Labshake search
Citations for Becton, Dickinson and Company :
201 - 250 of 303 citations for 7 Chlorotryptamine HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... 1:50000 in 1% BSA PBS pH 7 and analysed by cytofluorimetry in a FACSCalibur (BD Biosciences).
-
bioRxiv - Cell Biology 2022Quote: ... centrifuged and resuspended in 200μl of PBS 0.1% BSA and stained for 7-AAD (BD –Pharmingen 559925) for 10’ at RT protected from light before being analyzed in BD LSRFortessa Cell Analyzer ...
-
bioRxiv - Cancer Biology 2021Quote: ... A total of 5× 106 MCF-7 cells were resuspended in 200 μL of Matrigel (BD Biosciences) and PBS (1:1 ...
-
bioRxiv - Developmental Biology 2020Quote: ... passed through a 30 μm cell strainer and incubated with 10 μl/ml 7-ADD (BD Biosciences) for 5 min in ice in the dark ...
-
bioRxiv - Cancer Biology 2019Quote: ... Apoptosis was quantified by FACS upon staining cells with PE Annexin-V and 7-ADD (BD Biosciences). Cells were sorted using the FACS Aria II (BD Biosciences) ...
-
bioRxiv - Immunology 2023Quote: ... IRF7 (Alexa Fluor® 647 conjugated Mouse anti-IRF-7 [pS477/pS479], clone: K47-671, BD Biosciences), IRF3 (PE conjugated Rabbit mAb Phospho-IRF-3 [Ser396] ...
-
bioRxiv - Cell Biology 2023Quote: ... 7 µm cryosections for capillary density were stained with anti-mouse CD31 antibody (MEC 13.3, BD Bioscience). For immunohistochemistry ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell viability was assessed using flow cytometric analysis using Annexin-V FITC and 7-AAD (BD Biosciences).
-
bioRxiv - Genetics 2021Quote: ... 0.5 mM EGTA and 30 mM Tris–HCl pH 7.4) using 25 ml total pushed through a 30 ml syringe with a 26G needle (BD Precision Glide, cat. number 305111) into the left ventricle of the beating heart ...
-
bioRxiv - Cancer Biology 2019Quote: ... They were washed twice with PBS + 1% BSA before incubating with 7-Amino-actinomycin D (7AAD) (BD Biosciences) for 10 min ...
-
bioRxiv - Microbiology 2019Quote: ... Bacterial strains were routinely grown in Difco Lennox broth pH 7 (LB; Becton Dickinson [BD], Franklin Lakes, NJ). For two-hybrid system interactions ...
-
bioRxiv - Cell Biology 2022Quote: ... The cell pellet was then resuspended in ice-cold PBS containing 7-amino-actinomycin D (BD Pharmingen, 559925) diluted 1:100 ...
-
bioRxiv - Neuroscience 2019Quote: ... Cells were resuspended in 1 ml of ice-cold PBS and 10 µl of 7-AAD (BD Pharmingen) were added for dead cells discrimination ...
-
bioRxiv - Cancer Biology 2021Quote: ... 25 mM HEPES pH 7) and analyzed on a BD Accuri C6 cytometer (BD Biosciences, San Jose, CA). As a control ...
-
bioRxiv - Immunology 2022Quote: ... 7 mL of EDTA-anticoagulated whole blood was treated with 45 mL BD Pharm lysis buffer (BD Biosciences) to completely lyse red blood cells ...
-
bioRxiv - Cancer Biology 2022Quote: ... Apoptosis was detected by staining with 7-AAD and Anexin V-PE (Apoptosis Detection Kit I - BD Pharmingen). Attune NxT flow cytometer (Life Technologies ...
-
bioRxiv - Biochemistry 2023Quote: Three Alistipes and two Odoribacter strains (Supplementary Table 7) were cultured in Reinforced Clostridial Medium (RCM, BD Biosciences) under anaerobic conditions at 37°C ...
-
bioRxiv - Cancer Biology 2024Quote: Apoptosis was determined by flow cytometry using the Annexin V-FITC/7-AAD Apoptosis Detection Kit (BD Biosciences) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were collected and stained with BrdU-APC and 7-AAD using BD Pharmingen BrdU Flow Kit (BD, 552598) following manual instructions ...
-
bioRxiv - Immunology 2019Quote: ... Cells were resuspended in 0.5ml of Stain Buffer (FBS) and incubated with the viability dye 7-AAD (BD Biosciences) at RT for 10 minutes prior to acquisition on a 3-laser ...
-
bioRxiv - Physiology 2020Quote: ... the pellet was resuspended in 7 mL and filtered (Cell Strainer 70 um Nylon; BD Falcon, Bedford, MA, USA). The islets in the filter were washed twice with HBSS and plated in a RPMI solution by inverting the filter (RPMI ...
-
bioRxiv - Cancer Biology 2022Quote: ... Apoptosis was quantified using allophycocyanin-conjugated Annexin V and viability was assessed with 7-Amino-actinomycin D (BD Bioscience). Gating was defined using untreated/unstained cells and treatments were evaluated at 20,000 counts using BD FACSVantage SE.
-
bioRxiv - Molecular Biology 2023Quote: ... A small aliquot of cells were stained in PBS 1x with the viability marker 7-AAD (BD Biosciences, 559925) and T-cell marker CD4 FITC (clone GK1.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells were stained with FITC-conjugated Annexin V and 7-AAD according to the manufacturer’s instructions (BD Biosciences) and analysed using a LSRFortessa X-20 cytometer and FlowJo software (RRID:SCR_008520 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Samples were then diluted 1:1 with ice cold 0.5% BSA/CMFNM and stained with 60 μl/ml 7-AAD (BD, 559925) for > 20 minutes on ice ...
