Labshake search
Citations for Becton, Dickinson and Company :
201 - 250 of 3906 citations for Aprataxin And PNKP Like Factor APLF Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... generated EBs were collected and embedded in 50μl (around 40 EBs) of growth-factor-reduced Matrigel® (BD Biosciences), plated in 12-well plates and treated for three days with 1 mg/ml Doxycycline (Sigma) ...
-
bioRxiv - Cell Biology 2020Quote: Human iPSCs were cultured in mTeSR®1 medium (STEMCELL) on growth factor-reduced Matrigel™ Matrix (BD Biosciences)-coated plates at 37°C in a 5% CO2 incubator ...
-
bioRxiv - Cancer Biology 2022Quote: ... the transwell inserts from the CIM plates were coated with 20 μl of growth-factor-reduced Matrigel (BD Biosciences), at a concentration of 250 μg/ml ...
-
bioRxiv - Cancer Biology 2019Quote: ... Intracellular cytokine and transcription factor staining were performed right before analysis using either the BD Perm/Wash Buffer (BD) or the Foxp3 Transcription Factor Staining Buffer Kit (eBioscience) ...
-
bioRxiv - Developmental Biology 2021Quote: ... from E16.5 C57BL/6 lungs pooled by litter and cultured on a layer of growth factor reduced Matrigel (BD) in serum-free media (DMEM/F12 supplemented with L-glutamine and penicillin-streptomycin ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tumour xenografts were generated by injection of 1 x 106 cells in 50% growth factor reduced Matrigel (BD Biosciences) into the flanks of CBA.Nude mice ...
-
bioRxiv - Immunology 2023Quote: ... samples were washed two times with 1X permeabilization and wash buffer from the transcription factor buffer kit (BD Biosciences) and stained with antibodies against IFN-γ ...
-
bioRxiv - Cell Biology 2023Quote: ... the following were also supplemented: 20 ng/mL brain-derived neurotrophic factor (BDNF; R&D systems, catalog #: 248-BD), 20 ng/mL glial cell line-derived neurotrophic factor (GDNF ...
-
bioRxiv - Developmental Biology 2022Quote: ... A density of 100,000 cells per well was used for culture in 8-well #1 coverglass chambers (Labtek) precoated with growth factor reduced Matrigel (80µl, BD Biosciences) for 30 minutes at 37°C ...
-
bioRxiv - Cancer Biology 2021Quote: 1.5×106 pCAF2s expressing shCtl or shPYCR1 and 5×105 MCF10DCIS.com in 200 µl 50% growth factor reduced phenol red free Matrigel (BD Biosciences) in PBS were injected subcutaneously into the flank of 8 week old female BALB/c nude mice (Charles River) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1.5×106 pCAF2 and 5×105 MCF10DCIS.com in 200 µl 50% growth factor reduced phenol red free Matrigel (BD Biosciences) in PBS were injected subcutaneously into the flank of 8 week old female BALB/c nude mice (Charles River) ...
-
bioRxiv - Cancer Biology 2019Quote: The tube formation assay was performed on a 48-well tissue culture dish that was coated with 150 µl growth factor-reduced Matrigel (BD) that was left to solidify at 37°C for 30 min ...
-
bioRxiv - Cancer Biology 2021Quote: ... LLC-CC and LLC-PON1 cell suspensions (1.2×107 cells) in 200 μL of culture medium/growth factor-reduced Matrigel (BD Biosciences) in a 1:1 ratio were subcutaneously injected into the right flank of each mouse ...
-
bioRxiv - Microbiology 2020Quote: ... Approximately 500 crypts were suspended in 50 μL of growth factor-reduced phenol-free Matrigel (BD Biosciences, San Jose, CA). Next ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1,000 cells were cultured during the length of the experiment in 100 μl basement membrane gels composed of 9.2 μg/μl phenol red-free growth factor reduced Matrigel (BD Bioscience) in 96 well plates ...
-
bioRxiv - Immunology 2022Quote: ... Cells were resuspended in a suitable amount of PBS and mixed at a 1:1 (v:v) ratio with growth factor reduced Matrigel Matrix (BD Biosciences). A final injection volume of 100ul containing 400k cells was injected subcutaneously on the flanks of mice.
-
bioRxiv - Immunology 2022Quote: Intracellular staining for MX-1 was performed for 1 × 105 thawed PBMC using the Transcription Factor Buffer Set (BD Biosciences) according to the manufacturer’s directions ...
