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Citations for Becton, Dickinson and Company :
351 - 400 of 1463 citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... T cells were 2-way sorted from PBMC pre-gated on CD4+ CD14− viable lymphocytes using a FACS Aria (BD) and the antibodies listed in supplementary material procured from Biolegend or BD ...
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bioRxiv - Immunology 2020Quote: RAW macrophages expressing t-Epac-vv were imaged using a BD Pathway high-content imaging inverted widefield microscope (BD biosciences) equipped with a 20X 0.75 N.A ...
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bioRxiv - Immunology 2020Quote: ... with additional staining of CCR9 or CCR4 were used to apply t-SNE analyses for ILC1-like cells with 500 iterations using FlowJo software (BD).
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bioRxiv - Cancer Biology 2022Quote: ... 6,000 CD45+CD19-TCRβ+ cells (i.e., T cells), and 20,000 CD45+CD19-TCRβ- cells (e.g., neutrophils) using FACS Aria (BD Bioscience, NJ, USA). The obtained mixture of cells was subjected to single-cell transcriptomics.
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bioRxiv - Cancer Biology 2022Quote: ... is the complete list of antibodies used for experiments of co-culture between T cells and AML cell lines: CD8 PE-Cy7 (cat#557746 from BD), CD4 FITC (cat#980802 from BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... and T lymphocytes were stained using antibodies against CD4 and CD8 (1:100, catalog nos. 553049 and 553032; BD Biosciences). At least 1◻×◻105 leukocytes per mouse were analyzed and their amount per microliter of blood was calculated.
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bioRxiv - Immunology 2022Quote: ... Live (PI-) conventional (YFP-) and regulatory (YFP+) CD4+TCRβ+ T cells were sorted with a BD FACS Aria (BD Biosciences) to >95% purity ...
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bioRxiv - Immunology 2023Quote: ... Cells were harvested after 48h co-culture with T cells and stained in PBS with Fixable Viability Stain 575V (BD) or Fixable Viability Dye eFluor™ 506 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... The isolated cells were further purified to obtain naïve CD4+ T cells (CD4+ CD25-CD44-) by cell sorting using a FACSAria II (BD Biosciences) instrument (see S1 Table for antibodies specifications) ...
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bioRxiv - Immunology 2024Quote: ... Treg cells (CD4+Foxp3GFP+CD25+GITR+) and naïve CD4+ T cells (CD4+CD62L- cells) were sorted using an Aria Fusion sorter (BD Biosciences) with a 70μm nozzle ...
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bioRxiv - Immunology 2024Quote: ... T cells or NK cells were mixed with tumor cells for 6 h in the presence of GolgiPlug (BD, BDB555029). As a positive control ...
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bioRxiv - Immunology 2024Quote: ... Individual tetramer-labeled cells were isolated for TCR sequencing or T cell cloning by index sorting using the purity mode on FACS Aria (BD).
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bioRxiv - Immunology 2024Quote: ... BrdU incorporation was assessed in splenic T cells 24 hours after injection using a BrdU flow cytometry kit (BD PharmingenTM).
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bioRxiv - Immunology 2024Quote: ... cells were incubated for 5 hr with and without 50ng/mL phorbol 12-myristate 13 acetate (PMA) and 1μg/mL ionomycin to stimulate cytokine production in T cells as well as GolgiPlug (BD Biosciences) to retain cytokines in the cytoplasm ...
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bioRxiv - Immunology 2019Quote: ... HSPCs were stained using c-Kit-APC (2B8; BD Biosciences), Sca-1-Cy7PE (D7 ...
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bioRxiv - Cell Biology 2019Quote: ... and anti-cytochrome C 6H2.B4 (BD Pharmingen, cat# 556432).
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bioRxiv - Microbiology 2022Quote: ... coli was cultured at 37°C in Luria-Bertani (BD) broth or agar (1.5% (w/v) ...
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bioRxiv - Molecular Biology 2022Quote: ... C-Raf (610152) PARP (556362) and ITCH (611199) from BD transduction laboratories ...
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bioRxiv - Immunology 2020Quote: ... 4°C with Brilliant Violet Buffer (BD Biosciences, Cat# 566349) at 1/4 in PBS-FBS 1% (see Supplemental Table 3 for antibody staining panel).
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bioRxiv - Cell Biology 2021Quote: ... κ isotype-PE-Cy7 (1:200, LOU/C, BD Biosciences). Cells were then stained with LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... at 4 °C before flow cytometry on a FacsCanto (BD). Data was analyzed using FlowJo software (Version 10 ...
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bioRxiv - Microbiology 2022Quote: ... were aerobically grown at 37 °C in Luria-Bertani (BD) with 10 µg/mL tetracycline.
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bioRxiv - Cell Biology 2022Quote: ... Anti-Elongin C antibody (610761, 1:3000) was from BD Biosciences ...
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bioRxiv - Physiology 2023Quote: ... and co-stained with anti-Cytochrome c (BD Biosciences, 556432) and anti-CD81 (Cell signaling ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C in brain heart infusion (BD Life Sciences) supplemented with 0.5% yeast extract and 0.1% cysteine (BHIS ...
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bioRxiv - Cell Biology 2022Quote: ... the protein transport inhibitor was added (BD GolgiStopTM ...
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bioRxiv - Neuroscience 2020Quote: ... Human Soluble Protein buffer kit (BD Biosciences) was used in conjunction with flex sets for detecting granulocyte-macrophage colony-stimulating factor (GM-CSF) ...
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bioRxiv - Immunology 2021Quote: ... and protein transport inhibitor cocktail (BD, USA) for 5h ...
