Labshake search
Citations for Becton, Dickinson and Company :
601 - 650 of 718 citations for Hrms Rapid Pcb Screening Calibration Solution Cs0.05 Unlabeled 13C12 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... Cells were washed twice and incubated overnight at 4°C with the fluorochrome-conjugated antibodies and unconjugated rat and mouse IgG in Perm/Wash solution (BD Biosciences), which was followed by additional 5 min incubation in Perm/Wash solution without antibodies ...
-
bioRxiv - Cell Biology 2022Quote: ... 10 μl of platelet suspension was mixed with 40 μl of staining solution (HEPES-Tyrode buffer supplemented with 2.5 mM CaCl2, 1:50 PE rat anti-mouse CD41 antibody (BD Biosciences 561850), and 3:50 FITC Annexin V (BD Biosciences 556419)) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton-X (proliferating abl pre-B cells) or 0.5% Triton-X (MCF10A cells) in PBS and fixed with BD Cytofix/Cytoperm solution (BD Biosciences, #554722) containing 4.2% formaldehyde ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were stained using 8 volumes of stain solution per volume of culture (2% HCT) and analysed on a LSR-II Flow Cytometer (BD Biosciences). Statistical significance was determined by the two-sample Wilcoxon rank-sum (Mann-Whitney ...
-
bioRxiv - Immunology 2019Quote: ... Samples for flow cytometry were fixed in 2% paraformaldehyde solution in PBS and analyzed using a LSRII flow cytometer (BD Biosciences) and FlowJo software (Tree Star ...
-
bioRxiv - Immunology 2019Quote: ... The surface staining was performed in PBS for 15 minutes at room temperature in the dark and subsequently fixed using Permeabilizing Solution 2 (BD Biosciences) for 10 minutes at room temperature.
-
bioRxiv - Bioengineering 2021Quote: ... whole blood was drawn from healthy adult volunteers into acid citrate dextrose anticoagulant tubes (ACD, Solution A; BD Franklin Lakes, NJ). Consent from the volunteers was obtained prior to the blood collection ...
-
bioRxiv - Immunology 2020Quote: ... and cells were fixed and permeabilized at room temperature for 15 min with Fixation/Permeabilization Solution Kit (BD Biosciences; cat. #554714). For each sample a minimum of 1.2x105 stopping gate events (live CD3+ T-cells ...
-
bioRxiv - Cancer Biology 2019Quote: ... cells were trypsinized, filtered through a 70 μm strainer (Laboteca; McAllen, TX) and fixed/permeabilized using the Cytofix/Cytoperm Solution Kit (BD Biosciences), according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2021Quote: ... The protoplasts were resuspended in the same solution without enzyme and sorted with the cytometry (ARIA III Cell sorter, BD bioscience) with the GFP signal and single-cell pattern to isolate the high purity (~ 100% ...
-
bioRxiv - Cancer Biology 2021Quote: ... A primary antibody dilution was prepared in the blocking solution with the antibodies diluted 1:200 for HIF1α (BD Bioscience, 610958), 1:50 for phosphor-S6 (CST ...
-
bioRxiv - Bioengineering 2022Quote: The samples were diluted in proportion of 0.5% with PBS and 10 μL of the diluted solution were sampled in every measurement using a flow cytometer (LSRFortessa4, BD, USA). GFP were excited at 488 nm ...
-
bioRxiv - Microbiology 2020Quote: ... LB-Miller agar solutions (molten) were prepared by combining LB-Miller medium with agar (final concentration of 1.5% w/v) (BD Difco™) and autoclaving ...
-
bioRxiv - Microbiology 2020Quote: ... these cells were stimulated with a 10 μg/ml solution of CD28 and CD49d co-stimulatory antibodies (both from BD Biosciences) and 1ug/ml of 15-mer overlapping peptide pools spanning either the SARS-CoV-2 spike (S) ...
-
bioRxiv - Cancer Biology 2021Quote: 3D-grown MCF-10A and MCF-10A MYC-ER cells were washed with PBS (1X) and the Matrigel was dissolved by incubating slides at 4°C in Cell Recovery Solution (BD Biosciences). Cells were lysed in Laemmli buffer (50 mM Tris-HCl pH 6.2 ...
