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Citations for Becton, Dickinson and Company :
351 - 400 of 655 citations for 7 Methoxy 6 azaindole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Samples of stimulated CD45.2+ OT-I cells and CD45.1+ wild type splenocytes mixtures were stained with CD8a and CD45.2 antibodies and 7-AAD and OT-I cells were sorted (BD FACSAria) into 100% FBS and pelleted.
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bioRxiv - Microbiology 2021Quote: ... the splenocytes were stained with the extracellular markers PE-Cy ™ 7 Hamster Anti-Mouse CD3e (Cat. # 552774, BD Pharmingen), APC-Cy7 Rat Anti-Mouse CD4 (Cat ...
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bioRxiv - Genetics 2020Quote: ... we also collected 5-7 ml of peripheral blood in a tube containing the anticoagulant K3 EDTA (#6457, BD Biosciences) which was used for flow cytometry as described below ...
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bioRxiv - Genomics 2021Quote: Each strain was cultivated for 3-7 days in 10 mL of Tryptic Soy broth (BD Diagnostic, catalog no. 211768) with 0.5% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-F4/80-PE clone REA126 and human anti-CD14-FITC from Miltenyi Biotech and 7-AAD staining solution from BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... The cell viability dye 7-AAD (Via-Probe) and Live/dead fixable Aqua fluorescent reactive dye were purchased from BD Biosciences and ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Images were analysed using Cellprofiler80 with default settings to quantify the cytoplasmic to nuclear intensity ratios.The following antibodies were used for IF: p53 (DO-7 FITC conjugate, BD biosciences), USP28 (Bethyl A300-898A) ...
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bioRxiv - Cell Biology 2022Quote: ... cultured PAECs were incubated with 2’,7’–dichlorofluorescin diacetate (DCFDA; 1μM) for 10 minutes at 37°C and then assessed by flow cytometry (BD LSRFortessa).
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bioRxiv - Immunology 2022Quote: ... HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml, BD Biosciences) and CD28 (1 μg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were then diluted 1:1 with ice cold 0.5% BSA/CMFNM and stained with 60 µg/ml 7-AAD (BD, 559925) for more than 20 min on ice ...
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bioRxiv - Immunology 2024Quote: ... at which point they were phenotyped (FOXP3-PeCy7, clone FJK-16s, Thermo Fisher Scientific; HELIOS-PEDazzle594, clone 22F6, Biolegend; Thy1.1-BV711, clone OX-7, BD Biosceinces ...
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bioRxiv - Bioengineering 2023Quote: ... Mice sera were collected retro-orbitally at 4 h and 7 days post administration and subjected to cytokine analysis (Cytometric Bead Array Mouse Inflammation Kit, BD) using flow cytometry following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... containing 10 μM Y27632 (Wako) on 6-well plates coated with Matrigel (BD Biosciences). The cells were plated at 4.0 × 105 cells ...
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bioRxiv - Pathology 2019Quote: ... CD127 (HIL-7R-M21) and HLA-DR (G46-6) antibodies were purchased from BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Levels of human IL-6 were determined by enzyme-linked immunosorbent assays (BD Biosciences) as per the manufacturers’ instructions.
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bioRxiv - Cell Biology 2020Quote: ... Myogenic differentiation was initiated 6 hrs after seeding cells onto Matrigel (BD Life Sciences) coated cell culture dishes (30,000 cells/cm2 ...
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bioRxiv - Immunology 2020Quote: HyIL-6 (Fischer et al., 1997) cloned into the pAcGP67-A vector (BD Biosciences) in frame with an N-terminal gp67 signal sequence and a C-terminal hexahistine tag was produced using the baculoviral expression system ...
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bioRxiv - Developmental Biology 2020Quote: Nucleated bone marrow cells were plated into tissue culture 6 well plates (BD Falcon) at a density of <105 cells/cm2 ...
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bioRxiv - Bioengineering 2020Quote: ... singularized and seeded on a 2D collagen coated 6-well plate (BD, Cat. # 356400). Cells were fed with mammary gland specific media (Promocell ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated with primary antibodies: anti-Ccnd1 (monoclonal DCS-6, BD biosciences, 1:500), anti-Gabra4 (polyclonal ...
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bioRxiv - Cell Biology 2022Quote: ... containing inactivated Mycobacterium tuberculosis H37Ra (6 mg/mL, BD B231141, Cat. DF3114-33-8). Mice were also co-injected with pertussis toxin (200 ng/mouse in 1X PBS ...
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bioRxiv - Cell Biology 2022Quote: ... MOG was emulsified in complete Freund’s adjuvant (CFA BD B263910, Cat. DF0639-60-6) containing inactivated Mycobacterium tuberculosis H37Ra (6 mg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... transduced with a lentiviral vector encoding for luciferase in 1:6 matrigel (BD Biosciences) were orthotopically injected in 5-week-old NSG mice (Charles River Laboratories) ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were resuspended in ice cold PBS and supplied with 10 μl of 7-AAD to assess cell viability (BD Pharmigen) and sorted with a BD Aria III FACS as previously described 30 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Adherent and suspension cells of each sample were pooled and after centrifugation for 5min at 800 x g cells were carefully resuspended in cell culture medium with 10 µl/ml 7-AAD (BD Biosciences). After 10 minutes incubation in the dark cells were immediately analyzed by flow cytometry using a FACS Aria III (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... washed twice and resuspended in 200 μL of blocking buffer in the presence of 7-AAD (BD Biosciences Cat#: 51-68981E) or DAPI (Invitrogen Cat# ...
