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Citations for Becton, Dickinson and Company :
401 - 450 of 9014 citations for 3 4 Hydroxy 5 isopropyl 6 oxo 1 6 dihydro pyrimidin 2 ylsulfanyl propionic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... single viable cells were gated and CD45-O1+ and CD45lowSiglec- H+ cells were collected using a FACSAria III and corresponding software (FACSDiva, v.6; BD Biosciences). Calculation of the number of CD45lowSiglecH+ microglia per brain was performed by extrapolating their frequency to the counted total number of isolated cells ...
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bioRxiv - Microbiology 2023Quote: Stm strain SL1344 with plasmid pMW211[C.10E/DsRed] [20] and strain 12023 with plasmid pluxCDABE [6] were recovered from frozen glycerol stock and cultured in Difco Luria-Bertani (LB) Broth (BD Biosciences) containing 100 µg/ml ampicillin (Merck ...
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bioRxiv - Immunology 2023Quote: ... from naïve/PBS-treated or i.n fusion protein immunized mice were isolated from mLN from a pool of 7-8 C57Bl/6 mice and FACS-sorted on a Aria II cell sorter (BD Bioscience). Labeling of cells prior to sorting by flowcytometry (Fusion ...
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bioRxiv - Bioengineering 2023Quote: ... Circulating levels of IL-6 and TNF were determined using a cytokine bead array (BDTM Cytometric Bead Array Mouse IL-6/TNF Flex Set, BD Biosciences). Samples were analyzed in technical triplicates.
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bioRxiv - Cancer Biology 2023Quote: ... a single cell suspension was generated using trypsin and the cells were sorted based on GFP intensity into 6 equally logged bins using BD FACS Aria III (BD Biosciences). In a parallel control condition ...
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bioRxiv - Immunology 2023Quote: Non-pregnant Foxp3eGFP and C57BL/6 females were used to isolate peripheral CD4+ or Treg cells by cell sorting using FACS ARIA (BD Biosciences). Total lymph nodes and spleens were dilacerated ...
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bioRxiv - Genetics 2023Quote: ... we sorted up to 6 million cells for each FlowFISH replicate into 6 bins based on the fluorescence intensity of the target gene using the Influx Cell Sorter (BD Biosciences). To control for differences in staining efficiency of each cell ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then stained for intracellular IL-6 and events were collected using the BD LSR Fortessa cell analyzer (BD Biosciences). Data was analyzed using the FlowJo Software (BD Biosciences).
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bioRxiv - Cancer Biology 2024Quote: Live cells were incubated with 100 nM 6-FAM-dC-puromycin in complete culture medium for 30 min and analyzed by a FACSCalibur flow cytometer (BD Biosciences).
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bioRxiv - Bioengineering 2024Quote: ... or 100kDa conjugates and blood was drawn 6 hours post systemic injection via cardiac puncture and collected in K2EDTA-coated tubes (BD Biosciences). Tubes were centrifuged at 1500 g for 15 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... FITC Mouse Anti-Human CD45 Clone HI30 (RUO) and BV605 Mouse Anti-Human HLA-DR Clone G46-6 (RUO) (BD Biosciences) on Cytek® Northern Lights™ 2000 Flow Cytometer ...
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bioRxiv - Cancer Biology 2024Quote: ... staining mice were injected intravenously through the tail vein with 500 ul of 0.5 mg/mL brefeldin A (BFA) in PBS 6 hours before sacrifice and the staining was performed with the Fixation/Permeabilization Solution kit (BD, 554714).
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bioRxiv - Immunology 2022Quote: Splenocytes from infected mice were incubated for 5 hours at 37°C 5%CO2 in cRPMI prior intra-cytoplasmic detection of active-caspase 3 (BD Bioscience) using BD Fixation/Permeabilization kit (BD Bioscience) ...
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bioRxiv - Molecular Biology 2024Quote: The thymidine analog 5-bromo-2’-deoxyuridine (BrdU; BD Biosciences 550891) was used to assess cell proliferation in vitro ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Mueller-Hinton broth (MH; 17.5 g/L casein acid hydrolysate, 3 g/L beef extract, 1.5 g/L starch; BD Difco), or MH supplemented with 1.5% (w/v ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H: BD construct #8) and each column is a single AD construct (For example column 1 ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining of IL-2 followed the same protocol as that for CTLA-4 but included the addition of 1 μg/mL GolgiPlug (BD Biosciences) to the culture media 12 hours prior to sample preparation ...
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bioRxiv - Microbiology 2020Quote: ... Footpads were digested with 4 mg ml−1 collagenase D for 2 h at 37°C before dissociation through 70 μm cell strainers (BD Biosciences). Homogenates were resuspended in HOMEM supplemented with 20% FCS and serial dilutions (2-fold ...
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bioRxiv - Immunology 2024Quote: ... immunohistochemistry was performed on 4% paraformaldehyde-fixed cryosections of mouse aortic roots using anti-MOMA-2 or anti-CD4 (1:100; BD Biosciences) antibodies ...
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... α-Syn (1:2 000, BD Biosciences ...
