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Citations for Becton, Dickinson and Company :
651 - 700 of 2258 citations for 7 Chlorothieno 3 2 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and stained with anti-active caspase 3 antibody (BD Biosciences). For KDM2A staining ...
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bioRxiv - Immunology 2023Quote: ... Prior to cell sorting on the Aria 3 (BD Biosciences) and during cell surface antibody staining ...
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bioRxiv - Immunology 2023Quote: ... Prior to cell sorting on the Aria 3 (BD Biosciences) and during cell surface antibody staining ...
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bioRxiv - Neuroscience 2024Quote: ... RT for 3 min before staining with Annexin V (BD Pharmingen PE Annexin V apoptosis detection kit ...
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bioRxiv - Immunology 2019Quote: ... Half of the enriched B cells were stained with anti-rat IgM (Clone G53-238, BD Biosciences, San Jose, CA) conjugated to PE Cy7 ...
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bioRxiv - Immunology 2019Quote: ... cells were washed and permeabilized for 16 hours according to manufacturer’s instructions for staining with anti-FOXP3 (236A/E7, PE, eBioscience) and anti-Granzyme-B (GB11, BV421, BD Biosciences). Cells were then fixed for subsequent analysis on a Fortessa flow cytometer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... Naive and germinal center B cell purity (>90%) was determined by flow cytometry analysis of surface IgD (BD Biosciences 555778), CD77 (BioRad MCA579 ...
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bioRxiv - Immunology 2022Quote: ... B cells were also stained with following anti-human antibodies (all at 1:200 dilution): anti-IgD-BV650 (BD, 740594), anti-CD27-BV786 (BD biosciences ...
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bioRxiv - Microbiology 2020Quote: ... B cell Ca2+ influx was monitored by measuring the 379/450 nm emission ratio on a LSR Fortessa (BD Biosciences). Following 30 s of baseline measurement ...
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bioRxiv - Microbiology 2021Quote: ... CD21 BV786 (Clone B-LY4, #740969), HLA DR BB700 (Clone G46-6, #566480), CD27 APC R700 (Clone M-T271, #565116) all from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... B cells were also stained with following anti-human antibodies (all at 1:200 dilution): anti-IgD-BV650 (BD, 740594), anti-CD27-BV786 (BD biosciences ...
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bioRxiv - Immunology 2019Quote: ... and 9g4-naïve B cells (CD27− IgD+ 9g4−) were obtained by FACS sorting on a BD FACSAria II (BD Biosciences). Purified samples were pelleted and stored at −80°C.
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bioRxiv - Genomics 2019Quote: ... Dendritic cells and basophils were sorted as CD3−CD19−CD11c+HLA-DR+ (DCs) and CD3−CD19−CD123+ (basophils) using the following antibodies: CD11C-PECy7 (Clone B-ly6, BD), HLA-DR-APCCy7 (Clone G46-6 ...
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bioRxiv - Immunology 2019Quote: ... Primary human B cells: Human peripheral blood was collected by routine phlebotomy in heparinized vacutainers (BD Biosciences, San Diego, CA) and diluted 1:2 with PBS containing 2% FBS ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We quantified the bias as the proportion of droplets containing pure (A, B, C or D) and compared to mixed populations (AB, AC, AD, BC, BD, CD ...
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bioRxiv - Immunology 2020Quote: ... CD19+CD27+IgG+ memory or CD19+CD27-IgG- naive B cells were isolated using an Aria II sorter (BD Biosciences). The sorted B cells were then added to the irradiated CD40L+ 3T3 cells at a density of 500-25000 cells/well ...
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bioRxiv - Immunology 2022Quote: ... B cells were also stained with following anti-human antibodies (all at 1:200 dilution): anti-IgD-BV650 (BD, 740594), anti-CD27-BV786 (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... DOT cells were incubated after membrane labelling with anti-perforin-BV241 (dG9) and anti-granzyme B-BV510 (GB11) from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Live Naïve B cells were obtained by sorting the stained cells using a FACS (fluorescence-activated cell sorting) Aria (BD Biosciences) as 7-AAD-CD19+CD38-IgD+CD27- ...
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bioRxiv - Microbiology 2022Quote: ... 1 μg/ml of anti-CD28 (clone 37.51) and 1 μg/ml of anti-CD49d (clone 9C10-MFR4.B) mAbs (BD Biosciences). During the last 3h of incubation ...
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bioRxiv - Immunology 2023Quote: ... transitional (CD19+CD3- B220+CD93+) and mature B cells (CD19+CD3-B220+CD93-) on BD FACSAria Fusion and FACSAria II (BD), before cultured in complete RPMI-1640 media without/with 5 μg/mL goat anti-mouse IgM (Jackson ImmunoResearch ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2 ...
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bioRxiv - Biophysics 2023Quote: The FRET A/D values were mapped onto the thermodynamic model using one fluorescence coefficient (C1) to describe monomeric and low-FRET forms of BRAF in solution (B, BD), and one fluorescence coefficient (C2 ...
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bioRxiv - Bioengineering 2024Quote: ... the B cells were subjected to single-cell sorting using a BD FACSAria III (BD Biosciences, Franklin Lakes, NJ, USA) and collected into 96-well plates pre-loaded with lysis buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were resuspended in ice cold PBS and supplied with 10 μl of 7-AAD to assess cell viability (BD Pharmigen) and sorted with a BD Aria III FACS as previously described 30 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Adherent and suspension cells of each sample were pooled and after centrifugation for 5min at 800 x g cells were carefully resuspended in cell culture medium with 10 µl/ml 7-AAD (BD Biosciences). After 10 minutes incubation in the dark cells were immediately analyzed by flow cytometry using a FACS Aria III (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... washed twice and resuspended in 200 μL of blocking buffer in the presence of 7-AAD (BD Biosciences Cat#: 51-68981E) or DAPI (Invitrogen Cat# ...
