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Citations for Becton, Dickinson and Company :
351 - 400 of 6962 citations for Cow Polypyrimidine tract binding protein 1 PTBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A, 1 µg/mL and BD Golgi Stop™ containing monensin ...
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bioRxiv - Immunology 2021Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A, 1 µg/mL and BD Golgi Stop™ containing monensin ...
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bioRxiv - Immunology 2023Quote: ... and 1 µL/mL GolgiPlug™ Protein Transport Inhibitor (containing Brefeldin A, BD Biosciences, #555029). Cell suspensions were washed between each step with sterile PBS containing 2% FBS (FACS buffer).
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bioRxiv - Pharmacology and Toxicology 2019Quote: 344SQ murine lung adenocarcinoma cells expressing firefly luciferase and green fluorescent protein (in 50 μL of 1:1 mix of HBSS and BD Matrigel) were injected into the left lung of 8 weeks old female 129/Sv mice via intrapulmonary injection as described previously (50) ...
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bioRxiv - Neuroscience 2022Quote: ... that was blocked in 5% non-fat milk for 1 h at room temperature and incubated overnight with primary antibodies against the following proteins: α-synuclein (1:1000, BD), NCK2 (1:1000 ...
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bioRxiv - Immunology 2019Quote: A375 cell production of chemokine proteins was quantified using a CBA Human Chemokine Kit (BD Biosciences Cat #552990) and GM-CSF production was measured using Human GM-CSF ELISA Kit (Abcam Cat #ab174448) ...
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bioRxiv - Microbiology 2021Quote: ... creating either N- or C-terminally tagged GAL4 activation domain (AD/prey) or GAL4 DNA-binding domain (BD/bait) fusion proteins ...
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bioRxiv - Molecular Biology 2021Quote: The TaAFR CDS was cloned into the yeast bait vector pGBKT7 which carries the GAL4 DNA-binding domain (BD), and the construct ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells from mouse tissues were incubated with nonspecific binding blocker (anti-mouse/human CD16/CD32 Fcγ receptor; BD Biosciences) before staining for V450-human CD45 (BD Biosciences # 560368) ...
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bioRxiv - Immunology 2021Quote: ... 100μL of annexin V binding buffer was added and cells were immediately analyzed on an Accuri C6 (BD Biosciences). Phagocytosis of pHrodo Red S ...
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bioRxiv - Developmental Biology 2021Quote: ... 2014) by fusing each as a bait to the DNA-binding domain of the GAL4 transcription factor (GAL4-BD) in plasmid pGBKT7 (Clontech) ...
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bioRxiv - Cancer Biology 2020Quote: ... 5.105 cells were washed with PBS and resuspended in 200 μL of Annexin-V binding buffer (BD, Cat# 556420). Two microliters of Annexin-V-FITC (BD ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells from mouse tissues were incubated with nonspecific binding blocker (anti-mouse/human CD16/CD32 Fcy receptor, BD Biosciences) before staining for APC-human CD33 (Biolegend #303408) ...
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bioRxiv - Cancer Biology 2022Quote: ... 2×105 cells were washed with PBS1X and resuspended in 100 µL of Annexin-V binding buffer (BD Biosciences). 1 µL of Annexin-V-APC (Cat# 550474) ...
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A Phytochrome B-PIF4-MYC2 Module Tunes Secondary Cell Wall Thickening in Response to Shaded LightingbioRxiv - Plant Biology 2021Quote: The coding sequence of MYC2 PCR-amplified from cDNA using PHANTA was fused with GAL4 DNA-binding domain (BD) of the bait vector pGBKT7 (Clontech) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were detached using TrypLE and resuspended in 1 mL of binding buffer (HEPES-buffered PBS supplemented with 2.5 mM calcium chloride) containing anti-annexin V-APC (BD) and incubated for 15 min in the dark at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Live tetramer-binding cells were sorted the basis of co-receptor expression using an Influx flow cytometer (BD Biosciences), rested overnight in RPMI 1640 supplemented with 10% heat-inactivated pooled human serum and 0.5ng/ml rhIL-2 ...
