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Citations for Becton, Dickinson and Company :
4351 - 4400 of 5857 citations for Mouse Switch associated protein 70 SWAP70 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... in the last 5 hours of culture a protein transport inhibitor Brefeldin A (1µL/mL) was added (BD Biosciences, USA). Cells were fixed using the BD Cytofix/Cytoperm® kit (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... T cells and tumor cells were co-cultured at a 1:1 effector to target (E:T) ratio for 5 hours in the presence of GolgiStop Protein Transport Inhibitor (BD Biosciences). Surface phenotype of cells was determined by flow cytometry using fluorochrome-conjugated antibodies specific for CD3 (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2019Quote: ... The coding sequence of AGL16 was cloned into pAD-GAL4-2.1 (AD vector) and the putative protein binding sites were cloned into pHIS2 (BD vector). These constructs of pAD and pHIS2 plasmids were introduced into Y187 yeast strain by heat shock ...
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bioRxiv - Biochemistry 2020Quote: ... this threshold is likely a consequence of the fact that the target protein in all six ternary complex crystal structures is a bromodomain (BD), of either Brd4 ...
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bioRxiv - Immunology 2020Quote: ... 1–2 × 106 cells were incubated with 1 μM of SARS-CoV-2 minimal epitope or pool of up to ten epitopes (1 μM/peptide) for 9 h at 37°C in the presence of protein transport inhibitor (GolgiPlug; BD Biosciences ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Proteins were detected using anti-HA (Covance cat# MMS-101P, 1:1000) and anti-Actin (BD cat# 612657, 1:1000) antibodies ...
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bioRxiv - Immunology 2020Quote: ... The levels of IFNg protein expression was determined by intracellular staining after exposure to 4 hours of GolgiStop and GolgiPlug (BD).
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bioRxiv - Immunology 2020Quote: ... IFN-γ and IL-4 protein in culture supernatants were quantified using the Cytokine Bead Array according to the manufacturer’s instructions (BD Biosciences)
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bioRxiv - Immunology 2021Quote: ... FNA samples were stained in P2 for 30 minutes on ice with biotinylated and Alexa Fluor 647 conjugated recombinant soluble Spike proteins as well as PD-1 BB515 (EH12.1, BD Horizon). Cells were then washed twice with P2 and stained with IgG BV480 (goat polyclonal ...
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bioRxiv - Biochemistry 2024Quote: ... AtHSP90-3 and ASDURF were transferred to the pGADT7-GW and pGBKT7-GW destination vectors using Gateway LR Clonase II enzyme mix to produce fusion proteins of the Gal4-activation domain (AD) and GAL4 DNA-binding domain (BD), respectively ...
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bioRxiv - Immunology 2024Quote: ... cells were treated for 5 hours with a protein transport inhibitor containing brefeldin A (BD Biosciences, Franklin Lakes, NJ, USA), washed and fixed for 10 minutes at 4°C with fixation/permeabilization solution (BD Cytofix/Cytoperm kit ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes containing the transferred proteins were rinsed and blocked in 0.1% Tween-20 in Tris-buffered saline (TBST) containing 5% nonfat milk (BD Biosciences) at RT for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: Cell cycle analysis was performed using the BrdU Flow Kit according to the manufacturer protocol (BD, FITC BrdU Flow Kit; Cat. No. 559619), with cells pulsed with BrdU for 1 hour at 37°C ...
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bioRxiv - Bioengineering 2024Quote: pEU-E01 cell-free wheat germ expression vector containing ampicillin resistance and cell-free wheat germ expression kit (Cat# CFS-CPLE-BD Proteoliposome BD Kit) were obtained from CellFree Sciences Co. ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used are as follows and were used according to manufacturer’s protocol: PE-Cy7 Mouse Anti-Human CD3 (Cat 563423; BD Pharmingen), PE Mouse Anti-Human CD4 (Cat555347 ...
