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Citations for Becton, Dickinson and Company :
451 - 500 of 989 citations for 7 Oxa 12 thia 3 aza spiro 5.6 dodecane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-cleaved caspase-3 (1:200; 559565, BD Pharminogen) and rabbit anti-cleaved caspase-3 (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse monoclonal anti-IP3R-3 antibody was purchased from BD Biosciences (San Jose ...
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bioRxiv - Immunology 2023Quote: ... and stained with anti-active caspase 3 antibody (BD Biosciences). For KDM2A staining ...
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bioRxiv - Immunology 2023Quote: ... Prior to cell sorting on the Aria 3 (BD Biosciences) and during cell surface antibody staining ...
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bioRxiv - Immunology 2023Quote: ... Prior to cell sorting on the Aria 3 (BD Biosciences) and during cell surface antibody staining ...
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bioRxiv - Neuroscience 2024Quote: ... RT for 3 min before staining with Annexin V (BD Pharmingen PE Annexin V apoptosis detection kit ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were resuspended in ice cold PBS and supplied with 10 μl of 7-AAD to assess cell viability (BD Pharmigen) and sorted with a BD Aria III FACS as previously described 30 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Adherent and suspension cells of each sample were pooled and after centrifugation for 5min at 800 x g cells were carefully resuspended in cell culture medium with 10 µl/ml 7-AAD (BD Biosciences). After 10 minutes incubation in the dark cells were immediately analyzed by flow cytometry using a FACS Aria III (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... washed twice and resuspended in 200 μL of blocking buffer in the presence of 7-AAD (BD Biosciences Cat#: 51-68981E) or DAPI (Invitrogen Cat# ...
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bioRxiv - Cell Biology 2022Quote: ... Cell survival was measured by FACS analysis after staining cells with a membrane-impermeable DNA binding dye (DAPI, PI, or 7-AAD) and/or annexin V (BD Pharmingen).
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bioRxiv - Developmental Biology 2022Quote: ... Cells from mouse tail skin (aging RNA-seq) and dorsal/ventral skin (fibulin 7 RNA-seq) were isolated using FACS Aria flow cytometer (BD Biosciences). Data were analyzed using FlowJo software (BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... Myoblasts were stained with 7-AAD (cell viability) and with the positive selection marker anti-CD56 AF647-conjugated (clone R19-760, BD biosciences). Of note ...
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bioRxiv - Immunology 2020Quote: ... The cells were then stained with 7-AAD (live/dead marker) prior to flow cytometric sorting on an FACS Aria II sorter (BD Biosciences). The sorted germinal centre B cells (7-AAD−/B220+/GL-7+/Fas+ ...
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bioRxiv - Immunology 2020Quote: ... A fixable Viability Dye (APC-eFluor780 1:200, 1:400) (eBioscience, Germany) or ViaProbe (7-AAD, 1:33) (BD Biosciences, Germany)) was used for live/dead discrimination ...
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bioRxiv - Immunology 2021Quote: ... and then labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, FITC anti-mouse CD4, APC-Cy®7 mouse anti-CD8, all from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: Mononuclear cells were directly labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, clone, FITC anti-mouse CD4, APC-Cy®7 anti-mouse CD8, for T lymphocyte phenotype, all from BD Biosciences ...
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bioRxiv - Genomics 2020Quote: ... ODInCas9 system was left to turn off for 7 days post transfection before assessment using FACS instrument BD LSRFortessa (BD Bioscience) and confocal microscopy instrument Cell Voyager 7000S (CV7000S ...
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bioRxiv - Microbiology 2021Quote: ... The infectivity was measured by detecting GFP fluorescence using a Zeiss fluorescence microscope (AXIO observer 7) and measured as the percentage of GFP positive cells analyzed with a Fortessa X20 flow cytometer (BD Biosciences) at 24 hours post infection ...
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bioRxiv - Immunology 2020Quote: ... then washed with 2% FCS - PBS and stained with Mouse anti-IRF-7-PE (pS477/pS479, clone K47-671; BD Biosciences) or Mouse anti-NF-κB p65-Alexa Fluor 647 (pS529 ...
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bioRxiv - Cell Biology 2021Quote: ... macrophages were incubated with 5 μM 2’, 7’ dichlorofluorescein diacetate (DCFDA, Invitrogen at 37°C for 30 min, followed by acquisition using FACS Canto I (BD Sciences) within 30 minutes of staining ...
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bioRxiv - Cell Biology 2019Quote: ... Cells with normal mCherry fluorescence (mid 70-80% fluorescence) and top or bottom ~7% eGFP fluorescence (~4 million cells/population) were sorted using a BD FACSAria III (BD Biosciences) and collected into 15 ml polypropylene tubes (Cellstar ...
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bioRxiv - Immunology 2020Quote: ... Spleen B cells were analyzed for BrdU incorporation (by anti-BrdU mAb staining) and DNA contents (by 7–AAD staining after cells were permeabilized) using the APC BrdU Flow kit® (BD) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Microbiology 2021Quote: ... Infectivity was assessed by detecting GFP fluorescence using a Zeiss fluorescence microscope (AXIO observer 7) and/or determined by the percentage of GFP(+) cells analyzed using a Fortessa X20 flow cytometer (BD Biosciences) at 16 ...
