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Citations for Becton, Dickinson and Company :
3901 - 3950 of 4109 citations for Aprataxin And PNKP Like Factor APLF Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and with either CSF-1R blocking antibody or isotype control antibody at 50 ng/mL (cat# NBP1-43363, clone AFS98, Novus Biologicals; IgG K isotype control cat# 554682, BD Pharmingen). After 24 hours incubation ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then immuno-stained 1 hour at room temperature with a panel of antibodies: anti-CD3 (563725, BD, Franklin Lakes, NJ), anti-TNFα (Biolegend 502938) ...
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bioRxiv - Cell Biology 2022Quote: ... labelled with cell hashing antibodies and isolated by FACS (all steps at 4°C): Cell suspensions were incubated with Fc-block (1:50, BD Pharmingen 553142) for 10min and subsequently incubated with mouse CD45 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2023Quote: ... at 10 × 106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences, US) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% milk in 0.2% Tween-20 in TBS for 1 h at room temperature and probed with primary antibodies against SORLA (1:4000; 611680, BD Transduction Labs) and GAPDH (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... Capture and detection antibodies for human IL-6 were from the OptEIA set for human IL-6 (Cat# 55220, RRID: AB_2869045; BD Biosciences, Heidelberg, Germany) and capture and detection antibodies for human TNF were from the OptEIA set for human TNF (Cat# 555212 ...
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bioRxiv - Microbiology 2023Quote: ... and capture and detection antibodies for human TNF were from the OptEIA set for human TNF (Cat# 555212, RRID: AB_2869042; BD Biosciences, Heidelberg, Germany). Signals were detected by Streptavidin-PE (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... VE-cadherin was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD144 (BD Pharmingen™), 2 min prior to imaging ...
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bioRxiv - Immunology 2023Quote: ... all cells were harvested from the culture plates and the cell Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). Then ...
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bioRxiv - Cell Biology 2023Quote: ... T cells with residual target protein expression were depleted by antibody staining and bead pull-down or cell sorting (BD Aria III). T cells were re-suspended in MACS buffer (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cell lines were evaluated for expression of B7-H3 using B7-H3 antibody (clone 7-517, BD or clone FM276, Miltenyi).
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bioRxiv - Cell Biology 2022Quote: ... 100,000 PBMCs that had been dissolved and quantified in each media were stained with antibodies for CD3 (APC-H7, BD Biosciences (BD)) ...
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bioRxiv - Immunology 2022Quote: ... The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA) with the following antibodies/reagents (all 1:200 diluted) for 30 min on ice: anti-CD20-PE-Cy7 (BD Biosciences, 335828), anti-CD14-APC-eFluor 780 (Thermo Fischer Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5% goat serum) for 1h at RT and then stained with EC specific CD31 antibody (BD biosciences, 550274, 1:25 dilution) or muscle base membrane laminin antibody (Sigma ...
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bioRxiv - Immunology 2023Quote: ... and plated in round-bottom 96-well plates at 1-2 x 106 cells per well in complete RPMI (cRPMI) medium containing CD28 costimulatory antibody (BD Biosciences #553294) and brefeldin A ...
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bioRxiv - Immunology 2023Quote: Splenocytes-or BM-derived cells were resuspended in sPBS and the Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). After blocking ...
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bioRxiv - Genomics 2023Quote: Trypsinized or MACS sorted cells were incubated at 4°C for 20 min with APC-conjugated anti-CD56 antibodies (1:40, BD Pharmingen #555518) or isotype control (1:40 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained using fixable live/dead stain for 20 minutes at room temperature and then stained with surface-expressed antibodies (Table S2) diluted in FACS buffer and 1X Brilliant Stain Buffer (BD Biosciences, USA) for 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained with anti-Annexin V antibodies and propidium iodide (PI, DNA marker dye) as per protocol supplied with kit (BD biosciences; 556547). Flow cytometry analysis was performed for annexin V+/PI-(apoptotic ...
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bioRxiv - Immunology 2022Quote: ... with the following antibodies/reagents (all at 1:200 dilution) for 30 min on ice: anti-CD20-PE-Cy7 (BD Biosciences, 335828), anti-CD14-APC-eFluor 780 (Thermo Fischer Scientific ...
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bioRxiv - Genetics 2023Quote: Fluorescently conjugated antibodies for cell surface staining (Supplementary Table 1) were prepared in Brilliant Stain Buffer (Cat. no. 556349, BD Biosciences, Australia) and incubated with cells in a 96 well plate (Cat ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then stained at a 1:50 dilution with antibodies to immune cell targets as follows: CD3- FITC (BD Biosciences, 555332), CD8-PE (BD Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... and stained with the following monoclonal anti-mouse antibodies as described in results: GR1 FITC (clone RB6-8C5, BD Biosciences, RRID: AB_394643), F4/80 FITC (clone BM8 ...
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bioRxiv - Immunology 2023Quote: ... For the negative enrichment we used the same antibodies as for the staining but PE-labeled (Biolegend, Cat. 328109, 334105, 303105, 313203, 343605, 304058, 337004 and 305817; BD, Cat. 561433). After negative enrichment ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... Permeabilization was followed by blocking cells using 5% BSA in HBSS+ for 30 min and incubated with the primary antibody solution - Mouse anti-human CD144 (1:150; 555661, BD Biosciences, USA) for overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Blots were probed for 1h at room temperature (RT) with a mouse anti-GFP antibody (Living Colors -JL-8, BD Biosciences Clontech) in TBS-T buffer (20 mM Tris ...
