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Citations for Becton, Dickinson and Company :
451 - 500 of 4425 citations for 7H Pyrrolo 2 3 d pyrimidine 7 3 5 bis O 2 4 dichlorophenyl methyl 2 C methyl b D ribofuranosyl 4 chloro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated at 4°C overnight with primary antibodies (Ki67 BD Biosciences #556003 at 1:200 ...
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bioRxiv - Immunology 2024Quote: ... 25 min at 4°C using the BDCytofix/Cytoperm kit (BD Biosciences) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... or mouse monoclonal antibodies (eIF2α clone D-3, Santa Cruz #sc-133132, β-Actin clone E4D9Z, Cell Signaling Technology #58169; Phosphoserine/threonine clone 22A, BD Transduction Laboratories # 612548) were added to the blocking solution at dilutions ranging from 1/500 to 1,5000 according to the manufacturer’s datasheets ...
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bioRxiv - Immunology 2023Quote: ... 5 μg/mL of anti-IL-4 (BD Pharmingen, REF: 554385) and 10 ng/mL of IL-2 (R&D systems ...
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bioRxiv - Cancer Biology 2022Quote: ... were plated around the rim of the well of a 96-well plate in a 4:3 mixture of Matrigel and MammoCult medium (BD Bioscience CB-40324). The cell mixture was then incubated at 37°C with 5% CO2 for 15 min to solidify the gel ...
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bioRxiv - Systems Biology 2022Quote: Single cell suspensions were plated around the rim of the well in the 3:4 mixture of Mammocult and Matrigel (BD Bioscience CB-40324). We seed 5’000 cells/wells for mini-rings in white 96-well plates (Corning #3603 ...
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bioRxiv - Immunology 2021Quote: ... both ends of femurs and tibiae were cut with surgical scissors and bone marrow was extracted by flushing 3-5 ml sterile and ice-cold buffer through the bones using a 26G needle (BD Microlance 3 300300). The isolated bone marrow was then filtered through a 40 µM nylon cell strainer (FALCON 352340 ...
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bioRxiv - Microbiology 2019Quote: ... 3 (BD ref211693), 10 g glycerol ...
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bioRxiv - Microbiology 2020Quote: ... 3 (BD Biosciences), 0.5 g/l soluble starch (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... 3 (BD Biosciences), 5 g of Na2HPO4 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were rinsed with 2 × 2 ml Pharmingen stain buffer and fixed with 4.21% (w/w) formaldehyde (BD Biosciences, #554655). Cells were analyzed on LSRFortessa X-20 (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... albicans MTL a/α clinical isolate SC5314 72 was used for all experiments and was routinely grown in YPD (1% yeast extract, 2% peptone, 2% glucose; all BD Biosciences) at 30 °C.
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bioRxiv - Immunology 2021Quote: ... To this end 1×104 targets per well and 2×104 effector cells per well were each preincubated for 2 h in 10 μg/ml anti-human CD178 antibodies (NOK-1 and NOK-2, BD Biosciences) or 5 μg/ml anti-human TRAIL antibody (RIK-2 ...
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bioRxiv - Immunology 2023Quote: ... All flow cytometry analyses were performed in FACS buffer containing PBS with 2 mM EDTA and 2% FBS on a FACS CANTO (BD Pharmingen) and analysed with FlowJo software (Treestar).
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bioRxiv - Cancer Biology 2023Quote: ... CD49fhighEpCAMlowLin- cells from 2-month-old C57BL/6 WT mice were sorted using FACS and resuspended in culture media (DMEM/F12+2% FBS+1% PSA+2% B27) supplemented with EGF (10 ng/ mL, BD Bioscience), Rspo1(250 ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... CD49fhighEpCAMlowLin- cells were collected from FACS and cultured in 200 μL culture media (DMEM/F12+2% FBS+1% PSA+2% B27) supplemented with 10 ng/ mL EGF (BD Bioscience), 250 ng/ mL Rspo1(R&D) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with 2% ECL prime blocking reagent in PBS-T (Amersham; #RPN418) and incubated against AP-2 (mouse-anti-AP50, BD, #A611351) and GAPDH (rabbit-anti-GAPDH ...
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bioRxiv - Microbiology 2023Quote: ... and values for IL-2 were determined by using a standard curve generated by using known concentrations of purified recombinant murine IL-2 (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...
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bioRxiv - Immunology 2023Quote: ... Samples were stored in PBS containing 2% (V/V) FBS and 2 mM EDTA until acquisition on an LSR Fortessa flow cytometer (BD Biosciences). Analyses were performed with FlowJo v10.7.1 (BD Biosciences).
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bioRxiv - Biophysics 2023Quote: ... Cells were then washed twice in FACS buffer (PBS, 2% FBS, 2 mM EDTA) and permeabilized in BD Phosflow Perm Buffer III (BD Biosciences), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with ice-cold FACS buffer (DPBS supplemented with 2% FBS and 2 mM EDTA) and subjected to fixation and staining with Cell Fixation and Permeabilization Kits (BD Biosciences). Incubation with antibodies was performed for 1h at RT ...
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bioRxiv - Biophysics 2021Quote: ... filled with motility buffer containing various concentrations of α-methyl-aspartate (MeAsp) through polyethylene tubing (Polythene Tubing, 0.58 mm id, 0.96 mm od; BD Intermedic). The tubing was connected to the PMDS device through stainless steel pins that were directly plugged into the inlets or outlet of the device (New England Tubing) ...
