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Citations for Becton, Dickinson and Company :
4151 - 4200 of 4267 citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Pelleted cells were incubated with anti-GFP-AF647 antibody (1:150, Alexa Fluor 647 Mouse Anti-GFP Clone 1A12-6-18, BD Biosciences 565197) or rabbit anti-ITGA5 (1:100 ...
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bioRxiv - Cell Biology 2022Quote: The following antibodies were obtained commercially and used at the indicated dilutions for immunofluorescence: mouse monoclonal TOM20 (BD Biosciences #612278, 1:1000), TOMM20 (Abcam #ab186734 ...
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bioRxiv - Immunology 2022Quote: ... Transduction efficacies were determined via fluorescence activated cell sorting (FACS) staining with TCRmu antibody (anti-mouse TCR-β-chain, FITC, BD Biosciences) against the murine-β-chain of engineered TCR-constructs in comparison to non-transduced T cell populations.
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bioRxiv - Cell Biology 2022Quote: ... the cells were blocked with 2% BSA in 0.1% tween TBS (TBST) for 1h and immunostained with mouse anti-paxillin IgG1 antibody (BD Transduction Laboratories 610052) (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used in this study: anti-total α-syn mouse monoclonal Syn-1 (1:10,000 dilution; BD Biosciences #610786), anti-PSyn rabbit monoclonal EP1536Y (1:10,000 dilution ...
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bioRxiv - Microbiology 2024Quote: Lung cytokines were quantified from the supernatant of thawed organ homogenates (stored at - 80°C) from each experimental timepoint using the BD Mouse Th1/Th2/Th17 Cytometric Bead Array (CBA) Kit (BD Biosciences, 560485). Briefly ...
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bioRxiv - Immunology 2024Quote: ... MASP-1/-3/MAP-1 mab 8B3 or F(ab’)2 fragments generated from an irrelevant mouse IgG1 mAb clone MOPC-31C (BD Biosciences, 557273) were coated on maxisorp plates at 4 °C ON and afterwards washed and blocked for 1 hr in TBS/T ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with FACS staining buffer (PBS supplemented with 2% fetal bovine serum and 1 mM EDTA) and incubated with Fc receptor blocker purified rat anti-mouse anti-CD16/CD32 (BD Biosciences, 553142) for 20 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cells were spun at 500xG and resuspended in Flt3L DC Buffer (T-cell buffer + 10% B16-Flt3L conditioned RP10G medium + 1000U/mL Mouse GM-CSF [BD Biosciences, #554586]). Dendritic cells were plated in a total volume of 75 μL and rested overnight ...
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bioRxiv - Cell Biology 2023Quote: ... VE-cadherin was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD144 (BD Pharmingen™), 2 min prior to imaging ...
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bioRxiv - Biochemistry 2023Quote: ... cells were processed as for CDC45 but without the methanol fixation step and with a 1:1000 dilution of mouse monoclonal anti-MCM2 (BD Biosciences, 610701) for 14 hours at 4°C ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 10 ng/mL anti-CD3 mAb at 4°C overnight (Purified NA/LE mouse anti-human CD3, BD Cat# 557052). Three-fold serial dilutions of either IL-7 or a test compound were added to the cells and incubated for 4 days at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow from each mouse was then combined and subjected to red blood cell lysis in 1X BD PharmLyse (BD Biosciences, 555899). Cells were then washed with PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... and stained with the following monoclonal anti-mouse antibodies as described in results: GR1 FITC (clone RB6-8C5, BD Biosciences, RRID: AB_394643), F4/80 FITC (clone BM8 ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... Permeabilization was followed by blocking cells using 5% BSA in HBSS+ for 30 min and incubated with the primary antibody solution - Mouse anti-human CD144 (1:150; 555661, BD Biosciences, USA) for overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Blots were probed for 1h at room temperature (RT) with a mouse anti-GFP antibody (Living Colors -JL-8, BD Biosciences Clontech) in TBS-T buffer (20 mM Tris ...
