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Citations for Becton, Dickinson and Company :
151 - 200 of 5463 citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... the bacteria Sphingomonas elodea were inoculated from the glycerol stock into a sterile Erlenmeyer flasks containing 100 mL ATCC rich broth medium (8.0 g Nutrient Broth (BD cat 234000) in 1 L deionized water) ...
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bioRxiv - Microbiology 2020Quote: ... The bacterial strains were stored in 10 % (V/V) glycerol stocks at −80 °C and freshly streaked on blood agar plates (BD Diagnostics) followed by their overnight growth at 35 °C before use ...
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bioRxiv - Developmental Biology 2019Quote: ... This strain was grown overnight from a frozen stock (25% glycerol) in 3 mL MRS medium (DifcoTM Lactobacilli MRS Broth, BD #288110) containing 10 µg/mL chloramphenicol ...
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bioRxiv - Microbiology 2023Quote: Stm strain SL1344 with plasmid pMW211[C.10E/DsRed] [20] and strain 12023 with plasmid pluxCDABE [6] were recovered from frozen glycerol stock and cultured in Difco Luria-Bertani (LB) Broth (BD Biosciences) containing 100 µg/ml ampicillin (Merck ...
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bioRxiv - Microbiology 2023Quote: ... fragilis NCTC 9343 ΔPSA44 (lacks PSA biosynthesis locus) maintained in 25% glycerol at −80 °C were thawed on Brain Heart Infusion agar plates (BHI, BD BBLTM) supplemented with 5 µg/ml hemin (Alfa Aesar ...
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bioRxiv - Immunology 2020Quote: ... Barcoded cells were then combined into a single tube prior to Fc receptor blocking (BD Biosciences) and staining with a cocktail of metal-conjugated antibodies against cell-surface markers for 30 minutes at RT with frequent gentle shaking ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific binding was blocked with FcR blocking antibody (anti-mouse CD16/32, 553142, BD Pharmingen) before surface staining with the following mAbs ...
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bioRxiv - Neuroscience 2020Quote: ... Myelin-depleted cell suspensions were incubated with an anti-CD16/CD32 Fc-blocking antibody (BD Biosciences) for 30 minutes at 4 °C to avoid Fc receptor binding and then washed once with FACS buffer ...
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bioRxiv - Immunology 2022Quote: ... the cells were stained with primary antibody in blocking solution (anti-SLP-76 pY128 from BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated in a blocking solution containing Human BD Fc Block (564220; BD Biosciences) diluted 1:25 (v/v ...
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bioRxiv - Immunology 2023Quote: ... cells were blocked using rat anti-mouse FcγIII/II receptor (CD16/CD32) blocking antibodies (BD Bioscience) for murine and Fc Receptor Binding Inhibitor Polyclonal Antibody (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-GFP (“Living Colors A.v. Peptide Antibody”) and mouse anti-UBE2E1 (BD Biosciences Inc.); rabbit-anti-γ-tubulin and mouse anti-β-actin clone AC-15 (Abcam Ltd.) ...
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bioRxiv - Immunology 2021Quote: The PBMCs were first resuspended in FACS buffer (PBS + 2% FBS) containing Fc blocking antibodies (BD, 564765) and incubated for 15 min at 4 °C ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... then incubated O/N at 4C in blocking buffer containing the mouse anti-human CD271 (BD Pharmingen) biotinylated primary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed thrice in the blocking buffer and analyzed on a LSRII flow cytometer (BD Biosciences). The percentage of cells expressing 3A-FLAG* were determined by analyzing the flow cytometry data using FlowJo software (Tree Star ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were incubated with blocking Purified Rat Anti-Mouse CD16/CD32 (553142, Mouse BD Fc Block™) for 15 min at 37°C before incubation with primary antibodies Alexa Fluor 647 Rat Anti-mouse CD31 (BD Pharmigen cat:563608 ...
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bioRxiv - Cancer Biology 2023Quote: ... Nonspecific binding of antibodies to cell Fc receptors was blocked using 10 μl FcR blocking reagent (BD Purified Rat Anti-Mouse CD16/CD32 553142 ...
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bioRxiv - Microbiology 2022Quote: Strains were streaked from -80°C freezer stocks (frozen in Brain Heart Infusion [BHI] broth with 15% glycerol) onto Brain Heart Infusion agar (BHIA; BD, Franklin Lakes, NJ), followed by incubation at 37°C for 24 h ...
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bioRxiv - Microbiology 2023Quote: Liquid cultures prepared from glycerol stocks were purified by streaking on 2X modified VL60 solidified with 14 g/L Difco noble agar (BD, Sparks, MD, USA). Nystatin (63 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... the cells were stimulated with their cognate peptide gp33 (10−6 M) and Golgistop (BD, 554724) for 5 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary incubations were completed in blocking buffer using Alexa Fluor 488 Mouse anti-SOX2 (BD Pharmingen, O30-678) and Alexa Fluor® 647 Mouse anti-CDX-2 (BD Pharmingen ...
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bioRxiv - Neuroscience 2021Quote: ... After blocking with 25 μg/ml rat anti-mouse CD16/CD32 antibody (clone 2.4G2; BD Biosciences, Heidelberg, Germany), we selected cells with Dynabeads®-conjugated antibody against mouse CD4 (L3T4 ...
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bioRxiv - Immunology 2022Quote: ... cell suspensions were first incubated with mouse CD16/CD32 Fc–blocking antibodies (clone 2.4G2, BD Biosciences Cat# 553142) and Aqua LIVE/DEAD® Fixable Dead Cell Stain (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... myeloid and hematopoietic stem and progenitor antibody panels after blocking with anti human fc block (BD Pharmingen 564219) for 10 minutes at room temperature (Supplementary Table 7 ...