-
bioRxiv - Developmental Biology 2021Quote: ... generated by hanging drops culturing of ESCs (1,000 cells per drop) were collected after 4 days and embedded in growth-factor-restricted Matrigel (MTG; BD Biosciences); 50µl MTG drops (containing around 20 EBs ...
-
bioRxiv - Cancer Biology 2020Quote: Sorted tumor cells were seeded at a density of 45 cells per 96/well on Growth factor-reduced Matrigel (BD) and allowed to grow for up to 6 days under standard culture conditions (See cell culture) ...
-
bioRxiv - Cancer Biology 2021Quote: ... HCC1806 cells were seeded at a density of 15,000 cells per well in a 48-well tissue culture plate coated with 125μl of Matrigel Growth Factor Reduced (BD Biosciences). Media was replaced every 72h and growth was monitored for 9 days ...
-
bioRxiv - Cell Biology 2021Quote: ... iPSCs were seeded at 1.25-2.5×104 cells/cm2 onto 12-well plates coated with 80 μg/μl growth factor-reduced Matrigel (BD Biosciences) in mTeSR1 supplemented with 10 μM Y-27632 (Millipore ...
-
bioRxiv - Cancer Biology 2020Quote: ... single cell suspensions were made (1.5 x 104 cells per ml) in the appropriate medium supplemented with 2% Growth Factor Reduced Matrigel (GFRM; BD Biosciences). 150μl of this mix was plated per well in a 96 well ImageLock plate (Essen Biosciences ...
-
bioRxiv - Genetics 2022Quote: ... a model in which liability to externalizing is captured by a general factor onto which all phenotypes load and residual liability to behavioral disinhibition (BD) and substance use (SUB ...
-
bioRxiv - Microbiology 2022Quote: ... leading to upregulation of virulence factors expression [11] and the three clinical isolates were cultivated in Todd Hewitt Broth (THB; BD) supplemented with 2% yeast extract (THY ...
-
bioRxiv - Immunology 2023Quote: ... Intracellular expression of cytokines and transcription factors was analyzed using the respective fluorochrome-coupled mAbs in conjunction with either the BD Cytofix/Cytoperm kit (BD) or the Foxp3 staining buffer set (eBioscience ...
-
bioRxiv - Immunology 2023Quote: ... Intracellular staining for cytoplasmic and nuclear proteins were performed with Transcription Factor Staining Buffer kit according to manufacturer’s instructions (BD Pharmingen). Dead cells were excluded by DAPI (BioLegend ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were incubated at 37°C for 4 hours after which they were fixed and permeabilized with the Transcription Factor Buffer Set from BD. γH2AX was detected with PerCP/Cyanine5.5 anti-H2A.X-Phosphorylated (Ser139 ...
-
bioRxiv - Cancer Biology 2021Quote: ... single cell suspensions (1.5 x 104 cells per ml) were plated in the appropriate medium supplemented with 2% Growth Factor Reduced Matrigel (GFRM; BD Biosciences). 100μl of this mix were added per well in a 96 well ImageLock plate (Essen Biosciences ...
-
bioRxiv - Cell Biology 2020Quote: ... or cell carrier 96 wells plates were coated with a thick layer (150-200uL/cm2) of a mix of growth factor-reduced (GFR) Matrigel (BD Biosciences) and neutralized Bovine Collagen I (Purecol ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells (500-700) were seeded into a 500 μL layer of a 1:1 collagen/Growth Factor Reduced MatrigelTM (#354230, Corning/BD Biosciences) mixed in DMEM balance with bicarbonate and were treated twice a week for one month ...
-
bioRxiv - Cell Biology 2019Quote: ... macrophage inflammatory protein (MIP-1α) and tumor necrosis factor (TNF-α) were detected using Cytometric Bead Array (CBA) technology (BD Biosciences) and assayed on an Accuri C6 flow cytometer (BD Biosciences ...
-
bioRxiv - Microbiology 2021Quote: ... 25 μg/ml gentamicin and 100 U/ml penicillin/streptomycin) and were then added to 150 μl of BD Growth Factor Reduced Matrigel Matrix (356230; BD Biosciences). Fifty microliter droplets were added to consecutive wells of a 24-well tissue culture plate (3524 ...
-
bioRxiv - Immunology 2021Quote: ... cells were initially fixed and permeabilized for 1 hour using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) and then stained using the PE-conjugated anti-p53 Set (BD Biosciences), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were plated at a density of 1.0 × 104 cells per well in triplicate in 96-well plates coated with 50 μl of growth factor - reduced Matrigel (BD Biosciences). Plates were incubated overnight at 37° C ...
