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Citations for Becton, Dickinson and Company :
301 - 350 of 438 citations for 6 9 Diaza spiro 4.5 decane 2HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... PFB-FDGlu (50 µg/mL) was added for 6 h and the nPFB-FDGlu fluorescence was measured by BD FACSAria™ III Cell Sorter (λex 492 nm and λem 516 nm) ...
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bioRxiv - Biochemistry 2024Quote: ... PFB-FDGlu (50 µg/mL) was added for 6 h and the nPFB-FDGlu fluorescence was measured by BD FACSAria™ III Cell Sorter (λex 492 nm and λem 516 nm).
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bioRxiv - Immunology 2019Quote: (DAPInegCD3negCD11cnegCD14negCD19negTCRβnegTCRγnegNK1.1negKLRG1negCD127+CD90.2+) were isolated from small intestine LPLs of C57BL/6 mice using the ARIA II FACS Sorter (BD Biosciences). ILC3 were cultured at 37°C in flat bottom 96 well plates (104 cells/well ...
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bioRxiv - Genetics 2019Quote: ... a second set of antibodies was used (volume per 1×10^6 cells, all from BD Biosciences, unless stated otherwise): CD45 V500 (4μl ...
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bioRxiv - Microbiology 2021Quote: ... The cells were resuspended with 200 μl of PBS and detected with a flow cytometer of Accuri 6 (BD Biosciences). The fluorescent labeled wheat germ agglutinin (WGA) ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were plated at a concentration of 150,000 cells per 6-well in mTeSR-1 with 10 uM Y27632 (BD Biosciences). The next day ...
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bioRxiv - Biochemistry 2020Quote: ... all-atom MD simulations in 150 mM aqueous solution (referred to as “soluble simulations”) were carried out for all 6 representative structures of CYP 1A1 □ CPR encounter complexes obtained from BD simulations ...
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bioRxiv - Microbiology 2020Quote: ... pH 5.2) and non-fermentative medium (glycerol 6%; YNB without amino acid and ammonium sulfate 0.017% (BD Difco™, U.S.); CSM 0.077% (MP Biomedicals™ ...
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bioRxiv - Cell Biology 2020Quote: ... ELISA kits were used to quantify secreted TNF (Ready-SET-Go! Mouse TNFα Kit, eBiosciences,88732488) and IL-6 (BD OptiEIA ...
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bioRxiv - Cell Biology 2020Quote: ... Tacc3fl/fl mice by flow cytometry (GFP or tdTomato) as previously described(6) using a FACSAriaII cell sorter (BD Biosciences), or alternatively from Pax7CreERT2/+ or Pax7CreERT2/+ ...
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bioRxiv - Cancer Biology 2022Quote: Wells with the highest proportion of target barcodes were revived into a 6 well plate and grown for 4 days before being single-cell sorted by BD FACSAria II into 96 well plate ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 1.0 x106 cells were added to the 6-wells plates and treated with anti-CD16/32 antibody (BD Biosciences) to block the Fc receptor ...
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bioRxiv - Immunology 2019Quote: Cells were analysed using 4- to 6-color direct immunofluorescence staining on a BD LSR II flow cytometer (BD Biosciences). Antibodies used were ...
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bioRxiv - Microbiology 2021Quote: ... CD21 BV786 (Clone B-LY4, #740969), HLA DR BB700 (Clone G46-6, #566480), CD27 APC R700 (Clone M-T271, #565116) all from BD Biosciences ...
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bioRxiv - Cell Biology 2019Quote: IL-6 is an important cytokine implicated in inflammation and is abundantly released by tendon stromal cells isolated from patients with shoulder tendon tears after stimulation with IL-1β15 IL-6 in tissue culture supernatants was measured using enzyme-linked immunosorbent assay (ELISA) reagents (BD Biosciences) using incubations isolated from 5 donors ...
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bioRxiv - Cancer Biology 2020Quote: ... pure and mixed cells were cultured in 6-well plates and FACS sorted 3 days after mixing on a FACS Jazz system (BD). After a FSC/SSC gate doublets were excluded ...
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bioRxiv - Microbiology 2020Quote: The concentration of IL-6 in serum of infected mice was determined by ELISA according to the manufacturer’s recommendations (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... wells were washed 6 times as before and HRP activity was detected using TMB (3,3’, 5,5’ tetramethylbenzidine) reagent (BD OptEIA™) according to the manufacturer’s instructions (incubating for 15 −30 min) ...
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bioRxiv - Cancer Biology 2020Quote: ... The following antibodies were used for the 3D cultures: alpha-6-integrin (BD Biosciences 25-0495-82, diluted 1:80), ZO-1 (Invitrogen 61-7300 ...
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bioRxiv - Cancer Biology 2020Quote: ... Flow cytometry analysis was performed before the beginning of the culture and at day 5 and 6⍰using LSRII (BD).
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bioRxiv - Immunology 2021Quote: Spleen and lymph nodes from C57BL/6 mice were surgically removed and single cells were obtained mechanically using a 70μm cell strainer (BD Falcon). Red blood cells were lysed by ACK lysis buffer (Lonza ...
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bioRxiv - Microbiology 2023Quote: The release of IL-10 and IL-6 were quantified in cell supernatants using specific immunoassay kits purchased from BD Biosciences ...
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bioRxiv - Immunology 2023Quote: CD44+CD62L+ and CD44-CD62L+ DN1 T cells were sorted from LNs of DN1-hCD1Tg BM chimera mice vaccinated with MA-BCN at 6 weeks post vaccination by BD FACS Aria with purity >98% ...