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bioRxiv - Immunology 2022Quote: ... Human CD34+ stem cell purity was monitored by anti-human CD34 FITC (clone:581) and 7-AAD viability dye using LSR Fortessa X-20 flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 7 days and immunostained for flow cytometry (Antibodies: CD34, clone 581; CD117, clone: 104D2; CD11b, clone ICRF44 (BD Pharmingen) and 1:50 FcR Blocking Reagent (Miltenyi Biotec)) ...
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bioRxiv - Biochemistry 2023Quote: Cells were grown in synthetic complete medium at 25°C to mid-log phase after 7-8 doublings and harvested for direct fluorescent measurement of RFP and YFP channels by an LSR Fortessa (BD biosciences) flow cytometer ...
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bioRxiv - Cell Biology 2022Quote: ... 5 × 106 MCF-7 cells stably expressed shCtrl and shMYSM1 lentivirus were suspended in 100 μl of culture medium/Matrigel (1:1) (BD Biosciences) and implanted subcutaneously into the bilateral armpits of 4-week-old female BALB/c nude mice (Vital River Laboratory) ...
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bioRxiv - Developmental Biology 2023Quote: ... or (ii) PE-Annexin V conjugated antibody (1:33 dilution in TBST) and 7-AAD as per kit instructions (BD Pharmingen). Samples were analysed by flow cytometry within 30 min.
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bioRxiv - Immunology 2023Quote: ... from naïve/PBS-treated or i.n fusion protein immunized mice were isolated from mLN from a pool of 7-8 C57Bl/6 mice and FACS-sorted on a Aria II cell sorter (BD Bioscience). Labeling of cells prior to sorting by flowcytometry (Fusion ...
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bioRxiv - Cancer Biology 2023Quote: ... and total DNA (7-AAD 1:50 solution dilution) and analyzed by flow cytometer in a BD LSRFortessa cell analyzer (BD Biosciences) using 488 nm and 640 nm lasers ...
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bioRxiv - Biochemistry 2023Quote: ... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.
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bioRxiv - Microbiology 2023Quote: Bacteria used in this work are listed in Supplementary Table 7 and were grown at 37 °C in brain and heart infusion (BHI) broth (with agitation) and agar media (BD Difco). Cell lines of human origin used in this work included JEG-3 trophoblasts (ATCC HTB-36) ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclear extract was prepared by dounce homogenization in cold swelling buffer (using a size 7 dounce) and filtered using the cell strainer (100 μm, BD Biosciences). Lysate was centrifuged at 400 g for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... The resulting tissue homogenates were each serially diluted from 10-1 to 10-7 and plated in triplicates onto Marine Agar 2216 (MA, BD Difco). Following one week of incubation at 23 °C ...
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bioRxiv - Immunology 2024Quote: ... Cell apoptosis was measured by co-staining cells with Annexin V and 7-AAD using the PE Annexin V Apoptosis Detection Kit I (BD Biosciences), according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... OPs were obtained by immuno-panning from single-cell suspensions of dissociated P6-7 mouse cortices using anti-PDGFRA immunoglobulin (558774, BD Pharmingen) as previously described (Emery and Dugas ...
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bioRxiv - Biophysics 2020Quote: ... we punched 12-mm diameter holes in the center of 50×9-mm Falcon petri dishes (BD Falcon). We then attached treated glass coverslips to the bottoms of the petri dishes with their treated sides face up using vacuum grease (Beckman Vacuum Grease Silicone) ...
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bioRxiv - Cell Biology 2021Quote: ... Then CD45+ cells were then incubated with Ly6C-PE (eBioscience 12-5932) and CD64-APC (BD Pharmingen 558539) antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 1×104 cells/well of early and late passage were seeded in 12-well culture plates (BD Falcon). Cell number and viability were examined each day in triplicate wells using trypan blue exclusion and cell proliferative assay (# Cat ...
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bioRxiv - Microbiology 2023Quote: ... The animals (12-14 weeks post-engraftment) were treated with 1 ml of 4% thioglycolate (Brewer’s medium; BD) via intraperitoneal (IP ...
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bioRxiv - Cancer Biology 2021Quote: ... and anti-cleaved caspase 3 (clone C92-605, BD Biosciences, #559565). After staining ...
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bioRxiv - Cell Biology 2020Quote: ... and plated 3 days before analysis on an LSR II (BD). Controls cultured in the same conditions included cells transduced with guides against 3 non-growth limiting genes ...
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bioRxiv - Developmental Biology 2021Quote: The hiPSC line PLA-1-3 was maintained on Matrigel (BD)-coated plates in mTeSR medium (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... and 3 μg/mL anti-mouse CD45 APC (BD Bioscience, 559864) in PBS supplemented with 1 % BSA and 0.1 % Tween 20 (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... and 3 ml Wash3 buffer (BD buffer with 75 mM imidazole). Proteins bound to the column were eluted with 3 ml elution buffer (BD buffer with 100 mM imidazole ...
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bioRxiv - Cancer Biology 2022Quote: ... then fixed in 1:3 fixation/permeabilization concentrate: diluent mixture (BD) for 15 minutes ...
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bioRxiv - Immunology 2020Quote: ... streptavidin-HRP and 3-amino-9-ethylcarbazole (AEC) substrate (BD Bioscience) were added and spots counted using a CTL ImmunoSpot S6 Universal Analyzer (CTL) ...
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bioRxiv - Immunology 2022Quote: ... anti-Ly6C-FITC (3 µg/ml, clone AL-21; BD Bioscience), anti-Ly6G-PE-CF594 (0.4 µg/ml ...