Labshake search
Citations for Becton, Dickinson and Company :
3801 - 3850 of 4199 citations for Recombinant Mouse Prl Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... the cell surface of freshly-prepared epidermal cells was labeled with the following antihuman protein antibodies (clone, manufacturer): FITC–conjugated CD1a (HI149) and PE–conjugated HLA-DR (G46-6, both BD Pharmingen), PE–CF594–conjugated CD3 (UCHT1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Transduced cells were selected with 5μg/ml blasticidin and bulk cells expressing the TetR protein were clonally isolated in 96-well plates using FACS (BD-Aria). BRAFV600E and BRAFWT were expressed in cells using Gateway-compatible lentiviral constructs (pLenti CMV/TO puro DEST ...
-
bioRxiv - Microbiology 2021Quote: ... The lung infiltrated cells were cultured with purified RBD protein at 10 μg/ml for 12 h at 37 °C followed by a 6 h treatment with GolgiPlug (BD 555028). After blocking with FcγIII and FcγII receptors antibody (BD Pharmingen ...
-
bioRxiv - Immunology 2020Quote: ... CD95, CD278) or intracellular proteins (BCL-2, BCL-6, CD152) and cells were acquired on an LSR II analyser (BD Pharmingen), followed by flow cytometric analysis using the FlowJo Software (FlowJo LLC ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then cultured for 2 hours at 37°C in 5% CO2 incubator and followed by the addition of protein secretion inhibitor cocktail containing brefeldin A and monensin (GolgiPlug, BD, 555029) for another 4-hours ...
-
bioRxiv - Immunology 2022Quote: ... Spike and RBD tetramers were prepared fresh before use and formed by combining biotinylated proteins with PE-conjugated Streptavidin (BD Biosciences) at a molar ratio of 4:1.
-
bioRxiv - Neuroscience 2022Quote: ... that was blocked in 5% non-fat milk for 1 h at room temperature and incubated overnight with primary antibodies against the following proteins: α-synuclein (1:1000, BD), NCK2 (1:1000 ...
-
bioRxiv - Immunology 2022Quote: ... Protein transport inhibitor containing Monensin and protein transport inhibitor containing Brefeldin A were added to cells (both 10 μg/ml, BD Biosciences) and cells cultured for an additional 4 hours ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... nuclei were resuspended in 500 μl of wash buffer and labelled with an anti-nuclear pore complex proteins antibody (clone Mab414) and sorted on a FACSAria instrument (BD Biosciences), with a 100 nozzle on a cold block ...
-
bioRxiv - Cancer Biology 2024Quote: ... Transfected cells were selected with 5 mg/mL puromycin and sorted based on red fluorescent protein (RFP) by fluorescence-activated cell sorting (FACS) on a FACSAria (BD Biosciences). RFP+ cells were serially diluted in a 96-well plate to generate single cell clones ...
-
bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. The surface expression level of S proteins (Fig. 3d) was measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
-
bioRxiv - Immunology 2023Quote: ... we first labeled cell surface proteins in FACS buffer for 30 minutes before washing with 1x PBS and fixation with BD Cytofix/Cytoperm (BD Biosciences) for 15 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. The surface expression level of S proteins (Fig. 3d) was measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Phycoerythrin (PE) or peridinin chlorophyll protein -cyanine 5.5 (PerCP-Cy5.5)-conjugated CD3 (BD Biosciences Cat#5 61808, BD Biosciences Cat# 55163); Pacific Blue-conjugated (PB) ...
-
bioRxiv - Microbiology 2023Quote: ... was used as a secondary antibody. The surface expression levels of S proteins (Fig. 4A) were measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (FlowJo LLC) ...
-
bioRxiv - Neuroscience 2023Quote: Expression of the different fluorescent proteins (ORFs) of the stable GEFRI2 line were measured by High Throughput LSR Fortessa (BD Biosciences) fluorescence-activated cell sorter (FACS) ...
-
bioRxiv - Immunology 2023Quote: ... followed by staining with a cocktail of mAbs and four fluorochrome-conjugated spike proteins in staining buffer (2% FBS/PBS) supplemented with Brilliant Stain Buffer Plus (BD Biosciences). The stained cells were fixed (Lysing Solution ...
-
bioRxiv - Immunology 2024Quote: ... NK cells were either settled on substrates functionalized with NKp46 antibody or incubated with K562 cells in presence of BV421 anti-CD107a antibody and protein transport inhibitor Golgi stop (BD Biosciences) at 37°C with 5% CO2 for 4 hours ...
