JES6-5H4 ...
• Th22 cells are a major contributor to the mycobacterial CD4+ T cell response and are depleted during HIV infection

  Rubina Bunjun, et al., bioRxiv - Immunology 2019

  Quote: ... IL-17 Alexa488 (N49-653; BD Biosciences), IL-17 FITC (BL-168 ...
• Tumor-derived extracellular vesicles regulate tumor-infiltrating regulatory T cells via the inhibitory immunoreceptor CD300a

  Yuta Nakazawa, et al., bioRxiv - Cancer Biology 2020

  Quote: ... 20 ng/ml IL-2 (BD Pharmingen), and 2.5 ng/ml TGF-β (R&D system ...
• Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Dextran Sodium Sulfate-Induced Ulcerative Colitis

  Mingyue Li, et al., bioRxiv - Microbiology 2021

  Quote: ... IL-17A-PE (BD Biosciences, Cat# 561020), IFN-γ-APC (BD Biosciences ...
• Immunogenicity and In vivo protection of a variant nanoparticle vaccine that confers broad protection against emerging SARS-CoV-2 variants

  James Logue, et al., bioRxiv - Immunology 2021

  Quote: ... BV711-conjugated anti-IL-13 (BD Biosciences), and the yellow LIVE/DEAD® dye.
• TCR affinity controls the dynamics but not the functional specification of the Th1 response to mycobacteria

  Nayan D Bhattacharyya, et al., bioRxiv - Immunology 2020

  Quote: ... IL-4 (11B11) (both from BD Biosciences); T-bet (4B10 ...
• A self-amplifying RNA vaccine against COVID-19 with long-term room-temperature stability

  Emily A. Voigt, et al., bioRxiv - Immunology 2022

  Quote: ... or IL-5 (BD Biosciences, #51-1805KZ) capture antibodies at a 1:200 dilution in ELISpot coating buffer (eBioscience ...
• Attenuated Mycobacterium tuberculosis Vaccine Protection in a Low Dose Murine Challenge Model

  Samuel J. Vidal, et al., bioRxiv - Microbiology 2023

  Quote: ... and IL-4 (clone 11B11, BD Biosciences).
• Defining antigen targets to dissect vaccinia virus (VACV) and Monkeypox virus (MPXV)-specific T cell responses in humans

  Alba Grifoni, et al., bioRxiv - Immunology 2022

  Quote: ... IL-2 BB700 (1:25, BD, 566405), IFNγ (1:100 ...
• Inulin prebiotic reinforces host cancer immunosurveillance via γδ T cell activation

  Emilie Boucher, et al., bioRxiv - Immunology 2022

  Quote: ... and IL-17A (TC11-18H10) (BD Biosciences) (Table S2) ...
• Intranasal self-amplifying RNA SARS-CoV-2 vaccine produces protective respiratory and systemic immunity and prevents viral transmission

  Madeleine F. Jennewein, et al., bioRxiv - Immunology 2023

  Quote: ... or IL-5 (BD Biosciences #51-1805KZ) at a 1:200 dilution in Dulbecco’s PBS (DPBS ...
• E-cigarette vapour induces cellular senescence in primary lung fibroblasts and may contribute to lung pathology

  Jack Bozier, et al., bioRxiv - Cell Biology 2023

  Quote: ... Supernatants (for IL-8 ELISA, BD Biosciences) and RNA extracts were collected after 24 hours of stimulation ...
• Reciprocal regulation between cell mechanics and ZO-1 guides tight junction assembly and epithelial morphogenesis

  Alexis J. Haas, et al., bioRxiv - Cell Biology 2022

  Quote: ... 22 and 10mm coverslips were then incubated with a solution of 65μg/ml Matrigel (BD Biosciences) for 2.5 hours at 37 °C and 13mm coverslips were silanized for 3 minutes using ethanol containing 0.37% Bind-Silane solution (GE Healthcare Life Science ...
• Patient-derived xenografts and single-cell sequencing identifies three subtypes of tumor-reactive lymphocytes in uveal melanoma metastases

