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Citations for Becton, Dickinson and Company :
1 - 50 of 198 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 2x AbSeq master mix of Immune Discovery Panel (BD Biosciences) prepared as the provider’s protocol was added to the cell suspension and stained for 15 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... prior to staining with a 2X master mix containing fluorophore-labeled antibodies at pre-titrated concentrations in Brilliant Violet Staining Buffer (BD Biosciences), and added to suspended cells at a final staining concentration of 2 x 108 cells/mL for 30-45 min ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated with AbSeq antibody master mix (BD Biosciences, table S3) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Master mix of surface markers was prepared in Brilliant Buffer solution (BD BioSciences) which consisted of anti-human Alexa Fluor® 700 anti-human CD3 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... an antibody master mix (Supplementary table 1) and staining buffer (Brilliant Stain Buffer, BD Biosciences) were used ...
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bioRxiv - Immunology 2021Quote: ... The 24-color extracellular staining master mix (Extended Data Table 2) was prepared 2X in BD Horizon™ Brilliant Stain Buffer to prevent staining artifacts from BD Horizon Brilliant dye interactions and added 1:1 to cells ...
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bioRxiv - Immunology 2020Quote: ... Then we added 1 mL of a second antibody master mix consisting of Brilliant Buffer (BD Biosciences) and 9 markers for four-way sorting (Supplementary Table 12) ...
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bioRxiv - Immunology 2020Quote: ... we resuspended in 50 uL of the first antibody master mix consisting of Brilliant Stain Buffer (BD Biosciences) and CCR6 - PE/Cy7 (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Then we added 50 uL of a second antibody master mix consisting of Brilliant Stain Buffer (BD Biosciences) and 11 surface markers (Supplementary Table 12) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with 50 µL of extracellular antibody master mix in Brilliant Stain Buffer (BD Bioscience) for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... samples were stained with SYBR Green I and three technical replicates were measured on an Accuri C6 (BD Biosciences). Flow cytometry data is available from FlowRepository (accession IDs ...
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bioRxiv - Microbiology 2020Quote: ... stained with SYBR Green I and two technical replicates per population were measured on an Accuri C6 (BD Biosciences). Fluorescence was measured by the targeted detector (FL1 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained with SYBR green I prior to analysis on a Becton Dickinson Influx Cell Sorter (BD ICS). The BD ICS was equipped with a 488 nm laser and detectors for forward scatter (FSC) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Lateral light scatter (SSC) and SYBR-green fluorescence (FITC) measurements were performed with logarithmic amplification using FlowJo software (BDBiosciences). Virus and virophage samples were immediately collected for DNA extraction and quantified by ddPCR (see above and (51)).
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bioRxiv - Immunology 2020Quote: ... we stained samples with an antibody master mix (Supplementary Table 4) in Brilliant Stain buffer (BD Bioscience, Cat #566349). We covered in foil and incubated for 25 min at 4° C ...
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bioRxiv - Microbiology 2021Quote: ... An aliquot of (100 μl) master mix sample was serially diluted using PBS and plated onto nutrient agar (BD, Difco). All plates were incubated at 30 °C corresponding to the river water temperature for 2 days ...
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bioRxiv - Microbiology 2020Quote: ... cell staining with SYBR® green and propidium iodide was performed after which cells were counted on an Accuri C6+ Flow cytometer from BD Biosciences Europe ...
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bioRxiv - Immunology 2021Quote: ... Single cells were index sorted into 384 well plates containing 1uL of reverse transcription (RT) master mix using a FACS Aria III with FACS Diva software (BD Biosciences) to maintain protein expression with plate well location ...
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bioRxiv - Immunology 2020Quote: We centrifuged the samples and resuspended each in 1 mL of an antibody master mix consisting of Brilliant Buffer (BD Biosciences) and anti-CCR6 - PECy7 (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Single cells were index sorted into 384 well plates containing 1uL of reverse transcription (RT) master mix using a FACS Aria III with FACS Diva software (BD Biosciences) to maintain protein expression with plate well location ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: 344SQ murine lung adenocarcinoma cells expressing firefly luciferase and green fluorescent protein (in 50 μL of 1:1 mix of HBSS and BD Matrigel) were injected into the left lung of 8 weeks old female 129/Sv mice via intrapulmonary injection as described previously (50) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 20 minutes at room temperature with 100 μl total volume of antibody master mix freshly prepared in Brilliant staining buffer (BD Bioscience, #563794), followed by two washes ...
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bioRxiv - Systems Biology 2021Quote: ... The supernatant was purified 2x using SPRIselect beads (BD), first at 0.6x and then at 0.8x the volume of cDNA and finally amplified in KAPA HiFi ready mix (Roche ...
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Simultaneous adjunctive treatment of malaria and its co-evolved genetic disorder sickle cell anaemiabioRxiv - Microbiology 2022Quote: ... These plates were screened for viable parasites after 14 to 21 days by staining 20 μl/well of samples with SYBR Green I and Mito Tracker Deep Red followed by flow cytometry (BD LSRFortessa X-20 flow cytometer) as described above ...