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bioRxiv - Cell Biology 2022Quote: ... Cell survival was measured by FACS analysis after staining cells with a membrane-impermeable DNA binding dye (DAPI, PI, or 7-AAD) and/or annexin V (BD Pharmingen).
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bioRxiv - Developmental Biology 2022Quote: ... Cells from mouse tail skin (aging RNA-seq) and dorsal/ventral skin (fibulin 7 RNA-seq) were isolated using FACS Aria flow cytometer (BD Biosciences). Data were analyzed using FlowJo software (BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... Myoblasts were stained with 7-AAD (cell viability) and with the positive selection marker anti-CD56 AF647-conjugated (clone R19-760, BD biosciences). Of note ...
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bioRxiv - Immunology 2020Quote: ... The cells were then stained with 7-AAD (live/dead marker) prior to flow cytometric sorting on an FACS Aria II sorter (BD Biosciences). The sorted germinal centre B cells (7-AAD−/B220+/GL-7+/Fas+ ...
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bioRxiv - Immunology 2020Quote: ... A fixable Viability Dye (APC-eFluor780 1:200, 1:400) (eBioscience, Germany) or ViaProbe (7-AAD, 1:33) (BD Biosciences, Germany)) was used for live/dead discrimination ...
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bioRxiv - Immunology 2021Quote: ... and then labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, FITC anti-mouse CD4, APC-Cy®7 mouse anti-CD8, all from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: Mononuclear cells were directly labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, clone, FITC anti-mouse CD4, APC-Cy®7 anti-mouse CD8, for T lymphocyte phenotype, all from BD Biosciences ...
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bioRxiv - Genomics 2020Quote: ... ODInCas9 system was left to turn off for 7 days post transfection before assessment using FACS instrument BD LSRFortessa (BD Bioscience) and confocal microscopy instrument Cell Voyager 7000S (CV7000S ...
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bioRxiv - Immunology 2019Quote: ... NK92 cells or primary patient fibroblasts were incubated with BrdU for 12 hours prior to fixation and staining with 7-AAD (BD Biosciences). Cells were analyzed via FACS on a BD Fortessa with 18-color configuration and resulting data were evaluated using Kaluza (Beckman-Coulter ...
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bioRxiv - Microbiology 2021Quote: ... The infectivity was measured by detecting GFP fluorescence using a Zeiss fluorescence microscope (AXIO observer 7) and measured as the percentage of GFP positive cells analyzed with a Fortessa X20 flow cytometer (BD Biosciences) at 24 hours post infection ...
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bioRxiv - Cell Biology 2019Quote: ... Cells with normal mCherry fluorescence (mid 70-80% fluorescence) and top or bottom ~7% eGFP fluorescence (~4 million cells/population) were sorted using a BD FACSAria III (BD Biosciences) and collected into 15 ml polypropylene tubes (Cellstar ...
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bioRxiv - Immunology 2020Quote: ... Spleen B cells were analyzed for BrdU incorporation (by anti-BrdU mAb staining) and DNA contents (by 7–AAD staining after cells were permeabilized) using the APC BrdU Flow kit® (BD) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Microbiology 2021Quote: ... Infectivity was assessed by detecting GFP fluorescence using a Zeiss fluorescence microscope (AXIO observer 7) and/or determined by the percentage of GFP(+) cells analyzed using a Fortessa X20 flow cytometer (BD Biosciences) at 16 ...
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bioRxiv - Immunology 2022Quote: ... Human CD34+ stem cell purity was monitored by anti-human CD34 FITC (clone:581) and 7-AAD viability dye using LSR Fortessa X-20 flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 7 days and immunostained for flow cytometry (Antibodies: CD34, clone 581; CD117, clone: 104D2; CD11b, clone ICRF44 (BD Pharmingen) and 1:50 FcR Blocking Reagent (Miltenyi Biotec)) ...
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bioRxiv - Biochemistry 2023Quote: Cells were grown in synthetic complete medium at 25°C to mid-log phase after 7-8 doublings and harvested for direct fluorescent measurement of RFP and YFP channels by an LSR Fortessa (BD biosciences) flow cytometer ...
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bioRxiv - Cell Biology 2022Quote: ... 5 × 106 MCF-7 cells stably expressed shCtrl and shMYSM1 lentivirus were suspended in 100 μl of culture medium/Matrigel (1:1) (BD Biosciences) and implanted subcutaneously into the bilateral armpits of 4-week-old female BALB/c nude mice (Vital River Laboratory) ...
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bioRxiv - Developmental Biology 2023Quote: ... or (ii) PE-Annexin V conjugated antibody (1:33 dilution in TBST) and 7-AAD as per kit instructions (BD Pharmingen). Samples were analysed by flow cytometry within 30 min.
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bioRxiv - Immunology 2023Quote: ... from naïve/PBS-treated or i.n fusion protein immunized mice were isolated from mLN from a pool of 7-8 C57Bl/6 mice and FACS-sorted on a Aria II cell sorter (BD Bioscience). Labeling of cells prior to sorting by flowcytometry (Fusion ...
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bioRxiv - Cancer Biology 2023Quote: ... and total DNA (7-AAD 1:50 solution dilution) and analyzed by flow cytometer in a BD LSRFortessa cell analyzer (BD Biosciences) using 488 nm and 640 nm lasers ...
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bioRxiv - Biochemistry 2023Quote: ... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.