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Citations for Becton, Dickinson and Company :
3901 - 3950 of 3978 citations for Recombinant Mouse Cdnf His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... was used as primary antibody for samples and mouse anti-clathrin heavy chain clone 23 (1:2,000; BD Biosciences, Franklin Lakes, NJ, USA) for the detection of clathrin heavy chain as a loading control ...
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bioRxiv - Bioengineering 2024Quote: ... and stained at room temperature for 1 hour using a PE labeled mouse anti-IDO1 antibody (12-9477-42, Thermo, Waltham, MA) or a BV786 labeled mouse anti-human HLA-DR antibody (564041, BD, Franklin Lakes, NJ) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and samples were incubated in the 9-well plates with 100 μL per plate overnight at 4°C: CtBP2 mouse IgG1 (BD Biosciences 612044; RRID:AB_399431), GluA2 mouse IgG2a (Millipore MAB397 ...
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bioRxiv - Immunology 2023Quote: ... and the cells were stained with antibodies specific for the following proteins: CD4 (PE Rat Anti-Mouse CD4; Clone RM4-5; BD Biosciences; Cat # 553049), H-2Dd (Anti-mouse H-2Dd antibody ...
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bioRxiv - Microbiology 2023Quote: ... and IL-18 were measured in mouse colonic tissue extracts by using the BD OptEIA ELISA System (BD Biosciences, San Jose, CA, USA). All assays were performed as per the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... cells were detached using TrypLE™ Express Enzyme (1X), no phenol red, and stained with anti-CD31 (APC, Anti-Mouse) (BD Pharmingen™) MHCI (A488 ...
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bioRxiv - Cancer Biology 2023Quote: Epithelial cell suspensions were stained for viability using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (Thermo #L23105) and BUV737 Rat Anti-Mouse CD45 Clone 30-F11 (BD Biosciences # 568344) and analyzed using a 5-laser Cytek Aurora.
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bioRxiv - Cell Biology 2023Quote: ... and interleukin-2 cytokine production was measured using BD optiEA Mouse IL-2 ELISA set following the manufacturer’s instructions (BD Biosciences, Cat no. 555148).
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bioRxiv - Synthetic Biology 2023Quote: ... 5-μm sections were stained with the following antibodies following citrate antigen retrieval and blocking with 10% normal donkey serum: mouse anti-glutamine synthetase (BD Bioscience, 1:2000), mouse anti-Ki67 (BD ...
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bioRxiv - Immunology 2023Quote: ... 250,000 splenocytes were plated per well to perform an IFN-gamma ELISPOT per protocol using a Mouse IFN-gamma ELISPOT Kit (BD Biosciences; cat. #551083). Each sample was plated in triplicate with peptide or without peptide as a negative control ...
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bioRxiv - Developmental Biology 2023Quote: ... pre-incubated with anti-mouse CD16/CD32 (FcRII block) and then stained with the following anti-mouse monoclonal antibodies: GR1 FITC (clone RB6-8C5, BD Biosciences, RRID: AB_394643), F4/80 FITC (clone BM8 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed in perm buffer (PBS, 0.5% BSA, 0.1% Saponin) and stained for IFN-γ (APC rat anti- mouse IFN-γ; 1:100; BD Biosciences, Cat. 554413) and TNF (PE rat anti-mouse TNF alpha ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
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bioRxiv - Genomics 2024Quote: ... 2.5 x 105 cells were stained for 75 minutes at room temperature with PE Mouse anti-PDX1 Clone-658A5 (BD Biosciences; 1:10) and Alexa Fluor® 647 Mouse anti-NKX6.1 Clone R11-560 (BD Bioscience ...
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bioRxiv - Microbiology 2024Quote: ... The homogenized cell suspension was washed and resuspended in FACS buffer containing the PE-conjugated Rat anti-mouse F4/80 antibody (BD Horizon, Cat-565411) or eFluor450-conjugated anti-mouse CD11c antibody (eBioscience) ...
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bioRxiv - Developmental Biology 2024Quote: ... slides were processed as described previously for section immunofluorescent staining for detection of mouse IgG2a anti-E-cadherin (1:500, BD Biosciences, Cat# 610181) and imaged on a Leica SP5 upright confocal ...