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bioRxiv - Immunology 2020Quote: ... 2mM EDTA and 0.05% NaN3): PerCP-Cy™5.5 mouse anti-human CD19 (1:40 dilution, Clone HIB19, BD Pharmingen) (for PBMC samples only) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibody in incubation buffer overnight at 4°C with mouse anti-CtBP2 antibody (1:50, BD Transduction ...
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bioRxiv - Immunology 2022Quote: ... cells were first stained for surface markers and then fixed and permeabilized using Intracellular Fixation and Permeabilization Buffer Set (eBioscience) before incubation with fluorochrome-conjugated mAbs against mouse IFNγ (clone XMG1.2, BD Bioscience). Flow cytometry was performed on LSRFortessa (BD Biosciences ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated in blocking buffer (0.1% Triton X-100 plus 10% normal goat serum in DPBS) for 20 minutes followed by overnight incubation in primary antibodies at 4°C (mouse anti-GM-130, BD Transduction laboratories #610822 1:250 ...
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bioRxiv - Cell Biology 2019Quote: Anti-Cav-1 rabbit polyclonal (cat # 610059) and anti-phos-Y14-Cav-1 mouse monoclonal (cat # 611338) antibodies were from BD Transduction (San Jose ...
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bioRxiv - Cancer Biology 2019Quote: ... xenografts and mouse brain tissue were embedded in 3-4% (w/v) Difco Noble agar (BD Biosciences, San Jose, California) in distilled water ...
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bioRxiv - Bioengineering 2019Quote: ... Sections were co-incubated overnight with rat monoclonal anti-mouse MAdCAM-1 (1:1000; clone MECA-367 from BD Biosciences) and goat anti-collagen-type IV (1:1500 ...
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bioRxiv - Immunology 2019Quote: ... cells from different stimulation conditions were permeabilized and fixed according to the manufacturer’s instructions with the FOXP3 permeabilization kit (Foxp3/Transcription Factor Staining Buffer Set ebioscience) and then labelled with an anti-Ki-67 antibody (clone B56, mouse IgG1; BD) and DAPI was added before flow cytometry analysis.
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bioRxiv - Immunology 2019Quote: ... Samples were then washed twice in the FACS buffer and incubated with anti-mouse IgG1-PE (BD Biosciences, ref.#550083) and anti-rabbit IgG-AlexaFluor647 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... In experiments testing double recombinants SARS-CoV2 vectors IL-2 antibody was not included and PE-CF594 anti-mouse IL-4 (clone 11B11, 562450, BD) and BV421 rat anti mouse IL-10 (clone JES5-16E3 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated for 1 h at RT with primary antibody used as follows: mouse anti-EEA1 (BD Biosciences: 610457) 1 µg/mL ...
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bioRxiv - Immunology 2019Quote: ... Cell surface markers were immunolabeled with anti-Gr-1 (PE Rat Anti-Mouse Ly6G and Ly6C, BD Pharmingen, Franklin, NJ), F4/80 (Anti-F4/80 PE ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed 3 times with PBS-T and 50μl of either biotinylated rat anti-mouse IgG1 (1:500, BD Bioscience), rat anti-mouse IgG2a (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumor xenografts were established in 6-week-old NOD SCID mice via subcutaneous injection into the dorsal region of each mouse with 1 x 107 cells/0.15 ml Hank’s balanced salt solution containing Matrigel® (BD Biosciences). Experiments were performed at five mice in each group (vector control ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used for immunostaning the cells were : CD3+ (Mouse anti-pig CD3ε Alexa Fluor 647; BD Pharmingen, catalog no: 561476), CD4+ (Primary antibody ...
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bioRxiv - Immunology 2021Quote: ... and then stained with the fluorochrome-labeled anti-mouse CD3/CD4/CD8/CD44/CD62L mAbs (all purchased from Biolegend except CD44 from BD). Cells were then treated with Cytofix/Cytoperm (BD ...