-
bioRxiv - Immunology 2023Quote: ... Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail, BD Biosciences) and added to the cells ...
-
bioRxiv - Immunology 2024Quote: T cells were restimulated with 3 μg/ml plate-bound anti-CD3 and 2 μg/ml soluble anti-CD28 or PMA/Ionomycin in the presence of GolgiPlug protein transport inhibitor (BD Biosciences) for 6 hours ...
-
bioRxiv - Immunology 2024Quote: ... The cells were resuspended in T cell stimulation media (RPMI 1640 supplemented with 10% FBS, 1% Penicillin/Streptomycin, Protein Transport Inhibitor (Containing Brefeldin A) (diluted 1:1000) (BD, 555029), Monensin (Biolegend ...
-
bioRxiv - Molecular Biology 2024Quote: ... Transduced pools of cells were sorted by Blue Fluorescent Protein (BFP) expression in BD FACSAria II SORP cell sorter (BD Biosciences). Gene edition was induced in mESC lines by 5-day treatment with 2 μg/ml Doxycycline (Sigma ...
-
bioRxiv - Cancer Biology 2021Quote: ... AKP-Z tumor derived cells were stained using the following antibodies: rat anti-mouse CD45-BV421 (clone 30-F11, 563890, BD Biosciences), rat anti-mouse CD31-BV421 (clone 390 ...
-
bioRxiv - Immunology 2021Quote: ... were cultured with 5E5 of YAC-1 target cells (2:1 ratio) in the presence of anti-mouse CD107a-BV421 antibody (564347, BD Biosciences) (1:100 ...
-
bioRxiv - Immunology 2021Quote: ... One million cells per sample were pre-incubated with purified anti-mouse CD16/CD32 antibody (Fc Block™, 553141, BD Biosciences). Live/dead cells were discriminated by staining cells with the LIVE/DEAD® Fixable Near-IR Dead Cell Stain kit (L10119 ...
-
bioRxiv - Immunology 2021Quote: ... MLN cell suspensions were added to a 96 well plate for surface staining with anti-mouse TCRβ-FITC (clone H57-597, BD Biosciences) and anti-CD4-PerCP-Cy5.5 (clone RM4-5 ...
-
bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight in blocking solution with the following primary antibodies (1:100): rat anti-mouse SiglecF (E50-2440, 552126 BD Biosciences), Armenian hamster anti-mouse CD80 (16-10A1 ...
-
bioRxiv - Immunology 2021Quote: ... and surface stained for 30 mins at 4°C with 50 μL/well anti-mouse CD3 APC-CY7 (clone 17A2, 1:400, BD Biosciences), anti-mouse CD4 BV510 (clone RM4-5 ...
-
bioRxiv - Immunology 2021Quote: ... the brain sections were incubated with 3% serum for 1 hour followed by over-night incubation with primary antibodies (1:1000; Rat anti-mouse CD4 from BD Bioscience) in a humidified chamber at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: Protein synthesis of Syndecan-1 was shown by immunocytochemistry using a polyclonal antibody against murine Syndecan-1 (CD138) clone 281-2 (1:50, rat anti-mouse, BD Pharmingen) in co-cultured cells after fixation with ice-cold 99% methanol and heparitinase III (2.4mIU/ml ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed twice and incubated overnight at 4°C with the fluorochrome-conjugated antibodies and unconjugated rat and mouse IgG in Perm/Wash solution (BD Biosciences), which was followed by additional 5 min incubation in Perm/Wash solution without antibodies ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were re-suspended in 100 μl of FCSB and 1 μl of mouse anti-human CD10-PE (561002, BD Biosciences) and mouse anti-human CD92-AF647 (565316 ...
-
bioRxiv - Developmental Biology 2020Quote: ... rabbit anti-Lhx1 (1:250, gift from Dr. Masanori Taira) and mouse anti-E-cadherin (1:100, BD Transduction Laboratories, 610182). For detection of primary antibodies the following secondary antibodies were used ...
-
bioRxiv - Immunology 2022Quote: BMDMs were harvested as described before and Fc receptors were blocked with an anti-mouse CD16/CD32 antibody (1:250, BD Biosciences) in FACS buffer (5 % heat-inactivated FCS ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C with the astrocyte (mouse anti-GFAP; 1:1000, BD Biosciences, San Diego, CA, USA) and microglia (rat anti-CD11b ...
