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Citations for Becton, Dickinson and Company :
351 - 400 of 4386 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... France) were differentiated on filter membranes (Individual Cell Culture Inserts with 0.4 μm pore size, BD Biosciences, San Jose, CA, USA) [22 ...
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bioRxiv - Cancer Biology 2021Quote: ... transfected/Dox treated stable cells (5×105 cells) in serum-free media were seeded in the upper chamber of an insert (8 mm pore size; BD Bioscience) coated with Fibronectin (BD Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was again discarded and pellets suspended in 300 µL of 0.02 µm-pore filtered before being transferred to BD Trucount™ Tubes (BD Biosciences) and proceeded to Flow Cytometry analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... 2.5×10^4 cells resuspended in serum free media was then added to the top 8 µm pore matrigel coated (invasion) or non-coated (migration) trans-well insert (BD Biosciences). After 20 hours ...
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bioRxiv - Cell Biology 2022Quote: ... and plated on the upper chamber of the 24-well format HTS FluoBlokTM Multiwell Insert System (3 μm pore size; BD Falcon) in RPMI without phenol red (Life Technologies ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: Individual spleens were removed and mechanically dissociated using a syringe plunger above 70 μm-pore size Falcon cell strainer (BD Biosciences). Red blood cells were lysed using ACK lysing buffer (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... the cell invasion assay was performed using Transwells (8 μm pore size, millipore) with inserts coated with Matrigel (50 mg/ mL, BD Biosciences). 786-O and ACHN cells (1.0 × 105 cells/well ...
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bioRxiv - Cancer Biology 2024Quote: ... A 30min permeabilization step using True-Nuclear™ Transcription Factor Buffer Set (424401) was performed before staining for SOX2 (561610, BD Biosciences) during 60min ...
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bioRxiv - Immunology 2020Quote: ... CLTX-CAR T cells were incubated with GBM cells (E:T=1:1) for 5 h in the presence of CD107a antibody and GolgistopTM protein transport inhibitor (BD Biosciences). To test for CAR T cell killing potency ...
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bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... cells were washed again and re-suspended in permeabilization buffer with the following fluorescently-tagged antibodies targeting intracellular T-lymphocyte proteins for 30 min: IL-4 BV 421 (BD Biosciences clone 11B11 ...
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bioRxiv - Cancer Biology 2020Quote: ... and knockout success was examined by Sanger sequencing and Western blot to confirm protein loss (anti-CDH1 antibody, BD #610182). 8 clones with the least E-cadherin protein expression by immunoblot were then pooled in equal ratio and named T47D CDH1 KO ...
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bioRxiv - Genomics 2020Quote: ... Primary antibodies (anti-Cas9 7A9-3A3, Cell Signaling Technology #14697S, anti-γ-tubulin Sigma#T5326, anti-Human Retinoblastoma protein BD Pharmigen #554136 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were stained with antibodies specific for intracellular proteins (Supplemental Table 1) (1:100,v/v) in Perm/Wash buffer (BD) supplemented to contain 2% rat serum at RT for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... Sections were stained with biotin labeled anti mouse IgD (eBioscience) and PE labeled anti mouse BCL6 (BD Biosciences) antibodies ...
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bioRxiv - Immunology 2020Quote: ... CD103 (APC, B-Ly7, eBioscience, or biotin, 2G5, Beckman Coulter, or Ber-ACT8, Brilliant Violet 711, BUV395, BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse NK1.1 (clone: PK136, Biotin, eBioscience, cat# 13-5941-85; clone: PK136, PE, BD Pharmingen, cat# 553165), anti-mouse CD19 (clone ...
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bioRxiv - Cancer Biology 2024Quote: ... HSPCs were isolated utilizing a negative selection methodology via the BD Biotin Mouse Lineage Panel kit (BD #559971). For the erythroid cells ...
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bioRxiv - Microbiology 2020Quote: ... the cell suspension was stained with a LIVE/DEAD fluorescent dye (7-AAD; BD Biosciences) for 10 min ...
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bioRxiv - Immunology 2020Quote: ... Proliferation was analyzed using CFSE and CellTrace Violet dye dilution on the LSRFortessa (BD Biosciences) and FlowJo.
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Asymmetric Binomial Statistics Explains Organelle Partitioning Variance in Cancer Cell ProliferationbioRxiv - Systems Biology 2021Quote: Jurkat cells labeled with dyes were sorted using a FACSAriaIII (Becton Dickinson, BD Biosciences, USA) equipped with Near UV 375nm ...
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bioRxiv - Immunology 2021Quote: ... PBMC were thawed and labeled using Violet Proliferation Dye-450 (BD Biosciences, San Jose, CA) and combined with DCs at a 1:4 ratio ...
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded from the analysis using fixable viability dye (FVD) BV421 (BD Biosciences). To assess the expression of cytokines ...
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bioRxiv - Cancer Biology 2024Quote: ... and hematopoietic cells stained with Fixable viability dye eFluor 455 (BD Biosciences 65-0868-14), murine Fc block (Bio X Cell BE0307) ...
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bioRxiv - Cancer Biology 2020Quote: ... 5×104 cells were added to the top chamber of 24-well transwells (8 μm pore size membrane; BD Bioscience, Bedford, MA), and assay medium was added to the bottom chambers and incubated for 24 hours (10% FBS) ...
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bioRxiv - Cancer Biology 2022Quote: Trans-well assay was done in 24-well Boyden chambers with 8-μm pore size polycarbonate membranes (BD Biosciences, San Diego, USA). ST1 cells (5 × 104 ...
