Labshake search
Citations for Becton, Dickinson and Company :
301 - 350 of 362 citations for 6 Heptenal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Electroporated cells were cultured in 24-well plates for 5-7 days and transferred to 6-well plates prior to selection by fluorescence-activated single cell sorting using FACS Melody (BD Biosciences).
-
bioRxiv - Cell Biology 2024Quote: ... Cleaned coverslips were removed from 70% ethanol and allowed to dry in a biosafety cabinet then were placed into the wells of a 6 well tissue culture plate and coated with 10 µg/ml fibronectin (BD Biosciences) in PBS (10.1 mM Sodium phosphate dibasic ...
-
bioRxiv - Plant Biology 2023Quote: ... The flowers and the fixing solution were removed and replaced with 1 µg/mL DAPI (4′,6-diamidino-2-phenylindole, BD Biosciences) diluted in pollen isolation buffer before visualizing under a fluorescence microscope using a 420 nm filter (Zeiss LSM880 Inverted Confocal Microscope) ...
-
bioRxiv - Biophysics 2019Quote: Fresh peripheral blood samples were collected by venipuncture into 6 ml lithium-heparinized Vacutainer® tubes (BD Vacutainer™, Franklin Lakes, NJ) from healthy female and male donors with informed consent as approved by the Columbia University Medical Center Institutional Review Board (IRB protocol no ...
-
bioRxiv - Biochemistry 2019Quote: ... After 24 h cells were resuspended in enzyme-free EDTA buffer and replated in untreated 6-well plates (BD Falcon, Sigma-Aldrich) at 1 M cells per well in unconditioned medium ...
-
bioRxiv - Cancer Biology 2021Quote: Cells were grown on 6-well plates until 80-90% confluency in presence or absence of 10 μg/ml 9EG7 antibody (BD Biosciences, #553715) for 24 h and scraped off manually with a rubber Corning Cell Lifter (Corning ...
-
bioRxiv - Microbiology 2020Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were labeled with murine antibodies against CD3 (BV650) ...
-
bioRxiv - Microbiology 2020Quote: ... The sorted GFP− cells were stimulated with 10 ng/mL TNF-α for 6 h and then GFP expression levels were analyzed using BD FACSVerse (BD Biosciences). The level of gag expression after 18-h stimulation with 10 ng/mL TNF-α was analyzed by reverse-transcription (RT)-digital droplet PCR (ddPCR) ...
-
bioRxiv - Systems Biology 2022Quote: Approximately 6 mL of whole blood was collected at arrival into K3-EDTA glass blood tubes (BD Vacutainer; Franklin Lakes, NJ, USA) via jugular venipuncture ...
-
bioRxiv - Cancer Biology 2020Quote: Plasma serum was collected from 4-6 drops of peripheral blood obtained from NSGS mice via submandibular bleeding using Microtainer Collection Tubes (BD Microtainer, # 365967). Concentrations of 29 cytokines/chemokines were measured in 2-4 biological replicates using MILLIPLEX Map Human Cytokine/Chemokine Magnetic Bead Panel (HCYTMAG-60K-PX29 ...
-
bioRxiv - Immunology 2021Quote: ... Supernatants from cultured DCs were tested for the production of IL-10, IL-12p70 (eBioscience, USA) and IL-6 (BD Pharmingen, USA) by sandwich ELISA.
-
bioRxiv - Physiology 2019Quote: ... 10 mL blood was sampled from an antecubital forearm vein and 6 mL was dispensed into ethylenediaminetetraacetic acid-coated tubes (BD, Oxford, UK) and centrifuged (4°C at 3500 rpm ...
-
bioRxiv - Immunology 2021Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 4 h of incubation ...
-
bioRxiv - Immunology 2021Quote: ... The supernatant was collected after 6 days of co-culture to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
-
bioRxiv - Immunology 2021Quote: ... We used the following 28 antibodies::fluorophore conjugates and clones to enumerate major immune lineages using the Cytek Aurora: HLA-DR::BUV395 (BD; G46-6), CD8::BUV496 (BD ...