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bioRxiv - Immunology 2024Quote: ... CD4+CD25- T effector cells were isolated from the Treg-depleted fraction of splenocytes using Anti-Mouse CD4 Magnetic Particles – DM (BD Biosciences, CA, USA). DCs were isolated from total splenocytes using CD11c microbeads according to the manufacturer’s protocols (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... were plated in buffer (PBS with 0.5% bovine serum albumin and 1 mM EDTA) and non-specific Fc binding was blocked via incubation with mouse anti-rat CD32 (BD Biosciences #550271; 1:200) for 30 minutes on ice ...
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bioRxiv - Developmental Biology 2021Quote: Mouse ESCs were pre-differentiated to an epiblast-like state as previously described [2] by seeding fibronectin-coated dishes (BD Biosciences cat. no. 356008) at a density of 0.8× 104 cells per cm2 in NDiff227 media (Takara cat ...
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bioRxiv - Microbiology 2021Quote: ... and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution, BD Pharmingen, cat No. 559864). Cell nucleus was stained with DAPI ...
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bioRxiv - Neuroscience 2021Quote: ... Synaptic ribbons were labeled with anti-CtBP2 (1/200 Goat polyclonal, Santa Cruz, USA ; cat # SC-5966 or 1/200 Mouse IgG1 monoclonal antibody BD Biosciences, cat # 612044). Otoferlin was labeled with a mouse monoclonal antibody (1/200 AbCam ...
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bioRxiv - Neuroscience 2020Quote: ... Cochlear whole mounts were immunostained overnight at 4°C with primary antibody against CTBP2 (mouse anti-CTBP2 at 1:200; BD Transduction Labs, Heidelberg, Germany). Secondary antibody (donkey anti mouse conjugated with Cy3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated overnight at 4°C in solution containing 1% BSA and primary antibodies: mouse F4/80 (T45-2342, BD Biosciences, New Jersey, USA) 1:200 ...
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bioRxiv - Immunology 2020Quote: The following rat anti-mouse antibodies were used for leukocyte analysis within each tissue: purified CD16/CD32 Fc blocking antibody (clone 2.4G2; BD Pharmingen™, San Jose, CA), PerCP-Cy™5.5 Ly6G antibody (clone 1A8 ...
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bioRxiv - Neuroscience 2020Quote: ... The transfer of the retinal suspension onto panning plates bound with monoclonal mouse anti-SSEA-1 (CD15)(MC480 clone) (#BD560079; BD Pharmingen, San Jose, CA) and monoclonal mouse anti-HNK-1/N-CAM (CD57)(VC1.1 clone ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C with a cocktail of mouse anti-human antibodies: CD19 Alexa 700 (HIB19, BD Biosciences, San Jose, CA), CD21 BV421 (B-ly4 ...
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bioRxiv - Immunology 2019Quote: ... IL-10 and IL-17α were determined in plasma samples through the Mouse Th1/Th2/Th17 Cytometric Bead Array kit (BD Biosciences, San Jose, CA). Bead fluorescence was measured using the BD Biosciences LSRII flow cytometer and analyzed with the accompanying software ...
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bioRxiv - Immunology 2020Quote: ... The following primary antibodies were used: AF-488 anti-rat CD3 (Biolegend. Clone 1F4, PE-Cy5 Mouse anti-rat CD4 (BD bioscience. Clone Ox-35), PE Mouse anti-rat CD8α (BD bioscience ...
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bioRxiv - Immunology 2020Quote: We labeled each group of NALT cells separately with the Sample-Tags from the BD Mouse Immune Single-Cell Multiplexing Kit (BD Biosciences, San Jose, CA), described in “Single Cell Labeling with the BD Single-Cell Multiplexing Kits” protocol ...
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bioRxiv - Physiology 2021Quote: ... A 3% BSA-0.2% Triton®X-100 solution was used for permeabilization and blocking followed by a 1 h RT incubation with PBS–1% BSA containing mouse monoclonal DRP1 antibody (611113, BD Transduction Laboratories, 1:50) followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121 ...
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bioRxiv - Cancer Biology 2021Quote: ... Negative cells were sorted for the absence of PD-L1 by staining with a PE-labeled anti-mouse PD-L1 antibody (clone MIH5, BD Biosciences, Franklin Lakes, NJ) using a FACSAria III (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... LN staining: macrophages were detected using a primary antibody against CD11b (1/1000, rat anti mouse CD11b conjugated with PE; BD Biosciences, San Jose, California), B-cell follicles were identified using rat anti mouse B220 Alexa F647 (1/1000 ...
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bioRxiv - Immunology 2020Quote: IgG subclasses were measured from day 21 sera using the same ELISA protocol with the following secondary Abs: goat anti-mouse IgG1-HRP (BD Biosciences, San Jose, California), goat anti-mouse IgG2b-HRP (1:1000 ...
