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Citations for Becton, Dickinson and Company :
601 - 650 of 1512 citations for 6 Fluoro 2 methylnicotinaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... cells were plated at 1/6 confluence in either six 175 cm2 flasks or in two three-layer 525 cm2 tissue culture flasks (BD Biosciences) and after 24 hours synchronized at different stages of the cell cycle as described in the previous section ...
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bioRxiv - Cancer Biology 2019Quote: ... the cells were re-stimulated for another 6 h with the anti-CD3 antibody in the presence of both GolgiStop and GolgiPlug (BD Biosciences) and stained with antibodies against IFN-γ and TNF-α ...
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted by centrifugation at 600 x g for 6 minutes and fixed in 100 μL per 1×106 cells of PhosFlow Lyse/Fix buffer (BD Bioscience) at 37 °C for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... and the remaining cells were incubated for 5-6 hrs at 37°C in the presence of 1 uM of the indicated peptide and brefeldin A (GolgiPlug; BD Pharmingen). Surface staining was done overnight at 4 degrees ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 5-6 days and CFSE dilution of T cells was determined on a BD Canto II (BD Bioscience) and analyzed by FlowJo (v10.1 ...
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bioRxiv - Immunology 2020Quote: ... fibroblast-collagen cultures were prepared in Falcon cell culture inserts and placed into Falcon 6 Well Deep Well TC-Treated Polystyrene Plates (BD Biosciences) containing 2×105 fibroblasts in FBS ...
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bioRxiv - Immunology 2021Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Tracheas were stretched out by pinning to SylGard-coated well of 6 well plate and fixed for 1 hr on ice in Cytofix/Cytoperm buffer (BD Biosciences). Immunofluorescent staining was performed in PBS with 0.25% Triton X-100 at 4°C as follows ...
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bioRxiv - Immunology 2022Quote: ... and 10% heat-inactivated FBS) and incubated at 37°C for 6 hours with protein transport inhibitors GolgiStop and GolgiPlug (BD Biosciences) and S glycoprotein peptide pools 1 μg/mL (JPT product PM WCPV-S-1 ...
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bioRxiv - Microbiology 2020Quote: ... from the four subgroups were co-cultured in the Biolog AN Microplate in a pairwise fashion (n =6, AB, AC, AD, BC, BD, CD), by combining 50 μL of each isolate suspended in inoculation fluid in each well ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s protocol and intracellular staining was performed using anti-BCL-6-AF647 (clone K112-91, 1:100, BD, cat#561525).
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bioRxiv - Immunology 2019Quote: Emigrated cells were washed in PBS with 1% BSA and 0.02% NaN3 and incubated for 30 minutes on 4°C with subset markers HLA-DR BV510 (clone G46-6, BD biosciences), CD1a APC (clone HI149 ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293 cells transfected with ABE plasmids were collected from 6-well plate and analyzed on Becton Dickinson LSR II (BD Biosciences) to determine GFP-positive cells ...
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bioRxiv - Cell Biology 2020Quote: ... the cell surface of freshly-prepared epidermal cells was labeled with the following antihuman protein antibodies (clone, manufacturer): FITC–conjugated CD1a (HI149) and PE–conjugated HLA-DR (G46-6, both BD Pharmingen), PE–CF594–conjugated CD3 (UCHT1 ...
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bioRxiv - Immunology 2021Quote: ... and circulating plasmablasts from 6 donor mice per group (ApoE-/- versus ApoE-/-Gpr55-/- mice) were sorted using a BD FACSAria III Cell Sorter (BD Biosciences) as described above and bulk RNA-sequencing was performed using the prime-seq protocol.26 A step-by-step protocol can be found on protocols.io (dx.doi.org/10.17504/protocols.io.s9veh66) ...
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bioRxiv - Pathology 2021Quote: ... 0.2 micron sterile filtered) using an insulin syringe (31-gauge, 6 mm length, 3/10 cc, BD Veo Ultra-Fine needle). Glucose and lactate levels were measured using a minimally invasive tail-prick method at designated timepoints post-injection.