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bioRxiv - Developmental Biology 2021Quote: ... the E13.5 dams were injected intraperitoneally with 200 μl of 5-bromo-2’-deoxyuridine (BrDU) (552598, BD Pharmingen) early in the morning and the embryonic kidneys were collected 2.5 hours later ...
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bioRxiv - Genomics 2021Quote: ... supplemented with 10% modified ADC (2% glucose, 5% BSA, 0·85% NaCl) or in Middlebrook 7H9broth (BD Biosciences) supplemented with 10% modified ADC along with 0.05% Tween 80 without shaking.
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bioRxiv - Immunology 2021Quote: ... then washed and resuspended in PBS-2% FBS for flow acquisition on a 5-laser LSRII (BD BioSciences). For experiments with intranuclear transcription factor staining ...
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bioRxiv - Developmental Biology 2023Quote: 5-ethynyl-2’-deoxyuridine (EdU) staining was performed using the 647 EdU Click Proliferation Kit (BD Biosciences, 565456). First ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed using 4% PFA for 15 minutes at 4°C and permeabilized for intracellular cytokine staining using Perm/Wash Buffer (BD Perm/WashTM). For intracellular staining ...
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bioRxiv - Systems Biology 2019Quote: ... Membranes were incubated with primary antibody solutions (using 5% milk or BSA in TBST) overnight at 4°C at the respective dilutions: E-Cadherin (BD, #610181,1:5000), Vimentin (Abcam ...
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Immunology 2019Quote: Cells were harvested by centrifugation at 400 g for 5 minutes at 4°C followed by staining with fixable viability stain (BD Horizon FVS700) for 10 minutes on ice in PBS ...
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bioRxiv - Immunology 2022Quote: ... 7-AAD (5 µl, BD, #559925) and 1.75 µl of 85 mM CaCl2 were added ...
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bioRxiv - Neuroscience 2023Quote: ... and poly-D Lysine (BD biosciences) before being incubated at 37°C ...
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bioRxiv - Immunology 2023Quote: ... 3 to 5 colonies were transferred into trypticase soy bouillon (TSB; BD, Germany) and cultivated under shaking for 3 hours at 37 °C to receive growth state bacteria ...
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bioRxiv - Immunology 2023Quote: ... 3 to 5 colonies were transferred into trypticase soy bouillon (TSB; BD, Germany) and cultivated under shaking for 3 hours at 37 °C to receive growth state bacteria ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at room temperature followed by overnight incubation at 37°C in antibodies to the following: (1) C-terminal binding protein 2 (mouse anti-CtBP2; BD Biosciences, used at 1:200). (2 ...
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bioRxiv - Bioengineering 2023Quote: Splenocytes from 125Tg B6 and WT B6 control mice were stained with CellTrace Violet and cultured for 3 days35 in complete RPMI alone or stimulated with 5 ug/mL anti-mouse anti-CD40 (BD Pharmingen clone HM40-3) in the presence or absence of conjugates described above at 150 nM concentrations ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 infected cells were fixed in 2% formaldehyde for 30 mins and permeabilized with BD Perm/Wash buffer (BD Biosciences, 554723). The primary antibody used was sheep anti-SARS-CoV-2 nucleocapsid antibody (DA114 ...
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bioRxiv - Immunology 2021Quote: ... 50 U/ml Penicillin and 50 ug/ml Streptomycin) supplemented with 100 U/ml hIL-2 and 2 ug/ml anti-CD28 (BD Pharmingen, 553294). Two days after the primary stimulation ...
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bioRxiv - Immunology 2023Quote: ... were diluted 1:50 in PBS-10% FBS and IL-2 concentration was determined by ELISA assays using the OptEIA Human IL-2 ELISA commercial kit (BD Biosciences, USA) and revealed with TMB substrate (Thermofisher ...
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bioRxiv - Immunology 2022Quote: ... all cells were stained for L/D staining and acquisition was performed on the Fortessa LSR flow cytometer (BD B ioscience) and analyzed with Kaluza (Beckman Coulter) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mL of dispase (BD Biosciences, 50 U/ml) was instilled into the lungs through the trachea until the lungs inflated ...
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bioRxiv - Immunology 2022Quote: ... TCR Vβ14-Biotin (BD Bioscience: 553257, clone: 14-2), Streptavidin-BV421 (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Cultures were supplemented with 10IU IL-2 (BD Biosciences) 2-3 times weekly ...
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bioRxiv - Immunology 2019Quote: ... and CD49d (BV786, R1-2 1/300 BD Biosciences). Intracellular IFN-γ (APC ...
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bioRxiv - Bioengineering 2020Quote: ... 2% Bacto™-agar (BD, Franklin Lakes, NJ, USA) was added to make agar plates.
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bioRxiv - Microbiology 2021Quote: ... in Noble Agar (BD DIFCO™, 2% w/v), cooling to 50°C with agitation ...
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bioRxiv - Biochemistry 2021Quote: ... Cells resuspended in 2% BSA were analyzed by BD LSR Fortessa or LSRII SORP (Becton Dickinson) ...
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bioRxiv - Immunology 2022Quote: ... and ERK1/2 (pT202/pY204) were purchased from BD Biosciences ...
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bioRxiv - Genomics 2020Quote: ... Data were collected on LSRII or Fortessa 2 (BD) and were analyzed with FlowJo software (TreeStar).