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Citations for Becton, Dickinson and Company :
451 - 500 of 6767 citations for Cow Upstream stimulatory factor 1 USF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... IL-10 and IL-12p70 were quantified in those culture supernatants by ELISA with BD OptEIA™ human IL-10 and IL-12p70 sets following the manufacturer's instructions (BD Bioscience).
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bioRxiv - Immunology 2020Quote: ... Interleukin-6 (IL-6) expression was measured by ELISA in SF-supernatants according to the manufacturer’s instructions (BD Biosciences, Oxford, UK).
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bioRxiv - Immunology 2023Quote: ... IL- 10 secretion was determined by ELISA in supernatants harvested after a 24 hour restimulation with anti-CD3 (555336, BD Biosciences) and anti-CD28 (555725 ...
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bioRxiv - Neuroscience 2023Quote: Cytokine levels in clarified tissue lung homogenates were determined by enzyme linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (BD Biosciences, USA) and read with CLARIOstar Plus (BMG labtech ...
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bioRxiv - Immunology 2023Quote: ... including analysis of activation markers by flow cytometry and IL-12(p70) and IL-10 secretion by ELISA (555183 and 555157, BD Biosciences). For this ...
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bioRxiv - Microbiology 2024Quote: ... The antibodies employed for ELISA were biotin-conjugated rat anti-mouse IgG2a (clone R19-5, BD Biosciences, Denmark cat no. 550332) and anti-mouse IgG conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... IPF-HLFs or nHLFs were cultured in 24-well plates previously coated with 10 mg/mL growth factor-reduced (GFR) Matrigel (BD Biosciences, San Jose, California). Following 48 h ...
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bioRxiv - Biochemistry 2023Quote: Invasion assays were done in 8 μm pore size of the Chemotaxicell chamber coated with 100 μg/ml Growth factor reduced Matrigel (BD Biosciences, San Jose CA) for 3 h at 37°C and blocked with 0.1% bovine serum albumin in PBS ...
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bioRxiv - Microbiology 2021Quote: ... intracellular HIV-1-p24 staining was performed using the Cytofix/CytopermTM kit (BD #554714) using anti-HIV-1 p24-PE antibody (#6604667 ...
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bioRxiv - Cell Biology 2020Quote: ... and macrophage chemoattractant protein-1 (MCP-1) were quantified by bead-based flow cytometry assay (CBA Kit; BD Biosciences, Heidelberg, Germany) in accordance with the instructions of the manufacturer.
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bioRxiv - Immunology 2021Quote: ... Flow cytometry was conducted and the supernatant was collected from each well to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... and the supernatants were harvested for cytokine detection (IL-10 and TGF-β) by specific ELISAs using a standard protocol (BD OptEIA). The detection limits for IL-10 is 31.3–2000 pg/ml e TGF- β is 62.5–4,000 pg/mL) ...
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bioRxiv - Immunology 2020Quote: ... IL-2 and IFN-γ concentration secreted in cell supernatant were measured using sandwich ELISA according to manufacturer’s protocol (BD Biosciences, USA). Plates were read on a Varioskan Lux multimode microplate reader (ThermoFisher ...
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bioRxiv - Neuroscience 2019Quote: ... mIL-6 levels were evaluated using sandwich capture ELISA assays using the RIPA soluble mouse forebrain lysates as per manufacturer’s instructions (BD OptiEIA, BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected after 6 days of co-culture to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
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bioRxiv - Immunology 2022Quote: Secretion of CXCL10 (IP-10) into the medium was quantified 24 h after stimulation by ELISA (Human IP-10; BD OptEIA, 550926). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Concentrations of IL-1β and IL-6 were assayed according to protocol using commercially available mouse IL-1β and IL-6 ELISA (BD Bioscience, Australia). UV absorbance was quantified on a BMG PolarStar microplate reader (BMG Labtechnologies ...
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bioRxiv - Cancer Biology 2023Quote: ... IFN-γ and TGF-β in mouse serum was performed with the BD OptEIATM Mouse IL-10 ELISA Set (BD Biosciences, 555252), BD OptEIATM Mouse TNF (Mono/Mono ...
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bioRxiv - Neuroscience 2021Quote: ... were co-cultured with murine hypoxia-inducible factor knock out (HIFko) astrocytes (passage 12, received from Le Joncour) on hanging cell culture inserts (BD Falcon 353091, Franklin Lakes, NJ) for 5 days in fetal bovine serum (FBS)-free conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... The final pellet containing organoids was re-suspended in Matrigel and distributed in 96-well culture plates containing an underlay Matrigel layer (Growth Factor Reduced Matrigel, BD Biosciences, San Jose, CA, USA). Cultures were maintained at 37 °C in serum-free basal medium (DMEF/F12 ...
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bioRxiv - Genomics 2021Quote: Supernatants from stimulated J774 macrophages (above) were analyzed for cytokine IL12p40 production through ELISA following manufacturer’s protocol (BD Bioscience Opt EIA, catalog #555165). They were assayed in 4 independent experiments with 2-3 technical replicate wells.
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bioRxiv - Neuroscience 2022Quote: ... Samples are ultracentrifuged and pellets were resuspended and incubated onto 96 well ELISA plates containing 5ug/mL anti-syn42 monoclonal antibody (BD transduction #610-787). ELISAs were carried out on test samples run alongside serial diluted standard synthetic α-Syn ...
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bioRxiv - Cell Biology 2023Quote: ... and interleukin-2 cytokine production was measured using BD optiEA Mouse IL-2 ELISA set following the manufacturer’s instructions (BD Biosciences, Cat no. 555148).
