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Citations for Becton, Dickinson and Company :
351 - 400 of 3905 citations for ATPase H+ Transporting V0 Subunit A1 ATP6V0A1 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Cells were incubated at 37°C with 5% CO2 for 24 h and then sorted for GFP+ expression using fluorescent activated cell sorting (FACS) on a FACSAria II (BD Bioscience). The GFP+ pool (bulk ...
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bioRxiv - Immunology 2022Quote: ... cells were stimulated for 4 h with eBioscience™ Cell Stimulation Cocktail and a protein transport inhibitor-containing Brefeldin (1.5uL/mL StopGolgi; BD Biosciences). Cells were then washed in FACS buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were blocked for 1 h in 5% milk/TBS Tween-20 solution and incubated for 1 h with anti-TOP2β (1:5,000, mouse anti-human, Clone 40/Topo IIβ, RUO – 611492, BD Biosciences-US) or anti-TOP2α (1:10,000 ...
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bioRxiv - Immunology 2021Quote: ... the killing capacity of NK cells was tested when they coculture with senescent / young or modulated for ST8SIA1 expression MRC5.NK cells are added to the culture for 4 h in presence of monensin and brefeldin (BD Biosciences) at the effector/target ratio of 1:1 ...
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bioRxiv - Bioengineering 2021Quote: ... H-2Kb-negative GFP-positive cells were sorted into single cells on a 96-well plate using FACSAria Fusion (BD Biosciences) and cultured for 2-3 weeks in D10 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected at 72 h post FeNPs administration and detected with the Annexin V-FITC Apoptosis Detection Kit (BD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The percentage of GFP-positive cells among the mCherry-positive cells was determined 48 h after I-SceI transfection using a LSRFortessa X-20 (BD Bioscience) using BD FACSDiva Software v8.0.1 ...
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bioRxiv - Microbiology 2020Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT were followed by a 1 h incubation with biotinylated goat anti-rat (559286, BD biosciences) 1:100 in TNB ...
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bioRxiv - Bioengineering 2022Quote: ... Genes encoding the proteins were cloned into pET29b between the restriction sites NdeI and XhoI and expressed for 4 h in Terrific Broth (BD Difco) using Lemo21 (NEB ...
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bioRxiv - Microbiology 2022Quote: ... aureus was also grown in cell free supernatants (CFS) of Fusobacterium nucleatum ATCC 25586 that had been cultured anaerobically for 48 h in BBL Brucella Broth (BD 2011088) supplemented with 250 μg/mL and 50 μg/mL of hemin and vitamin K (Hardy Diagnostics Z237) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were allowed to activate for 24 and 48 h and then stained with Annexin V FITC and 7AAD (BD Pharmingen). Cell proliferation was analyzed 96 hours post polyclonal activation in CD4+ T cells previously stained with cell trace violet dye (Thermo Fisher).
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bioRxiv - Molecular Biology 2020Quote: ... the cells were treated with 1 µg/ml doxycycline for 24 h and single GFP+ cells were sorted into 96-well plates on a BD FACSAria UV instrument (BD Biosciences) and grown until colonies formed.
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) plus 1 µg/ml ionomycin for 4 h at 37°C in the presence of GolgiPlug/GolgiStop (BD Pharmigen). Cells were stained for surface molecules as described above ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed once with FACS buffer and fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). In some experiments ...
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bioRxiv - Microbiology 2021Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Immunology 2021Quote: ... lamina propria cells were stimulated for 5 h with PMA (50 ng/ml) and ionomycin (500 ng/ml) in the presence of BD Golgi plug (BD Biosciences) followed by fixation and permeabilized using the BD Cytofix/Cytoperm kit ...
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bioRxiv - Cell Biology 2019Quote: ... GFP+ cells were sorted in 96-well plates 48 h post transfection using a FACS Aria III cell sorter (BD BioSciences). Single cells were grown until formation of viable individual clone in DMEM medium supplemented with 50% fetal bovine serum and 1% penicillin/streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Footpads were digested with 4 mg ml−1 collagenase D for 2 h at 37°C before dissociation through 70 μm cell strainers (BD Biosciences). Homogenates were resuspended in HOMEM supplemented with 20% FCS and serial dilutions (2-fold ...
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bioRxiv - Immunology 2020Quote: Supernatants were collected after 4 or 24 h and the levels of TNF and IL-1β were quantified by ELISA using OptEIA human TNF ELISA Set (BD Biosciences) and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... cells were restimulated ex vivo with PMA (50 ng/ml) and ionomycin (750 ng/ml) for 4 h in the presence of GolgiPlug (BD Bioscience), and then fixed and permeabilised following intracellular staining with anti-mouse IL-17A (clone ReBio17B7 ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h and analyzed for fluorescence expression using a flow cytometer (BD FAC AriaIII sorter; BD biosciences, New Jersey, USA). A viral titration curve was constructed using viral dilutions on the X-axis and percentage EGFP+ve cells on the Y-axis ...