-
bioRxiv - Immunology 2021Quote: ... fixed and stained with APC anti-BrdU antibody and 7-AAD using the APC BrdU Flow kit (BD Pharmingen, 552598) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... Trypsinized cells were processed for BrdU-FITC and 7AAD (7-Aminoactinomycin D) staining following the manufacturer’s protocol (BD Pharmigen, 559616). FACS was run on a Scanford analyzer (Stanford FACS Core Facility ...
-
bioRxiv - Cell Biology 2021Quote: ... FITC Annexin V antibodies and 7-AAD were loaded into 100 μL cells suspension aliquot to analyze pro-apoptosis following the manufacturer’s instructions (BD Biosciences). FITC isotypes were used as the isotope control ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were grown for ∼7 days and subcloned by single cell sorting on a BD FACSAria UV instrument (BD Biosciences). Thap1-/- clones were confirmed by Sanger sequencing as described above.
-
bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
-
bioRxiv - Immunology 2020Quote: Samples of stimulated CD45.2+ OT-I cells and CD45.1+ wild type splenocytes mixtures were stained with CD8a and CD45.2 antibodies and 7-AAD and OT-I cells were sorted (BD FACSAria) into 100% FBS and pelleted.
-
bioRxiv - Microbiology 2021Quote: ... the splenocytes were stained with the extracellular markers PE-Cy ™ 7 Hamster Anti-Mouse CD3e (Cat. # 552774, BD Pharmingen), APC-Cy7 Rat Anti-Mouse CD4 (Cat ...
-
bioRxiv - Genetics 2020Quote: ... we also collected 5-7 ml of peripheral blood in a tube containing the anticoagulant K3 EDTA (#6457, BD Biosciences) which was used for flow cytometry as described below ...
-
bioRxiv - Genomics 2021Quote: Each strain was cultivated for 3-7 days in 10 mL of Tryptic Soy broth (BD Diagnostic, catalog no. 211768) with 0.5% (w/v ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-F4/80-PE clone REA126 and human anti-CD14-FITC from Miltenyi Biotech and 7-AAD staining solution from BD Pharmingen ...
-
bioRxiv - Immunology 2020Quote: ... The cell viability dye 7-AAD (Via-Probe) and Live/dead fixable Aqua fluorescent reactive dye were purchased from BD Biosciences and ThermoFisher ...
-
bioRxiv - Cancer Biology 2022Quote: ... Images were analysed using Cellprofiler80 with default settings to quantify the cytoplasmic to nuclear intensity ratios.The following antibodies were used for IF: p53 (DO-7 FITC conjugate, BD biosciences), USP28 (Bethyl A300-898A) ...
-
bioRxiv - Cell Biology 2022Quote: ... cultured PAECs were incubated with 2’,7’–dichlorofluorescin diacetate (DCFDA; 1μM) for 10 minutes at 37°C and then assessed by flow cytometry (BD LSRFortessa).
-
bioRxiv - Immunology 2022Quote: ... HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml, BD Biosciences) and CD28 (1 μg/ml ...
-
bioRxiv - Developmental Biology 2022Quote: ... Samples were then diluted 1:1 with ice cold 0.5% BSA/CMFNM and stained with 60 µg/ml 7-AAD (BD, 559925) for more than 20 min on ice ...
-
bioRxiv - Immunology 2024Quote: ... at which point they were phenotyped (FOXP3-PeCy7, clone FJK-16s, Thermo Fisher Scientific; HELIOS-PEDazzle594, clone 22F6, Biolegend; Thy1.1-BV711, clone OX-7, BD Biosceinces ...
-
bioRxiv - Cancer Biology 2024Quote: NF1-proficient and NF1-deficient engineered clones were injected into the flanks of 6- to 7-week-old athymic nude mice (1.0×106 cells) in 50% Matrigel (BD Biosciences). Mice were monitored for tumor formation for 6 months post injection or until a tumor volume of 2000mm3 was reached.
-
bioRxiv - Bioengineering 2023Quote: ... Mice sera were collected retro-orbitally at 4 h and 7 days post administration and subjected to cytokine analysis (Cytometric Bead Array Mouse Inflammation Kit, BD) using flow cytometry following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were resuspended in ice cold PBS and supplied with 10 μl of 7-AAD to assess cell viability (BD Pharmigen) and sorted with a BD Aria III FACS as previously described 30 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
-
bioRxiv - Microbiology 2020Quote: ... Adherent and suspension cells of each sample were pooled and after centrifugation for 5min at 800 x g cells were carefully resuspended in cell culture medium with 10 µl/ml 7-AAD (BD Biosciences). After 10 minutes incubation in the dark cells were immediately analyzed by flow cytometry using a FACS Aria III (BD Biosciences ...
-
bioRxiv - Cell Biology 2021Quote: ... washed twice and resuspended in 200 μL of blocking buffer in the presence of 7-AAD (BD Biosciences Cat#: 51-68981E) or DAPI (Invitrogen Cat# ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell survival was measured by FACS analysis after staining cells with a membrane-impermeable DNA binding dye (DAPI, PI, or 7-AAD) and/or annexin V (BD Pharmingen).
-
bioRxiv - Developmental Biology 2022Quote: ... Cells from mouse tail skin (aging RNA-seq) and dorsal/ventral skin (fibulin 7 RNA-seq) were isolated using FACS Aria flow cytometer (BD Biosciences). Data were analyzed using FlowJo software (BD Biosciences).
-
bioRxiv - Cell Biology 2022Quote: ... Myoblasts were stained with 7-AAD (cell viability) and with the positive selection marker anti-CD56 AF647-conjugated (clone R19-760, BD biosciences). Of note ...