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bioRxiv - Genomics 2023Quote: ... 50 µl of SYBR Green I stained RBCs were analyzed on BD LSR Fortessa Flow Cytometer with High Throughput sampler (BD Biosciences, San Jose, CA, USA) using BD FACS Diva Software v6.2 and 488 laser excitation / 530 emission filter to determine the concentration of SYBR Green I positive cells per microliter ...
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bioRxiv - Systems Biology 2020Quote: ... SC limiting media were prepared from YNB without the relevant nutrient (Low Phosphate medium - ForMedium, CYN0804, Low Nitrogen medium - BD 3101130). Phosphate depleted medium was prepared by adding phosphate in the form of KH2PO4 to a final concentration of 0.2mM ...
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bioRxiv - Cancer Biology 2020Quote: ... Low-passage cells were seeded on Cell-Tak (BD Biosciences) pre-coated XF96 plates at 105 cells/well in 175 μL of sodium bicarbonate-free XF Base (Agilent ...
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bioRxiv - Cell Biology 2019Quote: ... low-expressing cells were sorted by FACSAria III (BD Biosciences) and used for experiments ...
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bioRxiv - Cancer Biology 2021Quote: Cells were seeded (2x 105) in T-25 culture flasks (BD-Falcon), allowed to adhere overnight ...
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bioRxiv - Immunology 2022Quote: ... TMRM high or TMRM low (99.5% purity) using a FACSAria (BD).
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bioRxiv - Genomics 2022Quote: ... Specific cell numbers were sorted into low-retention 1.5ml tubes (BD FACSJazz sorter ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed 2x prior to analysis by a Fortessa X-20 (BD). Dead cells were gated out by inclusion of SYTOX Blue or SYTOX AAdvanced dyes (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... cells were additionally fixed and permeabilized in 2x FACS Lysis Solution (BD Biosciences) with 0.08% Tween 20 (National Diagnostics ...
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bioRxiv - Microbiology 2020Quote: ... 500 μL of 2x cold sterile brain-heart-infusion (BHI) broth (BD Difco, 237500) was added to the lysed cells ...
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bioRxiv - Molecular Biology 2019Quote: ... and anti-CD28 (10µg/ml, clone CD28.2, no azide/low endotoxin, BD Bioscience) monoclonal antibodies (stimulated cells) ...
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bioRxiv - Microbiology 2019Quote: ... 5% Skim Milk Protein at 1/2x Tryptic Soy Broth (Becton, Dickinson and company, France) medium and 100 μL of F ...
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bioRxiv - Physiology 2024Quote: ... An equal volume of 2X stain cocktail was made in Brilliant Stain Buffer (BD Biosciences) and added on top ...
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bioRxiv - Cell Biology 2022Quote: ... and low-affinity nerve growth factor receptor (NGFR, also known as p75; BD Pharmingen) for fluorescence-activated cell sorting (FACS) ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... smegmatis (Ms, mc2155) was grown in LB broth (Difco LB Lennox – low salt, BD) under agitation at 37°C overnight ...
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bioRxiv - Immunology 2022Quote: ... Low passage frozen stocks of Mtb were grown to log phase in 7H9 (BD) liquid media supplemented with 10% Middlebrook OADC (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5% w/v Bacto Casamino Acids with low iron and salt content (BD Difco), 1 mM MgSO4 ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed 2x in FACS buffer and fixed using BD Cytofix (BD Biosciences, Cat. 554655) for 15 min at 4°C then washed an additional 2 times and resuspended in FACS buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed 2X with Cell staining buffer and measured with Accuri C6 (BD Biosciences, CA, USA). RNA flow analysis was performed with Amnis® FlowSight® imaging flow cytometer (Luminex Corporation ...
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bioRxiv - Immunology 2021Quote: ... Mitochondria were washed 2X in mitoFLOW buffer and were analyzed on an LSR Fortessa X20 (BD Biosciences). Flow-Jo software was used for post-acquisition analysis MitoTracker green was used to gate on mitochondria.
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bioRxiv - Neuroscience 2019Quote: ... and enhanced green fluorescent protein cDNAs (eGFP; BD Bioscience) as described previously (Raingo et al. ...
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bioRxiv - Immunology 2020Quote: ... Lithium heparin containing vacutainers (green top, BD Biosciences, USA) were used for plasma collection ...
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bioRxiv - Cell Biology 2023Quote: ... with Mouse/Rat Soluble Protein Master Buffer Kit (BD 558266).
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bioRxiv - Cell Biology 2022Quote: ... Cells with high or low expression of tdTomato were selected by FACS (BD Biosciences Aria III).
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bioRxiv - Immunology 2020Quote: ... stained whole blood was fixed and red blood cells lysed with 2X BD FACS Lysing Solution (BD Biosciences). Samples were acquired on a BD Accuri C6 Plus flow cytometer ...
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bioRxiv - Immunology 2023Quote: ... Sytox Green-or PI-positive cells were analyzed by BD FACS Celesta™ Flow Cytometer ...