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bioRxiv - Immunology 2024Quote: ... GolgiStop Protein Transport Inhibitor (BD Bioscience, UK) was added ...
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bioRxiv - Immunology 2021Quote: ... Spike protein conjugated to Alexa 647 and Spike protein conjugated to Alexa 488 and Brilliant Staining buffer (BD Horizon). Samples were then incubated on ice for an additional 30 minutes before they were washed twice with P2 and fixed in a final concentration of 1% paraformaldehyde for 15 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... AIM assay was conducted as previously explained and CD69+CD40L+CD4+ T cells were live-sorted on a FACS Aria cell sorter (BD BioSciences) equipped for handling of biohazardous material ...
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bioRxiv - Immunology 2022Quote: The following mouse anti-human antibodies were used for purification of CD8 T cell subsets by Flow Cytometry: anti-CD45RO PE CF594 (clone UCHL1, BD Biosciences), anti-CD56 PE Cy7 (clone B159 ...
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bioRxiv - Immunology 2019Quote: ... Memory CD4 T cell sorting (see gating strategy Figure supplement 1A) was performed on a BD Aria III/Fusion cell sorter (BD Biosciences). 100,000 memory CD4+ T cells were sorted into TRIzol LS reagent (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Total T cells (1 × 106) were activated with plate bound α-CD3ε (OKT3, Biolgend) plus α-CD28 (CD28.2 RUO, BD Biosciences) at 5µg/mL each or corresponding isotype controls for a total of 72 hours ...
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bioRxiv - Immunology 2021Quote: Macromolecular-centered free radicals were detected after stimulation of purified NOD and NOD.Ncf1m1J CD8+ T cells with α-CD3 (clone 145-2C11 (BD Pharmingen); 1μg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... An antibody panel for macrophage and T cell polarization was also stained for consisting of: F4/80 BUV395 (T45-2342, BD Biosciences), CD86 BV786 (GL-1 ...
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bioRxiv - Systems Biology 2020Quote: ... Naive CD4+T cells sorting of CD4+CD45RA+CD45RO-CD25- and memory CD4+ T cells sorted as CD4+CD45RA- CD45RO+CD25- had a purity of over 99% with a FACSAria (BD Bioscience).
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bioRxiv - Immunology 2022Quote: ... T cell receptor Vβ chains of splenic CD4+ T cells from CKO and control mice were assessed using a commercial mouse TCR Vβ screening panel (BD Biosciences). Flow cytometry data collection was performed on an LSR II (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 5-6 days and CFSE dilution of T cells was determined on a BD Canto II (BD Bioscience) and analyzed by FlowJo (v10.1 ...
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bioRxiv - Immunology 2021Quote: ... the wells were washed six times with DPBS containing 0.05% (v/v) Tween-20 (PBS-T 0.05%) and blocked with 3% (w/v) Difco Skim Milk (cat. n° 232100, BD Biosciences, USA) in PBS-T 0.05% for 2 h in agitation at RT ...
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bioRxiv - Immunology 2020Quote: ... Live CD3+CD4+CD45RA− activated CD154+ and resting CD154−CD69− T cells were sorted with a FACSAria II instrument (BD Biosciences). Sorted T cells were lysed in Buffer RLT Plus (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... Performance of the cell sorter was checked daily by the flow cytometry core at La Jolla Institute with the use of CS&T beads (BD Biosciences), and PMT voltages were manually adjusted for optimum fluorescence detection on each experiment day ...
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bioRxiv - Immunology 2021Quote: Mononuclear cells were directly labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, clone, FITC anti-mouse CD4, APC-Cy®7 anti-mouse CD8, for T lymphocyte phenotype, all from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... the wells were washed six times with PBS containing 0.05% (v/v) Tween-20 (PBS-T) and blocked with 3% (w/v) Difco Skim Milk (BD Biosciences, USA) in PBS-T for 2 h in agitation at RT ...
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bioRxiv - Biochemistry 2021Quote: ... cells (0.1×106 T cells in 100 μL PBS) were left untreated or were stimulated with anti-CD3 antibodies (145-2C11 (BD Bioscience) or OKT3 (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... Eventually 200 000 CD8+ T cells from vaccinated animals were seeded with 50 000 ovalbumin pulsed splenocytes on IFNγ ELISpot assay plates (BD Biosciences). The cells were incubated for 48 hours and the plates were developed as described by the manufacturer.
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bioRxiv - Immunology 2019Quote: ... Intracellular cytokine staining was performed to examine the production of cytokines in these CAR-T cells using Fixation/Permeabilization Solution Kit (BD Biosciences). CD107 expression was determined as described (Liu et al. ...
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bioRxiv - Microbiology 2019Quote: ... B and T-lymphocytes and CD11c+ phagocytes were sorted on a FACSAria IIu Flow Cytometry Sorter (BD Biosciences, San Jose, CA) and into 0.22 µm Spin X centrifuge tube filters (Corning ...
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bioRxiv - Immunology 2020Quote: ... Total CD4+ T cells were isolated by negative magnetic selection using a CD4 T cell isolation kit (Stemcell) and CD4+CD25hiCD127lo-neg Treg cells were sorted on a FACS Aria (BD Biosciences). Treg cells were cultured in RPMI 1640 medium supplemented with 5% Human serum ...
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bioRxiv - Molecular Biology 2019Quote: ... 20μg of total protein was loaded per lane and probed with the following primary antibodies dissolved in TBS-T: Topoisomerase IIβ (1:1000, overnight) (BD Biosciences), αTubulin (DMIA ...