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bioRxiv - Neuroscience 2021Quote: ... The suspended cell lysates were pipetted 6 times gently and passed through a syringe 20 times (1 ml TB Syringe, BD, USA), and avoid any bubble in the procedures ...
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bioRxiv - Microbiology 2021Quote: ... The lung infiltrated cells were cultured with purified RBD protein at 10 μg/ml for 12 h at 37 °C followed by a 6 h treatment with GolgiPlug (BD 555028). After blocking with FcγIII and FcγII receptors antibody (BD Pharmingen ...
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bioRxiv - Immunology 2021Quote: Cells were co-cultured in R10 medium alone or in presence of K562 cells for 6 hours in the presence of anti-human CD107a (H4A3, FITC, BD Biosciences). Golgi plug and Golgi stop (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... the endothelial cell monolayers in 6-well plates were lysed after removing center region using cell scraper (BD Falcon #35-3085) and washing with 1X HBSS (Corning #21-022-CV).
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bioRxiv - Neuroscience 2023Quote: ... single viable cells were gated and CD45highCD8+ cells were collected using a FACSAria III and corresponding software (FACSDiva, v.6; BD Biosciences). Calculation of the number of CD45highCD8+ T cells per brain was performed by extrapolating their frequency to the counted total number of isolated cells ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were recovered and the amounts of IL-6 were analyzed by cytokine-specific ELISA kits (BD Biosciences, Mountain View, CA).
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bioRxiv - Bioengineering 2023Quote: ... Circulating levels of IL-6 and TNF were determined using a cytokine bead array (BDTM Cytometric Bead Array Mouse IL-6/TNF Flex Set, BD Biosciences). Samples were analyzed in technical triplicates.
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bioRxiv - Neuroscience 2023Quote: ... single viable cells were gated and CD45-O1+ and CD45lowSiglec- H+ cells were collected using a FACSAria III and corresponding software (FACSDiva, v.6; BD Biosciences). Calculation of the number of CD45lowSiglecH+ microglia per brain was performed by extrapolating their frequency to the counted total number of isolated cells ...
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bioRxiv - Microbiology 2023Quote: Stm strain SL1344 with plasmid pMW211[C.10E/DsRed] [20] and strain 12023 with plasmid pluxCDABE [6] were recovered from frozen glycerol stock and cultured in Difco Luria-Bertani (LB) Broth (BD Biosciences) containing 100 µg/ml ampicillin (Merck ...
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bioRxiv - Immunology 2023Quote: ... from naïve/PBS-treated or i.n fusion protein immunized mice were isolated from mLN from a pool of 7-8 C57Bl/6 mice and FACS-sorted on a Aria II cell sorter (BD Bioscience). Labeling of cells prior to sorting by flowcytometry (Fusion ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then stained for intracellular IL-6 and events were collected using the BD LSR Fortessa cell analyzer (BD Biosciences). Data was analyzed using the FlowJo Software (BD Biosciences).
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bioRxiv - Immunology 2023Quote: Non-pregnant Foxp3eGFP and C57BL/6 females were used to isolate peripheral CD4+ or Treg cells by cell sorting using FACS ARIA (BD Biosciences). Total lymph nodes and spleens were dilacerated ...
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bioRxiv - Cancer Biology 2023Quote: ... a single cell suspension was generated using trypsin and the cells were sorted based on GFP intensity into 6 equally logged bins using BD FACS Aria III (BD Biosciences). In a parallel control condition ...
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bioRxiv - Genetics 2023Quote: ... we sorted up to 6 million cells for each FlowFISH replicate into 6 bins based on the fluorescence intensity of the target gene using the Influx Cell Sorter (BD Biosciences). To control for differences in staining efficiency of each cell ...
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bioRxiv - Immunology 2023Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... we resuspended the cells in 0.6 ml of cold PBS + RNase inhibitor and then filtered the cells with 100-μm cell strainers (Falcon brand, BD Biosciences). Cells were then incubated with fluorophore-conjugated primary antibodies against NeuN and Fos ...