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Elevated Hoxb5b expands vagal neural crest pool and blocks enteric neuronal development in zebrafishbioRxiv - Developmental Biology 2021Quote: ... Activated Caspase-3 (1:200, BD Biosciences, 559565). Incubation in primary antibody solutions were conducted overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... active caspase 3 (BD Biosciences, 1:100, rabbit), GFP (abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-LAG-3 (BD, #565616, diluted 1:40), anti-TIGIT (BD ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-TIM-3 (BD, #565567, diluted 1:40), anti-LAG-3 (BD ...
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bioRxiv - Molecular Biology 2024Quote: ... CD34 (BD Biosciences, 560940, 1/5), and retention of marker CD105 positivity and CD45 negativity as well.
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bioRxiv - Immunology 2021Quote: ... both ends of femurs and tibiae were cut with surgical scissors and bone marrow was extracted by flushing 3-5 ml sterile and ice-cold buffer through the bones using a 26G needle (BD Microlance 3 300300). The isolated bone marrow was then filtered through a 40 µM nylon cell strainer (FALCON 352340 ...
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bioRxiv - Cancer Biology 2021Quote: Cells were grown on 6-well plates until 80-90% confluency in presence or absence of 10 μg/ml 9EG7 antibody (BD Biosciences, #553715) for 24 h and scraped off manually with a rubber Corning Cell Lifter (Corning ...
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bioRxiv - Microbiology 2020Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were labeled with murine antibodies against CD3 (BV650) ...
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bioRxiv - Microbiology 2020Quote: ... The sorted GFP− cells were stimulated with 10 ng/mL TNF-α for 6 h and then GFP expression levels were analyzed using BD FACSVerse (BD Biosciences). The level of gag expression after 18-h stimulation with 10 ng/mL TNF-α was analyzed by reverse-transcription (RT)-digital droplet PCR (ddPCR) ...
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bioRxiv - Systems Biology 2022Quote: Approximately 6 mL of whole blood was collected at arrival into K3-EDTA glass blood tubes (BD Vacutainer; Franklin Lakes, NJ, USA) via jugular venipuncture ...
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bioRxiv - Immunology 2021Quote: ... Supernatants from cultured DCs were tested for the production of IL-10, IL-12p70 (eBioscience, USA) and IL-6 (BD Pharmingen, USA) by sandwich ELISA.
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bioRxiv - Immunology 2021Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 4 h of incubation ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected after 6 days of co-culture to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... We used the following 28 antibodies::fluorophore conjugates and clones to enumerate major immune lineages using the Cytek Aurora: HLA-DR::BUV395 (BD; G46-6), CD8::BUV496 (BD ...
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bioRxiv - Immunology 2020Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop™, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
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bioRxiv - Immunology 2023Quote: The plate was incubated 6 hours at 37 °C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 4 hours of culture ...
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bioRxiv - Immunology 2023Quote: ... T cells and RP KD tumor cells were then co-cultured for 6 hours in the presence of GolgiPlug (BD Biosciences 555029) and GolgiStop (BD Biosciences 554724 ...
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bioRxiv - Microbiology 2024Quote: ... The plate was incubated 6 h at 371°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 41h of incubation ...
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bioRxiv - Immunology 2024Quote: ... in a 96-well U-bottom plate in an incubator for 6 hours in the presence of GolgiStop (BD Biosciences, cat#554724) and GolgiPlug (BD Bioscience cat#555029) ...
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bioRxiv - Cancer Biology 2024Quote: High-affinity microplates were sensitized with monoclonal anti-cytokine antibodies (IL-6: 555220; IL-10: 555157; TNF-α: 555212; IL-1β: 557953, OptEIA, BD Biosciences) for 16-18h ...
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bioRxiv - Bioengineering 2020Quote: ... by injecting 2×106 MIA PaCa-2 or PANC-1 cells embedded in Matrigel 1:1 (BD Biosciences) into each flank ...
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bioRxiv - Physiology 2022Quote: ... 0.1 nM retinoic acid) supplemented with 1x penicillin/streptomycin and 2% NuSerum (BD Biosciences, San Jose, CA) as described (39) ...
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bioRxiv - Bioengineering 2021Quote: Six milliliters of blood were collected via venipuncture from five healthy donors and equally divided between two 3 mL tubes of ethylenediaminetetraacetic acid (EDTA) (BD Medical 367856 Vacutainer® ...
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bioRxiv - Neuroscience 2021Quote: ... Expression of the partial recombinant protein of the LymCBP catalytic domain was induced by treatment with 0.32 mM of isopropyl β-D-thiogalactopyranoside (IPTG) in TBG-M9 medium [0.8% Bacto Tryptone (BD) and 0.4% NaCl] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4 μL of the saturated culture were transferred in 60 uL PBS and the samples were stored at 4 °C for 1-2 days before being assayed by flow cytometry (BD FACSCanto II). Data were analyzed in FlowJo ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for 1 hour at 4 °C with 2 µl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Cancer Biology 2021Quote: ... and stained for 1 hour at 4 °C with 2 μl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...