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bioRxiv - Immunology 2022Quote: The levels of cytokines in cell culture medium were determined with BD OptEIA™ Mouse IL-6 ELISA set (BD Biosciences, Franklin Lakes, NJ), ELISA MAX™ Standard Set Mouse IL-10 (BioLegend) ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... CD45+ cells were depleted with mouse CD45 microbeads from Miltenyi Biotech(Auburn, CA) and stained with anti-Ter119-PE (BD Biosciences, San Jose, CA), anti-CD45-FITC ...
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bioRxiv - Bioengineering 2022Quote: ... Macrophages were characterized for presence and absence of canonical markers using flow cytometry PE-Cy5 mouse anti-rat CD45 (BD Biosciences, Mississauga, ON, Canada), BV786 mouse anti-rat CD11b/c (BD Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... Membranes were washed 5x times with TBS-T and stained overnight at 4°C with gentle agitation with primary antibodies: mouse anti-rat CD63 (1:200 dilution, BD Biosciences, Mississauga, ON, Canada), rabbit anti-rat CD9 (1:500 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... The phosphorylation-SMAD2/3 ability of CAR T cells was detected using PE Mouse anti-Smad2/Smad3 antibodies (clone O72-670, BD Biosciences, Franklin Lakes, NJ) following the staining protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... double-stranded oligonucleotides encoding short hairpin RNAs (shRNAs) targeting the mouse E-cadherin mRNA were annealed and then cloned into the pSIREN-RetroQ retroviral vector (BD Biosciences; Franklin Lakes, NJ). The shRNA targeting sequence for E-cadherin was 5’-TACATCCTTCATGTGAGAGTG-3’ ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed with PBS+FBS and incubated with the monoclonal antibody anti-mouse CD8 T conjugated to Fluorescein Isothiocyanate (FITC) (BD-Pharmingen, San Diego, CA), which was suitable for determining the phenotype of CD8+ T cells ...
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bioRxiv - Bioengineering 2023Quote: Splenocytes from 125Tg B6 and WT B6 control mice were stained with CellTrace Violet and cultured for 3 days35 in complete RPMI alone or stimulated with 5 ug/mL anti-mouse anti-CD40 (BD Pharmingen clone HM40-3) in the presence or absence of conjugates described above at 150 nM concentrations ...
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bioRxiv - Cancer Biology 2023Quote: CD4+ and CD8+ cells were isolated from spleens of RD and IF tumor bearing mice using BD-IMAG anti-mouse CD4 and CD8 magnetic particles (BD Biosciences, San Jose, CA) and were plated at a density of 7 x105 cells/well in cell-tak coated XFe 96 cell plates ...
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bioRxiv - Cancer Biology 2023Quote: CD8+ and CD4+ T-cells isolated from spleens of naïve mice using BD-IMAG anti-mouse CD4+ and CD8+ magnetic particles (BD Biosciences, San Jose, CA, USA) were cultured in 96-well plates (Nunc ...
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bioRxiv - Developmental Biology 2023Quote: ... before the cell pellet was resuspended in 100µl Fc block (mouse BD Fc Block CD16/CD32 Clone 2.4G2, Cat#: 553141, BD Pharminogen, 1:100 in FACS buffer) and incubated on ice for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 30 min (RT) and incubated overnight (4 °C) with mouse anti-5-LOX (1:1000; # 610695; Lot: 9067817; BD Biosciences, Franklin Lakes, NJ) and rabbit anti-perilipin-2 (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed with phosphate buffer three times for 10 minutes each and incubated with primary antibody (rat anti-mouse CD31, BD Biosciences, 550274, 1:100) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... blocked with 2% BSA in phosphate buffer pH=7.4 for 30min at RT and incubated with mouse anti-human LAMP1 (BD Biosciences, Franklin Lakes, NJ, USA) for 1h at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... bovine serum albumin in PBS for 45 min at 37°C was followed by incubation with a primary antibody mix of anti-BrdU detecting IdU (mouse, #347580, BD, Franklin Lakes, NJ, USA) and anti-BrdU detecting CldU (rat ...
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bioRxiv - Immunology 2024Quote: ... according to the protocol from vendor and stained with intracellular cytokine specific antibodies Rat anti-mouse Rorγt- Brilliant Violet-650 (BD Biosciences, #564722, clone Q31-378), mouse anti-GATA3 Alexa Fluor F488 (BD Bioscience #560163 ...