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Citations for Becton, Dickinson and Company :
3751 - 3800 of 3864 citations for Forkhead Box L1 FOXL1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... pre-coated for 1 hour at 37°C with anti-CD3 antibodies (5ug/mL, clone: 145-2C11, BD Biosciences, San Diego, CA, USA) and supplemented with soluble anti-CD28 antibodies (2ug/mL clone ...
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bioRxiv - Immunology 2024Quote: ... The labeled cells were cultured in complete RPMI-1640 medium plus IL-2 (20 units/ml) or IL-2 and anti-CD3 antibody (1μg/ml) (BD Pharmingen (San Diego, CA), or alternatively plus IL-4 (4ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with multiple flow cytometry validated antibody cocktails (see below) and analyzed using either BD Fortessa or LSRII cytometers (BD Horizon, BD, Franklin Lakes, NJ). Data were analyzed and quantified using FlowJo v10.6 software (RRID:SCR_008520) ...
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bioRxiv - Immunology 2023Quote: ... Surface antibodies were prepared at optimal dilutions (see Supplementary Table S1) in FACS supplemented with Brilliant Stain Plus buffer (BD; Franklin Lakes, NJ), then were added to samples in blocking solution ...
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bioRxiv - Developmental Biology 2023Quote: ... and then blocked in 1% bovine serum albumin in PBST before addition of primary antibodies against E-cadherin (1:200, BD Transduction Laboratories, 610181), laminin (1:200 ...
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bioRxiv - Immunology 2023Quote: ... All 5 samples were then pooled and stained for surface markers in 70μl of the antibody cocktail (Supplementary Table 2) in BD Horizon Brilliant Stain Buffer Plus (BD Biosciences, San Jose, CA) for 30 minutes at 4 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5-μm sections were stained with the following antibodies following citrate antigen retrieval and blocking with 10% normal donkey serum: mouse anti-glutamine synthetase (BD Bioscience, 1:2000), mouse anti-Ki67 (BD ...
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bioRxiv - Developmental Biology 2023Quote: ... pre-incubated with anti-mouse CD16/CD32 (FcRII block) and then stained with the following anti-mouse monoclonal antibodies: GR1 FITC (clone RB6-8C5, BD Biosciences, RRID: AB_394643), F4/80 FITC (clone BM8 ...
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Cancer Biology 2023Quote: ... The bottom and top parts of the membrane were then incubated overnight at 4°C with primary p53 antibody (2 µg/mL, 1:250) (Clone DO-1, BD Biosciences, Cat. 554293) and primary vinculin antibody (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 4°C for 30 min with a B cell surface marker antibody cocktail (Table S11) with 10 µl Brilliant Stain Buffer Plus (BD, cat. No. 566385) diluted in PBS containing 1% BSA up to a volume of 100 µl ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with flow cytometry antibodies (Supplemental Table 9) in flow cytometry staining buffer (PBS, 1% BSA) and brilliant staining buffer (563794, BD, Franklin Lakes, NJ). Cells were analyzed using a 5-laser (16UV-16V-14B-10YG-8R ...
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bioRxiv - Microbiology 2023Quote: ... we stained cells expressing wild-type and mutant SFV glycoproteins with a mouse anti-SFV immune ascitic fluid (ATCC Cat#: VR-1247AF) or a control mouse IgG2b antibody (BD Biosciences Cat#: 557351). Staining of wild-type and mutant EEEV or SFV E3–E2–(6K/TF)–E1 was detected with a PE-conjugated donkey anti-mouse F(ab’)2 fragment (Jackson ImmunoResearch Cat# ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% skim-milk diluted in 1X TBST and incubated with primary antibodies overnight [RB (BD Pharmingen #554136, 1:500), p53 (Pierce #MA5-12453 ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were washed with PBS and stained for 20min at 4°C with anti-EphB2 APC conjugated antibody (BD Biosciences, Heidelberg, Germany). Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec ...
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bioRxiv - Physiology 2024Quote: ... then cells were washed with 1x PBS and resuspended in FACS buffer and non-specific binding to Fc receptors was blocked by incubation with anti-CD16/32 antibody (BD Pharmingen, Cat# 553141) and the FACS antibodies BV711 conjugated anti-CD11b and Pacific blue conjugated anti-CD45 antibodies (Cat# 101241 ...