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bioRxiv - Microbiology 2020Quote: ... the different truncation/deletion mutants of CVB3 VP1 were cloned into pGBKT7 vector (between the Nde I & Pst I sites) in frame with the vector’s GAL4 DNA Binding Domain (BD), and MAT1 was expressed in the pGADT7 vector (between the EcoR I & BamH I sites ...
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bioRxiv - Microbiology 2021Quote: ... 400 μL of binding buffer was added to the solution and further analyzed by FACScallibur flow cytometer (BD Biosciences).
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bioRxiv - Cell Biology 2020Quote: ... cells were suspended in 200μl of binding buffer and immediately proceed using an LSR II flow cytometer (BD Biosciences) and analyzed using FlowJo software (FlowJo LLC ...
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bioRxiv - Immunology 2023Quote: ... 4°C for 10 min and binding was analyzed by flow cytometer FACS LSRFortessaTM (BD Bioscience, Franklin Lakes, NJ) using Yellow/Green Ex 561 nm Em 586/15 laser for the detection of antibodies ...
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bioRxiv - Immunology 2023Quote: ... Non-specific antibody binding was blocked by incubating the cells with Fc-blocking antibody 2.4G2 (BD, 25 μg/ml) for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... and blocked unspecific bindings with using anti-mouse CD16/CD32 (Mouse BD Fc Block, BD Biosciences, Franklin Lakes, NJ). Cells were then stained with FITC-conjugated anti-mouse CD45 (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... IL-17 and IL-10 by ELISA following the manufacturer instructions (BD Biosciences, San Jose, CA).
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bioRxiv - Immunology 2021Quote: ... Levels of IFNγ were measured by ELISA following the manufacturer’s protocol (BD, OptEIA™ Human IFNγ).
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bioRxiv - Immunology 2023Quote: ... IP-10 in tissue culture supernatants was quantified with a Human IP-10 ELISA Set (BD).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The strep avidin horse radish peroxidase used in the TNF-α ELISA was purchased from BD Biosciences (NSW ...
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bioRxiv - Neuroscience 2023Quote: ... astrocytes (1×106) were incubated with protein transport inhibitor containing Brefeldin A (555029 Golgiplug, BD Biosciences) and Monensin (554724 Golgistop ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% penicillin/streptomycin and 10 mM HEPES [pH7.4]) with 25% high protein Matrigel (354248; BD Biosciences). PDX single cell suspensions were obtained by mechanically dissociated (12-141-363 ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-KIT rat monoclonal antibody (BD Biosciences, 553352, 1:200), anti-DDX4 rabbit polyclonal antibody (abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were resuspended in 200 μL 1x binding buffer with or without 5 μL Annexin V (BD Biosciences, Billerica, MA). After 15 min incubation ...
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bioRxiv - Plant Biology 2020Quote: ... 10 μL of 10-fold serial dilutions of yeast harbouring an activation domain (AD)-AtCBLs or empty vector pTOOL28 and either DNA-binding domain (BD)-AtCIPK16 or empty vector pTOOL27 were spotted on plates containing DDO (SD/-Leu/-Trp ...
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bioRxiv - Developmental Biology 2021Quote: ... and another one bearing amino acids 329–542 were cloned into the pOAD and or pOBD2 vectors (Cagney et al., 2000) in-frame either with the activator domain (AD) or the DNA-binding domain (BD) sequence of GAL4 ...
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bioRxiv - Pathology 2019Quote: ... and V2C84AC86A were cloned into the activation domain (AD)-containing vector and SlSGS3 was cloned into the vector harboring the DNA binding domain (BD). Both constructs were transformed into Saccharomyces cerevisiae strain AH109 ...
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bioRxiv - Immunology 2019Quote: ... were labeled with a cocktail of non-APC binding antibodies namely the murine T-cell lineage directed anti-Thy-1.2 antibody (BD Pharmingen) and the T-cell subsets recognizing CD4 antibody (eBioscience ...