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bioRxiv - Neuroscience 2023Quote: ... single viable cells were gated and CD45-O1+ and CD45lowSiglec- H+ cells were collected using a FACSAria III and corresponding software (FACSDiva, v.6; BD Biosciences). Calculation of the number of CD45lowSiglecH+ microglia per brain was performed by extrapolating their frequency to the counted total number of isolated cells ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated with 1 µg/ml SIINFEKL peptides in complete RPMI medium for 1 h at 37°C followed by brefeldin A (Golgiplug; BD Pharmingen) addition and further 5 h incubation ...
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bioRxiv - Cell Biology 2023Quote: ... 10-μM bromodeoxyuridine (BrdU) solution was added 4 h before the end of the experiment [fluorescein isothiocyanate (FITC) BrdU Flow Kit (BD #559619)] according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and the cells were cultured for 24 h prior to FACS sorting of transduced (GFP+/YFP+) cells on a BD FACSAriaTM III (BD Bioscience). The efficiency of shRNA-mediated gene expression knockdown was assessed with qPCR and cells were used for transplantation and assessment of their competitiveness in vivo.
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bioRxiv - Molecular Biology 2023Quote: ... while the OR10a/49b/71a cell lines were sorted 48 h after doxycycline addition for GFP and mCherry fluorescence with a FACSAria III cell sorter (BD Biosciences) into 96-well cell culture microplates ...
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bioRxiv - Immunology 2023Quote: 50 × 103 effector cells were cultured alone or together with 5 × 103 tumour cells for 4 h at a 10:1 ratio in the presence of anti-CD107a-PE mAb (clone: H4A3; BD Biosciences) and monensin (3 μM ...
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bioRxiv - Immunology 2023Quote: ... Donor DCs arriving in the draining lymph node (popliteal and inguinal respectively) were enumerated 48 h later by flow cytometry using a BD FACSLyric flow cytometer (BD BioSciences).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were stained for 30 min at 4 °C and washed with cell staining buffer and acquired within 1 h on a custom SORP five laser FACSAria Fusion (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... 1 μg/ml each of ESAT-6 and CFP-10 peptide pools (provided by Aeras, Rockville, MD) or R10 media for 2 h before adding 10 μg/ml BD GolgiPlug (BD Biosciences) for a further 12 hours ...
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bioRxiv - Immunology 2023Quote: ... prior to stimulation for 5 h with anti-CD3 and anti-CD28 without IL-2 in the presence of brefeldin A and monensin (BD Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... E8.5 embryos were fixed with 4% paraformaldehyde (PFA) in PBS at 4°C for 3 h and incubated with FITC-conjugated anti-EEA1 (1:100, BD Bioscience) or Cy3-labeled anti-mouse IgG (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... IL-6 and IL-1β cytokines were measured in the cell culture supernatant after 2 h of co-exposure using CBA human Inflammatory cytokine kit (BD Biosciences), while chemokines CXCL8/IL-8 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The activated Ramos cells were incubated overnight (18 h) in 5% CO2 at 37°C followed by staining for flow cytometry (BD LSR2) and analysis (FlowJo and Graphpad Prism).
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bioRxiv - Cell Biology 2024Quote: Cells were seeded 24 h before treatment and were allowed to grow in the presence of 3 μg/mL BrdU (BD Pharmingen) for 48 h ...
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bioRxiv - Bioengineering 2024Quote: ... and the hemoglobin contents were quantified after 8 h by the colorimetric assay kit (absorbance: 550 nm; with BD Pharm Lyse). Each value was subtracted from the internal control without the anti-BSA antibody.
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bioRxiv - Neuroscience 2021Quote: ... followed by the primary antibodies incubation (for the mouse tissue: mouse anti-aSyn antibody (Syn1) (BD Transduction Laboratory ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained with the following antibodies: anti-mouse antibody to CD45 (30-F11; BD Biosciences), CD45.1 (A20 ...
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bioRxiv - Immunology 2020Quote: ... and stained with IFN-γ FITC (B27) antibodies to detect intracellular cytokines (all BD Biosciences antibodies). In all assays described above ...
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bioRxiv - Genomics 2019Quote: ... The antibodies used were: phycoerythrin (PE)- mouse anti-human anti–CD41a antibody (BD Pharmingen; Cat# 555467); fluorescein isothiocyanate (FITC)- mouse anti-human Annexin-V (biolegend ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-PODXL2 Antibody (211816) (R & D Systems) or mouse anti-CD34 Antibody (563) (BD Biosciences) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were pooled on ice with 2.2 μl per antibody per 1×106 cells (BD Genomics). Cells were quenched with 2 ml 1% BSA in PBS and spun at 350xg for 5 minutes and further washed two more times with 2 ml of 1% BSA in PBS ...
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bioRxiv - Immunology 2021Quote: ... All antibodies were purchased from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... anti-PI4KIIIβ antibody (611816, BD Bioscience); anti-GAPDH (G9545 ...
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bioRxiv - Molecular Biology 2021Quote: ... and Ki-67 antibody (BD Biosciences). Cells were incubated with primary antibodies for 16 hours at 4°C ...
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bioRxiv - Immunology 2022Quote: ... All antibodies were purchased from BD Bioscience ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were from BD Biosciences ...