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bioRxiv - Microbiology 2024Quote: ... The homogenized cell suspension was washed and resuspended in FACS buffer containing the PE-conjugated Rat anti-mouse F4/80 antibody (BD Horizon, Cat-565411) or eFluor450-conjugated anti-mouse CD11c antibody (eBioscience) ...
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bioRxiv - Neuroscience 2024Quote: ... The cover slips were then permeabilized with 0.3% Triton X-100 and sequentially stained with rat anti-CD44 antibody (BD Biosciences, 550538; 1:200), and corresponding Alexa 488-conjugated secondary antibodies (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The antibodies used for stimulation were anti-human CD3 (clone UCHT1, plate bound) and anti-human CD28 (clone 28.2, BD Biosciences, San Jose, CA) at 1 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed with FACS buffer stained with antibodies to detect surface expression of CD4 (GK1.5) and CD25 (PC61) (BD Biosciences, Franklin Lakes, NJ), whereas intracellular expression of FoxP3+ was stained using an anti-Mouse/Rat FoxP3 (FJK-16s ...
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bioRxiv - Molecular Biology 2021Quote: ... stained with a collection of different fluorescent labelled antibodies (CD4-BV785; BioLegend, San Diego, CA, USA; CD25-PE-Cy5.5; BD Bioscience; and CD54-AF700; EXBIO) and analysed on a Cytoflex flow cytometer (Software CytExpert 2.3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Enumeration of human CD45+ cells in spleen and bone marrow was performed by flow cytometry after staining with BV605-conjugated human-specific CD45 antibody (clone HI30, BD Biosciences, San Jose, CA) using a BD LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... 50 uL cells per well were stained for 45 min at room temperature with the antibodies shown in the Key Resources Table (all reagents from BD Biosciences, San Jose, CA). Cells were washed three times with FACS buffer (PBS supplemented with 2% FBS and 0.1% sodium azide) ...
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bioRxiv - Immunology 2019Quote: ... Single-cell suspensions at 5 × 107 cells per ml were stained with a cocktail of fluorochrome conjugated human antibodies (BD Biosciences, San Jose, CA), anti-human IgG APC ...
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bioRxiv - Immunology 2021Quote: ... The DCs were phenotyped using phycoerythrin-conjugated (PE) monoclonal antibodies (mAbs) against CD83 and CD14 setting the cutoff for positive cells with the isotype control IgG2a (BD Pharmingen, Franklin Lakes, NJ). Immature DCs were transferred to new plates at a concentration of 4×105cells/mL ...
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bioRxiv - Microbiology 2021Quote: ... and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution, BD Pharmingen, cat No. 559864). Cell nucleus was stained with DAPI ...
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bioRxiv - Neuroscience 2021Quote: ... Synaptic ribbons were labeled with anti-CtBP2 (1/200 Goat polyclonal, Santa Cruz, USA ; cat # SC-5966 or 1/200 Mouse IgG1 monoclonal antibody BD Biosciences, cat # 612044). Otoferlin was labeled with a mouse monoclonal antibody (1/200 AbCam ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 4% milk for 1 hour and incubated overnight at 4°C with shaking in the appropriate primary antibody against Nischarin (1:500, BD Biosciences, Cat No: 558262), GFP (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Cochlear whole mounts were immunostained overnight at 4°C with primary antibody against CTBP2 (mouse anti-CTBP2 at 1:200; BD Transduction Labs, Heidelberg, Germany). Secondary antibody (donkey anti mouse conjugated with Cy3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated overnight at 4°C in solution containing 1% BSA and primary antibodies: mouse F4/80 (T45-2342, BD Biosciences, New Jersey, USA) 1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... The sections were washed three times with PBS and then incubated with primary antibodies anti-ASO (Ionis Pharmaceuticals. Carlsbad, CA) and anti-CD31 (BD Bioscience, San Jose, CA) with a 1:200 dilution in blocking buffer at 4 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated as described above and stained at 4°C (15 min) with fluorochrome-conjugated antibodies against surface markers of endothelial cells (CD31 (BD Biosciences, Franklin Lakes, USA) and myeloid cells (CD45 (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: qNSCs and aNSCs from the SVZ were FACS purified from GFAP::GFP mice as previously described (Codega et al., 2014) using the following antibodies: anti-mCD24 PE (1:2000; BD Pharmingen 12-0242-82), biotin conjugated anti-mCD133 (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stained with Alexa Fluor 647–conjugated anti– human CD24 and APC-H7 labeled anti–human CD44 antibodies (BD Pharmingen, San Jose, CA) at 4°C for 20 min ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C with a cocktail of mouse anti-human antibodies: CD19 Alexa 700 (HIB19, BD Biosciences, San Jose, CA), CD21 BV421 (B-ly4 ...