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bioRxiv - Neuroscience 2020Quote: ... to improve staining quality and with 2,5 mL per sample of each of the following anti-mouse mAbs: Ly6G BB515 (Clone: 1A8; BD, USA), CD45 BV786 (Clone ...
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bioRxiv - Neuroscience 2020Quote: ... mouse primary at 1/100, Dako; CldU: rat primary at 1/1,000, Accurate Chemical; IdU: mouse primary at 1/1,000, BD Biosciences) and with a rabbit anti GFP (Millipore ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used in this study: mouse monoclonal antibodies against Rab6 (Schiedel et al., 1995) and Ku80 (BD Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were activated by seeding in 96-well plates pre-coated with anti-mouse CD3e (1 μg/ml working concentration, Clone: 145-2C11, BD) and anti-mouse CD28 (2 μg/ml working concentration ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured in T cell media supplemented with soluble anti-mouse CD28 (2 μg/ml, Clone: 37.51, BD Pharmingen) and 30 U/ml rhIL-2 (Roche ...
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bioRxiv - Bioengineering 2021Quote: ... at 2×106 cells/ml in wells coated with anti-mouse CD3e (1 μg/ml, Clone: 145-2C11, BD Pharmingen).
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibodies diluted in PBS containing 2% NHS overnight (1:500 rabbit anti-Iba1, Abcam, ab178846; 1:200 mouse anti-Map2, BD Pharmagen ...
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bioRxiv - Cell Biology 2021Quote: ... 1:500 for immunofluorescence or IF) and mouse anti-Golgin245 mAb (#611280, 1:200 for IF) were purchased from BD Bioscience ...
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bioRxiv - Neuroscience 2019Quote: ... Drug solutions were IP injected in 0.1 ml solution/10 g of mouse using a fine needle insulin syringe (BD 324911, BD Bioscience). On average ...
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bioRxiv - Bioengineering 2021Quote: ... live/dead stain in PBS for 15 min on ice before washing and re-suspending in 2x human/mouse Fc block in stain buffer (BD) followed by surface-staining with monomeric GFP and anti-human CD45 AF700 (Biolegend clone HI30) ...
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bioRxiv - Systems Biology 2021Quote: ... Similarly detached hCMEC/D3 cells were incubated with APC-conjugated mouse monoclonal anti-BCRP (1:100, BD Biosciences, Oxford, UK), or PE-conjugated mouse monoclonal anti-MDR1A (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C with primary antibodies (Guinea-pig anti-VGAT, Synaptic Systems 131004, 1μg/mL, 1:200 and mouse anti-mGluR1, BD Pharmingen,0.5mg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Immunocytochemical staining using a rat monoclonal anti-mouse CD31 antibody (1:100; clone MEC 13.3; BD Pharmingen, San Jose, CA) was used to detect endothelial cells in the mouse heart ...
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bioRxiv - Microbiology 2021Quote: ... samples were re-suspended in 25 μl of anti-mouse CD16/CD32-Fc/Block (BD Bioscience, San Jose, CA, USA), incubated 15 minutes on ice and then stained at RT in the dark for 20 minutes with the following mixture of fluorescent-labeled antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-EEA1 mAb (#610456; 1:500 for IF) and mouse anti-GM130 mAb (#610823; 1:500 for IF) were from BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 300µL of PBS + 1% v/v FBS and 150µL were added to 2µL of Mouse BD Fc Block (BD Biosciences 553142). Homogenates in Fc block were incubated for 15 minutes at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... the sections were incubated with HypoxyprobeTM rabbit anti-pimonidazole antibody (1:250; hpi) for pimonidazole staining or rat anti-mouse CD31 antibody (1:250; BD) for CD31 staining overnight at 4℃ ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse Anti-human KDR (KDR-PE, FAB357P, R&D) and Mouse Anti-Human NRP-1 (NRP-1-PE, Material Number: 565951, BD) antibodies were used at a ratio of 1:20 ...