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bioRxiv - Microbiology 2023Quote: ... and IL-18 were measured in mouse colonic tissue extracts by using the BD OptEIA ELISA System (BD Biosciences, San Jose, CA, USA). All assays were performed as per the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... brefeldin A (1.5 μg/ml) and monensin (1 μM) using CytoFix/CytoPerm kit (BD Bioscience). Likewise ...
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bioRxiv - Immunology 2020Quote: IgG subclasses were measured from day 21 sera using the same ELISA protocol with the following secondary Abs: goat anti-mouse IgG1-HRP (BD Biosciences, San Jose, California), goat anti-mouse IgG2b-HRP (1:1000 ...
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bioRxiv - Immunology 2022Quote: The levels of cytokines in cell culture medium were determined with BD OptEIA™ Mouse IL-6 ELISA set (BD Biosciences, Franklin Lakes, NJ), ELISA MAX™ Standard Set Mouse IL-10 (BioLegend) ...
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bioRxiv - Microbiology 2023Quote: ... The cytokine releases were evaluated by ELISA in cell supernatants or in peritoneal fluids (IL-1β, IL-12, IL-6, TNFα, TGFβ, IL-10 BD Biosciences, R&D Systems). 8 isoprostane ...
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bioRxiv - Cell Biology 2024Quote: ... CCL2/MCP-1 and CXCL10/IP-10 were quantified using CBA human chemokine kit (BD Biosciences), according to the instructions from the manufacturer ...
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bioRxiv - Immunology 2019Quote: ... Serum was collected by tail-tipping before LPS injection and 2 and 4 hours following injection for IL6 determination by ELISA (BD; OptEIATM Set Mouse IL-6), as per manufactures instructions.
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bioRxiv - Microbiology 2020Quote: ... and mIL-2 was measured by ELISA using the capture Ab JES6-1A12 and the biotinylated detection Ab JES6-5H4 at 450 nm (BD Pharmingen™; San Diego, USA). Experiments were performed in triplicate.
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bioRxiv - Molecular Biology 2020Quote: ... TNF-α and MCP-1 were analyzed by bead-based flow cytometry assay (CBA kit, BD Biosciences), TGF-β and VEGF-A with specific ELISA (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: ... 1×105 cells were stained using PE Annexin V Apoptosis Detection Kit I (BD Biosciences, Cat # 559763) following to the manufacture’s protocol ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... HE2 and HP populations were FACS sorted after incubation with KIT-APC (1:100)(BD Pharmingen, 553356), TIE2-PE (1:200)(eBioscience ...
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bioRxiv - Immunology 2022Quote: ... The c-kit− fraction was stained with antibodies against CD11C (APC, clone HC3, BD biosciences, 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Immunology 2023Quote: ... modifications to the protocol were made: 1 μl of 2 μM additive primer (BD Genomics, beta kit) specific to the antibodies tags was added to the amplification mixture ...
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bioRxiv - Immunology 2021Quote: ... and anti-inflammatory cytokines (IL-10, TGF-β) were determined in plasma by ELISA at the indicated time points (OptEIA set; BD Biosciences, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Mitochondrial membrane potential was checked by JC-1 FACS analysis (BD Mitoscreen Mitochondrial Membrane Potential Detection Kit, BD Biosciences). Treated and non-treated cells were incubated with JC-1 for 15-20 minutes ...
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bioRxiv - Immunology 2022Quote: ... MCP-1 and IL-6 cytokine levels were quantified using the Cytometric bead assay Mouse inflammation kit (BD Bioscience) as recommended by the manufacturer and measured by flow cytometry using a FACS Fortessa flow cytometer.
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bioRxiv - Neuroscience 2023Quote: ... and performed reverse transcription and an exonuclease reaction using the version 1 3’ kit (BD Biosciences, cat. 633733, 633773).
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bioRxiv - Immunology 2021Quote: ... co-culture supernatants were thawed at room temperature for 1 hr and cytokines stained with mouse Th1/Th2/Th17 cytokine kit (BD) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... the apoptosis of PANC-1 cells was determined by using the Annexin V-FITC/PI apoptosis detection kit (BD Biosciences). The cells were harvested with trypsin-EDTA (Gibco ...
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bioRxiv - Immunology 2021Quote: ... lung single cell suspensions were stained with tetramer prior to the surface and intracellular staining for 1 hour at 37°C.Intracellular cytokine staining was performed using the BD Cytofix/Cytoperm kit (BD Biosciences) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... buffer (PBS containing 0.1% sodium azide and 1% bovine serum albumin) and fixed/permeabilized using a Cytofix/Cytoperm Buffer Kit (BD Biosciences) or a FoxP3 Transcription Factor Buffer Kit (eBioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was collected to determine the levels of MCP-1/CCL2 using commercial kit (BD Biosciences, CA, USA, Cat# 555179) as per manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Amplification kit and BD Single-Cell Multiplexing kit (BD Biosciences). Size-distribution (Quality ...
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bioRxiv - Microbiology 2021Quote: ... a BrdU assay kit (APC BrdU flow kit, BD Pharmingen) was used as directed by the manufacturer’s instruction ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... and with hematopoietic stem cell markers APC-conjugated anti-CD117 (c-Kit) and PE-conjugated Ly6A/E (Sca-1) (BD Biosciences). Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies ...
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bioRxiv - Immunology 2019Quote: ... cells were cultured in the presence of 1 μg/mL Brefeldin A for indicated timepoints and were fixed and permeabilized with Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... We then double sorted lineage depleted cells for an HSC-enriched population (Lin- Sca-1+c-Kit+CD34+-CD135-) into 2% FBS in HBSS using fluorescent activated cell sorting (FACS) (BD Aria). Recipient mice ...