-
bioRxiv - Cell Biology 2022Quote: ... 10 μl of platelet suspension was mixed with 40 μl of staining solution (HEPES-Tyrode buffer supplemented with 2.5 mM CaCl2, 1:50 PE rat anti-mouse CD41 antibody (BD Biosciences 561850), and 3:50 FITC Annexin V (BD Biosciences 556419)) ...
-
bioRxiv - Microbiology 2020Quote: ... cells were incubated with fluorochrome-conjugated (FITC, PE, PE-Cy7, allophycocyanin [APC], APC-eFluor 780,) antibodies against mouse CD45 (30-F11; BD Biosciences), Ly6C (AL-21 ...
-
bioRxiv - Cell Biology 2019Quote: ... transferred to flow cytometry tubes and stained with 1:100 mouse IgG2a CD31-647 clone M89D3 (BD Bioscience, 558094, lot 8145771) for 30 min on ice then washed once with DPBS ...
-
bioRxiv - Cell Biology 2019Quote: ... Tet1KO and WT reprogramming intermediates were incubated 30 min on ice with Phycoerythrin (PE)-conjugated Mouse anti SSEA1 (1:100, Cat.#FAB2155P-025, R&D) and analysed on CANTO I (BD Biosciences). PGCLCs at day8 were sorted using FITC Mouse anti-SSEA1 (1:20 ...
-
bioRxiv - Immunology 2019Quote: Lymphocytes or purified splenic B cells were washed in PBS with 1% BSA and incubated with 1mg/ml purified rat anti-mouse CD16/CD32 (Fc block, BD Pharmingen) at 4°C for 15 min ...
-
bioRxiv - Immunology 2019Quote: ... Bound antibody was revealed by staining with a FITC- or PE- conjugated anti-mouse IgMa (Igh-6a) monoclonal antibody (BD Pharmingen). Bone marrow progenitor B cells were defined as B220+ ...
-
bioRxiv - Immunology 2020Quote: ... and 14 days post-infection (DPI) was determined by culturing serially diluted mouse lung homogenates on 7H10 agar plates (BD Biosciences) at 37°C for 2-3 weeks ...
-
bioRxiv - Immunology 2019Quote: ... incubated with anti-mouse IgG1-fluorescein isothiocyanate for 45 min at room temperature (1:100 dilution; BD Pharmingen; cat. no. 553443), and washed three times with PBS ...
-
bioRxiv - Developmental Biology 2019Quote: ... Cell suspensions from APEL vascular differentiations were incubated with mouse anti-human CD31-APC (eBioscience, San Diego, CA) and CD146-PE (BD Biosciences) antibodies for 30 min on ice ...
-
bioRxiv - Microbiology 2019Quote: ... the cells were washed once with FACScan buffer and then stained with PE-conjugated monoclonal rat anti-mouse CD4 (BD Pharmingen) and/or APC-conjugated monoclonal rat anti-mouse CD8 (eBioscience) ...
-
bioRxiv - Immunology 2020Quote: ... cover slips were washed 3 times in 1xPBS and incubated with secondary antibodies (anti-rabbit-AF647, anti-mouse-AF488 (BD Bioscience)) diluted 1:1000 in antibody diluent for 1 hour at RT ...
-
bioRxiv - Immunology 2019Quote: ... B cells were pre-incubated during 15 minutes with 0.5 μg/ml anti-mouse CD16/CD32 (Clone 2.4G2, BD Pharmingen, ref 553142) in FACS buffer (PBS supplemented with 2% FCS and 2 mM (Ethylenedinitrilo)tetraacetic acid (EDTA ...
-
bioRxiv - Immunology 2019Quote: ... Cells were further incubated for an additional 30 minutes on ice with a mixture of the following antibodies: Alexa Fluor 700 rat anti-mouse CD45R (B220, clone RA3-6B2, BD Biosciences), FITC rat anti-mouse CD43 (Clone S7 ...
-
bioRxiv - Molecular Biology 2019Quote: ... The HLA-DQ cell surface expression was performed by cytofluorimetric analysis using the FACSAria III and DIVA software with FITC mouse anti-human HLA-DQ antibody (BD Biosciences). The quantitation of specific transcripts was performed by qRT-PCR as described in [9] ...
-
bioRxiv - Cancer Biology 2019Quote: ... cKIT and PDGFRA expression was assessed using mouse anti-human CD117 (PE-Cyanine7, clone 104D2, #25-1178-42, eBioscience) and CD140a (PE, clone αR1, #556002, BD Pharmingen) respectively ...