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Citations for Becton, Dickinson and Company :
301 - 350 of 5601 citations for 7 Amino 1 chloronaphthalene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the splenocytes were stained with the extracellular markers PE-Cy ™ 7 Hamster Anti-Mouse CD3e (Cat. # 552774, BD Pharmingen), APC-Cy7 Rat Anti-Mouse CD4 (Cat ...
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bioRxiv - Genetics 2020Quote: ... we also collected 5-7 ml of peripheral blood in a tube containing the anticoagulant K3 EDTA (#6457, BD Biosciences) which was used for flow cytometry as described below ...
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bioRxiv - Genomics 2021Quote: Each strain was cultivated for 3-7 days in 10 mL of Tryptic Soy broth (BD Diagnostic, catalog no. 211768) with 0.5% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-F4/80-PE clone REA126 and human anti-CD14-FITC from Miltenyi Biotech and 7-AAD staining solution from BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... The cell viability dye 7-AAD (Via-Probe) and Live/dead fixable Aqua fluorescent reactive dye were purchased from BD Biosciences and ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Images were analysed using Cellprofiler80 with default settings to quantify the cytoplasmic to nuclear intensity ratios.The following antibodies were used for IF: p53 (DO-7 FITC conjugate, BD biosciences), USP28 (Bethyl A300-898A) ...
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bioRxiv - Cell Biology 2022Quote: ... cultured PAECs were incubated with 2’,7’–dichlorofluorescin diacetate (DCFDA; 1μM) for 10 minutes at 37°C and then assessed by flow cytometry (BD LSRFortessa).
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bioRxiv - Immunology 2022Quote: ... HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml, BD Biosciences) and CD28 (1 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... at which point they were phenotyped (FOXP3-PeCy7, clone FJK-16s, Thermo Fisher Scientific; HELIOS-PEDazzle594, clone 22F6, Biolegend; Thy1.1-BV711, clone OX-7, BD Biosceinces ...
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bioRxiv - Cancer Biology 2024Quote: NF1-proficient and NF1-deficient engineered clones were injected into the flanks of 6- to 7-week-old athymic nude mice (1.0×106 cells) in 50% Matrigel (BD Biosciences). Mice were monitored for tumor formation for 6 months post injection or until a tumor volume of 2000mm3 was reached.
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bioRxiv - Bioengineering 2023Quote: ... Mice sera were collected retro-orbitally at 4 h and 7 days post administration and subjected to cytokine analysis (Cytometric Bead Array Mouse Inflammation Kit, BD) using flow cytometry following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were resuspended in ice cold PBS and supplied with 10 μl of 7-AAD to assess cell viability (BD Pharmigen) and sorted with a BD Aria III FACS as previously described 30 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Adherent and suspension cells of each sample were pooled and after centrifugation for 5min at 800 x g cells were carefully resuspended in cell culture medium with 10 µl/ml 7-AAD (BD Biosciences). After 10 minutes incubation in the dark cells were immediately analyzed by flow cytometry using a FACS Aria III (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... washed twice and resuspended in 200 μL of blocking buffer in the presence of 7-AAD (BD Biosciences Cat#: 51-68981E) or DAPI (Invitrogen Cat# ...
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bioRxiv - Cell Biology 2022Quote: ... Cell survival was measured by FACS analysis after staining cells with a membrane-impermeable DNA binding dye (DAPI, PI, or 7-AAD) and/or annexin V (BD Pharmingen).
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bioRxiv - Developmental Biology 2022Quote: ... Cells from mouse tail skin (aging RNA-seq) and dorsal/ventral skin (fibulin 7 RNA-seq) were isolated using FACS Aria flow cytometer (BD Biosciences). Data were analyzed using FlowJo software (BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... Myoblasts were stained with 7-AAD (cell viability) and with the positive selection marker anti-CD56 AF647-conjugated (clone R19-760, BD biosciences). Of note ...
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bioRxiv - Immunology 2020Quote: ... The cells were then stained with 7-AAD (live/dead marker) prior to flow cytometric sorting on an FACS Aria II sorter (BD Biosciences). The sorted germinal centre B cells (7-AAD−/B220+/GL-7+/Fas+ ...
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bioRxiv - Immunology 2021Quote: ... and then labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, FITC anti-mouse CD4, APC-Cy®7 mouse anti-CD8, all from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: Mononuclear cells were directly labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, clone, FITC anti-mouse CD4, APC-Cy®7 anti-mouse CD8, for T lymphocyte phenotype, all from BD Biosciences ...
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bioRxiv - Genomics 2020Quote: ... ODInCas9 system was left to turn off for 7 days post transfection before assessment using FACS instrument BD LSRFortessa (BD Bioscience) and confocal microscopy instrument Cell Voyager 7000S (CV7000S ...
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bioRxiv - Immunology 2019Quote: ... NK92 cells or primary patient fibroblasts were incubated with BrdU for 12 hours prior to fixation and staining with 7-AAD (BD Biosciences). Cells were analyzed via FACS on a BD Fortessa with 18-color configuration and resulting data were evaluated using Kaluza (Beckman-Coulter ...
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bioRxiv - Microbiology 2021Quote: ... The infectivity was measured by detecting GFP fluorescence using a Zeiss fluorescence microscope (AXIO observer 7) and measured as the percentage of GFP positive cells analyzed with a Fortessa X20 flow cytometer (BD Biosciences) at 24 hours post infection ...
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bioRxiv - Immunology 2020Quote: ... then washed with 2% FCS - PBS and stained with Mouse anti-IRF-7-PE (pS477/pS479, clone K47-671; BD Biosciences) or Mouse anti-NF-κB p65-Alexa Fluor 647 (pS529 ...