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bioRxiv - Genomics 2023Quote: ... or with the appropriate class control antibodies: PE Mouse anti-IgG1 κ R-PE Clone MOPC-21 (BD Biosciences; Catalog no. 559320) and Alexa Fluor® 647 Mouse anti IgG1 κ Isotype Clone MOPC-21 (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-GluNR2B (1:1,000 for #AGC-003, Alomone lab) or mouse anti-GluN2B (1:500 for #610416, BD Transduction Lab, NJ), rabbit anti-NR2C (1:1,000 for #AGC-018 ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were then incubated for 1 h with primary antibodies directed against the cis-Golgi marker GM130 (mouse anti-GM130, BD bioscience, 610822) and/or trans-Golgi marker TGN46 (sheep anti-TGN46 ...
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bioRxiv - Immunology 2024Quote: ... followed by two washes with the permeabilization buffer before incubation with 4 μg/ml Alexa Fluor® 647 Mouse anti-Phospho-NF-κB p65 (pS529) (BD Biosciences) for 45 min.
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bioRxiv - Immunology 2024Quote: ... Surface staining was carried out for 30 minutes at 4°C with the following fluorochrome-labeled anti-mouse Abs: APC/Cy7-CD45 (BD Bioscience, #557659), PE/Cy5-B220 (eBioscience ...
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bioRxiv - Cell Biology 2024Quote: ... They were stained for 2 hours at room temp with the following primary antibodies: mouse anti-GM130 (1:250, BD Bioscience, BDB610822) and rabbit anti-TGN46 (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with a fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD31 antibody (1:50; BD Pharmingen, San Diego, CA, USA) for 30 min at room temperature followed by 3 washes with phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: Eosinophils of 6-12 week-old Il5-tg females and males were positivelyenriched using PE anti-mouse Siglec F antibody (562068 BD Biosciences; E50-2440) and anti-PE microbeads (130-042-401 Miltenyi Biotech) ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated for 60 minutes in 1% BSA and 5% goat serum in PBS and then incubated overnight with anti-p21 antibody (BD Pharmingen, mouse, #556431) and anti-p16 antibody (abbiotec ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then left at 4°C overnight in a blocking solution containing the primary antibody: mouse E-Cadherin (BD Biosciences, 1:1000) and mouse N-Cadherin (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... the cryosections were incubated for 1 h in the blocking solution (PBS containing 5% goat serum and 5 μg/mL mouse BD Fc Block™) and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with 2M HCl for 30 minutes and stained with mouse monoclonal anti-BrdU antibody (1:50) (BD Biosciences, San Jose, CA, USA) and Alexa fluor-488 conjugated secondary antibody (1:200 ...
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bioRxiv - Neuroscience 2020Quote: Western analyses were run as described[33] with the following changes: Purified mouse anti-ASyn antibody clone 42 (BD Biosciences, San Jose, CA) was diluted at 1/1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were incubated overnight at 4 °C with primary mouse monoclonal anti-alpha synuclein antibody (Cat no. 610787; BD Biosciences; 1:200) and for 2 hr with secondary goat anti-mouse Alexa Fluor Plus 488 antibody (Cat no ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were thawed and sandwich ELISA for human or mouse IFN-γ levels was performed using the BD Human IFN-gamma ELISA Set (BD Biosciences, 555142) or BD Mouse IFN-gamma ELISA Set (BD Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... WBM cells were blocked with Fc Blocker and stained with biotinylated-Lineage antibody cocktail according to the Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit by BD (Catalog No. 560492). The stained cells were labelled with streptavidin magnetic particles and placed into BD IMagnet (BD ...
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bioRxiv - Genomics 2022Quote: ... harvested bone marrow cells were labeled using a two-step sequential antibody labeling procedure using the following primary conjugated antibodies: 1:200 dilution of Anti-Mouse CD5 FITC antibody (BD Biosciences, Cat#: 553020), 1:100 dilution of Anti-Mouse B220 FITC antibody (BD Biosciences ...
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bioRxiv - Genomics 2022Quote: ... Cells were labeled with the following antibodies: 1:400 dilution of Anti-mouse CD45 antibody conjugated to APC-Cy7 (BD Biosciences, Cat#: 557659), 1:200 dilution of Anti-mouse TER-119 antibody conjugated to APC-Cy7 (BD Biosciences ...
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bioRxiv - Microbiology 2019Quote: ... Cells were transferred to a 96 well U-bottom plate and Fc receptors on cells were blocked with Rat anti-mouse Fc-Block CD16/CD32-Clone 2.4G2 (BD Pharmigen, San Jose, CA) on ice for 15 minutes ...