  Joakim Karlsson, et al., bioRxiv - Cancer Biology 2023

  Quote: ... MART1 specific yTILs were identified as previously described 22 and sorted using FACSAria III (BD Biosciences). yTILs were expanded using a standard REP (Rapid Expansion Protocol) ...
• A secreted Echinococcus multilocularis activin A homologue promotes regulatory T cell expansion

  Justin Komguep Nono, Manfred B. Lutz, Klaus Brehm, bioRxiv - Immunology 2019

  Quote: ... T-cells supernatants were probed for IL-10 release by ELISA as per the manufactureŕs instructions (BD OptEIA - Mouse IL-10 ELISA Set - BD Biosciences with a detection limit of 19pg/ml).
• Preserving Vascular Integrity Protects Mice Against Multidrug-Resistant Gram-Negative Bacterial Infection

  Teclegiorgis Gebremariam, et al., bioRxiv - Microbiology 2020

  Quote: The inflammatory cytokine response (IL-1β, IL-6, and TNF-α) was determined using Cytometric Bead Array (BD Biosciences) per the manufacturer’s instructions.
• Dendritic cells require TMEM176A/B ion channels for optimal MHC II antigen presentation to naive CD4⁺ T cells

  Melanie Lancien, et al., bioRxiv - Immunology 2020

  Quote: ... for 16 h before the quantification of IL-12p40 and IL-6 in the supernatant by ELISA (BD Biosciences). For phenotypic analysis (MHC II ...
• Meningeal IL-17 producing T cells mediate cognitive impairment in salt-sensitive hypertension

  Monica M. Santisteban, et al., bioRxiv - Neuroscience 2023

  Quote: IL-17 concentration in serum was measured by cytometric bead array mouse IL-17A Enhanced Sensitivity Flex Set (BDBiosciences)24 or by electrochemiluminescence-based multi-array MSD V-Plex Mouse IL-17A Kit (MesoScale)88 ...
• Structure-based inhibitors reveal roles for the clathrin terminal domain and its W-box binding site in CME

  Zhiming Chen, et al., bioRxiv - Cell Biology 2020

  Quote: ... anti-human CD44 or anti-human CD59 (BD Pharmingen) or 5μg/ml Biotin-Tfn (Sigma) ...
• Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet

  KaLynn Harlow, et al., bioRxiv - Developmental Biology 2019

  Quote: ... A 22 g x 1.5-inch needle and a 3 ml serum Vacutainer® (BD Life Sciences) tube was used to collect blood by jugular venipuncture ...
• Phospho-tuning immunity through Denisovan, modern human and mouse TNFAIP3 gene variants

  Nathan W. Zammit, et al., bioRxiv - Immunology 2019

  Quote: ... Serum was also collected by cardiac puncture for measurement of IL-6 by ELISA (BD; OptEIATM Set Mouse IL-6), as per manufacturer’s instructions ...
• Development of a Synthetic Poxvirus-Based SARS-CoV-2 Vaccine

  Flavia Chiuppesi, et al., bioRxiv - Immunology 2020

  Quote: ... In experiments testing double recombinants SARS-CoV2 vectors IL-2 antibody was not included and PE-CF594 anti-mouse IL-4 (clone 11B11, 562450, BD) and BV421 rat anti mouse IL-10 (clone JES5-16E3 ...
• MeCP2 deficiency exacerbates the neuroinflammatory setting and autoreactive response during an autoimmune challenge: implications for Rett Syndrome

  MI Zalosnik, et al., bioRxiv - Neuroscience 2020

  Quote: ... IL-10 and IL-17 were analyzed using the Cytometric Bead Array (CBA) Mouse Th1/Th2 Cytokine Kit (BD Biosciences) according to manufacturer’s instructions.
• Proresolving mediators LXB₄ and RvE1 regulate inflammation in stromal cells from patients with shoulder tendon tears

  Stephanie G Dakin, et al., bioRxiv - Cell Biology 2019

  Quote: IL-6 is an important cytokine implicated in inflammation and is abundantly released by tendon stromal cells isolated from patients with shoulder tendon tears after stimulation with IL-1β15 IL-6 in tissue culture supernatants was measured using enzyme-linked immunosorbent assay (ELISA) reagents (BD Biosciences) using incubations isolated from 5 donors ...
• Transient protein accumulation at the center of the T cell antigen presenting cell interface drives efficient IL-2 secretion