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bioRxiv - Cell Biology 2021Quote: ... macrophages were incubated with 5 μM 2’, 7’ dichlorofluorescein diacetate (DCFDA, Invitrogen at 37°C for 30 min, followed by acquisition using FACS Canto I (BD Sciences) within 30 minutes of staining ...
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bioRxiv - Cell Biology 2019Quote: ... Cells with normal mCherry fluorescence (mid 70-80% fluorescence) and top or bottom ~7% eGFP fluorescence (~4 million cells/population) were sorted using a BD FACSAria III (BD Biosciences) and collected into 15 ml polypropylene tubes (Cellstar ...
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bioRxiv - Immunology 2020Quote: ... Spleen B cells were analyzed for BrdU incorporation (by anti-BrdU mAb staining) and DNA contents (by 7–AAD staining after cells were permeabilized) using the APC BrdU Flow kit® (BD) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Infectivity was assessed by detecting GFP fluorescence using a Zeiss fluorescence microscope (AXIO observer 7) and/or determined by the percentage of GFP(+) cells analyzed using a Fortessa X20 flow cytometer (BD Biosciences) at 16 ...
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bioRxiv - Immunology 2022Quote: ... Human CD34+ stem cell purity was monitored by anti-human CD34 FITC (clone:581) and 7-AAD viability dye using LSR Fortessa X-20 flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 7 days and immunostained for flow cytometry (Antibodies: CD34, clone 581; CD117, clone: 104D2; CD11b, clone ICRF44 (BD Pharmingen) and 1:50 FcR Blocking Reagent (Miltenyi Biotec)) ...
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bioRxiv - Biochemistry 2023Quote: Cells were grown in synthetic complete medium at 25°C to mid-log phase after 7-8 doublings and harvested for direct fluorescent measurement of RFP and YFP channels by an LSR Fortessa (BD biosciences) flow cytometer ...
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bioRxiv - Cancer Biology 2023Quote: Apoptosis was measured by evaluating the activation of caspase 3/7 using the Ac-DEVD-AMC Caspase-3 Fluorogenic Substrate (BD Biosciences) according to manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Percentage of caspase-3/7 negative cells in each treatment condition was normalized to vehicle-treated control populations (FlowJo software v9, BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... from naïve/PBS-treated or i.n fusion protein immunized mice were isolated from mLN from a pool of 7-8 C57Bl/6 mice and FACS-sorted on a Aria II cell sorter (BD Bioscience). Labeling of cells prior to sorting by flowcytometry (Fusion ...
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bioRxiv - Biochemistry 2023Quote: ... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.
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bioRxiv - Microbiology 2023Quote: Bacteria used in this work are listed in Supplementary Table 7 and were grown at 37 °C in brain and heart infusion (BHI) broth (with agitation) and agar media (BD Difco). Cell lines of human origin used in this work included JEG-3 trophoblasts (ATCC HTB-36) ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclear extract was prepared by dounce homogenization in cold swelling buffer (using a size 7 dounce) and filtered using the cell strainer (100 μm, BD Biosciences). Lysate was centrifuged at 400 g for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Cell apoptosis was measured by co-staining cells with Annexin V and 7-AAD using the PE Annexin V Apoptosis Detection Kit I (BD Biosciences), according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... OPs were obtained by immuno-panning from single-cell suspensions of dissociated P6-7 mouse cortices using anti-PDGFRA immunoglobulin (558774, BD Pharmingen) as previously described (Emery and Dugas ...
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bioRxiv - Microbiology 2021Quote: ... and several ten-fold dilutions (10−4 to 10−7) were plated onto de Man Rogosa and Sharpe (MRS) media (BD Difco, USA) supplemented with 50 mg/L of mupirocin (PanReac AppliChem ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... and CD19 and the viability dye 7-aminoactinomycin-D (catalog #s 340662, 641407, 340692, 341103, 559925, respectively, BD Biosciences, San Jose, CA). The gating strategy is shown in Figure S1 ...
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bioRxiv - Developmental Biology 2022Quote: ... and cell cycle analysis was performed by staining cells with BrdU-APC and 7-AAD with BD Pharmingen™ APC BrdU Flow Kits (552598, BD Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 7 and 14) or at 2 time points (days 1and 14) after vaccination into Vacutainer® Heparin tubes (BD Biosciences, Plymouth, U.K.). Blood samples were subjected to immediate preparation and analyses ...
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bioRxiv - Neuroscience 2021Quote: ... PCA on MELAS versus control samples was performed on data from 7 MEA parameters (MFR, PRS, BR, BD, BSR, IBI, and NBR), since many samples did not show network bursts (i.e ...
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bioRxiv - Immunology 2020Quote: ... All antigen specific CD8 T cells were phenotypically analyzed by Flow Cytometry after surface staining using monoclonal antibodies for Thy1.1(BD clone OX-7), CD45.1 (Biolegend clone A20) ...
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bioRxiv - Microbiology 2020Quote: ... The fundamental M9 media formulation (pH 7) was supplemented with 0.8% deferrated acid-hydrolyzed casamino acids (BD Biosciences San Jose, CA, USA), 8 mM MgSO4 ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and centrifuged at 1000rpm for 5-10min and stained with APC-Cy™7 Rat Anti-Mouse CD3 (clone: 17A2, Cat # 560590, BD Biosciences), FITC Rat Anti-Mouse CD4 (Clone ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C in a TC-7 roller drum (NB, Canada) at 240 rpm or on Lysogeny Broth (LB; BD Difco, Canada) agar plates ...