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bioRxiv - Immunology 2023Quote: The Antibody mix was supplemented at 10 uL with BD Horizon™ Brilliant Stain Buffer Plus (BD Biosciences, Franklin Lakes, NJ, USA) to minimize staining artifacts commonly observed when several BD Horizon Brilliant dyes are used ...
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bioRxiv - Bioengineering 2023Quote: ... for 20min at 4°C and stained with intracellular antibodies including anti-mouse IFN-γ-Pe-Cy7 and IL-2-BV605 (BD Bioscience) for 30min at 4°C ...
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bioRxiv - Genomics 2023Quote: ... or with the appropriate class control antibodies: PE Mouse anti-IgG1 κ R-PE Clone MOPC-21 (BD Biosciences; Catalog no. 559320) and Alexa Fluor® 647 Mouse anti IgG1 κ Isotype Clone MOPC-21 (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used in this study: rabbit anti-AP-4 epsilon 1:400 for Western blots (612019; BD Transduction Labs); rabbit anti-ATG9A 1:200 for immunofluorescence and 1:1000 for Western blots (ab108338 ...
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bioRxiv - Immunology 2024Quote: ... three LNs (for patients ccp2 and ccp3) and two replicates of PBMC were stained with a panel of fluorescent antibodies: CD45-PerCP/Cy5.5 (BD 564105, clone HI30), CD38-PE (BD 555460 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated with anti-mouse CD32 for 5 min at 4°C to block Fc receptors and stained with fluorochrome conjugated antibodies to CD45R/B220 PE (1:200) (clone RA3-6B2, BD Bioscience #553089), Ter119 PE (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Unspecific binding of antibodies was blocked by incubating the samples with 3% rat serum in BD staining buffer (BD Biosciences, Heidelberg, Germany) for 5 minutes at RT ...
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bioRxiv - Immunology 2024Quote: ... Content of chemokines / cytokines was quantified after washing and staining with biotinylated detection antibodies and phycoerythrin bound streptavidin by using LSR II (BD Bioscience, Germany). Calculation of each chemokine / cytokine quantity was determined by using standard curves according manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: Intracellular staining for BIM was performed with secondary Donkey anti-rabbit APC secondary antibody using BD cytofix/cytoperm kit (BD biosciences 554722) per manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... The cells were then incubated for 1 h with primary antibodies directed against the cis-Golgi marker GM130 (mouse anti-GM130, BD bioscience, 610822) and/or trans-Golgi marker TGN46 (sheep anti-TGN46 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed and then stained extracellularly with antibodies (Table S1) diluted in 0.5% FACS buffer and brilliant staining buffer (BD Biosciences, Cat# 563794) for 20 mins at room temp ...
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bioRxiv - Cell Biology 2024Quote: ... They were stained for 2 hours at room temp with the following primary antibodies: mouse anti-GM130 (1:250, BD Bioscience, BDB610822) and rabbit anti-TGN46 (1:500 ...
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bioRxiv - Immunology 2024Quote: ... cells were suspended in 0.5 mL PBS+3% FBS and stained for flow cytometry analysis by adding anti-CD16/CD32 antibody (BD Pharmingen; clone 2.4G2) ,anti- CD117 (Biolegend clone 2B8) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were blocked with Superblock blocking buffer in PBS/ 0.1% Triton-X for 1 h and then incubated with the following primary antibodies overnight at 4 °C: α-CD11b (1:100; BD Biosciences) and α-Iba1 (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... PE mouse anti-human VCAM-1 or Alexa Fluor 647 mouse anti-human CD31 (all antibodies were from BD, Franklin Lakes, NJ, USA). Samples were analyzed on Gallios Flow Cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... The antibodies used for the flow cytometry analysis of the cell surface markers were: anti-human CD11b (740965, BD OptiBuild™, 1:100), anti-human CD14 (11-0149-42 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with a fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD31 antibody (1:50; BD Pharmingen, San Diego, CA, USA) for 30 min at room temperature followed by 3 washes with phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: Eosinophils of 6-12 week-old Il5-tg females and males were positivelyenriched using PE anti-mouse Siglec F antibody (562068 BD Biosciences; E50-2440) and anti-PE microbeads (130-042-401 Miltenyi Biotech) ...
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bioRxiv - Immunology 2021Quote: ... Zap70 and CD49d were evaluated by flow cytometry (antibodies in Table S2) on an LSR II flow cytometer (BD Biosciences, Franklin Lakes, NJ). Mutational status of the expressed BCR was determined by PCR from PBMC RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated for 60 minutes in 1% BSA and 5% goat serum in PBS and then incubated overnight with anti-p21 antibody (BD Pharmingen, mouse, #556431) and anti-p16 antibody (abbiotec ...
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bioRxiv - Molecular Biology 2020Quote: ... Edited and non-edited cells were analyzed by flow cytometry using the following antibodies: hCD45 V450 (HI30; BD Biosciences, San Jose, CA, USA), CD34 APC (561 ...
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bioRxiv - Neuroscience 2019Quote: ... injection with α-Ly6G or isotype control antibodies (IP 4 mg/kg; α-Ly6G, clone 1A8, No. 561005; Iso-Ctr, Rat IgG2a, κ, No. 553929; BD Biosciences). Animals were 9-10 months of age at the start of the experiment.
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bioRxiv - Systems Biology 2021Quote: ... Cells were then left at 4°C overnight in a blocking solution containing the primary antibody: mouse E-Cadherin (BD Biosciences, 1:1000) and mouse N-Cadherin (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for ON in primary antibody (SOX2, R&D Systems, MAB2018; TBR2, Abcam, ab23345; HOPX, Santa Cruz, sc398703; HOPX, Proteintech, 11419-1-AP; N-cadherin, BD Biosciences ...