-
bioRxiv - Bioengineering 2022Quote: ... 2×105 cells were plated in a well of a 12-well plate coated with 80 μg/ml Growth Factor Reduced Matrigel Basement Membrane Matrix (BD Biosciences). One day later ...
-
bioRxiv - Cancer Biology 2021Quote: ... MCF-10A and MCF-10A MYC-ER cells were seeded at a density of 10,000 cells per well in triplicate on a 4-well glass chamber slide coated with Matrigel Growth Factor Reduced (BD Biosciences) as described (Debnath et al. ...
-
bioRxiv - Immunology 2022Quote: ... The Foxp3 staining kit was used for intracellular transcription factor staining and the Cytoperm/Cytofix Plus staining kit was used for cytokine staining (BD Biosciences). Data was analyzed using FlowJo (TreeStar) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Collected tissue fragments were counted and plated at a density of 1000 cell fragments per 50 μL of Growth Factor Reduced Matrigel (BD Biosciences) in 24 well plates (Nunc) ...
-
bioRxiv - Neuroscience 2019Quote: ... and a total of 5×105 (50μl) Ret-mCherry sorted (RMS) cells in a 1:1 suspension of PBS with growth factor–reduced Matrigel (356231, BD Biosciences) were injected subdermally to the right dorsal side ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were plated at a density of 1.5×106 cells/well on 18mm coverslips (Neuvitro 18-GG-PDL) precoated with high concentration growth factor-reduced Matrigel (BDBiosciences, 354263) and cultured at 37°C ...
-
bioRxiv - Bioengineering 2021Quote: ... for assays which required non-transcription factor internal staining (cytokines alone) fixation and permeabilization was performed using the Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer instructions ...
-
bioRxiv - Immunology 2021Quote: ... Cells were fixed and permeabilized for intracellular staining with the FoxP3/Transcription Factor Staining Buffer Set (eBioscience) prior to staining with FoxP3-Alexa Fluor 488 (259D, BD Biosciences) (Table E2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and NLF-shNT or -shPRELP (0.5×106 cells) were suspended in 20 µl of cell culture medium and growth-factor-reduced Matrigel (catalog no. 356230; BD Biosciences) 1:1 and co-injected into the liver of six-week old athymic nude mice (n=5 per condition).
-
bioRxiv - Cancer Biology 2021Quote: ... or 1 × 106 SK-Mel-24 cells and 1 × 106 uninduced GFP/M27 or bcat-GFP/M27 in 50 µl of growth-factor-reduced Matrigel/PBS (1:1) (BD Biosciences) was injected intradermally into the flanks of 4-6-week-old NOD.Cg-Prkdcscid/J mice (Jackson Laboratory ...
-
bioRxiv - Immunology 2021Quote: ... Intracellular stains were performed using anti-T-beteFluor66O (clone 4B10; eBioscience) in conjunction with a Transcription Factor Buffer Set (BD Biosciences). Samples were acquired using an LSR Fortessa or a FACSCanto II (BD Biosciences) ...
-
bioRxiv - Cancer Biology 2022Quote: Cell migration and invasion were performed by using BioCoatTM Control Cell Culture Insertsin and BioCoatTM Growth Factor Reduced Matrigel Invasion Chambers (BD Biosciences), respectively ...
-
bioRxiv - Immunology 2022Quote: ... cells were stained for surface antigens as above and then fixed and permeabilised with the Transcription Factor buffer set (562725, BD Biosciences). Next cells were incubated with 4M HCL for 10 min at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... The resulting pellet was resuspended in 1 ml of complete neurosphere medium containing epidermal growth factor (EGF, 20 ng/ml; receptor grade, BD Biosciences) and basic fibroblast growth factor (bFGF ...
-
bioRxiv - Neuroscience 2022Quote: ... and tumor necrosis factor alpha (TNFα) in cell supernatants were measured using the Human Inflammatory Cytokine Cytometric Bead Array Kit (BD Biosciences). A LEGENDplex™ HSC Myeloid Panel kit (Biolegend ...
-
bioRxiv - Molecular Biology 2022Quote: ... Confluent definitive endoderm cells are then passed 1:3 in presence of Rock Inhibitor on growth factors reduced Matrigel (BD Biosciences) and cultured for 8 days in differentiation medium (DMEM F12 ...