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bioRxiv - Cell Biology 2022Quote: ... Cell survival was measured by FACS analysis after staining cells with a membrane-impermeable DNA binding dye (DAPI, PI, or 7-AAD) and/or annexin V (BD Pharmingen).
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bioRxiv - Developmental Biology 2022Quote: ... Cells from mouse tail skin (aging RNA-seq) and dorsal/ventral skin (fibulin 7 RNA-seq) were isolated using FACS Aria flow cytometer (BD Biosciences). Data were analyzed using FlowJo software (BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... Myoblasts were stained with 7-AAD (cell viability) and with the positive selection marker anti-CD56 AF647-conjugated (clone R19-760, BD biosciences). Of note ...
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bioRxiv - Immunology 2020Quote: ... The cells were then stained with 7-AAD (live/dead marker) prior to flow cytometric sorting on an FACS Aria II sorter (BD Biosciences). The sorted germinal centre B cells (7-AAD−/B220+/GL-7+/Fas+ ...
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bioRxiv - Immunology 2020Quote: ... A fixable Viability Dye (APC-eFluor780 1:200, 1:400) (eBioscience, Germany) or ViaProbe (7-AAD, 1:33) (BD Biosciences, Germany)) was used for live/dead discrimination ...
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bioRxiv - Immunology 2021Quote: ... and then labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, FITC anti-mouse CD4, APC-Cy®7 mouse anti-CD8, all from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: Mononuclear cells were directly labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, clone, FITC anti-mouse CD4, APC-Cy®7 anti-mouse CD8, for T lymphocyte phenotype, all from BD Biosciences ...
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bioRxiv - Genomics 2020Quote: ... ODInCas9 system was left to turn off for 7 days post transfection before assessment using FACS instrument BD LSRFortessa (BD Bioscience) and confocal microscopy instrument Cell Voyager 7000S (CV7000S ...
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bioRxiv - Immunology 2019Quote: ... NK92 cells or primary patient fibroblasts were incubated with BrdU for 12 hours prior to fixation and staining with 7-AAD (BD Biosciences). Cells were analyzed via FACS on a BD Fortessa with 18-color configuration and resulting data were evaluated using Kaluza (Beckman-Coulter ...
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bioRxiv - Microbiology 2021Quote: ... The infectivity was measured by detecting GFP fluorescence using a Zeiss fluorescence microscope (AXIO observer 7) and measured as the percentage of GFP positive cells analyzed with a Fortessa X20 flow cytometer (BD Biosciences) at 24 hours post infection ...
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bioRxiv - Immunology 2020Quote: ... then washed with 2% FCS - PBS and stained with Mouse anti-IRF-7-PE (pS477/pS479, clone K47-671; BD Biosciences) or Mouse anti-NF-κB p65-Alexa Fluor 647 (pS529 ...
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bioRxiv - Cell Biology 2021Quote: ... macrophages were incubated with 5 μM 2’, 7’ dichlorofluorescein diacetate (DCFDA, Invitrogen at 37°C for 30 min, followed by acquisition using FACS Canto I (BD Sciences) within 30 minutes of staining ...
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bioRxiv - Cell Biology 2019Quote: ... Cells with normal mCherry fluorescence (mid 70-80% fluorescence) and top or bottom ~7% eGFP fluorescence (~4 million cells/population) were sorted using a BD FACSAria III (BD Biosciences) and collected into 15 ml polypropylene tubes (Cellstar ...
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bioRxiv - Immunology 2020Quote: ... Spleen B cells were analyzed for BrdU incorporation (by anti-BrdU mAb staining) and DNA contents (by 7–AAD staining after cells were permeabilized) using the APC BrdU Flow kit® (BD) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Microbiology 2021Quote: ... Infectivity was assessed by detecting GFP fluorescence using a Zeiss fluorescence microscope (AXIO observer 7) and/or determined by the percentage of GFP(+) cells analyzed using a Fortessa X20 flow cytometer (BD Biosciences) at 16 ...
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bioRxiv - Immunology 2022Quote: ... Human CD34+ stem cell purity was monitored by anti-human CD34 FITC (clone:581) and 7-AAD viability dye using LSR Fortessa X-20 flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 7 days and immunostained for flow cytometry (Antibodies: CD34, clone 581; CD117, clone: 104D2; CD11b, clone ICRF44 (BD Pharmingen) and 1:50 FcR Blocking Reagent (Miltenyi Biotec)) ...
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bioRxiv - Biochemistry 2023Quote: Cells were grown in synthetic complete medium at 25°C to mid-log phase after 7-8 doublings and harvested for direct fluorescent measurement of RFP and YFP channels by an LSR Fortessa (BD biosciences) flow cytometer ...
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bioRxiv - Cancer Biology 2023Quote: Apoptosis was measured by evaluating the activation of caspase 3/7 using the Ac-DEVD-AMC Caspase-3 Fluorogenic Substrate (BD Biosciences) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 5 × 106 MCF-7 cells stably expressed shCtrl and shMYSM1 lentivirus were suspended in 100 μl of culture medium/Matrigel (1:1) (BD Biosciences) and implanted subcutaneously into the bilateral armpits of 4-week-old female BALB/c nude mice (Vital River Laboratory) ...
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bioRxiv - Developmental Biology 2023Quote: ... or (ii) PE-Annexin V conjugated antibody (1:33 dilution in TBST) and 7-AAD as per kit instructions (BD Pharmingen). Samples were analysed by flow cytometry within 30 min.