  Danielle J. Clark, et al., bioRxiv - Immunology 2019

  Quote: ... The cells were then incubated for 18 hours and IL-2 amounts in the supernatant were determined using a mouse IL-2 OptEIA ELISA kit from BD Biosciences as per manufacturer’s instructions.
• Identification and characterization of an HtrA sheddase produced by Coxiella burnetii

  Ikram Omar Osman, et al., bioRxiv - Microbiology 2023

  Quote: The release of IL-10 and IL-6 were quantified in cell supernatants using specific immunoassay kits purchased from BD Biosciences ...
• Intranasal self-amplifying RNA SARS-CoV-2 vaccine produces protective respiratory and systemic immunity and prevents viral transmission

  Madeleine F. Jennewein, et al., bioRxiv - Immunology 2023

  Quote: ... detection antibody (IFNγ, BD Biosciences #51-1818KA; IL-17A, Invitrogen #88-7371-88; and IL-5, BD Biosciences #51-1806KZ) was diluted in ELISpot diluent (eBiosciences ...
• Nanoengineered DNA origami with repurposed TOP1 inhibitors targeting myeloid cells for the mitigation of neuroinflammation

  Keying Zhu, et al., bioRxiv - Immunology 2021

  Quote: ... and Mouse IL-10 Flex Set (BD, 558300). The cytokine level in the supernatant was reflected by the median fluorescence intensity (MFI ...
• IL-21 signaling promotes the establishment of KSHV infection in human tonsil lymphocytes by increasing early targeting of plasma cells

  Nedaa Alomari, et al., bioRxiv - Microbiology 2022

  Quote: ... and IL-21 receptor (2μl/test BD 330114). For baseline T cell frequencies ...
• CD8+ lymphocytes modulate Zika virus dynamics and tissue dissemination and orchestrate antiviral immunity

  Blake Schouest, et al., bioRxiv - Immunology 2019

  Quote: ... IL-2 (PCP-Cy5.5; MQ1-17H12; BD Biosciences), and IFNg (AL647 ...
• Development of a Synthetic Poxvirus-Based SARS-CoV-2 Vaccine

  Flavia Chiuppesi, et al., bioRxiv - Immunology 2020

  Quote: ... and PE-CF594 anti-mouse IL-2 (BD Biosciences (Clone JES6-5H4 ...
• SARS-CoV-2 spike glycoprotein vaccine candidate NVX-CoV2373 elicits immunogenicity in baboons and protection in mice

  Jing-Hui Tian, et al., bioRxiv - Microbiology 2020

  Quote: ... and APC-conjugated anti-IL-4 (BD Biosciences). All stained samples were acquired using a LSR-Fortessa flow cytometer (Becton Dickinson ...
• mRNA-1273 or mRNA-Omicron boost in vaccinated macaques elicits comparable B cell expansion, neutralizing antibodies and protection against Omicron

  Matthew Gagne, et al., bioRxiv - Immunology 2022

  Quote: ... IL-13 BV421 (clone JES10-5A2, BD Biosciences), IL-17 BV605 (clone BL168 ...
• mRNA-1273 or mRNA-Omicron boost in vaccinated macaques elicits comparable B cell expansion, neutralizing antibodies and protection against Omicron

  Matthew Gagne, et al., bioRxiv - Immunology 2022

  Quote: ... IL-2 BV750 (clone MQ1-17H12, BD Biosciences), IL-4 BB700 (clone MP4-25D2 ...
• mRNA-1273 or mRNA-Omicron boost in vaccinated macaques elicits comparable B cell expansion, neutralizing antibodies and protection against Omicron

  Matthew Gagne, et al., bioRxiv - Immunology 2022

  Quote: ... IL-21 Ax647 (clone 3A3-N2.1, BD Biosciences) and CD154 BV785 (clone 24-31 ...
• mRNA-1273 or mRNA-Omicron boost in vaccinated macaques elicits comparable B cell expansion, neutralizing antibodies and protection against Omicron