-
bioRxiv - Immunology 2020Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop™, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
-
bioRxiv - Cell Biology 2022Quote: ... Pelleted cells were incubated with anti-GFP-AF647 antibody (1:150, Alexa Fluor 647 Mouse Anti-GFP Clone 1A12-6-18, BD Biosciences 565197) or rabbit anti-ITGA5 (1:100 ...
-
bioRxiv - Immunology 2022Quote: ... samples were measured by a MACSQuant 18 (Figure 5) or 16 (Supplementary Figure 6) flow cytometer and analysed by FlowJo® software (BD company). Three spleen samples from lmon_0149 vaccinated mice (Figure 5 ...
-
bioRxiv - Physiology 2022Quote: ... Venous peripheral blood (4-6 ml for adults, 1- 3 mL for children and infants) was collected on-site on EDTA (BD vacutainer©) and immediately stored at 4-8°C until use ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Cells were then washed with PBS+5% FBS and stained with 1:100-dilutions of AF647-conjugated anti-PAX-6 (BD Biosciences; 562249) and PerCP-Cy5.5-conjugated anti-SOX-2 (BD Biosciences ...
-
bioRxiv - Immunology 2023Quote: The plate was incubated 6 hours at 37 °C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 4 hours of culture ...
-
bioRxiv - Cell Biology 2023Quote: ... plated at a density of 1 X 106 cells/well in a Matrigel-coated 6 well plates (BD, 354277; Fisher Scientific, 140675). For the first 24 hours after passaging ...
-
bioRxiv - Immunology 2023Quote: ... T cells and RP KD tumor cells were then co-cultured for 6 hours in the presence of GolgiPlug (BD Biosciences 555029) and GolgiStop (BD Biosciences 554724 ...
-
bioRxiv - Microbiology 2024Quote: ... and HIV-1 NL4-3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
-
bioRxiv - Microbiology 2024Quote: ... as well as HIV-1 NL4.3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
-
bioRxiv - Immunology 2021Quote: Eosinophils of 6-12 week-old Il5-tg females and males were positivelyenriched using PE anti-mouse Siglec F antibody (562068 BD Biosciences; E50-2440) and anti-PE microbeads (130-042-401 Miltenyi Biotech) ...
-
bioRxiv - Physiology 2019Quote: Liver tissues were weighed and homogenized in assay diluent provided by the TNF-α or IL-6 ELISA kit (BD, Franklin Lakes, NJ). Plasma samples were collected with heparin as anticoagulant ...
-
bioRxiv - Physiology 2019Quote: Primary hepatocytes were plated on 8-well chamber slides for immunofluorescence imaging and on collagen-coated 6-well plates (BD, Franklin Lakes, NJ) for PI/Hoechst stains and MitoSox stains ...
-
bioRxiv - Cancer Biology 2020Quote: ... 6.6×105 starved MDAMB231 cells expressing the shRNA library (or control cell line) were plated onto 6-well transwell chambers (8 μm pore size membrane; BD Bioscience, Bedford, MA) in assay medium ...
-
bioRxiv - Cancer Biology 2020Quote: ... 6.6×105 starved MDA-MB-231 cells expressing the shRNA library (or control cell line) were plated onto 6-well Boyden chambers (8 μm pore size membrane; BD Bioscience, Bedford, MA) in assay medium ...
-
bioRxiv - Cell Biology 2019Quote: ... the plasma levels of pro-inflammatory cytokines IL-6 and TNFα were quantified by bead-based flow cytometry assay (CBA Kit; BD Biosciences, Heidelberg, Germany) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2020Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
-
bioRxiv - Immunology 2021Quote: Absolute numbers of B cells and T cells in peripheral blood were measured using BD Multitest™ 6-color TBNK reagents and BD Trucount™ Tubes (BD Biosciences) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... The cell mixture was allowed to incubate for 6 hours in the presence of anti-CD28/Ly29d Abs (BD Biosciences, San Jose, CA), brefeldin A at a final concentration of 10 mg/ml (Sigma-Aldrich) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... One mL of such a mix was then used to fertilize approximately 24 eggs of a female in wells of 6-well plates (Falcon, BD Biosciences, Allschwil, Switzerland). This 1 mL of mix was activated in a separated tube with either 4 mL of chemically standardized water (i.e ...