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bioRxiv - Immunology 2019Quote: ... Cytokine concentration was quantified in the supernatants by cytometric bead array (CBA) according to the manufacturer’s instructions from the Mouse/Rat Soluble Protein Flex Set System kit (BD Biosciences Pharmingen, Oxford, UK). CBA data was analysed on a MACSQuant analyser with MACSQuantify™ Software and the CBA analysis software package (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Antibody concentrations were as follows: alveolar macrophage membranes were stained using CD206 PE-CF594 (Mouse Anti-Human CD206, BD Biosciences; catalogue number: 564063) at a concentration of 4µL in a 50μL final staining volume per section ...
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bioRxiv - Cell Biology 2019Quote: The following antibodies were used for fluorescence microscopy: mouse IgG2B anti-human DC-SIGN at 2 μg/ml (DCN46, BD Biosciences, Breda, Netherlands), mouse IgG1 anti-human AZN-D1 at 2 μg/ml (Geijtenbeek et al. ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with PerCP/Cy5.5 or BV421-conjugated anti–mouse CD4 rat monoclonal antibody (RM4-5; BD Biosciences, Franklin Lakes, NJ, USA). The cells were then fixed and permeabilized with Cytofix/Cytoperm reagent (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... IdU and CldU were then detected with the following primary antibodies in blocking solution for 1 hour at room temperature: mouse anti-BrdU (B44) (1:25) (BD Biosciences cat#347580) for IdU and rat anti-BrdU [Bu1/75 (ICR1)] (1:25 ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody incubated for one hour at RT in 5% milk TBS-T at 1:1,000 (HRP-Anti-Mouse, BD Pharmigen, Goat Polycloncal, 554002). Between steps 3 × 5 minute washes were performed in TBST ...
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bioRxiv - Immunology 2020Quote: ... Supernatant was collected and secreted TNFα protein levels were measured using a BD OptEIA Mouse TNFα Enzyme-Linked Immunosorbent Assay (ELISA) kit (BD Biosciences, Mississauga, ON). Triplicates wells were used for each stimulation condition ...
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bioRxiv - Immunology 2021Quote: NK cells and cancer cells were cocultured at 10:1 ratio and supplemented with 20ul of PE mouse anti-human CD107a antibody (BD Pharmingen™, #555801) in a total volume of 220ul in a 96 well plate at 37C incubator for 90 minutes ...
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bioRxiv - Immunology 2020Quote: ... 7 days post-vaccination) and measured Cytokines using the BD CBA Mouse Th1/Th2/Th17 Cytokine Kit (BD Bioscience, San Jose, CA, USA). Sera samples were processed as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... fluorescein isothiocyanate (FITC)- mouse anti-human Annexin-V (biolegend; Cat# 640945) and APC-anti-human anti-CD42b (GPIb) monoclonal antibody (BD Pharmingen; Cat# 551061). The calcein AM blue was used to identify live platelet sized events ...
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bioRxiv - Immunology 2022Quote: ... the individual antibodies used for staining were mixed with one drop of negative control beads and anti-mouse IgG beads (BD Biosciences, CA, USA). For the dead cell stain ...
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bioRxiv - Immunology 2022Quote: Surface staining: Single cell suspensions were incubated with 5% normal rat serum and rat anti-mouse CD16/CD32 antibody (BD Biosciences close 2.4G2) then stained with fluorophore-conjugated antibodies ...
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bioRxiv - Microbiology 2022Quote: ... was used as primary antibody for samples and mouse anti-clathrin heavy chain clone 23 (1:2,000; BD Biosciences, Franklin Lakes, NJ, USA) for the detection of clathrin heavy chain as a loading control ...
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bioRxiv - Genomics 2024Quote: ... 2.5 x 105 cells were stained for 75 minutes at room temperature with PE Mouse anti-PDX1 Clone-658A5 (BD Biosciences; 1:10) and Alexa Fluor® 647 Mouse anti-NKX6.1 Clone R11-560 (BD Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... and samples were incubated in the 9-well plates with 100 μL per plate overnight at 4°C: CtBP2 mouse IgG1 (BD Biosciences 612044; RRID:AB_399431), GluA2 mouse IgG2a (Millipore MAB397 ...