  Matthew Gagne, et al., bioRxiv - Immunology 2022

  Quote: ... IL-4 BB700 (clone MP4-25D2, BD Biosciences), TNF-FITC (clone Mab11 ...
• Role of Rv3351c in trafficking Mycobacterium tuberculosis bacilli in alveolar epithelial cells and its contribution to disease

  Megan Prescott, et al., bioRxiv - Microbiology 2020

  Quote: ... or IL-8 (BD Pharmingen, Franklin Lakes, NJ) according to manufacturers’ protocols.
• Protection against SARS-CoV-2 Beta Variant in mRNA-1273 Boosted Nonhuman Primates

  Kizzmekia S. Corbett, et al., bioRxiv - Immunology 2021

  Quote: ... IL-2 BV750 (clone MQ1-17H12, BD Biosciences), IL-4 BB700 (clone MP4-25D2 ...
• Protection against SARS-CoV-2 Beta Variant in mRNA-1273 Boosted Nonhuman Primates

  Kizzmekia S. Corbett, et al., bioRxiv - Immunology 2021

  Quote: ... IL-4 BB700 (clone MP4-25D2, BD Biosciences), TNF-FITC (clone Mab11 ...
• A glimpse into the diverse cellular immunity against SARS-CoV-2

  Cheng-Wei Chang, et al., bioRxiv - Microbiology 2021

  Quote: ... TNFα and IL-2 (all from BD Pharmingen) at 4°C for 20 min ...
• Unaltered T cell responses to common antigens in individuals with Parkinson’s disease

  Gregory P. Williams, et al., bioRxiv - Immunology 2022

  Quote: ... IL-2-BB700 (BD, clone MQ1-17H12, RRID:AB_2744488), IL-10-PE-Dazzle594 (Biolegend ...
• Prime-pull immunization of mice with a BcfA-adjuvanted vaccine elicits mucosal immunity and prevents SARS CoV-2 infection and pathology

  Mohamed M. Shamseldin, et al., bioRxiv - Immunology 2022

  Quote: ... IL-5 APC (clone TRFK5) (BD Cat. 554396). For examination of CD8+ population ...
• Dynamic regulation of CD45 by tetraspanin CD53

  V. E. Dunlock, et al., bioRxiv - Immunology 2019

  Quote: ... anti-mouse IL-2 biotin (JES6-5H4, BD),CellTrace Violet (Thermo Fisher Scientific).
• Immunogenicity and In vivo protection of a variant nanoparticle vaccine that confers broad protection against emerging SARS-CoV-2 variants

  James Logue, et al., bioRxiv - Immunology 2021

  Quote: ... and APC-conjugated anti-IL-4 (BD Biosciences). All stained samples were acquired using a LSR-Fortessa or a FACSymphony flow cytometer (Becton Dickinson ...
• Genetic editing of CISH enhances T cell effector programs independently of immune checkpoint cell surface ligand expression

  Elisa Arthofer, et al., bioRxiv - Cancer Biology 2021

  Quote: ... IL-2 (MQ1-17H12, 1:40) (BD Bioscience), or TNF-α (MAb11 ...
• Protection from SARS-CoV-2 Delta one year after mRNA-1273 vaccination in nonhuman primates is coincident with an anamnestic antibody response in the lower airway

  Matthew Gagne, et al., bioRxiv - Immunology 2021

  Quote: ... IL-21 Ax647 (clone 3A3-N2.1, BD Biosciences), and CD154 BV785 (clone 24-31 ...
• Protection from SARS-CoV-2 Delta one year after mRNA-1273 vaccination in nonhuman primates is coincident with an anamnestic antibody response in the lower airway

  Matthew Gagne, et al., bioRxiv - Immunology 2021

  Quote: ... IL-13 BV421 (clone JES10-5A2, BD Biosciences), IL-17 BV605 (clone BL168 ...
• Protection from SARS-CoV-2 Delta one year after mRNA-1273 vaccination in nonhuman primates is coincident with an anamnestic antibody response in the lower airway

  Matthew Gagne, et al., bioRxiv - Immunology 2021

  Quote: ... IL-4 BB700 (clone MP4-25D2, BD Biosciences), TNF-FITC (clone Mab11 ...