-
bioRxiv - Immunology 2023Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
-
bioRxiv - Cancer Biology 2023Quote: A total of 1.0 × 106 NMuMG-Venus cells and NMuMG-HOXB7 cells were transplanted into 6-8-weeks-old Rag2-/- female mice with 100 μL 50% Matrigel (BD Bioscience, CA, USA) as previously described (Ihara et al ...
-
bioRxiv - Neuroscience 2020Quote: ... and plated as dissociated cells (25 × 104 cells/well) on day −2 in a 6-well Matrigel-coated plate (BD Biosciences, San Jose, CA). On day −1 ...
-
bioRxiv - Cancer Biology 2022Quote: ... The co-cultures were incubated for 6 hours at 37°C and 5% CO2 prior to cell staining using the Cytofix/Cytoperm Fixation Kit (BD Biosciences, Cat# 554715/555028).
-
bioRxiv - Neuroscience 2021Quote: ... Stimulated cells were stained with fluorochrome-conjugated anti-MCP-1 and anti-IL-6 Abs using the Cytofix/Cytoperm kit (BD Biosciences, San Jose, CA) to determine MCP-1 and IL-6 production ...
-
bioRxiv - Immunology 2021Quote: ... Single leucocyte suspensions were stimulated with either phorbol 12-myristate 13-acetate (PMA)-ionomycin or 5 µg/ml of MCMV peptide pool (Table S3) for 6 hours with brefeldin A (BD Biosciences, San Jose, CA). After stimulation ...
-
bioRxiv - Neuroscience 2020Quote: ... cells were examined in the CyFlow® Cube 6 (Sysmex America, United States) and data was analyzed using the FlowJo™ v10.7 software (BD biosciences, United States).
-
bioRxiv - Neuroscience 2021Quote: ... using BD CBA Flex Sets (GCSF: #560152, KC: #558340, IL-1α: #560157, IL-1β: #560232, IL-6: #558301, TNF-α: #558299, BD, Becton, Dickinson and Company) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: Blood was collected from the saphenous vein at baseline (week 0) and at 3 and 6 weeks in microtainer serum separator tubes (BD Biosciences, Mississauga, ON, Canada). Cleared serum samples were obtained following the manufacturer’s protocol and stored at −20°C until used ...
-
bioRxiv - Microbiology 2021Quote: ... coli was suspended in 1 ml of spent supernatant to 0.05 OD600 and inoculated atop solid LB agar in a 6-well plate (BD Biosciences Inc., Franklin Lakes, NJ).
-
bioRxiv - Immunology 2021Quote: ... The stimulation was performed by incubation for 6 h at 37°C and 5% CO2 in the presence of Protein Transport Inhibitor Cocktail (brefeldin A) (BD Biosciences, San Jose, CA) at a final concentration of 1:1000 ...
-
bioRxiv - Immunology 2022Quote: The levels of cytokines in cell culture medium were determined with BD OptEIA™ Mouse IL-6 ELISA set (BD Biosciences, Franklin Lakes, NJ), ELISA MAX™ Standard Set Mouse IL-10 (BioLegend) ...
-
bioRxiv - Microbiology 2023Quote: ... incubated with 60 nM TMRE for 30 min at 37 °C, washed in PBS, resuspended in PBS-G (PBS, 6 mM glucose) and immediately analyzed by flow cytometry (BD FACS Canto II Instrument). In the case of oligomycin-treated cells ...
-
bioRxiv - Microbiology 2023Quote: ... The cytokine releases were evaluated by ELISA in cell supernatants or in peritoneal fluids (IL-1β, IL-12, IL-6, TNFα, TGFβ, IL-10 BD Biosciences, R&D Systems). 8 isoprostane ...
-
bioRxiv - Immunology 2019Quote: ... Serum was collected by tail-tipping before LPS injection and 2 and 4 hours following injection for IL6 determination by ELISA (BD; OptEIATM Set Mouse IL-6), as per manufactures instructions.