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bioRxiv - Cell Biology 2024Quote: ... Electroporated cells were cultured in 24-well plates for 5-7 days and transferred to 6-well plates prior to selection by fluorescence-activated single cell sorting using FACS Melody (BD Biosciences).
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bioRxiv - Cell Biology 2024Quote: ... Cleaned coverslips were removed from 70% ethanol and allowed to dry in a biosafety cabinet then were placed into the wells of a 6 well tissue culture plate and coated with 10 µg/ml fibronectin (BD Biosciences) in PBS (10.1 mM Sodium phosphate dibasic ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... ELISA was performed to measure IL-2 concentration of the thawed samples by using mouse IL-2 ELISA set (BD Biosciences, Franklin Lakes, NJ, USA) according to manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... mice were anesthetized with isoflurane and xylazine (2%, 2 mg/kg) then blood from vena cava was collected into lithium heparin-coated tubes (BD Microtainer, Franklin Lake, NJ, USA). Plasma was prepared by centrifugation (1500 g ...
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bioRxiv - Biophysics 2019Quote: Fresh peripheral blood samples were collected by venipuncture into 6 ml lithium-heparinized Vacutainer® tubes (BD Vacutainer™, Franklin Lakes, NJ) from healthy female and male donors with informed consent as approved by the Columbia University Medical Center Institutional Review Board (IRB protocol no ...
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bioRxiv - Biochemistry 2019Quote: ... After 24 h cells were resuspended in enzyme-free EDTA buffer and replated in untreated 6-well plates (BD Falcon, Sigma-Aldrich) at 1 M cells per well in unconditioned medium ...
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bioRxiv - Cancer Biology 2021Quote: Cells were grown on 6-well plates until 80-90% confluency in presence or absence of 10 μg/ml 9EG7 antibody (BD Biosciences, #553715) for 24 h and scraped off manually with a rubber Corning Cell Lifter (Corning ...
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bioRxiv - Microbiology 2020Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were labeled with murine antibodies against CD3 (BV650) ...
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bioRxiv - Microbiology 2020Quote: ... The sorted GFP− cells were stimulated with 10 ng/mL TNF-α for 6 h and then GFP expression levels were analyzed using BD FACSVerse (BD Biosciences). The level of gag expression after 18-h stimulation with 10 ng/mL TNF-α was analyzed by reverse-transcription (RT)-digital droplet PCR (ddPCR) ...
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bioRxiv - Systems Biology 2022Quote: Approximately 6 mL of whole blood was collected at arrival into K3-EDTA glass blood tubes (BD Vacutainer; Franklin Lakes, NJ, USA) via jugular venipuncture ...
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bioRxiv - Cancer Biology 2020Quote: Plasma serum was collected from 4-6 drops of peripheral blood obtained from NSGS mice via submandibular bleeding using Microtainer Collection Tubes (BD Microtainer, # 365967). Concentrations of 29 cytokines/chemokines were measured in 2-4 biological replicates using MILLIPLEX Map Human Cytokine/Chemokine Magnetic Bead Panel (HCYTMAG-60K-PX29 ...
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bioRxiv - Immunology 2021Quote: ... Supernatants from cultured DCs were tested for the production of IL-10, IL-12p70 (eBioscience, USA) and IL-6 (BD Pharmingen, USA) by sandwich ELISA.
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bioRxiv - Physiology 2019Quote: ... 10 mL blood was sampled from an antecubital forearm vein and 6 mL was dispensed into ethylenediaminetetraacetic acid-coated tubes (BD, Oxford, UK) and centrifuged (4°C at 3500 rpm ...
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bioRxiv - Immunology 2021Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 4 h of incubation ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected after 6 days of co-culture to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... We used the following 28 antibodies::fluorophore conjugates and clones to enumerate major immune lineages using the Cytek Aurora: HLA-DR::BUV395 (BD; G46-6), CD8::BUV496 (BD ...
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bioRxiv - Immunology 2020Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop™, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...