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Citations for Becton, Dickinson and Company :
351 - 400 of 442 citations for Borrelia burgdorferi sensu stricto B31 Surface Lipoprotein P27 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and 2 mM KH2PO4)-FBS 5% for 15 min and then stained for surface expression with fluorophore-marked antibodies against SLAMF1 (BD, 559572), CD31 (BD ...
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bioRxiv - Immunology 2021Quote: PBMCs were stained for surface markers using combinations of the following directly conjugated monoclonal antibodies: anti-CCR7–BV650 (clone 3D12; BD Biosciences), anti-CCR7–PE-Cy7 (clone 3D12 ...
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bioRxiv - Immunology 2021Quote: ... The expression of the standard activation markers CD69 and CD134 (OX40) was quantified on the cell surface using anti-CD69–FITC (clone L78; BD Biosciences) and anti-CD134–BV711 (clone ACT35 ...
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bioRxiv - Immunology 2020Quote: ... for the last 4hrs before subjecting for fluorochrome-conjugated antibody staining for surface markers and intracellular cytokine (Perm/Fix buffer, BD Biosciences). Cells were analyzed by 24-color compatible full-spectrum Cytek Aurora flow analyzer (College of Veterinary Medicine ...
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bioRxiv - Immunology 2020Quote: ... a combination of the following anti-mouse antibodies was used to stain for surface markers (all from BioLegend, eBioscience, or BD Biosciences): anti-CD3ε (145-2C11) ...
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bioRxiv - Microbiology 2021Quote: ... Leaf press cultures were done by placing birch leaves on the surface of a plate containing 0.2x potato dextrose broth (PDB; BD Difco; www.fishersci.com) with 15g/l agar (PDA) ...
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bioRxiv - Immunology 2020Quote: ... Purified MDSCs from spleen and bone marrow cells were isolated using a mojosort magnet followed by cell surface staining with CD11b and Gr1 monoclonal antibodies for cell sorting on BD FACS Aria III cell sorter (BD Biosciences). Similarly ...
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bioRxiv - Immunology 2020Quote: ... CD138 (plasma cell marker) and other B cell surface molecules on a flow cytometer (BD LSR II™ or Celesta™). To analyzed immune cells in vivo ...
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bioRxiv - Immunology 2020Quote: ... IFNγ-producing cells were stained with IFNγ Detection Antibody in conjunction with cell surface markers (listed below) and analyzed on an LSRFortessa flow cytometer (BD Biosciences). Antibodies used included CD3e (145-2C11) ...
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bioRxiv - Immunology 2021Quote: ... Spike -specific T cells were measured by surface staining followed by fixation and permeabilization using BD fixation/permeabilization solution kit (BD Biosciences,) and intracellular cytokine staining (table S1) ...
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bioRxiv - Immunology 2020Quote: ... Fc blocking and fluorescent staining was performed as previously described and samples sorted into pure cell populations based on cell surface marker expression using a FACS Fusion (BD Biosciences). For data where peritoneal populations are expressed as cells/ μl ...
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bioRxiv - Immunology 2020Quote: ... Up to 5×106 cells were washed in cold FACS wash (PBS supplemented with 2% FCS and 2mM EDTA) prior to being stained with a surface receptor antibody cocktail containing FcBlock (BD, 2.4G2) and LIVE/DEAD fixable blue dead cell stain (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... cells were stained for surface antigens as above and then fixed and permeabilised with the Transcription Factor buffer set (562725, BD Biosciences). Next cells were incubated with 4M HCL for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: Flow cytometry analysis was performed using the following cell surface markers: anti-CD11b conjugated to phycoerythrin (PE, BD Biosciences, clone ICRF44) and anti-CD44 allophycocyanin (APC ...
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bioRxiv - Immunology 2022Quote: ... DCs were analyzed for expression of maturation markers on the cell surface by flow cytometry using an LSR II (BD Biosciences) and FlowJo v10.1 software (University of Washington Cell Analysis Facility).