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bioRxiv - Cell Biology 2019Quote: ... The following day they were probed with antibodies to FLAP (Novus, IMG 3160, 1:100) and to 5-LO (BD Biosciences, 610695, RRID: AB_398018). Secondary antibodies were applied at 3 µg/mL for 1 h ...
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bioRxiv - Immunology 2020Quote: ... The following primary antibodies were used: AF-488 anti-rat CD3 (Biolegend. Clone 1F4, PE-Cy5 Mouse anti-rat CD4 (BD bioscience. Clone Ox-35), PE Mouse anti-rat CD8α (BD bioscience ...
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bioRxiv - Bioengineering 2021Quote: ... cells were stained with surface marker antibodies and analyzed with Fluorescence-Assisted Cytometry (FACs) using a BD LSR Fortessa (BD Biosciences, San Jose, CA). Propidium Iodide (5:1000 µL ...
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bioRxiv - Cancer Biology 2019Quote: Glioma TMA sections (4 μm) were stained using a rat monoclonal primary antibody to the integrin β4 subunit (CD104; clone 439-9B; BD Pharmingen, San Jose, CA) at a concentration of 5μg/ml according to a previously described protocol (11) ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the cocktail for surface staining: CD3-allophycocyanin (APC)-Cy7 (clone SP34-2, Cat#557757, BD Pharmingen, San Jose, CA), CD4-PE-CF594 (clone L200 ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... cells were centrifuged and resulting pellets were resuspended in 1:100 mixture of panel of antibodies: FITC-conjugated CD31 (BD Biosciences, Mississauga, ON, Canada), AF647-conjugated CD45 (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs were assessed to determine the frequency of circulating T follicular helper (Tfh) using a panel which included the following antibodies: CD3 (BD Biosciences; clone SP34-2), CD4 (BD Biosciences ...
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bioRxiv - Physiology 2021Quote: ... A 3% BSA-0.2% Triton®X-100 solution was used for permeabilization and blocking followed by a 1 h RT incubation with PBS–1% BSA containing mouse monoclonal DRP1 antibody (611113, BD Transduction Laboratories, 1:50) followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121 ...
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bioRxiv - Immunology 2021Quote: ... harvested and stained for flow cytometry as described (37) using antibodies to human surface antigens: CD4-APCH7 (SK3, BD Biosciences Franklin Lakes, NJ, USA), CD8-PerCP (SK1 ...
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bioRxiv - Immunology 2020Quote: ... The 3T3 cells were FACS sorted four times for expression of CD40L using an anti-CD40L antibody (clone TRAP1, BD Bioscience, San Jose, California). This resulted in a stable CD40L-expressing cell line that was cultivated in G418 containing selection media to maintain expression ...
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bioRxiv - Immunology 2020Quote: ... LN staining: macrophages were detected using a primary antibody against CD11b (1/1000, rat anti mouse CD11b conjugated with PE; BD Biosciences, San Jose, California), B-cell follicles were identified using rat anti mouse B220 Alexa F647 (1/1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were blocked in 5% BSA and probed overnight at 4°C with the following primary antibodies and dilutions: p16 (1:200, #554079 BD Biosciences, San Jose, CA), p53 (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in 100 μl of staining solution of FACS buffer containing biotinylated antibodies against CD31 (BD Biosciences, 558737, 1:100), CD45 (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1) after Fc blocking and analyzed using flow cytometry (BD FACS Celesta/Novocyte Advanteon). Cells were fixed and permeabilized using intracellular staining reagents (Intracellular Fixation & Permeabilization Buffer Set ...