-
bioRxiv - Cancer Biology 2021Quote: 3D-grown MCF-10A cells were washed with PBS (1X) and the Matrigel was dissolved by incubating slides at 4°C in Cell Recovery Solution (BD Biosciences). 2D-grown HEK293T ...
-
bioRxiv - Immunology 2022Quote: ... Intracellular staining was performed after treatment with Fixation/Permeabilization solution and in the presence of Perm/Wash buffer (Cytofix/ Cytoperm kit, BD Biosciences) using anti-mouse IFN-γ FITC ...
-
bioRxiv - Microbiology 2019Quote: ... 15% hydrogen peroxide in 1X PBS) was added to the test tubes and the solution was mixed using disposable 10 μL loops (BD Difco). Tubes were incubated at room temperature until gas production subsided (approximately 5-10 minutes) ...
-
bioRxiv - Immunology 2019Quote: ... Intracellular cytokine staining was performed to examine the production of cytokines in these CAR-T cells using Fixation/Permeabilization Solution Kit (BD Biosciences). CD107 expression was determined as described (Liu et al. ...
-
bioRxiv - Immunology 2019Quote: ... centrifuged at 450 x g for 10 min and washed twice in saline solution and stained with fixable viability stain FVS V450 (BD Biosciences) at the concentration 1:1000 for 15 min at room temperature ...
-
bioRxiv - Cell Biology 2019Quote: Cell were incubated 20 min in culture media completed with a staining solution composed of MitoTracker Green FM (100 nM) (BD Horizon), TMRE (ultra pure ...
-
bioRxiv - Cell Biology 2019Quote: ... and then probed overnight at 4°C with the respective primary antibodies diluted in powdered milk block solution at 2.5g/50mL of TBST: mouse anti-CD9 (BD Pharmigen #555370) 1:1000 ...
-
bioRxiv - Cancer Biology 2019Quote: ... cells were stained by replacing the solution containing ethanol with another made up of PBS/FBS and implemented with PI (1:100, BD Bioscience) and RNaseA (1:400 ...
-
bioRxiv - Microbiology 2021Quote: Mouse spleens were collected in ice-cold storage solution (PBS 2% FBS) and single-cell suspensions prepared using cell strainers (BD-Falcon). Cells were washed upon red blood cells lysis (150 mM NH4Cl ...
-
bioRxiv - Bioengineering 2021Quote: ... We incubated the ECs in a 1:500 primary antibody solution containing Mouse anti-CD144 (VE-Cad, BD Pharmingen Cat. #55561) in ADB overnight at 4 oC ...
-
bioRxiv - Immunology 2020Quote: ... mouse anti human ICAM-1 (CD54) and mouse anti human VCAM-1 (CD106) antibodies and fixed with FACS lysing solution (BD Bioscience). Surface levels were determined by flow cytometry (Gallios ...
-
bioRxiv - Genetics 2021Quote: ... at least 2x106 iPSC-PPC cells were fixed and permeabilized using the Fixation/Permeabilization Solution Kit with BD GolgiStopTM (BD Biosciences) following manufacturer recommendations ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... activation of T-cells was measured by flow cytometry using the anti-human CD4 (FITC)/CD69 (PE)/CD3 (PerCP) antibody staining solution (BD Biosciences) in combination with anti CD25 (APC ...
-
bioRxiv - Immunology 2021Quote: ... 4 and 6 hours and cytokines detected by intracellular cytokine staining (see Table S2 for antibodies list) using Fixation/Permeabilization Solution Kit (BD Biosciences), unstimulated cells were used as a control.
-
bioRxiv - Immunology 2021Quote: ... The cells were then washed with FACS buffer and fixed and permeabilized using Cytofix/Cytoperm Solution (BD Biosciences, San Jose, CA) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... Samples were then fixed with 1X BD FACS lysing solution before acquiring data on a FACSSymphony A5 flow cytometer (BD Biosciences).