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bioRxiv - Biochemistry 2021Quote: ... The expression and binding signals were determined by flow cytometry using BD Accuri™ C6 Flow Cytometer (BD Biosciences, USA). The cell analysis and sorting were done by S3e Cell Sorter (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... coding sequence were inserted into pGADC1 vector harbouring GAL4 activation domain (AD) and pBDUC1 vector harbouring GAL4 DNA binding domain (BD).
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bioRxiv - Neuroscience 2020Quote: The vector used to insert the bait cDNAs in the yeast two-hybrid (YTH) screens was Gal4 DNA binding domain (Gal4-BD) expression vector pAS2-1 (Clontech ...
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bioRxiv - Microbiology 2020Quote: ... Gene sequences without predicted introns were inserted into the vectors pGAD24 and pGBKT7 generating translational fusions to the Gal4 activation domain (AD) and DNA-binding domain (BD), respectively.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in 200 uL of binding buffer after discarding the supernatant and analyzed via flow cytometry using a FACSCalibur Flow Cytometer (BD).
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bioRxiv - Molecular Biology 2020Quote: ... CDS (pENTR/D-TOPO) were sub-cloned into both pGADT7-GW (activation domain, AD) and pGBKT7-GW (binding domain, BD) to generate SWCDS-AD and SWCDS-BD ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in 200 uL of binding buffer after discarding the supernatant and analyzed via flow cytometry using a FACSCalibur Flow Cytometer (BD).
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bioRxiv - Cancer Biology 2019Quote: ... and resuspended in Annexin V binding buffer (10 mM HEPES 7.4, 140 mM NaCl, 2.5 mM CaCl2) with Annexin-V FITC (BD Biosciences 556419). Flow cytometry was performed on a Becton Dickinson LSRFortessa or LSRII flow cytometer ...
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bioRxiv - Microbiology 2020Quote: ... cells were resuspended and 2-43 binding to cell-surface S trimer was quantified on LSRII flow cytometer (BD Biosciences). The mean fluorescence intensity of APC in GFP-positive cells was analyzed using FlowJo and the relative binding of 2-43 to S trimer in the presence of competitors was calculated as the percentage of the mean fluorescence intensity compared to that of the competitor-free controls.
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bioRxiv - Microbiology 2021Quote: ... from pDONR207 into the yeast two-hybrid vector pPC97-GW for expression in fusion downstream of the GAL4 DNA-binding domain (GAL4-BD). The control siRNA and a pool of TAX1BP1 siRNA (Ambion ...
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bioRxiv - Genetics 2020Quote: ... Both WT and TG8B RTY were transferred to pACT-GW (GAL4-Activation Domain, AD) and pAS-GW (GAL4-Binding Domain, BD) (Nakagawa et al. ...
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bioRxiv - Cell Biology 2022Quote: ... was then transferred to a 96-well plate and fALOD4-Neon binding was measured by analyzing 10,000 RBCs for each condition on a Stratedigm S1000 EX Flow Cytometer (BD Biosciences). Fluorescently labeled RBCs were analyzed using FlowJo software version 10 (Ashland ...
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bioRxiv - Plant Biology 2022Quote: ... while the full-length coding sequence of HlMYB7 was cloned into the prey vector pGADT7-GW [DNA-binding domain (BD)] (Addgene Inc ...
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bioRxiv - Plant Biology 2022Quote: ... and the PCR products were fused into the activation domain (AD) vector pGADT7 and/or the DNA-binding domain (BD) vector pGBKT7 and verified by sequencing ...
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bioRxiv - Immunology 2022Quote: ... the amplified PGRP3 cDNA was digested with BamHI and EcoRI and ligated to a pGBKT7 vector containing the GAL4 DNA-binding domain (BD). Successful recombinant pGBKT7-PGRP3 bait construction was verified with restriction digestion (BamHI ...