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Citations for Becton, Dickinson and Company :
351 - 400 of 502 citations for 7 12 Dioxa spiro 5.6 dodec 9 ene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were collected and stained with BrdU-APC and 7-AAD using BD Pharmingen BrdU Flow Kit (BD, 552598) following manual instructions ...
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bioRxiv - Immunology 2019Quote: ... Cells were resuspended in 0.5ml of Stain Buffer (FBS) and incubated with the viability dye 7-AAD (BD Biosciences) at RT for 10 minutes prior to acquisition on a 3-laser ...
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bioRxiv - Physiology 2020Quote: ... the pellet was resuspended in 7 mL and filtered (Cell Strainer 70 um Nylon; BD Falcon, Bedford, MA, USA). The islets in the filter were washed twice with HBSS and plated in a RPMI solution by inverting the filter (RPMI ...
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bioRxiv - Cancer Biology 2022Quote: ... Apoptosis was quantified using allophycocyanin-conjugated Annexin V and viability was assessed with 7-Amino-actinomycin D (BD Bioscience). Gating was defined using untreated/unstained cells and treatments were evaluated at 20,000 counts using BD FACSVantage SE.
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bioRxiv - Molecular Biology 2023Quote: ... A small aliquot of cells were stained in PBS 1x with the viability marker 7-AAD (BD Biosciences, 559925) and T-cell marker CD4 FITC (clone GK1.5 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were stained with FITC-conjugated Annexin V and 7-AAD according to the manufacturer’s instructions (BD Biosciences) and analysed using a LSRFortessa X-20 cytometer and FlowJo software (RRID:SCR_008520 ...
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bioRxiv - Bioengineering 2022Quote: ... Hybridization of 750ng of cRNA on Human HT-12 v4.0 Expression BeadChip (Illumina, BD-103-0604), were performed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Whole blood was collected from 8-12 weeks old mice in EDTA microvettes tubes (BD Biosciences) and complete blood count were analyzed using ADVIA 2120i Hematology systems (Siemens Healthineers ...
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bioRxiv - Genomics 2022Quote: ... coli K-12 cells were pre-cultured in Luria-Bertani (LB) medium (1.0% Bacto tryptone [BD Biosciences ...
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bioRxiv - Cancer Biology 2019Quote: ... and VE-PTP mouse (1:1000 WB, 2◻μg IP, Clone 12/RPTPb, BD Biosciences).
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bioRxiv - Genomics 2019Quote: ... 902113) and Illumina Microarray Chips (HumanHT-12 v4 Expression BeadChip Kit, cat. no. BD-103-0204)
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bioRxiv - Neuroscience 2021Quote: Principal component analysis (PCA) was performed on data from 12 MEA parameters (MFR, PRS, BR, BD, BSR ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with antibodies against CD4 (clone 74-12-4, PerCP-Cy5.5-conjugated, BD Biosciences), CD8β (clone PPT23 ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were then diluted 1:1 with ice cold 0.5% BSA/CMFNM and stained with 60 μl/ml 7-AAD (BD, 559925) for > 20 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... fixed and stained with APC anti-BrdU antibody and 7-AAD using the APC BrdU Flow kit (BD Pharmingen, 552598) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Trypsinized cells were processed for BrdU-FITC and 7AAD (7-Aminoactinomycin D) staining following the manufacturer’s protocol (BD Pharmigen, 559616). FACS was run on a Scanford analyzer (Stanford FACS Core Facility ...
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bioRxiv - Cell Biology 2021Quote: ... FITC Annexin V antibodies and 7-AAD were loaded into 100 μL cells suspension aliquot to analyze pro-apoptosis following the manufacturer’s instructions (BD Biosciences). FITC isotypes were used as the isotope control ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were grown for ∼7 days and subcloned by single cell sorting on a BD FACSAria UV instrument (BD Biosciences). Thap1-/- clones were confirmed by Sanger sequencing as described above.
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bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Samples of stimulated CD45.2+ OT-I cells and CD45.1+ wild type splenocytes mixtures were stained with CD8a and CD45.2 antibodies and 7-AAD and OT-I cells were sorted (BD FACSAria) into 100% FBS and pelleted.
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bioRxiv - Microbiology 2021Quote: ... the splenocytes were stained with the extracellular markers PE-Cy ™ 7 Hamster Anti-Mouse CD3e (Cat. # 552774, BD Pharmingen), APC-Cy7 Rat Anti-Mouse CD4 (Cat ...
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bioRxiv - Genetics 2020Quote: ... we also collected 5-7 ml of peripheral blood in a tube containing the anticoagulant K3 EDTA (#6457, BD Biosciences) which was used for flow cytometry as described below ...
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bioRxiv - Genomics 2021Quote: Each strain was cultivated for 3-7 days in 10 mL of Tryptic Soy broth (BD Diagnostic, catalog no. 211768) with 0.5% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-F4/80-PE clone REA126 and human anti-CD14-FITC from Miltenyi Biotech and 7-AAD staining solution from BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... The cell viability dye 7-AAD (Via-Probe) and Live/dead fixable Aqua fluorescent reactive dye were purchased from BD Biosciences and ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Images were analysed using Cellprofiler80 with default settings to quantify the cytoplasmic to nuclear intensity ratios.The following antibodies were used for IF: p53 (DO-7 FITC conjugate, BD biosciences), USP28 (Bethyl A300-898A) ...