-
bioRxiv - Immunology 2021Quote: ... Spike -specific T cells were measured by surface staining followed by fixation and permeabilization using BD fixation/permeabilization solution kit (BD Biosciences,) and intracellular cytokine staining (table S1) ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed twice in wash buffer and residual red blood cells were lysed using BD FACS Lysing Solution (BD Biosciences) for 10 minutes at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... samples were incubated for a further 15 minutes at room temperature with 500 μl of 1X BD Lysis solution (BD Biosciences) to lyse red blood cells ...
-
bioRxiv - Immunology 2022Quote: ... and cells were fixed and permeabilized at room temperature for 15 min with Fixation/Permeabilization Solution Kit (BD Biosciences; cat. #554714). For each sample a minimum of 1.2×105 stopping gate events (live CD3+ T-cells ...
-
bioRxiv - Genomics 2022Quote: ... 106 cells were resuspended in PBS supplemented with 2% fetal bovine serum and incubated with human Fc blocking solution (BD Biosciences) for 10 minutes at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... admixed with an equal number of HDFs expressing either S301D mutant or wildtype lamin A/C were re-suspended in 70 µl of Matrigel solution (BD Bioscience) and injected intradermally into the left and right side of the mouse back ...
-
bioRxiv - Bioengineering 2023Quote: ... iPSC were detached and singularised using EDTA solution (0.5 M EDTA/PBS) and replated on Matrigel (1:120, BD Bioscience, #354230) at a density of 8,000-12,000 cells/cm2 ...
-
bioRxiv - Immunology 2023Quote: ... Samples for flow cytometry were fixed in 2% paraformaldehyde solution in PBS and analyzed using a LSRII flow cytometer (BD Biosciences) and FlowJo software (Tree Star ...
-
bioRxiv - Systems Biology 2023Quote: ... A PDMS chamber was put on the mold and a solution of collagen rat tail I (5 mg/ml, BD Biosciences) was injected and allowed to polymerize at 37 °C for 1.5 hours ...
-
bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, for the RBD ELISA, and BD OptEIA Substrate Solution ...
-
bioRxiv - Cell Biology 2023Quote: ... 10-μM bromodeoxyuridine (BrdU) solution was added 4 h before the end of the experiment [fluorescein isothiocyanate (FITC) BrdU Flow Kit (BD #559619)] according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were pre-incubated for 15 minutes in a staining solution (PBS+/+, 2% FBS) containing 1 μg/mL Fc block (BD Biosciences), followed by an incubation with the antibodies for 45 minutes (Table S1) ...
-
bioRxiv - Developmental Biology 2023Quote: ... and then resuspended in 100 μL Annexin V binding buffer with either (i) FITC-Annexin V conjugated antibody (1:33 dilution in TBST) and BD propidium iodide staining solution (BD Pharmingen) or (ii ...
-
bioRxiv - Microbiology 2023Quote: ... PBMCs were then stained with live/dead and cell surface markers in MACS solution (Miltenyi) supplemented with 2% BSA (Miltenyi) prior to permeabilization with Cytofix/Cytoperm (BD Biosciences) and staining with intracellular markers in Perm/Wash (BD Biosciences) ...
-
bioRxiv - Cancer Biology 2023Quote: ... or ASC52telo cells (ATCC SCRC-4000) were suspended in 5%FBS/PBS solution and the following antibodies were added: APC-CD14 (BD 561708), APC-CD19 (BD 561742) ...
-
bioRxiv - Immunology 2023Quote: ... samples were incubated for a further 15 minutes at room temperature with 500 μl of 1X BD Lysis solution (BD Biosciences) to lyse red blood cells ...
-
bioRxiv - Microbiology 2023Quote: ... cells were incubated with fluorochrome-conjugated monoclonal antibodies (mAbs) diluted at 1:200 into a 1:3 solution of Brilliant Staining Buffer (BD 563794): Cell Staining Buffer ...
-
bioRxiv - Microbiology 2023Quote: ... Afterwards cells were washed twice with PBS and permeabilized 20 minutes with 200µl BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (BD Biosciences)at RT ...
-
bioRxiv - Immunology 2023Quote: ... solution for 20 minutes followed by 20 minutes of antibody cross linking on ice with 2 µg/mL goat-anti mouse solution (BD, #53998). T cells were then activated via an incubation period at 37 °C in FACS buffer (PBS with 2% FBS and 2 mM EDTA ...