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bioRxiv - Immunology 2022Quote: ... Cell subsets for the populations from different donors were labelled simultaneously with the cell surface sorting panel (Table S4) an oligonucleotide-tagged antibody sample tag (BD Biosciences) prior to cell sorting on a FACSAria3 (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. The surface expression level of S proteins (Fig. 3d) was measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... we first labeled cell surface proteins in FACS buffer for 30 minutes before washing with 1x PBS and fixation with BD Cytofix/Cytoperm (BD Biosciences) for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. The surface expression level of S proteins (Fig. 3d) was measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs were stained with a panel of fluorochrome-conjugated antibodies reactive with the following cell surface markers: CD45-APC/Cy7 (clone 30-F11; BD Biosciences), CD3-PE/Cy7 (clone 500A2 ...
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bioRxiv - Biophysics 2023Quote: ... Enrichment of surface expression in PE-selected cells was confirmed by flow cytometry using a BD FACS LSR II cytometer (BD Biosciences) and analyzed with FlowJo software (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... was used as a secondary antibody. The surface expression levels of S proteins (Fig. 4A) were measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (FlowJo LLC) ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then stained with live/dead and cell surface markers in MACS solution (Miltenyi) supplemented with 2% BSA (Miltenyi) prior to permeabilization with Cytofix/Cytoperm (BD Biosciences) and staining with intracellular markers in Perm/Wash (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: Cells were surface stained with fluorochrome-conjugated mAbs to examine the expression of the phenotypic markers CD3 (clone UCHT1, BD Pharmingen), CD4 (RPA-T4 ...
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bioRxiv - Immunology 2024Quote: ... surface antibodies were incubated with cells for 30 minutes at 4°C in hFACS buffer and Brilliant Stain Buffer (BD Biosciences). For intracellular staining ...
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bioRxiv - Immunology 2023Quote: ... A combination of the following cell surface markers were subsequently added and cells were stained at room temperature for 15 min: CD3-BUV496 (clone UCHT1, BD Biosciences), CD14-PE-Cy5 (clone 61D3 ...
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bioRxiv - Bioengineering 2024Quote: ... Viability dye was quenched by washing with FACS buffer (PBS + 2% FBS + 1 mM EDTA) before addition of surface staining antibodies in a 1:1 dilution of Brilliant Stain Buffer (BD 563794) in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... cells were first stained for surface markers and later stained for intracellular molecules following fixation and permeabilization following the manufacturer’s protocols (BD Cytofix/Cytoperm). For phosflow ...
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bioRxiv - Immunology 2023Quote: ... Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail, BD Biosciences) and added to the cells ...
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bioRxiv - Immunology 2023Quote: ... and ionomycin (500 ng/mL) for 3 hrs at 37°C prior to surface and intracellular staining using the BD Cytofix/Cytoperm Kit (BD Biosciences). Intracellular transcription factor staining was performed using a Foxp3 staining kit (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... Fc blocking and fluorescent staining were performed as previously described and samples sorted into pure cell populations based on cell surface marker expression using a FACS Aria Fusion (BD Biosciences).
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bioRxiv - Physiology 2024Quote: ... Small intestine lamina propria cells from male C57BL/6J mice were prepared and stained with surface molecules for 30 min at 4 followed by sorting on a FACS Aria III cell sorter (BD Biosciences). A gating strategy of live Lineage−CD127+KLRG1-c-Kit+ was used to sort the ILC3s.
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bioRxiv - Bioengineering 2024Quote: ... and stained for 30 min on ice with surface antibodies at a 1:200 dilution in a 1:1 mixture of FACS buffer and Brilliant Stain Buffer (BD Biosciences). Cells were washed with FACS buffer and PBS and fixed with 2% paraformaldehyde on ice for 20 min ...