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bioRxiv - Cell Biology 2022Quote: ... cultured PAECs were incubated with 2’,7’–dichlorofluorescin diacetate (DCFDA; 1μM) for 10 minutes at 37°C and then assessed by flow cytometry (BD LSRFortessa).
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bioRxiv - Immunology 2022Quote: ... HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml, BD Biosciences) and CD28 (1 μg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were then diluted 1:1 with ice cold 0.5% BSA/CMFNM and stained with 60 µg/ml 7-AAD (BD, 559925) for more than 20 min on ice ...
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bioRxiv - Immunology 2024Quote: ... at which point they were phenotyped (FOXP3-PeCy7, clone FJK-16s, Thermo Fisher Scientific; HELIOS-PEDazzle594, clone 22F6, Biolegend; Thy1.1-BV711, clone OX-7, BD Biosceinces ...
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bioRxiv - Cancer Biology 2024Quote: NF1-proficient and NF1-deficient engineered clones were injected into the flanks of 6- to 7-week-old athymic nude mice (1.0×106 cells) in 50% Matrigel (BD Biosciences). Mice were monitored for tumor formation for 6 months post injection or until a tumor volume of 2000mm3 was reached.
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bioRxiv - Bioengineering 2023Quote: ... Mice sera were collected retro-orbitally at 4 h and 7 days post administration and subjected to cytokine analysis (Cytometric Bead Array Mouse Inflammation Kit, BD) using flow cytometry following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Cells were plated in a 12-well tissue culture plate (BD Falcon, BD Biosciences, San Jose, CA) for 12 hours ...
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bioRxiv - Cancer Biology 2019Quote: Cells were plated in a 12-well tissue culture plate (BD Falcon, BD Biosciences, San Jose, CA) for 12 hours ...
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bioRxiv - Immunology 2021Quote: ... Cells were labelled with a PE-conjugated Thy1.1 antibody (Clone HIS51, BD, Cat No. 12-0900-81) at a final concentration of 0.25mg/ml in MACS buffer ...
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bioRxiv - Neuroscience 2019Quote: ... As only a small number of control participants developed a mood disorder (n = 12, including 2 BD), these participants were not included in the main analysis (see Supplemental Results 3).
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bioRxiv - Immunology 2020Quote: ... cells were fixed with BD Cytofix/Cytoperm™ fixation buffer (BD Biosciences; 12 min at 37 °C) and thereafter stained with anti-p38 MAPK-AF647 (BD Phosflow ...
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bioRxiv - Cell Biology 2023Quote: ... The single cell suspension was stained in polystyrene round-bottom 12 x 75 mm tubes (BD Falcon). Primary HT1-56 (Terrace Biotech ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were resuspended in ice cold PBS and supplied with 10 μl of 7-AAD to assess cell viability (BD Pharmigen) and sorted with a BD Aria III FACS as previously described 30 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Adherent and suspension cells of each sample were pooled and after centrifugation for 5min at 800 x g cells were carefully resuspended in cell culture medium with 10 µl/ml 7-AAD (BD Biosciences). After 10 minutes incubation in the dark cells were immediately analyzed by flow cytometry using a FACS Aria III (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... washed twice and resuspended in 200 μL of blocking buffer in the presence of 7-AAD (BD Biosciences Cat#: 51-68981E) or DAPI (Invitrogen Cat# ...
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bioRxiv - Cell Biology 2022Quote: ... Cell survival was measured by FACS analysis after staining cells with a membrane-impermeable DNA binding dye (DAPI, PI, or 7-AAD) and/or annexin V (BD Pharmingen).
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bioRxiv - Developmental Biology 2022Quote: ... Cells from mouse tail skin (aging RNA-seq) and dorsal/ventral skin (fibulin 7 RNA-seq) were isolated using FACS Aria flow cytometer (BD Biosciences). Data were analyzed using FlowJo software (BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... Myoblasts were stained with 7-AAD (cell viability) and with the positive selection marker anti-CD56 AF647-conjugated (clone R19-760, BD biosciences). Of note ...
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bioRxiv - Immunology 2020Quote: ... The cells were then stained with 7-AAD (live/dead marker) prior to flow cytometric sorting on an FACS Aria II sorter (BD Biosciences). The sorted germinal centre B cells (7-AAD−/B220+/GL-7+/Fas+ ...
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bioRxiv - Immunology 2020Quote: ... A fixable Viability Dye (APC-eFluor780 1:200, 1:400) (eBioscience, Germany) or ViaProbe (7-AAD, 1:33) (BD Biosciences, Germany)) was used for live/dead discrimination ...
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bioRxiv - Immunology 2021Quote: ... and then labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, FITC anti-mouse CD4, APC-Cy®7 mouse anti-CD8, all from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: Mononuclear cells were directly labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, clone, FITC anti-mouse CD4, APC-Cy®7 anti-mouse CD8, for T lymphocyte phenotype, all from BD Biosciences ...
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bioRxiv - Genomics 2020Quote: ... ODInCas9 system was left to turn off for 7 days post transfection before assessment using FACS instrument BD LSRFortessa (BD Bioscience) and confocal microscopy instrument Cell Voyager 7000S (CV7000S ...