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Citations for Becton, Dickinson and Company :
351 - 389 of 389 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: Cells were plated at 5-15K the day prior to transfection in a 96-well plate coated with poly-D-lysine (BD Biocoat). HEK293FT and HepG2 cells were transfected with Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... were stained with the specified antibodies on ice for 30 min in D-PBS supplemented with 2% FBS in the dark and analyzed using a Fortessa or Symphony A3 (BD Biosciences). Data was analyzed using FlowJo10 (Tree Star Inc. ...
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bioRxiv - Genetics 2023Quote: ... and CD43-B cells were cultured at a density of 5×105 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). Cultured cells were maintained daily at a density of 0.5-1×106 cells per ml ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were then dissociated and washed two times with D-PBS before fixing and permeabilisation for 20 minutes (554714 Cytofix/Cytopearm, BD Biosciences). Astrocytes were washed a further two times in wash buffer before incubation with IL-6-PE (12-7069-82 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then resuspended in FACS buffer (1% BSA in D-PBS) and kept at 4°C in dark until analyzed with a customized BD LSR Fortessa flow cytometer (BD Biosciences). Compensation was performed using ArC and UltraComp compensation beads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... overnight cultures of each strain were grown in BHI media and spot plated on agar plates made from tryptic soy broth without added dextrose (TSB-D, BD Difco) supplemented with 3% (wt/vol ...
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bioRxiv - Immunology 2022Quote: ... all cells were stained for L/D staining and acquisition was performed on the Fortessa LSR flow cytometer (BD B ioscience) and analyzed with Kaluza (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and washed with ice-cold PBS twice and resuspended in annexin V binding buffer (#556454 BS bioscience) and incubated at RT in the dark with 7-amino-actinomycin D (7-AAD, #559925, BD bioscience) and annexin-V for 15 min ...
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bioRxiv - Genetics 2024Quote: ... The resultant nuclei suspension was FACS sorted using 7-Aminoactinomycin D (7-AAD) dye as a singlet discriminator on FACS Jazz cell sorter (BD Biosciences). 600,000 sorted events were collected in 10% BSA in 1X PBS solution supplemented with RNase inhibitor ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Organoids were cultured for 14 d for counts to monitor formation and were harvested from Matrigel using cell recovery solution (BD Biosciences). For human epithelial cell cultures ...
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bioRxiv - Molecular Biology 2021Quote: ... It was retrieved from frozen glycerol stocks and cultivated for 24 h at 28 °C on tryptic soy agar (TSA, Becton, Dickinson and Company, USA), followed by culturing in 100 mL of tryptic soy broth (TSB ...
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bioRxiv - Immunology 2021Quote: ... Assay plates were incubated 24-48 h at 37⍰°C in a 5% CO2 incubator and developed using BD ELISpot AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISpot reader and ImmunoSpot®software v6 (Cellular Technology ...
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bioRxiv - Physiology 2021Quote: ... A 3% BSA-0.2% Triton®X-100 solution was used for permeabilization and blocking followed by a 1 h RT incubation with PBS–1% BSA containing mouse monoclonal DRP1 antibody (611113, BD Transduction Laboratories, 1:50) followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121 ...
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bioRxiv - Immunology 2021Quote: ... The stimulation was performed by incubation for 6 h at 37°C and 5% CO2 in the presence of Protein Transport Inhibitor Cocktail (brefeldin A) (BD Biosciences, San Jose, CA) at a final concentration of 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: Blood extractions were performed in the morning (8:00-10:00 a.m.) after 8-12 h of fasting and extracted into 10mL BD vacutainer® tubes with K2-EDTA anticoagulant (BD, Franklin Lakes, NJ, USA). Blood samples were immediately processed to obtain plasma and PBMCs based on our previous studies (Orio et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cells were incubated for 4 h and washed twice with PBS for analysis of fluorescein positive cells via flow cytometer (BD FACSCantoTM II, BD Biosciences). To further investigate the internalization mechanism of PoLixNano ...
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bioRxiv - Biochemistry 2022Quote: ... 10 μL pre-cleared cell lysate was combined with 200 μL assay reagent containing 1 mM D-luciferin (BD Biosciences, product #556878), 3 mM adenosine triphosphate ...
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bioRxiv - Immunology 2021Quote: ... and CD19 and the viability dye 7-aminoactinomycin-D (catalog #s 340662, 641407, 340692, 341103, 559925, respectively, BD Biosciences, San Jose, CA). The gating strategy is shown in Figure S1 ...