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bioRxiv - Immunology 2024Quote: ... the enriched CD4+ T cell suspension was surface stained and Tregs and Tconv were further purified by cell sorting with a FACSAria II (BD Biosciences) according to the following phenotype ...
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bioRxiv - Immunology 2023Quote: ... 0.25X and 4X concentration of CD4 T cell-depleting antibody for 30 minutes at 37°C before surface staining with CD3 (clone SP34-2, BD Pharmingen), CD4 (Clone L200 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... samples were taken from surface water from multiple points of Laguna Intermedia using sterile BD Falcon vials (BD Biosciences, San Jose, CA). Isolates were obtained by plating 200 μl of each sample on Pseudomonas Isolation Agar (PIA ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for surface markers (when required) then fixed/permeabilized by using PE Active Caspase-3 Apoptosis Kit (BD Bioscience, 550914) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were first surface stained for GC markers then washed twice in staining buffer and treated with fixation/permeabilization solution Cytofix/Cytoperm (BD Bioscience, 554722) for 30 minutes on ice in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... was added after 1 hour and cells were stained for surface NK makers (CD56 and CD16) and intracellular IFN-γ (Cytofix/Cytoperm; BD Biosciences).
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bioRxiv - Bioengineering 2021Quote: ... To assess activation marker status T cells were stimulated with 0.1 μg/mL of D117C or PIGF on collagen coated plates for 16-20 hr and surface-stained with anti-human CD3 BUV395 (BD clone SK7), CD4 BV785 (Biolegend clone RPA-T4) ...
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bioRxiv - Bioengineering 2021Quote: ... to assess T cell phenotype in response to EcN lysate cells were similarly stimulated and surface stained with CD3 BUV395 (BD clone SK7), CD4 BV785 (Biolegend clone RPA-T4) ...
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bioRxiv - Cell Biology 2021Quote: ... The number of CD19 molecules expressed on the cell surface was estimated by flow cytometry in combination with the BD Quantibrite kit (BD Biosciences, #340495) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... placed on the surface of a 60mm tissue culture dish coated by Matrigel basement membrane (MBM) (BD Bioscience, San Jose, California, USA) reconstituted in Coon’s medium (1:2) ...
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bioRxiv - Microbiology 2021Quote: ... Wild-type Sodalis glossinidius morsitans were isolated from surface-sterilized Gmm pupae and plated on Difco™ Brain Heart Infusion Agar (BD Biosciences) plates that were supplemented with 10% bovine blood (BBHI) ...
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bioRxiv - Immunology 2022Quote: ... at 4°C for 5 min before surface staining with a cocktail of the following antibodies for 20 min at 4°C: CD3 V450 (Clone 17A2) (BD Cat. 561389), CD4 BV750 (Clone H129.19 ...
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bioRxiv - Immunology 2021Quote: ... and apoptotic DCs after treatments were determined by flow cytometry using surface Annexin V detection and 7-Amino-actinomycin D (7-AAD) incorporation (BD Biosciences, USA). DCs were washed with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellet was then stained with antibodies to microglial cell surface markers (CD11b-PE Cy7, 1:200 Biolegend, # 101216; CD45-APC Cy7, 1:200, BD Biosciences # 103116;) for isolation using the FACSAria™ III cell sorter (Fig 1C) ...
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bioRxiv - Immunology 2020Quote: ... All antigen specific CD8 T cells were phenotypically analyzed by Flow Cytometry after surface staining using monoclonal antibodies for Thy1.1(BD clone OX-7), CD45.1 (Biolegend clone A20) ...
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bioRxiv - Immunology 2021Quote: ... Cells were filtered and cell surface staining was performed using antibodies specific for CD3ε (eBioscience, clone145-2C11) and Vγ5 (BD Pharmingen, clone 536).
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 30 min with the following cell surface antibodies: BUV661-anti-CD45 (D058-128, BD Biosciences 741657 Lot 0198214), PE-Cy7-anti-CD20 (2H7 ...