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bioRxiv - Cancer Biology 2020Quote: ... 5×104 were added to the top chamber of 24-well Boyden chambers (8 μm pore size membrane; BD Bioscience, Bedford, MA), and medium was added to the bottom chambers and incubated for 24 hours (DMEM 10% FBS) ...
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bioRxiv - Cell Biology 2020Quote: ... 1.5 × 105 cells were plated in the top wells of Growth Factor Reduced Matrigel-coated invasion chambers (8 μm pore size, BD Bio Coat). Media containing 5% was added to the lower chamber ...
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bioRxiv - Cancer Biology 2020Quote: ... and cell invasion was investigated by using 24-well transwell inserts that had been precoated with matrigel with 8 μm pores (cat No. 354480, BD Biosciences, USA). A total of 1×105 cells from the indicated treatments were cultured in serum-free medium in the upper chamber ...
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. Surface expression level of S proteins (Extended Data Fig. 6a) was measured using FACS Canto II (BD Biosciences) and the data were analysed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interest were visualized after incubation with primary antibodies (α-synuclein 1:1000 BD Biosciences (San Jose, CA, USA) #610787 ...
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bioRxiv - Immunology 2022Quote: PBS-washed cells were PFA-fixed and immunostained for individual surface protein expression using the following antibodies: Anti-CD3-FITC (#561807; BD Biosciences), anti-CD4-APC (#555349 ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Tissues were incubated at 4°C overnight with the following primary antibodies: monoclonal mouse anti-carboxyl-terminal binding protein 2 (CtBP2) IgG1 at 1:200 (612044; BD Biosciences) counterstained with goat anti-Mouse IgG1 conjugated with Alexa Fluor 568 (#A-21124) ...
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bioRxiv - Immunology 2020Quote: PBMCs or purified T cells were stained for 30 minutes on ice with the antibodies specific for extracellular proteins in Brilliant Stain Buffer (BD, 563794). Following incubation ...
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bioRxiv - Cell Biology 2020Quote: ... the cell surface of freshly-prepared epidermal cells was labeled with the following antihuman protein antibodies (clone, manufacturer): FITC–conjugated CD1a (HI149) and PE–conjugated HLA-DR (G46-6, both BD Pharmingen), PE–CF594–conjugated CD3 (UCHT1 ...
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. The surface expression level of S proteins (Fig. 3d) was measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. The surface expression level of S proteins (Fig. 3d) was measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... was used as a secondary antibody. The surface expression levels of S proteins (Fig. 4A) were measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (FlowJo LLC) ...
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bioRxiv - Neuroscience 2022Quote: ... that was blocked in 5% non-fat milk for 1 h at room temperature and incubated overnight with primary antibodies against the following proteins: α-synuclein (1:1000, BD), NCK2 (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were stained with antibodies specific for surface proteins (Supplemental Table 1) (1:100, v/v) in Brilliant Stain buffer (BD, 566349) at 4°C for 30 minutes and washed with FACS buffer ...
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bioRxiv - Immunology 2024Quote: ... 10 μg/ml of ESAT-6 and CFP-10 full proteins (BEI Resources) + anti-human CD28/CD49d antibodies at 1 μg/ml (BD Bioscience). Samples were incubated at 37°C and 5 μg/ml of Brefeldin A (Sigma-Aldrich ...
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bioRxiv - Pathology 2024Quote: ... and then incubated overnight at 4 °C with primary antibodies directed against amino acids 15–123 of the α-Synuclein protein (1:1,000 dilution, ref: 610786, BD Biosciences) diluted in the blocking buffer ...
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bioRxiv - Immunology 2021Quote: ... erythrocyte-depleted BM cells were stained first for lineage markers with biotin-labeled mouse lineage panel (BD Biosciences, Pharmingen) containing anti-CD3e (CD3Ɛ chain) ...
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bioRxiv - Cancer Biology 2020Quote: ... 6.6×105 starved MDAMB231 cells expressing the shRNA library (or control cell line) were plated onto 6-well transwell chambers (8 μm pore size membrane; BD Bioscience, Bedford, MA) in assay medium ...
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bioRxiv - Cancer Biology 2020Quote: ... 6.6×105 starved MDA-MB-231 cells expressing the shRNA library (or control cell line) were plated onto 6-well Boyden chambers (8 μm pore size membrane; BD Bioscience, Bedford, MA) in assay medium ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was seeded into the upper chamber of SPLInsert with Polyethylene terephthalate (PET) membrane (pore size: 8.0 μm) (SPL Lifesciences, Korea) pre-coated with matrigel (BD Biosciences, San Jose, CA). The bottom well of 24-well plate was filled with cell medium containing 10% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... and allowed to gel over a 1-mL layer of acellular collagen in six-well culture inserts with 3-μm-pore polycarbonate filters (BD Biosciences NJ, USA). Gels were allowed to contract for 4–5 days before seeding with 3×105 HaCaT keratinocytes in serum and mitogen-supplemented DMEM/F12 raft medium ...
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bioRxiv - Cell Biology 2021Quote: ... The viability dyes DAPI and 7-AAD were used where appropriate (BD and Thermo Fisher Scientific). CellTrace Far Red (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... PerCP and cyanine dye Cy5.5 (PerCP Cy5.5)-conjugated anti-Fcγ-RII/III (clone 2.4G2) (BD Biosciences). FACS sequential discrimination on a Lineage negative gated population was used to identified LK myeloid progenitor subpopulations ...