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bioRxiv - Immunology 2021Quote: ... and apoptotic DCs after treatments were determined by flow cytometry using surface Annexin V detection and 7-Amino-actinomycin D (7-AAD) incorporation (BD Biosciences, USA). DCs were washed with PBS ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
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bioRxiv - Systems Biology 2023Quote: ... all strains are grown in YPD (2% w/v Bacto Peptone, BD Biosciences #211677; 2% w/v D-glucose, BD Biosciences #215520; 1% w/v Yeast Extract, BD Biosciences #212750) at 30°C.
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bioRxiv - Immunology 2021Quote: ... or PMA + ionomycin in the presence of brefeldin A for 4 h and stained using the Cytofix/Cytoperm Kit (BD Bioscience, Franklin Lakes, NJ, USA). Foxp3-positive cells and apoptotic cells were detected using the BD Foxp3 Staining Kit (BD Bioscience ...
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bioRxiv - Systems Biology 2023Quote: ... All strains were reconstituted and grown anaerobically (6% H, 20% CO2, 74% N2) at 37°C in liquid Brain Heart Infusion medium (BHI; BD Bacto Ref:237500 Lot:1194923) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biophysics 2024Quote: ... for 24 h to induce protein production previous to fluorescence-assisted cell-sorting (FACS) on a MelodyTM Cell sorter (BD Biosciences, Franklin Lakes, NJ, USA) for transgene expression.
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bioRxiv - Developmental Biology 2024Quote: ... The culture medium was supplemented with 10 nM JF646-Halo (fluorescent HaloTag-ligands, made in-house)81 for 1 h before cell-sorting by FACS (BD FACSAria II SORP, Becton Dickinson). Cells positive for both JF646 and mScarlet-I signals were collected ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then washed in 1X AnnexinV buffer and stained with AnnexinV and 7-amino-actinomycin D (7-AAD) according to the manufacturer’s protocol (BD Pharmingen, San Diego, CA). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for ON in primary antibody (SOX2, R&D Systems, MAB2018; TBR2, Abcam, ab23345; HOPX, Santa Cruz, sc398703; HOPX, Proteintech, 11419-1-AP; N-cadherin, BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Cells were seeded at 50,000 cells/well in a poly-d-lysine coated 96-well black well/clear bottom plate (BD Falcon, Franklin Lakes, USA) and cultured overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... FGF10 expression was induced by the addition of isopropyl-1-thio-B-D-galactopyranoside to RosettaTM2(DE3)pLysS carrying pET21d-FGF10 in 2x YT medium (BD Biosciences, Cat#244020) with Carbencillin (50 µg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Antibody-stained cells were washed in FACS staining buffer and resuspended in 20 µL 7-actinomycin D (7-AAD, BD Pharmigen flow kit) for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... oxysporum conidia were isolated after growth for 3-4 d in potato dextrose broth (PDB; Becton, Dickinson and Company, Le Pont de Claix, France) by filtering through two layers of Miracloth® (Merck Millipore ...
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bioRxiv - Microbiology 2023Quote: ... The cytokine releases were evaluated by ELISA in cell supernatants or in peritoneal fluids (IL-1β, IL-12, IL-6, TNFα, TGFβ, IL-10 BD Biosciences, R&D Systems). 8 isoprostane ...
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bioRxiv - Genetics 2023Quote: ... containing penicillin/streptomycin (50U/ml) and 10% FBS and plated onto 12mm Poly-D-Lysine/Laminin pre-coated coverslips (BD Biosciences, Franklin Lakes, NJ). Cells were incubated overnight at 37°C and imaged the following day (sixteen to twenty-four hours after plating cells ...
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bioRxiv - Cancer Biology 2023Quote: ... after which the cytotoxicity towards IRF8 WT or KO lymphoma cells was assessed by FACS using 7-aminoactinomycin D (7-AAD) (BD Biosciences, Cat# 51-68981). Controls of non-OVAp pulsed B cell lymphomas were included in all instances ...
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bioRxiv - Immunology 2024Quote: The effects of protein fermentation compounds on cell viability were assessed by calculating the intensity of the cellular fluorescence produced from 7-Aminoactinomycin D (7-AAD) using a BD FACS Diva flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). In brief ...
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bioRxiv - Molecular Biology 2020Quote: ... or mouse monoclonal antibodies (eIF2α clone D-3, Santa Cruz #sc-133132, β-Actin clone E4D9Z, Cell Signaling Technology #58169; Phosphoserine/threonine clone 22A, BD Transduction Laboratories # 612548) were added to the blocking solution at dilutions ranging from 1/500 to 1,5000 according to the manufacturer’s datasheets ...
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bioRxiv - Immunology 2020Quote: ... KC and IL-6 in the cell culture supernatants and lung homogenates were determined by commercialized ELISA kit (IL-1β and KC were from R&D Systems BD, IL-6 was from Biolegend) and SAA3 in the lung homogenates and plasma were determined by ELISA kit (MilliporeSigma ...