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bioRxiv - Immunology 2023Quote: ... approximately 4-6 × 106 isolated murine CD4+ T cells/well were stimulated in a 6-well plate pre-coated with 1μg/ml αCD3 (clone 145-2C11, BD Pharmingen) and 1μg/ml and αCD28 (clone 37.51 ...
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bioRxiv - Immunology 2023Quote: ... and 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) and analyzed on a BD FACS Aria III (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... were stimulated with peptide pools of ESAT-6 and CFP-10 (10μg/ml of each peptide pool) in the presence of Brefeldin A (GolgiPlug, BD Biosciences) at 37°C/5% CO2 for 3.5-4 hours prior to staining ...
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bioRxiv - Immunology 2024Quote: ... CD16 BV421 (clone 3G8, catalog #: 562878), HLA-DR BV786 (clone G46-6, catalog #: 564041), and CD15 BV510 (clone W6D3, catalog #: 563141) from BD Biosciences.
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bioRxiv - Immunology 2024Quote: ... T cells or NK cells were mixed with tumor cells for 6 h in the presence of GolgiPlug (BD, BDB555029). As a positive control ...
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bioRxiv - Microbiology 2024Quote: ... were selected for each sample (for a total of up to 6 colonies) and sub-streaked onto non-selective brain heart infusion agar plates (BHIA, BD), followed by incubation at 37°C for 48 h ...
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bioRxiv - Cancer Biology 2024Quote: ... with 4′,6-diamidino-2-phenylindole (DAPI) as a viability dye and the following antibodies: anti-CD31 PE (BD Biosciences), anti-CD45 APC (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: NF1-proficient and NF1-deficient engineered clones were injected into the flanks of 6- to 7-week-old athymic nude mice (1.0×106 cells) in 50% Matrigel (BD Biosciences). Mice were monitored for tumor formation for 6 months post injection or until a tumor volume of 2000mm3 was reached.
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bioRxiv - Cancer Biology 2024Quote: ... bone marrow aspirates were performed every 6 weeks post-transplant and human chimerism was tracked by flow cytometry analysis (using a BD LSR Fortessa analyzer or BD FACSymphony ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were co-stained with 4′,6-diamidino-2-phenylindole (DAPI) to measure DNA content and analyzed with a BD LSRFortessa flow cytometer (BD Biosciences) and FlowJo software (TreeStar).
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bioRxiv - Cell Biology 2020Quote: ... Percentage of platelets in BALF (n=3-6) was quantified on a BD FACSVerse flow cytometer (BD Biosciences, San Jose, USA) and analysed with FlowJo v10 software (FlowJo LLC ...
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bioRxiv - Immunology 2019Quote: ... The cecum was exteriorized and ligated with a 6.0 silk suture (6-0 PROLENE, 8680G; Ethicon) placed below the ileo-cecal valve and perforated with a 21-gauge needle (BD Biosciences) to induce mid-grade lethal sepsis78–80 ...
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bioRxiv - Immunology 2019Quote: ... 25 μL of cell suspension from each well was washed with 100 μL PBS-EDTA and stained with 5 μL of HLA-DR-FITC (clone G46-6, BD BioSciences) or isotype control (clone G155-178 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated at 1/6 confluence in either six 175 cm2 flasks or in two three-layer 525 cm2 tissue culture flasks (BD Biosciences) and after 24 hours synchronized at different stages of the cell cycle as described in the previous section ...
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bioRxiv - Microbiology 2020Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Cancer Biology 2019Quote: ... the cells were re-stimulated for another 6 h with the anti-CD3 antibody in the presence of both GolgiStop and GolgiPlug (BD Biosciences) and stained with antibodies against IFN-γ and TNF-α ...
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted by centrifugation at 600 x g for 6 minutes and fixed in 100 μL per 1×106 cells of PhosFlow Lyse/Fix buffer (BD Bioscience) at 37 °C for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... and the remaining cells were incubated for 5-6 hrs at 37°C in the presence of 1 uM of the indicated peptide and brefeldin A (GolgiPlug; BD Pharmingen). Surface staining was done overnight at 4 degrees ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 5-6 days and CFSE dilution of T cells was determined on a BD Canto II (BD Bioscience) and analyzed by FlowJo (v10.1 ...
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bioRxiv - Immunology 2020Quote: ... fibroblast-collagen cultures were prepared in Falcon cell culture inserts and placed into Falcon 6 Well Deep Well TC-Treated Polystyrene Plates (BD Biosciences) containing 2×105 fibroblasts in FBS ...
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bioRxiv - Immunology 2021Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Tracheas were stretched out by pinning to SylGard-coated well of 6 well plate and fixed for 1 hr on ice in Cytofix/Cytoperm buffer (BD Biosciences). Immunofluorescent staining was performed in PBS with 0.25% Triton X-100 at 4°C as follows ...
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bioRxiv - Immunology 2022Quote: ... and 10% heat-inactivated FBS) and incubated at 37°C for 6 hours with protein transport inhibitors GolgiStop and GolgiPlug (BD Biosciences) and S glycoprotein peptide pools 1 μg/mL (JPT product PM WCPV-S-1 ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 and MIP-2 were measured using paired antibodies and recombinant cytokines according to the manufacturer’s recommendations (BD PharMingen Systems) (11).
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bioRxiv - Microbiology 2020Quote: ... from the four subgroups were co-cultured in the Biolog AN Microplate in a pairwise fashion (n =6, AB, AC, AD, BC, BD, CD), by combining 50 μL of each isolate suspended in inoculation fluid in each well ...
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bioRxiv - Microbiology 2021Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s protocol and intracellular staining was performed using anti-BCL-6-AF647 (clone K112-91, 1:100, BD, cat#561525).