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bioRxiv - Microbiology 2020Quote: ... cells are re-suspended in blocking buffer without saponine and analysed by cytofluorimetry in a FACSCalibur (BD Biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... in the presence or absence of an IFN γ blocking antibody (R4-6A2 clone, NA/LE, BD Biosciences). Cells were incubated at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... FcR blocking was performed for all samples with anti-mouse CD16/CD32 antibody (BD Biosciences, CA, San Jose). Cells were stained using the LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: The following anti-mouse antibodies were used following manufacturer’s instructions: anti-CD16/CD32 Fc blocking antibody (BD Biosciences), anti-CD24-FITC (clone M1/69 ...
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bioRxiv - Microbiology 2022Quote: ... Single cell suspensions stained in PBS + 2% FBS in the presence of Fc receptor blocking antibody (BD Pharmingen) and stained with the antibodies against the following mouse proteins ...
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bioRxiv - Molecular Biology 2021Quote: ... It was retrieved from frozen glycerol stocks and cultivated for 24 h at 28 °C on tryptic soy agar (TSA, Becton, Dickinson and Company, USA), followed by culturing in 100 mL of tryptic soy broth (TSB ...
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bioRxiv - Microbiology 2022Quote: ... a loopful from frozen glycerol stock will be aseptically transferred into 10 ml Tryptic Soy Broth plus 0.6% yeast extract (TSBYE) (Difco, Becton, Dickinson and Company, Sparks, Md.), (BeanTown Chemical) ...
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bioRxiv - Immunology 2023Quote: ... PBMC were co-cultured with peptides at 37°C for 6h with CD107a BV786 (BD Biosciences, H4A3) and 1μg/mL of CD28/CD49d costimulatory reagent (BD Biosciences) ...
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bioRxiv - Plant Biology 2023Quote: ... Bait sequences (w/o signal peptides) were fused with the GAL4 transcription factor binding domain (GAL4-BD) in the p97-BD ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in blocking buffer (PBS with 2% FBS) containing an anti-BrdU monoclonal antibody (BD Biosciences) (1:100 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... Inhibition of cell-cell junctions was achieved using a VE-cadherin function-blocking antibody (clone 75, BD Biosciences 610251) in which the cells were incubated at 12.5 μg/ml for 2 h before fixation.
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bioRxiv - Immunology 2020Quote: ... or anti-CD206 blocking antibody (10 μg/ml clone 15.2 321102, BioLegend; isotype control mouse IgG1 kappa clone 107.3; 554721, BD Biosciences) were added to cultures 60 minutes before phagocytosis assays ...
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bioRxiv - Cancer Biology 2021Quote: ... then Fc-receptors were blocked in Fc-blocking solution for 10 minutes at room temperature (BD, San Jose, CA). Sections were further blocked with with 3% goat serum in TBST for 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with a Fixable Viability Dye (eBioscience) and then with 2.4G2 antibody for FcγRII/III blocking (BD Pharmingen). Surface staining was performed at 4°C in staining buffer (PBS 1X-FCS 2%-EDTA 5mM) ...
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bioRxiv - Microbiology 2023Quote: ... BM cell suspensions (2×107/mL concentration) were treated with FcɣR-blocking agent (anti-CD16/32, clone 2.4G2, BD Biosciences) for 15 min ...
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bioRxiv - Immunology 2023Quote: ... Non-specific antibody binding was blocked by incubating the cells with Fc-blocking antibody 2.4G2 (BD, 25 μg/ml) for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: Cells were kept at 4ºC while being stained in PBS supplemented with 0.5% BSA and 2mM EDTA in the presence of antibody blocking CD16/32 (clone 2.4G2; BD 553142). All antibodies were used at a 1:200 dilution vol/vol (v/v) ...
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bioRxiv - Immunology 2019Quote: ... The MHC monomers exchanged with peptides were tetramerized in the presence of allophycocyanin (APC) conjugated streptavidin (BD Biosciences) for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... by subcutaneous injection of 200mg MOG peptide 35–55 in complete Freund’s adjuvant (Mycobacterium tuberculosis at 3.75 mg/ml; BD) followed by injection of 500ng of pertussis toxin (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... N or M peptide pools (10μg/ml) in the presence of anti-CD28 (1μg/ml; BD Biosciences, USA) and recombinant interleukin (IL)-2 (20U/ml ...
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bioRxiv - Immunology 2021Quote: Splenocytes were re-stimulated specifically for CD8+ T cells with 2 μg/mL B8R peptide (TSYKFESV, BD Pharmingen) with 5 μg/mL Brefeldin A (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 100 μl PBS/5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked with Odyssey blocking buffer and incubated with anti-LRP1 (R2629 or 5A6) or anti-SORL1 (BD Biosciences) at a concentration of 1:1000 overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were incubated for 2.5 hr with primary antibodies diluted in blocking buffer (IdU, B44, 347580; BD; CldU, ab6326, Abcam), washed several times in PBS ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... The liquid was poured off from the cells (leaving approximately 100 µl in each tube) and 5 µl of mouse Fc Blocking Reagent (rat anti-Mouse CD16/CD32 BD) was added to samples and incubated for 5 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... followed by FcR blockage with mouse serum (2%) and FcR blocking anti-mouse CD16/32 antibodies (clone 2.4G2, BD Biosciences). Next ...